Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,346,885 | C→T | 6.1% | P103L (CCG→CTG) | ptsN → | sugar‑specific enzyme IIA component of PTS |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,346,885 | 0 | C | T | 6.1% | 188.8 / 3.7 | 69 | P103L (CCG→CTG) | ptsN | sugar‑specific enzyme IIA component of PTS |
Reads supporting (aligned to +/- strand): ref base C (27/36); new base T (2/2); total (31/38) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 |
AAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAA > NC_000913/3346660‑3347108 | aaaaaCGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCgg < 1:121794/228‑1 (MQ=255) gcgcCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGAGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGACGATGGCGTTTTCGTTCAGATGGAAACGACTATAGCTTTCGATGCCCACGAAAACCAACCGGTggca > 1:303027/1‑225 (MQ=255) atcaGCGAGGCGGGGGCGAAACAACGTAGCCTGCCTGCTCAGGTGGTTTTGGAAGCTAT‑CCTGACGCGGGAAAAAAGTGGCAGGACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCctg < 1:39660/228‑1 (MQ=255) atcaGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGCCTTACTTTTTGCCctt > 2:172561/1‑227 (MQ=255) gggcggcGAAAAAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCgg < 1:419443/227‑1 (MQ=255) aaaCACTTTGGCCTGCGGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCa < 1:9338/227‑1 (MQ=255) aGCCGGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTCTTGCCATTCCGACTGGCCCACTGGAAGCCGCTCCTCTGCGCGCCGTTGGCGGTTTCGTTCAGCTCGAACAGCCTCTAGCTTTCGCTGCCATGGACACCCCCCCGGTGGAATTCCTTTTTGCCCTGCCGGTGCCGGCCGACAAAACCaaaaa > 2:45111/1‑226 (MQ=255) gcctgcctCCTCATGTGGTTTTTGACGCTAG‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTTCCGGCAATGGTATTGCCGTTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACg < 2:587286/227‑1 (MQ=255) gCCTGCGGCCGTGGGTGGTTGTTGAAGCTAT‑CTTGACACGGGAAAAAATGGGAAGTACCGTTCTCGGCAAGGGTATTGCGATTCCGCATGGCAAACTGGAAGAAGATACGCGGCGCGCCGTGGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTGCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACg < 2:141973/227‑1 (MQ=255) ccTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCCAAACGCCTATAGCTTTCGATGCCACCGACAACCAACAGGCGGACTTCATTTTTGCCCTGCTGGTGCCGGCAGACCAACCTaaaaaacc > 2:609936/1‑224 (MQ=255) ccTGCCGCCTCAGGGGGTTTTTGAAGCTTT‑CCTGGCGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 2:489957/228‑1 (MQ=255) ccTGCCGCCTCAGGGGGTTTTCGAAGTCTG‑CCGTACCCGGGAAAAAATGGTCAGCACCGGGATCGGCAATGGTAGTGCTATTCCGCATGGCAAACTGGAAGAAGATATCCCGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 2:351083/228‑1 (MQ=255) ccTGCCCGCTAAGGTGGTTTGTGAAG‑TATACCTGACGGGGGAAAAAATGGGAAGTACCGGTATCGCCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 2:551141/228‑1 (MQ=255) cTGCCGGCTCAGGTGGTTTTTGAAGCGAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTAACGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 1:344917/227‑1 (MQ=255) cgccTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTg > 2:658047/1‑227 (MQ=255) gcctgcggtggtTTTTGAAGCTAT‑ACCGCCGCGGGAAAAAATGGGCAGTACCGGTATCGGCAAGGGTATTGCCATTCCGAATGGCAAACTGGAAGAAGATTCTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCa < 2:660023/222‑1 (MQ=255) tcTCAGGTGGTTTTTGAAGGTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCAt < 1:239399/227‑1 (MQ=255) ttttGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGGCAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCt > 1:268866/1‑228 (MQ=255) tAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACAAAACTAAAACGCACCTCCTTACCCTGTCCCtgtgggccaa > 2:87939/1‑220 (MQ=255) gcgggggAAAAAATGGGCAGTACCTGTAGCGGGATTGTTATTGCCGTTCGGCAGTGCAAACTGGAAGAAGATGCCCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACGTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTg < 2:551580/224‑1 (MQ=255) tgAAAAAATGGGCAGGCCCGGTATCGGCAATGGTAGTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTTCGCGCCGTTGGCGTTTCCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGa < 2:169646/227‑1 (MQ=255) ggAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGCTACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCAGGTGTCGAAACGGCTGGCgg > 2:347388/1‑227 (MQ=255) ggAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTAGCAACGCCTATAGCTTTCGATGCCATCGACAACCCAACGGTGGACTTACTTTTTGCCCTGCTGGTGCGGGCAGACCAAACCAAAACACACCTCGATACACTTTGGCTGGTGGCGAACAGTCTTGCgg > 2:459644/1‑227 (MQ=255) ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCACACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgg > 1:651485/1‑227 (MQ=255) ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGTTGGTGGCGAAACGTCTGGCGGACAAAACCACCTGCCCCCGTTTAcgc > 2:561255/1‑228 (MQ=255) ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGCCCAAACTAAAACGCACCCTCATACTCTGTCGCTGGTGGCGCAAGGTCTGGCGGACAAAACAATCTGCCGCCGTTTAcgc > 2:230422/1‑228 (MQ=255) gCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAACTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg > 2:257766/1‑228 (MQ=255) gtaTGGTTTTGCCTTGCCGCAAGGAAAACTGGAAGGAGATTTGCGGCGAGGCGTTGGCGGTTTCGTTCAGCCCGAAATGCCTATGGCTTTCGAGGCCATCGACAACCAACCGGTGGACGTTCGTTTTGCCCTGCTGGTGCCGGCAGACCAAACTCAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 2:432724/225‑1 (MQ=255) gaaTGGTTTTGTCATTCCGCAGGGCAAACTGGAAGAAGATACTGTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 2:226614/226‑1 (MQ=255) cAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATCCTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 1:105185/227‑1 (MQ=255) aaaTGGTATGGCCATTCCGCAGGGCAAACTGGAAGAAGATTCCCCGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 1:316976/226‑1 (MQ=255) tttttgcATTCCGCCTGGCAAACTGGAAAAAGTTACTTTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCa < 2:161629/222‑1 (MQ=255) ttGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGCCCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGGCTGGGGGACAAAACCATCTCCCCCCGTTTACCcccagcccagg > 2:329782/1‑227 (MQ=255) ttGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATATTCCGTCGCTGGTGTCGAAACGTCTGCCGGCCAAAACCATCTGCCGCCGTTTACCCGCAACCCaca > 2:439536/1‑226 (MQ=255) cATTCCGCATGGCAAACTGGAAGAAGATATCCGGGGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGGCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTTGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCg < 1:230422/227‑1 (MQ=255) ttCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATg < 1:329782/228‑1 (MQ=255) tCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGCCAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGGTGa > 1:438881/1‑228 (MQ=255) tCCGCAAGGCAAACTGTAAGAAGATACTCTGCGCGCGGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACCGGGGGACTTACTTTTTTCCCTGCTGGGGCCGCACGACCAACCTAAAACGAACCAGACTACTCTATGCCTAGTGGCGAAACACTTGACGGACAAAACAAACTGCCGCCGTTTAACGGCAGCCCAGAGCgggga > 2:442950/1‑224 (MQ=255) ccGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACACCCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGGGCCACCCAGAGCGagtaa > 1:53028/1‑224 (MQ=255) gaTGGAAAACTGGAAGAAGATACGCGGGGCGCCGTTGGCGTTTTCGTTCAGCTCGAATCGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAagag < 2:438881/227‑1 (MQ=255) cgggCAAAATGGAAAAAGATACCGTTCGCGCGGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCCTCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGc < 2:331376/226‑1 (MQ=255) tGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGTTGAAGAGCt > 1:72437/1‑228 (MQ=255) cAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCAACTGCCGCCGTTTCCGCGACCCCCGGAGCGATAGAGAGCTGt > 2:402464/1‑227 (MQ=255) tGGAAGAAGATACTCTGCGGGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCaaa < 1:389554/228‑1 (MQ=255) gaagagGATTCCCTGGCCGCCGTTGGCGTGTTCGTTCAGCTCGAAACTGCTAATGATTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAAt < 1:261005/227‑1 (MQ=255) tctGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTCTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGGAGACGAGATGAATCAAATACTTACgagtaa > 2:73641/1‑223 (MQ=255) tGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGAt > 2:423725/1‑197 (MQ=255) gcgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATg < 1:423725/197‑1 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGc > 1:32957/1‑192 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGTCGGACAAAACCATCTGCCGCCGTTTACGCGCCACCCCGAGCCGTGAAAAGCTGAATCAAAACATTCCCGAAACCGaa > 2:248965/1‑228 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCGGAGCGATGAAAAGCTGTATCAAATCATTACGGATACCGaa > 1:121907/1‑228 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGac > 1:549190/1‑227 (MQ=255) gcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCg < 2:32957/192‑1 (MQ=255) gTTGGCCTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACt < 1:55919/228‑1 (MQ=255) ttCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACCAAACCATCTGCCGCCGTTTACCCGCAGCCCAGAGCGATGAAGAGCTGTATCCAATCATTACGGATACCGAATGGACTCCGGATaaa > 2:401749/1‑228 (MQ=255) tCGTTCAGCTCGAAACGCCTTTAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGGCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAg < 2:557753/228‑1 (MQ=255) cTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACa > 1:616779/1‑123 (MQ=255) tataGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACaa < 2:616779/123‑1 (MQ=255) ttaGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt < 1:347388/226‑1 (MQ=255) attgCTTTCGCTGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTTCATACTCTGCCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt < 1:520404/224‑1 (MQ=255) ataGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt < 1:257766/227‑1 (MQ=255) taGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGtct < 1:405191/228‑1 (MQ=255) ttCGATGCCATCGACACCCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGAAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGAACTCCGGATGAAGCGTCGTTATTCGGTAAGGTCTCtttt > 1:187907/1‑228 (MQ=255) tCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGTTACCGCAGGGACTCCGGATGAACCGCAGTTATTCGGTAATGTATCTTTTa > 2:83119/1‑228 (MQ=255) tgATGCCCGCGACCACCAACCGGTGGCACTACGTTTTGCCCTGCTGGCGCCGGCAGACCAAACTAAAACGCACCTGCATGCGCTGTCGCTGGTGGCGAAACGTCGGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGAGGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAg < 2:392818/227‑1 (MQ=255) cATCGACAACCAACGGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGTTGACGAGCTGTATCAAATCCTTACGGATACCGAAGGAACTCCGGAGGAAACGTAGTTTTTCGGAACTTTCTCCTTTaagctgtg > 2:361847/1‑221 (MQ=255) cGACAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTtgtg < 1:73641/227‑1 (MQ=255) gcaaccaacCGGTGGACTTCCTTTTTGCCCTGCTGGGGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTgagg < 1:432318/227‑1 (MQ=255) acaacaAACCGGTTGCCTTTCTTTTGGCCCGGGTGGGGCCGGCCGGCCAAACTAAAACGCACCTGCATACTCGGGCGCTGGTGGGGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATTATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTgagg < 2:268866/228‑1 (MQ=255) caacgGGGGGACTTTCTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGaaa < 2:549190/228‑1 (MQ=255) aaccGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGGCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGaaa < 1:658047/227‑1 (MQ=255) accGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCt > 1:501998/1‑87 (MQ=255) ccGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTg < 2:501998/87‑1 (MQ=255) | AAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAA > NC_000913/3346660‑3347108 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |