Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,296,060 | C→T | 32.7% | intergenic (+266/+376) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,296,060 | 0 | C | T | 32.7% | 28.1 / 41.7 | 49 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Reads supporting (aligned to +/- strand): ref base C (21/12); new base T (6/10); total (27/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.44e-01 |
ATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGC‑GTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGC > NC_000913/4295840‑4296273 | aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAACGTG‑ATTTGGTAGTTCGGATAAGGCGTTTACGCCGCATCCGCACTCAATGCCTgt > 1:573198/1‑226 (MQ=255) gCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCACCATCAATGCCCGAGCGGACGCTTGc > 2:646170/1‑228 (MQ=255) aTCCGGCCTGCGCAAGCCAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt < 1:496851/228‑1 (MQ=255) aTCAGGACTACGCAGGACAGCGGAATAGCCTGATTTAGCGTGATTTTGGCGGTCGGATAAGGGATTTATGCCGCATCCGACATCAACGCCTGATGCGCAGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt < 1:484310/228‑1 (MQ=11) gccaccGCCAGGCAGGGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 1:164497/223‑1 (MQ=255) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 1:346087/227‑1 (MQ=18) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATACGCAGCTTGCCGCGTCTTATCAGGCCTatc > 1:399114/1‑226 (MQ=12) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGAATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTTTCAGGCCTACg > 1:342401/1‑228 (MQ=21) agatgcgcAATTGCCTGATTTTGGGGGATTTTGGAGGTCGGATAAGGCTGTTATGCCGCATCCGACACCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa < 1:68117/224‑1 (MQ=11) ggcgcAATAGCCTGAGTTAGCGTGATTTTGGAGGTCTGAAAAGGCGTTTAGGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAGGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACa < 1:214451/226‑1 (MQ=255) ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgc > 1:230552/1‑193 (MQ=34) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg < 2:230552/193‑1 (MQ=34) aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCAAAAGCCTGATTTTGCGTGAt > 1:403015/1‑228 (MQ=21) cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCCTCGCAATAACTTTTTTTAACGGCATTTTGtgtttcg > 2:596102/1‑222 (MQ=11) cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg > 2:125538/1‑179 (MQ=21) cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg > 1:366905/1‑169 (MQ=35) cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCACGACAACGAAATAGCCTGATTTAGCGTGATTTTGTTGGtgc > 1:619717/1‑226 (MQ=255) ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 1:125538/179‑1 (MQ=21) ggATAAGGCGTTTATGCCGCATCCGACATCAACGCATGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 2:366905/169‑1 (MQ=25) aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACGTCAATGCCTGATGCGACGCTTGCCGCGCCTTACCAGGCCTACCCCAGCAAGGGCAAACGCCTCATTTAGCTTAATTTGGtcgaccgga > 2:522489/1‑219 (MQ=11) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt < 2:142947/148‑1 (MQ=25) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt > 1:142947/1‑148 (MQ=37) cggcATCAACGCCGGATGCGACGTTTAACGCGTCTTAGCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTT‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg < 1:32237/225‑1 (MQ=255) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat < 1:26733/147‑2 (MQ=21) tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat > 2:26733/1‑146 (MQ=37) aTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGACTCCGCCATCAACACCTGa > 1:241933/1‑227 (MQ=255) gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc < 2:61694/126‑1 (MQ=25) gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc > 1:61694/1‑126 (MQ=38) tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc > 2:281479/1‑103 (MQ=32) gcTTATCAGGCCTAGACCAGGCAGCGTAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGC‑Gt < 1:30101/226‑1 (MQ=19) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACg < 1:281479/103‑1 (MQ=32) gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATCGCCTGATTTCGCGTGATTTTGTGTGTCGGATAAGGCGTTTACGCCGCATCCGTCCTCAACACCTGCTGCGCCCCTTCACGC‑GTCTTTTCAgtc > 2:519142/1‑226 (MQ=11) ctcttgacaaGACAGCCCAATAGCCTTATTTAGCGTG‑AGTTTGTAGGTCGGATAAGGCGGTTACGCCGGATCCGACATCAACGCCTGATGCGGCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGC‑GTCTTATCAGGc < 1:161651/219‑1 (MQ=11) gttaagtcggACAGCGCAATAGCTTGATTT‑GGGTGTATTGTGTGGGACGGATAAGGCGTTTAAGCCGGGTACGACATCAACGCCGGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGC‑GTCTTATCAGGc < 2:288570/218‑1 (MQ=11) cggcAGGCAGAGCAATAGCCTGATTTAGCGTG‑ATTTTGGAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGC‑GTCTTATCAGGCCTACg < 1:193671/225‑1 (MQ=255) cGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGGCGGATAAGCCGTTTATGCCGCATCCGCCCTCAACGCCGAGTCCGACGCTTACCGC‑ATCTTACCAGCACTACg > 1:462237/1‑228 (MQ=11) cGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt < 1:74792/156‑1 (MQ=35) aCAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGAATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTCGCGCGTTCTTGTCGGTCGGCTAAGGCGCTTATGCCGCATCCGACTTCAACGCCCAATGAGACGCTTTACCA‑CTCTATTCACGCCTACACCacgc > 2:161651/1‑225 (MQ=11) cgcAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc < 2:307593/85‑1 (MQ=37) cgcAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTACGCCAGCCATCGCAATATCCTGATTTCGCGTCATTTTGTAGGTCGGATAAGGCGTTTATGCCCCATCCGACATCAACGTCTGATGCGACGCTT‑ACGCTGCCTTACCGGGCCAACGCCAGAcccgc > 2:297567/1‑224 (MQ=11) cgcAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc > 1:307593/1‑85 (MQ=37) aTTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCAGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGAATCCGACATCAACGCCTGATGCGACGCTTAACGC‑GCCTACTCAGGCCTACGCCAGCCAGCGCAATAGCCTGAt > 1:275609/1‑228 (MQ=11) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc < 1:629770/63‑1 (MQ=255) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc > 2:629770/1‑63 (MQ=39) cGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcg > 2:414895/1‑54 (MQ=255) ggATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc < 1:414895/54‑1 (MQ=255) tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGC‑GTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTACCGGTATTTTTCAAGTCGGATAAgggatt > 2:390828/1‑224 (MQ=11) tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGC‑GTCTTATCAGGCCTACGCCAGACAGCGCAATACCCTGATTTAGCGGGATTTTGTAGGTCGACAAAGGCGATTa > 2:514447/1‑228 (MQ=11) ttaTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCCATCAACGCCTGATGCGACGCTTACCGC‑GTCTTATCAGGCCTACTCCAGACAGCGCAAAAGCTCGTATTAGCGTGATTTTGCTGGTCGGATCAGGCCTTTaacccca > 2:242908/3‑222 (MQ=11) | ATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGC‑GTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGC > NC_000913/4295840‑4296273 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |