| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,053,515 | T→C | 26.8% | S2G (AGC→GGC) *442W (TGA→TGG) |
ubiH ← pepP ← |
2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,053,515 | 0 | T | C | 26.8% | 57.3 / 3.4 | 37 | S2G (AGC→GGC) *442W (TGA→TGG) | ubiH pepP | 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base T (11/15); new base C (9/2); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.65e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAT > NC_000913/3053292‑3053741 | aTGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGCCTTAACCGGGAAATAGCCAGAG‑CCCGCGTCG‑CGCCC‑GCCCTGCCGCCACCGACCATGATTACGCCCAtt > 2:115327/1‑228 (MQ=255)aTGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAACG‑CCAGCGCCG‑GGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCAt > 1:183737/1‑227 (MQ=255) aGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCCTGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATCCCGCCACCGACGATGATTACCCCCCTTGCtt > 2:414270/1‑227 (MQ=255) aGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATACCCACCG‑CCAGCGCCC‑CGCCC‑GCCCTGCCCCCACCCGCGGTAATTACCCCCAttcgct > 2:104070/1‑223 (MQ=255) ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGCTGATTACGCTCCATGCGTtc > 1:226932/1‑227 (MQ=255) ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGAATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGCCTTAACCGGGAACTAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCCCCACCGACCATGATTACGCCCATTGCTTtct > 1:95320/1‑228 (MQ=255) tCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGCCTTAACCGGGAAATAGCAAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCCCCGCCGATGATTACGCTCATTGCTTTCCCccag‑ca > 1:314571/1‑227 (MQ=255) tGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATCCCGCCACCGCCGATGATTACCCCCCTTGCTTTCTCGCAC‑CAccccccacccctt > 2:658106/1‑218 (MQ=255) aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTcc > 1:520606/1‑227 (MQ=255) aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAA‑CAACCCTCAACGCCTCGGTTACTTCCg > 1:282698/1‑228 (MQ=255) gACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTC‑‑CGCCCGGCCCTGCCTCCACCGCCGATGGTTCAGCCCATTCCTTT‑TCGCCGCCAACCACCAACCCTTCGATTTCTTCCGTCTGTTCCCCCACACt > 2:5883/1‑228 (MQ=255) cgccAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGCCG‑CGCCC‑GCCCTGCCCCCACCGACGATGATTCCGCCCATAGCTTTCTCTCAG‑CAACCCTCAACGCTTCCATTTATTCCCGCGTTTTCACCACGCTGACGGGGAggggc > 2:373984/1‑225 (MQ=255) aTCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCCCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGAGGTTTTCGTTACCg > 1:366675/1‑227 (MQ=255) ctttctCCCTCAAAACCCGGGTGGAGCGTGGGACTGGGGCGCAGGCGCTTAAATCAAGTGGCCGTGCAGCCCCCC‑GTGACTGAACCGGAAAATGGCCACCG‑CCAGCCTCG‑ACCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGtt < 2:314571/223‑1 (MQ=255) cGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGAAAGGGTTTAGTTAACGGTTTTgg > 2:558851/1‑228 (MQ=255) aaaGCCGGGAG‑GAGCCTGTGACTCGGGCGCCGTCGCTTCAATCAAATGGCCCGGCCGCCCCCC‑GTGAATTAACCGGGAAATAGCAAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATcaca < 1:610398/228‑1 (MQ=255) gAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGCCGGTGAGGTTTTCTGTTCCGGTTTTGG‑TAATCACAATtttgtc > 1:405398/1‑228 (MQ=255) tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACAACGCTGGCGGTGAGGGTTTCTTTTCCCGTTTCTG‑GAATCACACAGTTGGCTTCCATTAGAATTCCg > 1:644964/1‑228 (MQ=255) tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCACCAACGCCTCGATTTCTTCCGGCTTTTTCACCCCGCTGGCGGCGTGGTTTTCGTTTCCGGGTTCGGATACGCACAA‑GTCGGCTTCAAACCAAATGcca > 2:7477/1‑227 (MQ=255) gcggAGTCTCTTCAACAAAATG‑GCCGGGAGCCCCCCGGTGCTCTAACCGGGAAA‑AGCCCGGGCCCAGCGTCG‑GCCCC‑GCCATGCCGCCACCGGCGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGa < 2:95320/227‑1 (MQ=255) gcctgatCGCTTAAATCAATGGTCCCGGAAGCGCCCC‑GTGATTTAACGGGGAACTCCCCAGCG‑CCCGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGa < 1:364864/221‑1 (MQ=255) tCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGGGGTTTTCTTTTCCGTGTTCGG‑TACTACCAATATCGTCTTCATAACAAAAGCCggcaacccg > 2:572965/1‑220 (MQ=255) aTGTCCCGGAAGCCCCCG‑GGGACTTAACCGGAAAATGCCCAGCG‑CCAGGTGCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTgg < 1:115327/227‑1 (MQ=255) gcCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTTCTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCCAGACCGCGATATTGTTTTTGCACTTTTTCATCCg > 1:418894/1‑228 (MQ=255) cccc‑GTGACTTAACCGGGAAATAGCCAGCG‑CCCGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCgg < 1:414270/228‑1 (MQ=255) cggTGACTGAACAGGGAATTACCCAGCG‑CCGGCGCCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGATCATTGCTTTCCCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgcg < 1:19642/226‑1 (MQ=255) cGGGAAATGGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGATTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGcc < 1:104070/228‑1 (MQ=255) ccgccgcg‑cccgccTCC‑CGCCC‑GCCATGCCGCCACCGCCGATGATTACGCTCATTGCTTTCGCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct < 1:592296/223‑1 (MQ=255) gcccccg‑cccgcgTCGCCCCCC‑GCCATGCCCGCACCGATGCTGTTTACCCTCAGTGCTTTCCCGCAG‑GACCCATAAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct < 2:282698/223‑1 (MQ=255) cgccc‑gccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGTAAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc < 1:558851/228‑1 (MQ=255) gccc‑gtcATGCCGCCACCGACGGTGATTACGCCCATTGCTTTCGCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc < 1:60520/227‑1 (MQ=255) cccc‑ggcATGCCGCCACCGACGATGATTACGTTTATTGCTTTCCCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATg < 1:339067/224‑1 (MQ=255) gccgttcatTACGCTAATTTCTTTCCCGCGA‑CCACCATCATCGCTTGGATTTCTTCCGGCTTATTCCCCGCGCTGGCTGGGAGGTTTTTGTTGCCGGTGTCGG‑AAATCACAATGTCGTCGTCGATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCGCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgag < 2:578370/221‑1 (MQ=255) ttACGCCCATTGCTTGTCCGGCG‑CAACCATTAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTg < 1:5883/228‑1 (MQ=255) tccGCTCCTTGCTGTCCCTGAG‑CAGCCATAACCTCTTGGTTTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCCGAATTTCGTCTTCAATACTAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCGAGAATGCGCGAGCGATCCTGa < 2:478561/226‑1 (MQ=255) cTCATTGGTTTCCCGCAG‑CAACCATCCACGCTGCGATTTCGTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAt < 1:222791/228‑1 (MQ=255) cTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑Taa > 2:525120/1‑93 (MQ=255) tCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAAt < 1:525120/93‑1 (MQ=255) | ATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGAT‑GAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCC‑GTGACTTAACCGGGAAATAGCCAGCG‑CCAGCGTCG‑CGCCC‑GCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAG‑CAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGG‑TAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAT > NC_000913/3053292‑3053741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |