Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	100%	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	83 [0]	[0] 83	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	77 (1.540)	57/414	NT	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

TCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAA  >  NC_000913/4001418‑4001867                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                tCCCGGTCATGCTGAGCCCATTGCAACGGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGACCCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATc                                                                                                                                                                                                                                <  1:494867‑M1/228‑2 (MQ=255) tCCCGGTAATGGTGAGCGCATTTCACCTGGAAAATAACCGACTGGCCCGGCGGGAAGTCGAAGAGTCACAACCCCTGGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATc                                                                                                                                                                                                                                <  1:624859‑M1/228‑2 (MQ=255)    cGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTTGACCGGCTTGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATct                                                                                                                                                                                                                               <  1:493022‑M1/226‑3 (MQ=255)          tGCTGACTGCATTTCAGATGGAAAATTACCGGCTGACCGGGGTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttctt                                                                                                                                                                                                                        <  1:291773‑M1/227‑10 (MQ=255)           gCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATAGGCACGGTTCTTTGAAGACGAGGACGGCCTGCATatccttctttt                                                                                                                                                                                                                      >  1:617358‑M1/1‑217 (MQ=255)                    aTTTCAACTGGAAAATAACCGTCTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaag                                                                                                                                                                                                             <  1:592810‑M1/228‑21 (MQ=255)                      ttCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGCCGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGACTCGGCACGTTTCTTTGAAGACGACGACGGCCTCCATctccttcttcttctttgaaga                                                                                                                                                                                                            >  1:278537‑M1/1‑206 (MQ=255)                         aatgggaacaTAACCGATTGACCCGGCGGGAAATCGAAGGGTCACAACCCCTTGGAAATGCCGGATGGATTGATCTTGTCGAACCGGACGGCGACGGGCGGCTGCGCGTACAATCTGAACTTGGCCAGAGCCTTGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcg                                                                                                                                                                                                        <  2:432940‑M1/224‑26 (MQ=255)                         aaCTGGAAAATACCCGGCTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAAGGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcg                                                                                                                                                                                                        <  1:605571‑M1/228‑26 (MQ=255)                                aaaaacccgggtgacccgggcggAATTCGAAGGTGCACTACCCCTTGTAAATGCGTTATGGATTGCTCTTGTCGAACCGGAACACGACGGCGGCCTGCGCGTACAATCTGAACTTGGCCAGAGCCTTGCACCCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatc                                                                                                                                                                                                 <  2:359348‑M1/217‑33 (MQ=255)                                   taacccgtcgccccggggggacgtcGAAGAGTCACAACCCCTTGGAAATGCAGTATGGATTGATCTTGCCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacg                                                                                                                                                                                              <  2:618233‑M1/217‑36 (MQ=255)                                        gACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCTCGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGGATCGGCACGTTTCTTTGAAGCCGACGAAGGCCGGCACctccttcttcttctttgaagatgcggaaggtacagccggt                                                                                                                                                                                         >  1:324897‑M1/1‑188 (MQ=255)                                        gACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑ACGACATCGAAGCATCGGCACGTTTCTTTGAAGCCAACGACGGCCGGCCTctccttcttcttctttgaagttgcggaagagcaccccgga                                                                                                                                                                                         >  2:213421‑M1/1‑188 (MQ=255)                                        gACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggt                                                                                                                                                                                         <  1:421558‑M1/228‑41 (MQ=255)                                         aCTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGACCGTTTCTTTGAAGACGACGACGGCCTGCATctccttctccttctttggatgtgcggaaggtcaatccggga                                                                                                                                                                                        >  1:600007‑M1/1‑187 (MQ=255)                                             aCCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCCCGTTTTTTTGACGACGACGACGCCCTGCATctccttctctttctttgaagatgcggaaaatacccccggtaactc                                                                                                                                                                                    >  2:447265‑M1/1‑183 (MQ=255)                                                 ggCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGGGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccact                                                                                                                                                                                <  1:88909‑M1/228‑50 (MQ=255)                                                  gCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttctttttctttgaagatgcggaagatcacgccggtaaacccaccg                                                                                                                                                                               >  2:542253‑M1/1‑178 (MQ=255)                                                           cGAAGAGTCCACACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcattta                                                                                                                                                                      <  1:338309‑M1/228‑60 (MQ=255)                                                              agggtccaaaccccttgtAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGCCGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttacc                                                                                                                                                                    <  1:431310‑M1/219‑62 (MQ=255)                                                               ggttacccaccccttggAGATTGATTAGGGATTGATCTGGTCGAACCGGGCGACGGCGAGCACCTGCGGGTACAATCTGAAATTGGCCAGGACCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAATCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaggatcacgccggtaactccactgtggcatttacca                                                                                                                                                                   <  2:257321‑M1/219‑63 (MQ=255)                                                                    acaACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatc                                                                                                                                                                 >  1:400398‑M1/1‑160 (MQ=255)                                                                       aCCCCTTGGATATTGAGTATGGATTGATCTTGGTGAACCGGACGACGACGGGCGCCTGCGTGTACAATCGGAAGTTGGCCAGAGCCGGGCAACCCGCCCGGAACTGG‑AAGACAACGAAGCATCGGCAAGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatg                                                                                                                                                          <  2:278537‑M1/228‑72 (MQ=255)                                                                        ccccTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatg                                                                                                                                                          <  1:542253‑M1/227‑72 (MQ=255)                                                                            ttGTAAATGCAGTCTGGATTGACCTTGGCGAACCGGACGACGACGAGCGCCTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcg                                                                                                                                                      <  1:343665‑M1/227‑76 (MQ=255)                                                                              gTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtc                                                                                                                                                    <  1:204809‑M1/227‑78 (MQ=255)                                                                                     caggagggattgacCTTGCCGAACCGGACGACGACGAGGGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttact                                                                                                                                            <  1:436636‑M1/222‑86 (MQ=255)                                                                                        tATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagataacgccggtaactcccctgtggcatttaccatccgggatgttcgtctgtttactct                                                                                                                                          >  1:518067‑M1/1‑140 (MQ=255)                                                                                           ggATTGATCTTGGCGACCCGGACGACGACGAGCGACTGCGCGTACAATCGGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcg                                                                                                                                       <  1:328915‑M1/227‑91 (MQ=255)                                                                                                  tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCCTctccttcttcttctttgaagatgcggaaaatcacgccggtaactccactgtggcatttaccatccgggatggccgctggtttcctctccctgaggg                                                                                                                                 >  2:52743‑M1/1‑130 (MQ=255)                                                                                                  tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaggatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctccgtgacgctg                                                                                                                               >  1:654960‑M1/1‑130 (MQ=255)                                                                                                  tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagctcacgccggtaactccactgtggcctttcccatccgtgatggtcgtctgttttctctgcgtgagaggg                                                                                                                               >  2:366493‑M1/1‑130 (MQ=255)                                                                                                  tCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtg                                                                                                                               >  1:129653‑M1/1‑130 (MQ=255)                                                                                                  tCTTGTCGAACAGGACGACGACGAGCGACTGCGCGTAAAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGTAACTGG‑AAGACATCGCAGCATCGGCACGTTTCTTTGAAGACGAGGACGGCCTGCATctccttcttcttctttgaagaagcgggagatcacgccggtaactcccctgtggcatttaccatacgtgctgggcgtctgtttacgctgcgtgagcgtg                                                                                                                               >  2:76342‑M1/1‑130 (MQ=255)                                                                                                      gTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagttgcggaagttcccgccggtaactcccctgtggcatttaccctccctgctggccgcctgtttctcctccgtgggggtgaacc                                                                                                                           >  2:587687‑M1/1‑126 (MQ=255)                                                                                                      gTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcg                                                                                                                                       <  1:202158‑M1/216‑91 (MQ=255)                                                                                                      gTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcg                                                                                                                                       >  2:202158‑M1/1‑126 (MQ=255)                                                                                                      gTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccgggaactccactgaggcatttaccatccgtgatggtggtctgtttactctcgctgagcgggaact                                                                                                                           >  1:428643‑M1/1‑126 (MQ=255)                                                                                                        ccaaccGGACGACGACGAGCCACTGCGCTTACAATCTGAACTTGGCCAGGGCCTGGCACCCCGCCCGGACCTGG‑AAGACATCGAAGCATCGGCCCGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactg                                                                                                                          <  1:402961‑M1/225‑104 (MQ=255)                                                                                                         gAACCGGACCGCGACGGGCGGCTGCGTGTACAATCGGAACTTGCGCAGAGCCTGGCACCCCGCCCGAGACTGG‑AAGACATCGAATCATCGGCAAGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcc                                                                                                                        <  2:427723‑M1/228‑106 (MQ=255)                                                                                                           aCCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttcctctgcgtgagcgtgaactg                                                                                                                          >  1:159329‑M1/1‑121 (MQ=255)                                                                                                            ccGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactttggcatttaccatccgggctggtcgtctgtttattctccgtgagcgggaactgccccc                                                                                                                     >  2:151770‑M1/1‑120 (MQ=255)                                                                                                              ggACAACGACGAGCGACGGGGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctttttactctgcgtgagcgtgaactgcccgctt                                                                                                                   <  1:587687‑M1/228‑111 (MQ=255)                                                                                                               gacgacgacGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccggtagtgtcgtctgtttactctgcgtgcgcgtgaactggccgcttt                                                                                                                  >  2:467428‑M1/1‑117 (MQ=255)                                                                                                                acgacgacgAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcggcgttttactctgcgtggccgggaactgccccctttt                                                                                                                 >  2:620545‑M1/1‑116 (MQ=255)                                                                                                                    cgccggccgcttgcgcgtaacatCTGAATTTGGCCACGGCCTGGCAACCCGCCCGGACCTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGCCGACGACGGCCTGCTTctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtc                                                                                                             <  2:324897‑M1/217‑117 (MQ=255)                                                                                                                                           cTGAACTTGGCCGGGCCCTTGCCCCCCGCCCGGAACTGG‑AAGACAACGAAGCACCGGCACTTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttcttgtaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagc                                                                                      <  2:518067‑M1/228‑140 (MQ=255)                                                                                                                                           cTGAACTTGGCCAGGGCCTGGCCACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagc                                                                                      <  1:54699‑M1/228‑140 (MQ=255)                                                                                                                                           cTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGA‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagc                                                                                      <  1:568115‑M1/228‑140 (MQ=255)                                                                                                                                              aaCTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCGCGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggcagatcacgccggcaactccactgtggcctttcccatccgtgatggtcgtctgtttaccctgcgtgagcgtgacctgcccgcttttcttctttctggtatgggtgcccggagccag                                                                                   >  2:444663‑M1/1‑86 (MQ=255)                                                                                                                                               aggtggcCGGACCCTGGGAGCCCGGCCGGAACGGG‑AAGAACTCCAACCTTCGGCACCTTTCTTTGAAGGCGACGCCGGCCCGCATctccttttttgtctttgaagatggggaagatcacgccggtaactcctctgtggcatttaccatccgtgatggtcgtttgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagt                                                                                  <  2:428643‑M1/225‑144 (MQ=255)                                                                                                                                               aCTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttcccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtaccgtatgcgtgcccgtagccaat                                                                                  >  2:566747‑M1/1‑85 (MQ=255)                                                                                                                                                    gCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccctccgtgatggtcgtctgtttactctgcgggagcgtgaacggcccgcttttcgtcggtatcgtatgcgtgcccgtagcccttcgat                                                                              >  2:152353‑M1/1‑80 (MQ=255)                                                                                                                                                    gCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttcctctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgat                                                                              >  1:476468‑M1/1‑80 (MQ=255)                                                                                                                                                    gCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcggctgtatcgtatgcgtgcccgtagccagtcgag                                                                              >  1:166761‑M1/1‑80 (MQ=255)                                                                                                                                                    gCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgcctgtattgaatccgtgcccgtgaccagtcgat                                                                              >  1:576818‑M1/1‑80 (MQ=255)                                                                                                                                                    gCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgcctgtatcgtatgcgtgcccgtagccagtcgat                                                                              >  1:281732‑M1/1‑80 (MQ=255)                                                                                                                                                    gCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactcc                                                                                                                                                                                   >  2:211764‑M1/1‑80 (MQ=255)                                                                                                                                                     ccAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactcca                                                                                                                                                                                  <  1:211764‑M1/126‑48 (MQ=255)                                                                                                                                                         agCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttattctgcgtgagcgggaactgcccgcttttcggctgtatcgtatgcgtgcccgtgcccagacactgggcac                                                                        >  2:532898‑M1/1‑75 (MQ=255)                                                                                                                                                              ggCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcatgcccgtagccagtcgatggtagacggta                                                                   >  1:301990‑M1/1‑70 (MQ=255)                                                                                                                                                              ggCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggta                                                                                                                                                                                        >  1:526882‑M1/1‑70 (MQ=255)                                                                                                                                                              ggCAACCCGCCCGGAACTGG‑AAGACACCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggta                                                                   <  1:467428‑M1/228‑159 (MQ=255)                                                                                                                                                               gccccccccccggaacTGGAAAGAC‑TTGAAGCCTCGCCCGGTTTCTTTGAAGACGGCGCCGGCCGGCATctcctttgtcttctttgaagatgcggaagatcacgccggtaactccactggggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaa                                                                  <  2:600007‑M1/224‑160 (MQ=255)                                                                                                                                                               gCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaa                                                                                                                                                                                       <  2:526882‑M1/111‑43 (MQ=255)                                                                                                                                                                   cccgcccgGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgc                                                               >  1:34216‑M1/1‑65 (MQ=255)                                                                                                                                                                          gggactgG‑AAGACATCGAAGCATCGGCACGTTTTTTTGAAGGCGTCGCCGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactcccctgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagt                                                       <  2:617358‑M1/225‑171 (MQ=255)                                                                                                                                                                            aaCTGG‑AAGCCATCCAAGCATCGGCTGGTTTCTTTGAAGACGCCGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagtt                                                      <  1:45627‑M1/227‑172 (MQ=255)                                                                                                                                                                            aaCTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcccgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagtt                                                      <  1:447265‑M1/227‑172 (MQ=255)                                                                                                                                                                             aCTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcggatgcggtcccgtagccaggcgagggtagagggtaacccctacgagttgt                                                    >  2:457076‑M1/1‑55 (MQ=255)                                                                                                                                                                                 ggaagcctccgaagcatcgGCACGTTTCTTTGAAGGCGACGACGGCCTGCATctcctgcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgc                                                 <  2:181964‑M1/219‑177 (MQ=255)                                                                                                                                                                                        aTCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccaccgtggcctttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcttatgcgtgcccgtagccagtcgcgggtagccggtaacgcctacgcgttgctgctgggtct                                          >  2:342435‑M1/1‑45 (MQ=255)                                                                                                                                                                                                   ggcgcttttctttgaAGACGACGACGGCCTGAATctccctcttcttcttggaagatgcggaagagcacgcgggtaaccccacgggggcatttaccatccgtgatggtcgtctgttttctctgcgtgagcgtgaactgcccgcttgtcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaacca                               <  2:476468‑M1/222‑195 (MQ=255)                                                                                                                                                                                                          ttCTTTGAAGACGACGACGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtatacggtaacgcctcacagttgctgctggatctgttcgaaaccaaaaagga                        >  2:64813‑M1/1‑27 (MQ=255)                                                                                                                                                                                                             tttGAAAGCGACGCCGGCCTGCATctccttttgcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaaca                     <  1:213421‑M1/228‑205 (MQ=255)                                                                                                                                                                                                                   gacgacgacGGCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggc               <  1:151770‑M1/228‑211 (MQ=255)                                                                                                                                                                                                                           cgGCCTGCATtcccttcttcttctttgaaggtgcggaagatcgcgccggtaactccacggtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaa       <  2:576818‑M1/228‑219 (MQ=255)                                                                                                                                                                                                                             gCCTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaat      <  1:620545‑M1/227‑220 (MQ=255)                                                                                                                                                                                                                              ccTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtc                                                                                                                                                       >  2:596379‑M1/1‑7 (MQ=255)                                                                                                                                                                                                                               cTGCATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcg                                                                                                                                                      <  1:596379‑M1/81‑76 (MQ=255)                                                                                                                                                                                                                                ttcatctccttcttcgtccttgaagctgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaattgaa  <  1:271357‑M1/226‑224 (MQ=255)                                                                                                                                                                                                                                tGCATttccttctgtttctttgaagatgcggaaggtcccgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcgtgcccgtagccagtcgatggtagacggtaacgcctacgagttgctgctggatctgttcgaaaccaaaatcgaacagttggcagatgaaattgaa  <  1:221369‑M1/228‑224 (MQ=255)                                                                                                                                                                                                                                  cATctccttcttcttctttgaagatgcggaagatcacgccggtaactccactgtggcatttaccatccgtgatggtcgtctgtttactctgcgtgagcgtgaactgcccgcttttcgtctgtatcgtatgcttgcccgtagccagtcgatggtagacggtaacgcctacgcgttgctgctggatctgttcgaaaccaaaatcgaaaagttggaaggtaaaattga   >  2:291038‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                TCCCGGTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGG‑AAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAA  >  NC_000913/4001418‑4001867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑