Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,280,577 A→G 22.9% S200G (AGC→GGC)  yjcE → putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,280,5770AG22.9% 99.6 / 3.7 54S200G (AGC→GGC) yjcEputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (5/35);  new base G (11/3);  total (16/38)
Fisher's exact test for biased strand distribution p-value = 1.18e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTGGGCGATGCTGGA  >  NC_000913/4280342‑4280815
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                
gttGCTTTCTCCGACCGATTCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAACAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAgct                                                                                                                                                                                                                                               <  1:393502/237‑1 (MQ=255)
ggTGCTTTCGCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAgc                                                                                                                                                                                                                                                <  1:693615/238‑1 (MQ=255)
     tttCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGGATTCTCCCACGTGTTTGGGTGGgcgggctg                                                                                                                                                                                                                                          >  1:800047/1‑239 (MQ=255)
       tctcCGGACGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTa                                                                                                                                                                                                                                        <  1:226188/239‑1 (MQ=255)
            gaccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCTCCGGGTTTTTGGGTGTCTGGCTTTACGGt                                                                                                                                                                                                                                    >  1:628466/1‑238 (MQ=255)
               cgaTGCTTGTGCGTTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGtcgct                                                                                                                                                                                                                                <  1:139178/239‑1 (MQ=255)
                            ctctCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGGATTTCTGCCGGGTTTTTGGGTGGCTGGCTGTACGGTGCCTCGCTGccgtttc                                                                                                                                                                                                                    >  1:371335/1‑232 (MQ=255)
                                    gATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGc                                                                                                                                                                                                            <  1:73360/238‑1 (MQ=255)
                                       tgttgGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGCCGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTCTGAAAGTGCCCATTGGCGGGATTTTTGCCCGGTTTGTGGGTGGCTTGCTGTACGCTCCGCCGCGGCGACTCTTCCGccccctgg                                                                                                                                                                                                        >  2:164146/1‑233 (MQ=255)
                                        tttgggtaaGGGGGCATCCCTAAAACAATTGTGGGCATTTTGCAGGGCGAAGGTTGTATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGGTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTgggg                                                                                                                                                                                                       <  2:243830/232‑1 (MQ=255)
                                         ttgGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGCATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTTTGGTGGGATGGCTGTACGGTCGCTCGCTGCGCTTCCTCAGCCGCTGGGGc                                                                                                                                                                                                      >  1:97883/1‑239 (MQ=255)
                                           ggCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGGATTTTCCCCGGGTTTTTGGTGGGCTGGCTTTCAGGTCGCCCCCTCCGATTCTCCAGCCCCTCgggggg                                                                                                                                                                                                    >  1:78986/1‑239 (MQ=255)
                                              gAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGGCGAATTTATGAAAGTAGCCATTGGCGGCATTCTCGGCGGTTTTGTTGTGGGCTGGCGGTTAGGGCGCTCGCTTCGGTTCCCCAGCCGCTGGGGCgggtg                                                                                                                                                                                                 >  1:12707/1‑236 (MQ=255)
                                                 gggCGCATCCCGAAAAAAATCATGGGCATTTGGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTTGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGtgatg                                                                                                                                                                                               <  1:765366/238‑1 (MQ=255)
                                                      cATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCCCCGGCTTTTTGGGGAGCGGGCTGTACGGTCGCTCCCTCCGCTTTCTCAGCCGCTGGGGCGGGGGTGAAaccc                                                                                                                                                                                         >  1:620206/1‑238 (MQ=255)
                                                      cATCCCGAAAAAAATCAGGGGCTTTGGGGAGGGGGAAGGGTTGAAGAATGACGCCTCCGGTCTGGTTTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTGCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCcg                                                                                                                                                                                         <  2:751466/239‑1 (MQ=255)
                                                             aaaaaaaTCATGGGCATTTTGCAGGGCGAAGGGTTGATGAATGACGCCTCCGGCCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGTGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCa                                                                                                                                                                                 <  1:198106/239‑1 (MQ=255)
                                                                    tCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTc                                                                                                                                                                          <  1:617881/239‑1 (MQ=255)
                                                                     tgtGGGTATTTTGGGGGGCGAAGGGTTGGAGAATGCGGCCTCCGGTCTGGTGTCGTTGAAGGTTGCGGGGCCAGTGGCGATGGGGACGATGATCTTCACCGTCGGGGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTcc                                                                                                                                                                         <  2:619487/237‑1 (MQ=255)
                                                                              tttGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCCCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACCCAGATCGTCCTGCTGtt                                                                                                                                                                  >  1:440033/1‑237 (MQ=255)
                                                                                         gagggTTGGTTAATGCCGCCCTCGGTCGGGTTTTGTTGAAGTTTGCCGTAGCAGGGGGCATGGGGAAGATGATCTTCACCGTCGGCGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTc                                                                                                                                                    <  2:789549/235‑1 (MQ=255)
                                                                                         aaGCGTTGATGAATGACGCGTCCGGGTTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCAtt                                                                                                                                                     <  1:872127/238‑1 (MQ=255)
                                                                                                       tggcGCCTCCGGTTTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGa                                                                                                                                       <  1:682249/236‑1 (MQ=255)
                                                                                                       tggcGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGa                                                                                                                                       <  1:218473/236‑1 (MQ=255)
                                                                                                       tgaCGCCTCCGGTCTGGTTTCGTTGAAGTTGGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGa                                                                                                                                       <  1:240779/239‑1 (MQ=255)
                                                                                                            ccgccGGTGTGGTTTCGTGGAGGTTTGCCGTAGCAGTGGGGGTGGGGGCGATGATCTTCACCGTCGGGGGTGGGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGcc                                                                                                                                  <  2:571145/236‑1 (MQ=255)
                                                                                                              tCCGGTTGGGTTTCGTTGAGTGTTGCCGTGGAGGGGGGGATGGGGCAGTTGGCCTCGCCCGTGGGGGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGa                                                                                                                                <  2:455045/239‑1 (MQ=255)
                                                                                                              gccGGTCTGGTGGCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGa                                                                                                                                <  1:738466/238‑1 (MQ=255)
                                                                                                                       gtgtCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCCTTGGCGGTATTCTCGCCGGTGTTGTGGTGGGCTGGCTGTACCGTCGCTCGCTGCGATTTCCCAGGCGCTGGGGGGGGGGTGAACCCCCCGCGCCGCTCCGCCCGCTGTTCTCGCTGCCATTCGCCTCTTCTTTGATTGcccgaccaacttg                                                                                                                       >  2:504212/1‑228 (MQ=255)
                                                                                                                             ttGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTGTGGTGGGCTGGCTGGACGGTCGCCCGCTGCGGTTCCTCAGCCGCTGGGGCGGGGATGAACCCGCGACGCCGATCGTCCCGCTGTTCCTGCTGCCATTCCCTTCTTTTCTGATTTCCGGACAAATTgggggtt                                                                                                                  >  1:236412/1‑233 (MQ=255)
                                                                                                                                     tGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGCATTCTCGCCGGGTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCCGCCGCTGGGGCGGGGGTGAACCCCCGACCCAGATCGTCCTTCTGTTCCTCCCGCCCTTCCCCTCTTATTTGATTGCCCGACAAATTGGGGTTTCCGGGATcc                                                                                                         >  2:429620/1‑239 (MQ=255)
                                                                                                                                            gCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTGTGGGGGACTGGCTGTACGGGCCCTCCGTCGCATTCCTCAGCCGCTGGGGCGGGGGTGAACCCCCGAGCGCGAGCGGCCCGCCGTTCCTGCCGCCATTTCCCTCTTTATCGATTGCCGAAAAAATTTGCGTTTCCCGcccccccccgcg                                                                                                  >  2:817064/1‑228 (MQ=255)
                                                                                                                                                    gttGGGGACGTTGTTCTTTCCCGTCGGGGGTGGGACGGTCGAATTTATGAAAGTGGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCACCCTCGCTGCGGTTgccg                                                                                          <  2:236412/237‑1 (MQ=255)
                                                                                                                                                           aCGTTGATCTTCCCCTTCGGGGTTGGGGCGGTCGAATTTATGAAATTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGa                                                                                    <  2:820318/238‑1 (MQ=255)
                                                                                                                                                            cGATGATCTTCCCCGTCGGCGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGGCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGTATCCTCGCTGCGGTTGCCGCCGGGAt                                                                                   <  1:802704/238‑1 (MQ=255)
                                                                                                                                                            cGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTTTGGGGAGCTGGCTGTAAGGTCGCTCCCTGCGGTTCCTCAGCCGCTGGGGCGGGGGGGAACCCCCGCGGCGCACCGTCCGCCTTTTCCTCCGGCCATTCGCTTCTTATCTGATTTCCGGAAAAATTTGGGTTTTCCGCATCCCCCCTCCGGTTTCCCCCgggtgg                                                                                  >  2:210067/1‑236 (MQ=255)
                                                                                                                                                                 atCTTCCCCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGGACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGAcc                                                                               <  1:28378/237‑1 (MQ=255)
                                                                                                                                                                     tcgccGTCGGCGGGGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCCCGCCGGTTTTGGGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCAcc                                                                         <  1:164146/236‑1 (MQ=255)
                                                                                                                                                                     tCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTGGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCAcc                                                                         <  1:226730/239‑1 (MQ=255)
                                                                                                                                                                          gggggcgtTGGGACGGGCGAATTTTTGAAGGGTGCCTTTGGTGGTATTCTCGCCGGTGTTGTGGTGAGCGGGCTGTACGGGCGCTCGGTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGGGACGCAGATCGTCCTGCTGTTCCTGCTGCCAGTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTc                                                                    <  2:568097/236‑1 (MQ=255)
                                                                                                                                                                            cggcggTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTTTCCCCGGATTTGTGGTGGGCTGGCTGTACGGGTCCTCGCTGCGGTTCCTCCGCCGCTCGGGGGGGGGTGGACCCGCGCCGCCGAACGTCCCGCTGTTTCTGGTTCCCTTTCCCTCTTTTTTGCTTGCCCCACCAATTTGCGTTTCCGGCACCCCCCCCGCGGTTGCCGCCGGGGGGAAAATCACCCCCTCCg                                                                  >  2:392338/1‑239 (MQ=255)
                                                                                                                                                                                  ttcGAGGGTTGAAGTTATGCAAGGCGCCATTGGCGGTAGTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCCCGCTGCGATTCCTCAGCCGCTGGGTCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGtgtg                                                             <  1:883354/236‑1 (MQ=255)
                                                                                                                                                                                    tcaCGGTGGAATTTTTTAAAGTGGCCCTTGGGGGTTTTCTGGCCGGTTTTGGGGGGGGGTGGCTGGACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGAt                                                           <  2:628466/236‑1 (MQ=255)
                                                                                                                                                                                    ggctgttggAATTTTTGGTAGTGGCTGTTGGCGGTTTTCTCGCCGGTTTGGTGGGGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGAGGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGAtg                                                          <  2:78986/231‑1 (MQ=255)
                                                                                                                                                                                    cGACGGTGGAATTTATGAAAGTAGCCATGGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGAt                                                           <  1:31851/238‑1 (MQ=255)
                                                                                                                                                                                          tCGAATTTATGAAAGGAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCACCACCAGCGccagcgcg                                                             <  1:286635/230‑7 (MQ=255)
                                                                                                                                                                                                  aTGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCt                                            <  1:244296/239‑1 (MQ=255)
                                                                                                                                                                                                   tGAAAGTAGCCATTGGCGGTATTCTCGCCGGGTTTGTGGTGAGGTGGCTGTAAGGTCGCTCGCTTCGATTCCTCAGCCGCTGGGGCGGGGGTGAACCCCCCACGCAGATAGGCCTGCCGTTCCTGCTTCCATTCGCTTCTTTTCTGCTTGACGCACCTATTTGCGTTTCCGGCATTCTCCCTGAGGGTTCCTCCGGGACGACCCTCCACCCCGCCCGGGTGAagcgccgggtgcccccg                                           >  2:136908/1‑235 (MQ=255)
                                                                                                                                                                                                                   gggTTTTCCCGCCGGTTTGGTGGGGGGCCGGCGGTGCGGGCCGTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGt                            <  2:571357/237‑1 (MQ=255)
                                                                                                                                                                                                                     gttttttcGCCGGTTTTTGGGTGGGCGGGGTGTCCGGTCGCTCCTCGCTTTTCCTCCGCCGCTGGGGGGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCGGCCATTCGCGTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCa                         <  2:581353/233‑1 (MQ=255)
                                                                                                                                                                                                                       aTTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGGTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCaaa                       <  1:210067/239‑1 (MQ=255)
                                                                                                                                                                                                                       aTTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCaaa                       <  1:85433/239‑1 (MQ=255)
                                                                                                                                                                                                                        ttCCCGCCGGTTTTGGGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCCGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCaaa                       <  1:688625/238‑1 (MQ=255)
                                                                                                                                                                                                                                   tttGGGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTggg            <  1:136908/238‑1 (MQ=255)
                                                                                                                                                                                                                                            aGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGGGCAAACAGCACCTGGGCGATGCTGGa  >  1:53268/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                
GGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATGAAAGTAGCCATTGGCGGTATTCTCGCCGGTTTTGTGGTGAGCTGGCTGTACGGTCGCTCGCTGCGATTCCTCAGCCGCTGGGGCGGTGATGAACCCGCGACGCAGATCGTCCTGCTGTTCCTGCTGCCATTCGCTTCTTATCTGATTGCCGAACATATTGGCGTTTCCGGCATCCTCGCTGCGGTTGCCGCCGGGATGACCATCACCCGCTCCGGTGTGATGCGCCGTGCGCCGCTGGCAATGCGCCTGCGTGCAAACAGCACCTGGGCGATGCTGGA  >  NC_000913/4280342‑4280815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: