BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,240,883	A→C	5.8%	F458V (TTT→GTT)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,240,883	0	A	C	5.8%	258.7 / 5.2	103	F458V (TTT→GTT)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base A (56/39); new base C (4/4); total (60/43)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 106 total aligned reads displayed.

CCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAG  >  NC_000913/4240646‑4241121

ccACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGCCAGGAAGATTACAGCGTAGCAGTTTGTTGGGTTTTTTTTGTTTCCGGTTCCCCGAGCGCTTCCAGCTCCTCCAGGG‑GTTTTCCTTTGGTTTCCGGGCCAAA‑TTTCACCAAAAAc                                                                                                                                                                                                                                                 >  2:802864/1‑239 (MQ=255)

ccACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCCGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGAAAAA‑TTTCCACATAAAc                                                                                                                                                                                                                                                 >  2:609790/1‑239 (MQ=255)

       atttgtTGGTCTACTCGGTGTCTGTCGTTTTACTGGTGATCGCCATGTGCCTTTGTAATTTGAAGGGGCTGATGAGGGAGTGAAAAATCAGGACGCCTAAATGGCGCTGCTGGTTGGACAGCAAGATTATAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGATCGCTTCCAGCTCCTCAAGGG‑TTTTCACTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGtgct                                                                                                                                                                                                                                          <  2:869287/235‑1 (MQ=255)

        tgtgTTGATTTTCCGGGCTGCGGGCCGTTTACTGGTGATCACCCTGGGGCTGGGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGtgctg                                                                                                                                                                                                                                         <  2:72088/238‑1 (MQ=255)

           gtTGATCTACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCTAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCAAATAAAC‑AGGGCTGccca                                                                                                                                                                                                                                     >  2:596902/1‑237 (MQ=255)

           gtTGATCTACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGCCAAA‑TTTCCACATAAAC‑AGTGTTGCCAg                                                                                                                                                                                                                                     >  1:227475/1‑239 (MQ=255)

            ttGATCTACTCGGCTGGGATCCTGTTACTGGTGATCACCCTGGGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAg                                                                                                                                                                                                                                     <  1:235305/238‑1 (MQ=255)

             tGATCTACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGaa                                                                                                                                                                                                                                   <  1:451738/239‑1 (MQ=255)

                  tACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGACAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGGGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCCGCTCCTCAAGGG‑TTTTAACTTTGTTTTCCGGGGCAAA‑TTTCCCAATAAAA‑AGGGCTGCCAGAACGccc                                                                                                                                                                                                                              >  2:465630/1‑239 (MQ=255)

                      cGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGACGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGGGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCCACTCCAGGG‑TTTTACCTTTTGTTTCCGGGACAAA‑TTTCCACATAAAA‑GGTGCTGCCAAAACGccacaac                                                                                                                                                                                                                          >  2:429995/1‑234 (MQ=255)

                         cTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATGACAGCGTAGCAGGTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑GTTTACCTTTGGTTTCCGGGACAAA‑TTTCACCATAAAC‑ATGGCTGCCAGAACGCCCAtcaaa                                                                                                                                                                                                                        >  2:609313/1‑234 (MQ=255)

                                   gTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑Ca                                                                                                                                                                                                            >  1:443396/1‑239 (MQ=255)

                                    ttACTGGGTATCCCCCTGTGGCTGTGTAATTCGAAACGGCTGAAACGGGAGTAAAATGGCAGCGCGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAg                                                                                                                                                                                                           <  1:385104/239‑1 (MQ=255)

                                          gTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAACA‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGgag                                                                                                                                                                                                     >  1:641647/1‑239 (MQ=255)

                                          gTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGATTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACCCCCATACAAcc                                                                                                                                                                                                                      >  1:618453/1‑223 (MQ=255)

                                            gatccaccTGGGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGgaga                                                                                                                                                                                                    <  1:72819/232‑1 (MQ=255)

                                            gATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGAAGGGGaa                                                                                                                                                                                                   >  1:738189/1‑239 (MQ=255)

                                            gATCACCCTGGGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCCAAATGGCGCGGCGTGCTGGCCAGGAAGATTACAGCGTAGCCGTTTGTTGTGTTTTCTTCGTTCCCGGTTCCCAGATCGCTTCCAGCTCCTACAGGG‑TTTTACCTTTTGTTTCCGGGCACAA‑TTTCCACTTAAAC‑AGAGCTGCCAGAACGCCCATACAACCAGAAATc‑cccttggaaac                                                                                                                                                                                                   >  2:533065/1‑229 (MQ=255)

                                             aTCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGGAAGGAAGATTACAGCGTAGCAGTTTGTTGGGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGCCAAA‑TTTCCCCATAAAC‑AGTGCTCCCAGAACCCCCATCCAACGGTAAATcaggaaggggaa                                                                                                                                                                                                    >  2:579087/1‑227 (MQ=255)

                                             aTCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGaa                                                                                                                                                                                                   >  1:549680/1‑238 (MQ=255)

                                              tccccttgtgGGTGTGTAATTCGAAAGGGTGGAAGCGGGAGAAAAAAGGAAGGACGCCGAAAGGGCGGGGGTTCTTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGaaa                                                                                                                                                                                                  <  2:811593/232‑1 (MQ=255)

                                              tCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGCAGGAGaaa                                                                                                                                                                                                  >  1:637342/1‑238 (MQ=255)

                                                    tgtgGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACCGCGAAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGCG‑TTTTACCTTTGGTTTCCGGGCCAAA‑TTTCCACATAAAC‑ATGGCTGCCAGAACGCCCATACAACCGTAAAAC‑CAGAAGGAAAAACCgggtg                                                                                                                                                                                           >  2:304724/1‑235 (MQ=255)

                                                    tgtgGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAATGG‑TTTTACCTTTTGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCGGACAGAACCCCCATACAACCCTAAATC‑CAGCAGGGAAAAcggggtg                                                                                                                                                                                           >  2:780833/1‑233 (MQ=255)

                                                          tgtgTAATTCGAAACGGCTGGAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGGGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGaaa                                                                                                                                                                                      <  1:778828/238‑1 (MQ=255)

                                                            tgtAATTCGGAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGTAGATTACAGCGTAGCCGTTTGTTGTGTTTTCTTGATTACCGGTTCCCAGAGCCCTTCCAGCTCCTCAAGGG‑TTTTACCTTTTGTTTCACGGACAAA‑TTTCCAACAAAAC‑AGAGTTGCCCGACCGCCCATTATAACGTAAACC‑CACTAGGAAAAAACGTTgggggacgag                                                                                                                                                                                   >  2:820534/1‑233 (MQ=255)

                                                            tgtAATTCGAAACGGCTGAAGCGGGCGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGAAGCAGGTTGTTGTGTTTTCTTCGGTTCCGGTTCCCCGAGCGCTTCCAGCTCCTCAAGGG‑ATTTACCTTTAGTTTCCGGGACAAA‑TTGCCACATAAAC‑AGTGCTGCCAAAACGCCCCTCAAACCGTAAATC‑CAGAAGGAAAAACCG‑TGTTGAAAAggg                                                                                                                                                                                  >  2:147793/1‑239 (MQ=255)

                                                            tgtAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATgg                                                                                                                                                                                   >  1:638505/1‑239 (MQ=255)

                                                                aTTCGAAAGGGCTGAAGCGGGAATAAAAAGCCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGcca                                                                                                                                                                               <  1:596902/239‑1 (MQ=255)

                                                                 ttCGAAACGGGTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGAAGCAGTTTGTTATGGTTTCATCGTTTCAGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTCGGTTTCCGGGGCAAA‑GTTCCACATAAAA‑CGTGCTGCCAGAACGCACATACAAACGTAACTC‑CAGCAGGGGAAAACGTTATGGAAATGGGccac                                                                                                                                                                              >  2:445102/1‑239 (MQ=255)

                                                                  tCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAACC‑AGTGCTGCCAAAACGCCCACACAACCGAAAATC‑CAGTAGGGGAAAACCGTGGGGAAAGAGGcaacc                                                                                                                                                                             >  2:288466/1‑239 (MQ=255)

                                                                      accGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACATCGTAGCAGTATGTTGTGTTTTCTTAGTTTCCGGTTCCCAGAGCGCTTCCAGCCCCTCAAGTG‑TTTTACCTTTGGTTTCCGGGACCAC‑TTTCCACCTACAC‑AGTGTTCCCACACTCCCCATACAAACTGCAATC‑CAGTAGAGGAAACCGCTCGGGAAACGGGacaccactc                                                                                                                                                                         >  2:76134/3‑236 (MQ=255)

                                                                      aaCGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCCCATAAAC‑AGTGCTGCCAGAATCCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGCGGCAATGGGcgcacagcc                                                                                                                                                                         >  1:367363/1‑231 (MQ=255)

                                                                      aaCGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCAccagcc                                                                                                                                                                         >  1:382160/1‑239 (MQ=255)

                                                                      aaCGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGATTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTCCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTGGTGGAAAGGGGCGAccagcc                                                                                                                                                                         >  1:519480/1‑239 (MQ=255)

                                                                           cTGAAGCGGGATTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAgg                                                                                                                                                                      <  1:856173/237‑1 (MQ=255)

                                                                           cTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGAAGGAGAAACCGTTGGGGAAATGGGCCACAACCCAGGAg                                                                                                                                                                    >  2:250327/1‑239 (MQ=255)

                                                                                   ggAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGATTCCAGCTCCTCAAGGG‑TTTTACCTTTGGGTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAAAC‑CATGAGACAAAACCGTTTGGGAACAGGGCACCCAGCCAGGAGttttggcg                                                                                                                                                            >  2:259370/1‑235 (MQ=255)

                                                                                         aaaaGTCAGCACGCCGAAATGGCGCGGCGTGCTTGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCcatcat                                                                                                                                                      <  1:131915/239‑1 (MQ=255)

                                                                                             tccccccccccGAATTGGCGGGGTTTGCTGGGAAGGAAGATTACGGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCggg                                                                                                                                                  <  2:390750/230‑1 (MQ=255)

                                                                                              tcacaccGCCGAAATGTGGCGGGGTTCAGGAAAGGAAGGTTTCAGCGTAGCAGGTTGTTGGGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCACCGGGa                                                                                                                                                 <  2:550062/233‑1 (MQ=255)

                                                                                               cAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGa                                                                                                                                                 <  1:144699/238‑1 (MQ=255)

                                                                                                aGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCACCAAGAAGTTTTTGGCCATAAACGGGAAg                                                                                                                                               >  2:398695/1‑239 (MQ=255)

                                                                                                 gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGGCAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCCGCCCGGCGTTTTTGTACATCATCGGaaaag                                                                                                                                              >  2:567017/1‑237 (MQ=255)

                                                                                                 gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAACC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGccac                                                                                                                                                                              <  2:861318/207‑1 (MQ=255)

                                                                                                 gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAACC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGccac                                                                                                                                                                              >  1:861318/1‑207 (MQ=255)

                                                                                                 gCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTGGTGGAAATGGGCCACCAGCCAGGAGGTTTTTTCCATCATCGGGAAgg                                                                                                                                              >  1:390750/1‑239 (MQ=255)

                                                                                                     gCCGAAATGGCGGGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCa                                                                                                                                          <  1:675588/239‑1 (MQ=255)

                                                                                                     gCCGAAAGGGCGCGGGGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGAAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCa                                                                                                                                          <  1:843955/239‑1 (MQ=255)

                                                                                                               cgcgGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGGGACGAag                                                                                                                                >  1:228640/1‑239 (MQ=255)

                                                                                                                 cggcgTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGCGTTTTTGTCCATCATCGGGAAGGTCCAGGGGACGAagaa                                                                                                                              >  1:351798/1‑237 (MQ=255)

                                                                                                                   gcgTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCAACGGGAAGGTCCAGGACACGAagaaat                                                                                                                            >  2:787334/1‑235 (MQ=255)

                                                                                                                                gAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCAAGAACGCCCATACAACCGTAAATC‑CCGTAGGAGAAACCGTTGTGGCAATGGGCCGACAGCCAGGAGTTTTTGTCCCTCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTg                                                                                                               >  1:431083/1‑239 (MQ=255)

                                                                                                                                 aaGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCAc                                                                                                                 <  1:787334/236‑1 (MQ=255)

                                                                                                                                    tttACCGCGTTGCTTTTTTTTTTTTTTTCTTCGTTTCCGTTTCCCAGAGCCTGTCCCGCTCCGCAAGGG‑TTTTCCCTTTGGTTTCCGGGACAAC‑TTCCCAAATAAAC‑AGGGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAAGGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGgc                                                                                                            <  2:412855/237‑1 (MQ=255)

                                                                                                                                     ttACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCGGCCACTGGgcc                                                                                                           >  1:327559/1‑238 (MQ=255)

                                                                                                                                        cAGCGTAGCATTTTTTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCATCTCCCAAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTGCCACATAACC‑AGTGCTGCCAGAACGCCCATCCAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCa                                                                                                       <  1:429995/239‑1 (MQ=255)

                                                                                                                                        cAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCa                                                                                                       <  1:609790/239‑1 (MQ=255)

                                                                                                                                           cGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGACGTTCGCCAGCCCCTGGGCCGCCAcc                                                                                                     >  2:559387/1‑238 (MQ=255)

                                                                                                                                           cGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGATCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAGA‑TTTCCACATAAAC‑AGTGCTGCCAGAACTCCCATACAACCGTAAATC‑CCGTAGGCGACACCGTTGTGGAAATGAGCCACCAGCCAGGAGTTTTTGTCCATCACCGGGAAGGTCCAAGTGACGAAGTAGTTAGCCAGCCAACGGGCCGACACcg                                                                                                    >  1:323601/1‑239 (MQ=255)

                                                                                                                                             tAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCACCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAAACGAAGGAGTTCGCCAGCCACTGGGCCGCccccc                                                                                                    >  2:306300/1‑236 (MQ=255)

                                                                                                                                             tAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGGTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGCGGAAATGGGCCACCGCCCAGGAGTTTTTGTCCATACTCCGGAAGGTCCAGGGAACGAAGAAGTTCGCCCTCCACTGGGCGGCAACggcg                                                                                                  >  2:748493/1‑239 (MQ=255)

                                                                                                                                              aGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACACCCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCAACCAGCCAGGCGATTTTGTCCATCATCGGGAAGGCCCGGGCAACAAAGAAGGTGGACCCCCACAGGCCCGCCAcggaga                                                                                                 >  2:388678/1‑234 (MQ=255)

                                                                                                                                              aGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGa                                                                                                 >  1:301481/1‑239 (MQ=255)

                                                                                                                                                         tttttttCTTCGGTTCCGGTTCCCAGAGCGCTTCCAGCCCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGGGCTGCCAGAACGCCCATACAGCCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCt                                                                                      <  1:291145/236‑1 (MQ=255)

                                                                                                                                                            ttttCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAACC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAACAGATCGCAAGACACTGGGCCGCCCCCGCGATTGACAGGGCttt                                                                                    >  2:697639/1‑238 (MQ=255)

                                                                                                                                                              ttctgcGTTTCCGGTTCCCAGCGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTAcc                                                                                 <  1:304724/239‑1 (MQ=255)

                                                                                                                                                               tcttcGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTAcc                                                                                 >  1:736209/1‑238 (MQ=255)

                                                                                                                                                                 ttcGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCCCCGCGATTGCCAGCGCTTTACCACg                                                                              >  1:509877/1‑239 (MQ=255)

                                                                                                                                                                          ggTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTTCCTTTGGTTTCCGGGACAAA‑TTCCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCAtt                                                                     <  1:6908/239‑1 (MQ=255)

                                                                                                                                                                               ccAGAGCTTTTCCAGCTCCTCAAGGG‑TTTGTCCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCAAACAACCTTAAATC‑CAGTAGGAAAACCCGTTGTGGAAATGGGCCACCAGCAAGGATTTTTTGTCCATCATGGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGa                                                                <  2:879759/239‑1 (MQ=255)

                                                                                                                                                                                         tcccactcctcAAGGG‑TTTTACCTTTGGTTTACGGGACAAA‑TTTCCAAATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGa                                                      <  1:802864/234‑1 (MQ=255)

                                                                                                                                                                                            acccgcctaaGGGTTTTTTCCTTTTGTTTCCGGGAAAAA‑TTCCCAAATAAAC‑ATGGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTGGGAGAAACCGTTTGGGAAATGGGCCACCAGCCGGGAGTTTTGTCCCATCATCGGGAAGGTCCAGGAGACGAATTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGa                                                      <  2:140204/229‑1 (MQ=255)

                                                                                                                                                                                             gCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATGCAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGCCCCGGAGCCGAAGCAGTTCGCCAGCAACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAAAAGCATTCGGAAAAAATTcccaaaga                                                  >  2:322780/1‑233 (MQ=255)

                                                                                                                                                                                              ctcctcGAGGT‑TTTTATTTTGTGTTTCGCAGACAAT‑TTCCCACTAAAAC‑GGGGCTCAGAAAACGCCCATAAACCCGTAAACG‑TAGTAGGAAACACCGGTGTGGAAACGGGCCTCCAGCGAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagca                                                 <  2:430215/239‑1 (MQ=255)

                                                                                                                                                                                              ctcctcAAGGG‑TTTTACCTTTGGTTTCCGGGAAAAA‑TTTCCACATAAAC‑AGGGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcagca                                                 <  1:224979/239‑1 (MQ=255)

                                                                                                                                                                                               tcctcAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCAAATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATACGGGAAGATTTCCGAcagca                                                 <  1:398695/238‑1 (MQ=255)

                                                                                                                                                                                               tcctaAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACCCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcag                                                   <  1:579087/236‑1 (MQ=255)

                                                                                                                                                                                                 ctcAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACACCAGt                                               >  1:738216/1‑238 (MQ=255)

                                                                                                                                                                                                 ctcAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCCCGATTGCCAGGCCTTTACCCCGAATAGCATTCGGGAAGATTTCCGAccgcaga                                               >  2:274266/1‑237 (MQ=255)

                                                                                                                                                                                                  tcAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTa                                              >  1:164771/1‑238 (MQ=255)

                                                                                                                                                                                                   tagggg‑TTTTTCTTTTGTTTTCAGGG‑CAAA‑ATTCCACAAAAACAAGGGCTCCCAGAACCCCCAAACAACCGTAAATC‑AAGGGGGAGAACCCGTTGGGGAAAGGGGCCACCAGCCAGGAGTTTTGGCCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAcc                                            <  2:549680/236‑1 (MQ=255)

                                                                                                                                                                                                    catgg‑TTTTTTCTTTGGTTTTCGGGGCAAATTTTCCAAAAAAAA‑AGGGCTGCACAACCGCCCATAACACCGAAAACC‑AAGTGGGAAAACCCGTTGGGGAATGGGGCCACCAGCCGGGATTTTTTGCCCACCACCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAcc                                            <  2:738189/236‑1 (MQ=255)

                                                                                                                                                                                                           tttACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGCTTTTGTCCATCATCGGGAAGGTCCAAGAGACGAAGTAGTTCGCCAGCCCCTGGGCCGCCACCGCGATTGCCAGCGCTATACCACGCATAGAATTCGGGAAGAGTTCCGACCGCAGTACCCAGcct                                      >  1:706186/1‑236 (MQ=255)

                                                                                                                                                                                                            ttcccTTTGGTTTCCGGGACAAA‑TTTCCAAATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAc                                    <  1:748493/236‑1 (MQ=255)

                                                                                                                                                                                                            ttACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGCGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAAACGAAGTAGTTCGCCAGCCCCTGGTCCGCCACCGCGCTTGCCAGCGCTTTACCCCGCATAGCATTCGGGAAGATTTCCGACACCAGGACCCCCCATAc                                    >  1:395503/1‑239 (MQ=255)

                                                                                                                                                                                                            ttACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGCTTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGCTTTCCGACCGCAGTACCCCGCCTa                                     >  1:741205/1‑238 (MQ=255)

                                                                                                                                                                                                            ttACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAcc                                            >  1:140204/1‑231 (MQ=255)

                                                                                                                                                                                                            ttACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGCAGTTCGCCAGCCACTGGGCCGCCACCGCGATTCCCAGCGCTTTACTACGAAAGCCATTCGGGAAGATTTCCGCAAGCGGAACCCACCAAAc                                    >  2:289249/1‑239 (MQ=255)

                                                                                                                                                                                                             tACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGTAATGGGCCACCAGCCAGGAGTTTTTGTCCATCACCGGGAAGGTCCAGGTGCCGCAGCAGTTCGCCAGCCCCTGGGCCGCCACCGCGATTTCAAGCGCTTTACCACGAATTGCATTCGGGACGATTTCCGACAGCAGCACCAAGCaccac                                   >  2:686622/1‑235 (MQ=255)

                                                                                                                                                                                                             tACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCCGTACCCAGCATAcc                                   >  1:86226/1‑239 (MQ=255)

                                                                                                                                                                                                              tccTTTGGGTCACGGGAAAAA‑TTCCCACAAAACC‑GGGTCTGCCAAACCGCCAATACAACCGTAAATC‑CGGTAGGAAAATCCGTTGGGGAAATGGGCCACCACCCAGGATTTTTTGTCCTTCACCGGGAAGGTCCAGGAGAGAAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                  <  2:638505/238‑1 (MQ=255)

                                                                                                                                                                                                              cccTTTGGTTGCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATCCACCCGTAAATC‑CAGTAGGAGAAACCGTTGGGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                  <  1:19640/238‑1 (MQ=255)

                                                                                                                                                                                                              cccTGTGGTTCCCGGGAAAAA‑TTCCCAAAAAAAC‑AGGCCTGCCAGACCCCCAATCAACCCGTAAAGC‑CAGGAGGAGAAACCGTGGTGGAAATGGGCCCCCAGCCAGGAGTTTTGGCCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                  <  1:9010/238‑1 (MQ=255)

                                                                                                                                                                                                              aCCTTTGGTTTCCGGTAAAAA‑TTCCCCCATAACC‑ATTGCGTCCAGAACCCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCAGCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                  <  1:776487/239‑1 (MQ=255)

                                                                                                                                                                                                              aCCTTTGGTTCCCGGGACAAA‑TTTCCACATAAAC‑AGGGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGGGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCg                                  <  1:567017/239‑1 (MQ=255)

                                                                                                                                                                                                                  ttGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGCAGTTCGCCAGCCACTGGTCCGCCACCGCGATTGCCCGCGCTTTACCACGAATAGCATTCGGGACGATTTCCGACCACCGTACCCAGCATAcgcgac                               >  1:353786/1‑233 (MQ=255)

                                                                                                                                                                                                                                       cccaaaaaaC‑TGGGCTGCCAAACCGCCCATACACCCGAAAACCACAG‑AGGAGAAACCTGTGGGGAAAGGGGCCACCCGCCAGGAGTTGTTGCCCATCAGCGGGAAGGGCAGGGAGACGAAGTAGTTCCCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcgg          <  2:227475/233‑1 (MQ=255)

                                                                                                                                                                                                                                             aaaC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGACTAGCATTCGGGAAGATTTCCGACAGCAGTACCCACCATACCGGACCCACGGAAAGGGCAAAGAGGGCAACaa    >  2:741902/1‑238 (MQ=255)

                                                                                                                                                                                                                                               aC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATAATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGCTTGCCAACGATTTCCCACGAAAAGCATTCGGGACGATTTCCGACAAAACTGCCCACCATACCGGACCCCAGGGCATGGCAAAAGCAGCAAAATAg  >  2:716045/1‑239 (MQ=255)

CCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACTGGTGATCACCCTGTGGCTGTGTAATTCGAAACGGCTGAAGCGGGAGTAAAAAGTCAGCACGCCGAAATGGCGCGGCGTGCTGGACAGGAAGATTACAGCGTAGCAGTTTGTTGTGTTTTCTTCGTTTCCGGTTCCCAGAGCGCTTCCAGCTCCTCAAGGG‑TTTTACCTTTGGTTTCCGGGACAAA‑TTTCCACATAAAC‑AGTGCTGCCAGAACGCCCATACAACCGTAAATC‑CAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAG  >  NC_000913/4240646‑4241121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`