BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,829	T→A	12.9%	L142F (TTA→TTT)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,829	0	T	A	12.9%	243.7 / 6.7	106	L142F (TTA→TTT)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (66/23); new base A (6/10); total (73/33)
Fisher's exact test for biased strand distribution p-value = 6.93e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACC  >  NC_000913/4241591‑4242056

cTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCt                                                                                                                                                                                                                                     <  1:72443/239‑1 (MQ=255)

      cggtagaacgcaccaTTAAGAGCAGGAGTGCGTGAATGCAGTCCAAGGCAAACATATAACGCCAGCCTTCAGTATTGAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAATCTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGTCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAAcc                                                                                                                                                                                                                                <  1:93377/229‑1 (MQ=255)

      aggTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGAAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAAcc                                                                                                                                                                                                                                <  1:399191/237‑1 (MQ=255)

       ggtgtaaaacagcaTTAAGAACTGCAGTTCAGGGAGACATTCCGACGCAAACAGATAACGACAACCGTCAGTATGCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTGCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCaa                                                                                                                                                                                                                              <  2:156981/230‑1 (MQ=255)

                   caTTTAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGGTGCCCGAAAATAATCCCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGCATATGAGCTGGAGCCATATCCGC‑AATAAACATTGGC‑GAGAGC‑ATTGAGGCTACACCAACCCCAATAcca                                                                                                                                                                                                                   >  1:601170/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGACAATAATCACAAAC‑TGGATAAAAGA‑GACCAGTTTCCCCCGACTATGAACTGGAGCCAGTTCCTC‑AATATCCATTGAC‑GCGAGC‑ATTGAAGCTAAACCAACGCCacaaacg                                                                                                                                                                                                                   >  2:644060/1‑232 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GCCCAGTTTCCCGCGAATATGAGCTGGGGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTAGGGCTAAACCAAAGCCAAAAcccc                                                                                                                                                                                                                  >  2:198486/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCTAGAATATGAGCTGGAGCCAGTTCCGC‑ACATTACATTGGC‑GGGAAC‑ATTAAGGCTAAACCAAGGCCataaacgc                                                                                                                                                                                                                  >  1:732926/1‑232 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGTC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCg                                                                                                                                                                                                                   >  1:418535/1‑238 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCCATACCGc                                                                                                                                                                                                                  >  1:768521/1‑239 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCg                                                                                                                                                                                                                   >  1:758076/1‑238 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGc                                                                                                                                                                                                                  >  1:838294/1‑239 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGA‑ATTTAGGCTAAACAAACGCaaaaa                                                                                                                                                                                                                      >  2:399191/1‑233 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGCC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCg                                                                                                                                                                                                                   >  1:734355/1‑238 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATAAACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAAAAcccc                                                                                                                                                                                                                  >  1:395732/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAACTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAAAGCCAAAACCGc                                                                                                                                                                                                                  >  2:232970/1‑239 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATAAGCTGGAGCCAGTTCCGC‑AATATCAATTGGC‑GAGAGA‑ATTGAGGCTAAACCAACCCCAATAcccc                                                                                                                                                                                                                  >  2:773705/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAAAATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAAAGC‑ATTGAGGCTAAACCAATGCCAATACCg                                                                                                                                                                                                                   >  1:646461/1‑238 (MQ=255)

                           aCAGCAGTGCAGGAATCCATTCCGAGGCAAACATAAACCCCCAGCCGTCATTTTTCAGCCAGCTGGAATCACCGGAACGGCCAATAAAATAGTTTCCGAAGTAAACTAAAGGTTGCCCAAAAATAATCGAAAAC‑GGGTTAAAAGA‑GACCATTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGA‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATg                                                                                                                                                                                                          <  1:582106/239‑1 (MQ=255)

                             agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCAGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAAT‑GACCAGTTTCCTGCGAATATGAGCTGGCGCCAGTCTCCC‑ATTAAACCTTGGC‑GAGAGC‑ATTTGGGCATAACAAAGTCTCATACCTCCAATAATCCg                                                                                                                                                                                                        >  1:204587/1‑239 (MQ=255)

                             agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGACATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAACTAATCGCAACA‑TGGTTAAAAGA‑GACCAGTTTCACCCGAATATAAGCTGAGGCCACTTCCGC‑AAGATCCATTGGC‑GAGAGC‑ATTAAGACTAAACCAACGCCACTACAGCCaacaatccc                                                                                                                                                                                                        >  1:290001/1‑236 (MQ=255)

                               cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCATCCAGATGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAACCTAAAAGTTACACGACAATAATCTCAACA‑CGGCTAAAGGA‑GACCAGTTTCCCTCGAGTATCACCTGCAGCCAGTTCCCC‑CACAAACATTGGC‑TAGAGG‑ATTGAGACAAAACCAAACCCAAAAACCCCaaaaaagggga                                                                                                                                                                                                      >  2:427897/1‑233 (MQ=255)

                               cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GCGAGA‑ATTTAGGCTAAACCAACGCCAATACCGCCAATAATGCGat                                                                                                                                                                                                      >  1:696981/1‑239 (MQ=255)

                               cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATCCGat                                                                                                                                                                                                      >  1:619843/1‑239 (MQ=255)

                                  tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGTC‑GAGGGA‑ATTGAGGCAAAACCAACGCCAATACCGCCAAAAATGCGaaaaa                                                                                                                                                                                                   >  2:727792/1‑239 (MQ=255)

                                  tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTAGC‑GAGAGC‑ATTAAGGCAAAACCAACGCCAAAAACCCCAAAAAACCGaaaaa                                                                                                                                                                                                   >  2:844883/1‑239 (MQ=255)

                                  tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGACGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCACGAAAATAATCGCAAAC‑TGCTTAAAAGA‑GACCAGATTACCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATAAACATTGCC‑GAGAGC‑ATTCAGGCTAAAACAACGCCCATACCGACAAAAAAGCGaaaaa                                                                                                                                                                                                   >  2:240882/1‑239 (MQ=255)

                                    cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCCGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAACA‑GACCAGTTTCCCGCGCATATGAGCTGGAGCCAGGTCCGC‑AATATACATTGGC‑GGGAGC‑ATTTAGGCTAAACCAACGCCAAAACCCCCaataaaacaacaaaaa                                                                                                                                                                                                 >  2:111927/1‑228 (MQ=255)

                                         aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACaaa                                                                                                                                                                                            >  1:209374/1‑239 (MQ=255)

                                         aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCACAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCAAAACCAACGCCAATACCCCCAAAAAAGCGataaaacaaaaa                                                                                                                                                                                            >  2:72155/1‑232 (MQ=255)

                                            cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAAtt                                                                                                                                                                                          >  1:662029/1‑238 (MQ=255)

                                            cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGCC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAAtt                                                                                                                                                                                          >  1:798694/1‑238 (MQ=255)

                                            cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCCCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAAAGCCAATACCGCCAAAAAAGCGATAAAAAAAAAAATc                                                                                                                                                                                         >  2:258426/1‑239 (MQ=255)

                                             ttcccGAGGAAAACATAAACCCCCAGCCGTCAGTATTCAGCAAGCGGGAAACACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTGGCCCGAAAATAATCGAAACC‑TGGTTAAAAGA‑GACCAGTTCCCCGCAAATATGAGCTGGAGCCAGTCCCGC‑AATATACATTGGA‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                                                                        <  1:315261/236‑1 (MQ=255)

                                             aTTCCGAGTAAAACATAAAACGCCAGCCGACAGTATTCAGCCAGCTGCAATCACCGGAACGGGAAATAAAATAGTTTACGCAGTAAACTAAAAGTAGCCCGAAAAAAATCGCAAAC‑TGGTTAAAAGA‑GACCATTTCCCCGCAAATAGGAGCTGGAGCCAGTCCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                                                                        <  1:152723/239‑1 (MQ=255)

                                                   aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGCATATGAGCTGGAGCCAGTTACGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAAAGCCAATACCCCCAATAATTCGTAAAATAAAAAATTccagaaac                                                                                                                                                                                  >  2:890860/1‑233 (MQ=255)

                                                   aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCAGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCACCGCCAATACCGCCAATAATTCGATAACTACCAAATTCCGGGACa                                                                                                                                                                                  >  1:827301/1‑239 (MQ=255)

                                                   aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATAAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAAC‑ATTGAGGCTAAACCAACGCCAAAACCGCCAAAAAAGGCAAAAAAAAAAAAATCCGGGaaa                                                                                                                                                                                  >  2:753456/1‑237 (MQ=255)

                                                    ggCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACAt                                                                                                                                                                                 >  1:447886/1‑239 (MQ=255)

                                                               aggcccACCCGTCAGTTACCAGCCAGCTGGAATCACCGGAACGGGCAAAAAAATAGTTTACCCAATAAACTAAAAGTTGCCCGAAAATAATCCAAAAT‑GGGTAAAAAGA‑GACCAGTTCCCCGCGAATAGGAGCTGGACCCAGTACCGC‑AATATACATGGGC‑GAGAGC‑ATTGAGGAAAAACAACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                                                                      <  1:390972/235‑1 (MQ=255)

                                                               aaCGCCGGCCGTCATTTTTCAGCCAGGTGGCAACCCCGGACCGGGAAAAAAAATAGTTTACGAAGTAACCTAAAATTTCCCCGAAAATAATCGAAAAC‑TGGTTAAAAGA‑GACCGGTTCCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                                                                      <  1:32046/239‑1 (MQ=255)

                                                               aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGTC‑GAGAGC‑ATTGAGCCTAAACCAACGCCAATACCGCCAATAATGCGATAAATACCAAAATCCGGGACAAAACCCGCCAGa                                                                                                                                                                      >  2:692242/1‑239 (MQ=255)

                                                               aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGGGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTACGGGACATAACCTGCCAGa                                                                                                                                                                      >  1:217729/1‑239 (MQ=255)

                                                                     aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACa                                                                                                                                                                >  1:677796/1‑239 (MQ=255)

                                                                     aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCAGGGACATAACCTGCCAGATAAACa                                                                                                                                                                >  1:582628/1‑239 (MQ=255)

                                                                      gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AAAATACATTGGC‑GAGAGC‑ATTGAGGCCAAACCAACGCCAATACCCCCAATAATGCGAAAAAAAACAAATTCCCGGACATAACCTGCCAAAGaaaacg                                                                                                                                                               >  2:242988/1‑236 (MQ=255)

                                                                       ccGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAAAAAATTCCGGAAAAAAACAGGCCAAAAAAAAAgg                                                                                                                                                              >  2:431892/1‑239 (MQ=255)

                                                                        cGTCAGTATTCAGCCAGCGGGCATACCGGGAACGGGAAATAAAATAGTTTCCGAAGTAAACAAAAAGTTGCCAAAAAAAAATCGCAAAC‑GGGTTAAAAGA‑GACCATTTTCCGGGGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATGGGA‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGc                                                                                                                                                             <  1:538632/239‑1 (MQ=255)

                                                                           cAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGCCATAACCTGCCGGTTAAACAGGccca                                                                                                                                                          >  1:784841/1‑239 (MQ=255)

                                                                            attATCCAGACAGCTGGCATCCGCGGAGCGGGAAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCAAAAAGAATGGCAAGC‑TGGTTAAAAGA‑GACCGGTTTCCGGGGAATATGAGCTGGAGCCAGTTCCGC‑AATATCCATTGGG‑GAGAGC‑ATTGAGGATAAACCAGCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAg                                                                                                                                                         <  1:77381/237‑1 (MQ=255)

                                                                                   agccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCCCAGagttgt                                                                                                                                                   >  1:305764/1‑238 (MQ=255)

                                                                                      cagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGAAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTCCCCGCGAATATGAGCTGGAGCCAGTCCCGA‑AATATACATTGGC‑GAGAGC‑ATGGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCgg                                                                                                                                               <  1:477527/239‑1 (MQ=255)

                                                                                             cATCACAGGAAAGGGCAAAAAAATTGTTTACGCAGTAAACAAAATGTAGCCCGAAAATAACCGCAAAC‑GGGTAAAAAGA‑GACAAGTTACCCGCGAATAGGAGCTGGAGCCAGTCCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCAAAACCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTtat                                                                                                                                         <  1:890860/238‑1 (MQ=255)

                                                                                                   cGGAACGGTCAATAAAAAAGTTAACGAATTAAATAAAAAGTTGCCGAAAAATAA‑CGCAAACTTGGTAAAAGGA‑GACCAGTTCCCCGGGAAAAAGAGCGGGAGCAAGTTCCGA‑AATATACTTGGGC‑GAGAGC‑ATTGAGGTTAAACCATCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTa                                                                                                                                  <  1:242988/239‑1 (MQ=255)

                                                                                                   cGGAACGGGCAATAAAATGGTTTAGGAAGAAAACTAAAAGTGGCCGAAAAATAAACGAAAAC‑TGGTTAAAAGG‑AACCAGTGCCCGGCGAATATGAGCTGGAGCCAGTCCCGC‑AATATACATGGGC‑GAGAGC‑ATTGAGGCAAACCCAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTa                                                                                                                                  <  1:896413/239‑1 (MQ=255)

                                                                                                          ggCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAAAGGAACAGTGTTTTCCGGGGTTAAAAAGGACAAACCaa                                                                                                                           >  2:183910/1‑239 (MQ=255)

                                                                                                                        ttACGCAAAAAAAAAAAAGTTCCCAGAAAATAAGCGAAAAG‑GTGTAAAAAGA‑CACCATGTTCCCGCGAATATGAGCTGGACCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGATAAACCAACGCCAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                                             <  2:645694/239‑1 (MQ=255)

                                                                                                                         tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCAg                                                                                                            >  1:195718/1‑239 (MQ=255)

                                                                                                                            gCAGTAAACTAAAAGTTGCCAAAAAATAATCGAAAAC‑GGGTTAAAAGA‑GACCAGTGTCCCGCGAATATGAGCTGGAGCCAGTTCCAC‑AATATACATTGGC‑GAGAGA‑ATTGAGGCTAAACCACCGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAAc                                                                                                         <  1:844883/239‑1 (MQ=255)

                                                                                                                              aGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGTTTAAAAGA‑GACAAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGCCTAAACCAACGCCAATACCGCCAATAATGCGATAAATCACAAATTCCGGGGCATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGCTCTGGCCCGGCAAAACCt                                                                                                       >  1:802011/1‑239 (MQ=255)

                                                                                                                                tAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGCCATAACCTGCCAGATAACCAGGCCCCGTGTTGTCCGGGTTTATTAAGGCAAAACCAAGTTCTGGCCAgcacgaaccca                                                                                                      >  2:520829/1‑228 (MQ=255)

                                                                                                                                tAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGAAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGAAAAACCAAGTACAGGCAAGGCAAAAccaaa                                                                                                     >  2:325460/1‑236 (MQ=255)

                                                                                                                                            tcaccaaaaaaaATCCC‑AAC‑TGGTTAAAAAAGGACCAGTTCCCCGAAAATAGGGCCGGGAGCCATTCCCGA‑AATAACCATGGGC‑GAGAGC‑ATAGAGGCTAACCCAACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                                                                           <  2:85585/231‑1 (MQ=255)

                                                                                                                                              cccGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGGGCCAGTTCCGC‑AATATACATTGGC‑GAGCGC‑ATTGAGGCTAAACCAACGCCCATACCGCCAATAATGCGATAAATAACACATTCCGGGCCATAACCTGCCAGATAAACAGGCACAGTGTTGTCCAGGTTTATAGAGGTAAAACCAAATGCCGACCAGGCCGAACCTCCCCCATAaaaaaaaa                                                                                       >  2:774238/1‑239 (MQ=255)

                                                                                                                                                     aatatccccaaC‑TGTTTAAAAGA‑CCCTGGTTCCCCGGAAATGGGAGCTGGAACCATTTCCGA‑AATATAAATTGGC‑GAGAAC‑TTTGGGGATAAACAAAAGTCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAc                                                                                <  2:696981/230‑1 (MQ=255)

                                                                                                                                                          ccaaaaC‑TGGTAAAAAAG‑GCCCATTTCCCCGAGAAAAGGGGCTGGAGCCAGTTCCGA‑AATATACATTGGG‑GAAAGC‑ATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagcag                                                                           <  1:431892/236‑1 (MQ=255)

                                                                                                                                                             caacgtGGTAAAAAGA‑GACCAGTTCCCCGAGAATAGGAGCTGGACCCAGTCCCGA‑AATATAAATGGGC‑GAGAGC‑ATTGAGCCAAAACCACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAgcagca                                                                         <  1:621661/234‑1 (MQ=255)

                                                                                                                                                              aaC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCaa                                                                        >  1:141781/1‑238 (MQ=255)

                                                                                                                                                               actgggTTACAAGA‑CACCGTTTTCCCGGGAATTTGGGCGTGAGCGAGTCCCCC‑AATAT‑CCTTGGCAGAGAGA‑TGCGAGGATAAACAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAGCCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACAACAGAAATAAAAAACAGGACAGCAGCAAt                                                                       <  2:571007/235‑1 (MQ=255)

                                                                                                                                                                     ttAAAAGG‑ACCCAGTTCCCCGAGAATATGAGCGGGACCCAGTCCCGC‑AATATACGTTGGA‑GAGAGC‑ATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAg                                                                 <  1:852040/239‑1 (MQ=255)

                                                                                                                                                                     taaaaaaa‑aaCCAGTTCCCGGGAAATGTGGGCGGGAGCAATCTCCCA‑AATATCAATGGGA‑GAGAGC‑ATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                                  <  2:447886/236‑1 (MQ=255)

                                                                                                                                                                               aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacg                                                        >  1:386308/1‑239 (MQ=255)

                                                                                                                                                                               aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGGAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAAAAAAAAAAAGGAAAACAGAAAATTTaaaggaaaaacc                                                        >  2:465545/1‑229 (MQ=255)

                                                                                                                                                                                    tttCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCAcgacga                                                    >  1:104182/1‑238 (MQ=255)

                                                                                                                                                                                    tttCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTAACCCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTaaaaaacaagaa                                                    >  2:326492/1‑229 (MQ=255)

                                                                                                                                                                                       cccGGGAATATGACCGGGACCCAGTCCCAC‑AATATACATTGGC‑GAGAGC‑ATGGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGa                                                <  1:192809/239‑1 (MQ=255)

                                                                                                                                                                                               tatGAGTGGGAGCCAGTCCCGA‑AATAGCATTTGGC‑GAAGGC‑ATTGAGGCTAACCCACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                        <  1:509040/239‑1 (MQ=255)

                                                                                                                                                                                               attgggCTGGA‑CCCGTTCCCCAAATATACATTGGC‑‑AGAGCAATTGAGGTAAAACCAACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                        <  1:483693/234‑1 (MQ=255)

                                                                                                                                                                                                atGAGCTGGAGCCAGGTCCGC‑AATATCCATTGGC‑GAGGGC‑ATGGAGGCTAAACCAACGCAAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTAc                                       <  1:72155/239‑1 (MQ=255)

                                                                                                                                                                                                    gtgggAGCCAGTTCCAC‑AATATACATTGGC‑GAGAGC‑ATTGAGCAAAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGc                                    <  1:258426/235‑1 (MQ=255)

                                                                                                                                                                                                      ggggcccAGTCCCGA‑AATATACTTGGGC‑AAGAGA‑TTTAAGGAAAACCAAATGCCAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAAt                                 <  2:646461/234‑1 (MQ=255)

                                                                                                                                                                                                        gAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                >  1:859003/1‑238 (MQ=255)

                                                                                                                                                                                                        gAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                >  1:846145/1‑238 (MQ=255)

                                                                                                                                                                                                         aGCCAGTTCCTA‑AATATACAGTGGC‑GAGAGC‑ATTGAGGCTAAACAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                               <  1:520829/238‑1 (MQ=255)

                                                                                                                                                                                                          gCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAACCGGTTCCTGCAATAacc                             >  1:171876/1‑239 (MQ=255)

                                                                                                                                                                                                                             aTTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAcccccggt           >  1:374639/1‑237 (MQ=255)

                                                                                                                                                                                                                             aTTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGAACCGCCgat           >  1:23256/1‑239 (MQ=255)

                                                                                                                                                                                                                               tGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga         >  1:24338/1‑239 (MQ=255)

                                                                                                                                                                                                                               tGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGACCCGCCgatga         >  1:771725/1‑239 (MQ=255)

                                                                                                                                                                                                                               tGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAACGGGTTACTCCAAAAACCAACCAGGGCaccacccaaga         >  2:207445/1‑234 (MQ=255)

                                                                                                                                                                                                                                  c‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCCAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGc      >  1:394927/1‑239 (MQ=255)

                                                                                                                                                                                                                                  c‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTTACTGCAATAACAACCGAGGGCACCCCCgatgat        >  1:388584/1‑237 (MQ=255)

                                                                                                                                                                                                                                  c‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCCACACCAGAAACAAAAAACCGAACAGCAGCAATCTTACGCGAATCACGACGACCGAAGCGCTACCTGCAAAAAAAACCCAGGGAACAGCCgataaaca      >  2:363670/1‑234 (MQ=255)

                                                                                                                                                                                                                                     agagC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGTGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                >  1:867945/1‑211 (MQ=255)

                                                                                                                                                                                                                                     agagC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAAGCGGTTACTGCAATAACCACCCAGGGCACCGCCGAATATGCa     >  1:779998/1‑238 (MQ=255)

                                                                                                                                                                                                                                     agagC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGAGGATGCa     >  1:30926/1‑238 (MQ=255)

                                                                                                                                                                                                                                     agagC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGACCCCCCGATGATGaaa    >  2:775138/1‑239 (MQ=255)

                                                                                                                                                                                                                                     agagC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAACTCCGGGACATAACCTACCAGATACACAGGAACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGACATAAAAAACAGGACAGCAGCAATCTTAAGAGAATCACGACGACAGAAGCGGTTACTGCAATAACAACCCAAGGCACCGCCGATAAGGCa     >  1:274850/1‑238 (MQ=255)

                                                                                                                                                                                                                                       agC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAACTAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAAGGCACCGCCGATGATGCAAcc  >  1:337047/1‑239 (MQ=255)

                                                                                                                                                                                                                                       agC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACACCGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGTGTATGCAAcc  >  1:706940/1‑239 (MQ=255)

CTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAAC‑TGGTTAAAAGA‑GACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGC‑AATATACATTGGC‑GAGAGC‑ATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACC  >  NC_000913/4241591‑4242056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`