Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,260,629	T→C	20.2%	E261G (GAG→GGG)	fruA ←	fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,260,629	0	T	C	20.2%	98.4 / 2.2	49	E261G (GAG→GGG)	fruA	fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base T (18/18);  new base C (8/3);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTTCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGT  >  NC_000913/2260394‑2260858                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       cAGTTTCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGACCCC‑GGCCCtt                                                                                                                                                                                                                                     >  1:403212/1‑239 (MQ=255)    tttCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTCCCGGCACCA‑TCACCGCCAAGGCTGAACCACCACCAACCCGCCTCAGCGCCCCAACCCACGAACCC‑GGCACTTaaa                                                                                                                                                                                                                                  >  2:475433/1‑239 (MQ=255)    tttCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCAACAGCGCCGCAGCCAACGTACCC‑GGCCCTTTaa                                                                                                                                                                                                                                  >  1:518030/1‑239 (MQ=255)    tttCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGAACCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAAACTGCATCAGCCCCGCAGCCAACGTAACC‑GGCCCTTTaa                                                                                                                                                                                                                                  >  1:571848/1‑239 (MQ=255)      tCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCCGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCCGCCAACGTACCC‑GGCCCTTTaaa                                                                                                                                                                                                                                 >  1:392746/1‑238 (MQ=255)      tCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCCCCA‑TCAGAGCAAAGGCTGAACCACCACCAAACCGCACCAGCGCCGCAACCAACGAACCCTGGCCCTTaaaa                                                                                                                                                                                                                                 >  2:378891/1‑239 (MQ=255)                   gATTAACTTCG‑AATGTTAACCAGCCAGGAACCCCGAAATAATCCCACC‑AATGAAGCCAAACCCGGTGCTGCCCGCCAGCATCCCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACcaa                                                                                                                                                                                                                   <  1:254525/239‑1 (MQ=255)                    aTTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCCGCGCCGCGGCCAACGTACAC‑AGCCCTTTAAACACTTCGATACcaaa                                                                                                                                                                                                                  >  1:645469/1‑239 (MQ=255)                              cAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACAACCACCAATCTGCATCAGCGCCGCAGCCACCGTACCC‑GGCCCTTTAAACCCTTCGATACCAAAAACAaaagaa                                                                                                                                                                                                        >  1:771460/1‑239 (MQ=255)                                     acccagccagAAACCCCGAAATAAT‑CCACCAAAAGAAGCCGAAACCGGTGCGGACCGCCAGCATGCCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGcgcg                                                                                                                                                                                                  <  1:659487/237‑1 (MQ=255)                                            cagGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCGGCCAACGTACCC‑GGCCCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGGGCGATAcaca                                                                                                                                                                                           >  1:112861/1‑239 (MQ=255)                                              ggAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAACCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGCTACCAAAAGCAAAACAAAGCCCGAAAaacaa                                                                                                                                                                                          >  2:263829/1‑237 (MQ=255)                                                     cGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGGAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCCATACCAAAAGCAAAGGAAAGCGCGATACACAGACCACCt                                                                                                                                                                                   >  1:489868/1‑238 (MQ=255)                                                             tcccccc‑aATGAACCCAGTACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGAcc                                                                                                                                                                          <  1:697019/234‑1 (MQ=255)                                                                  cc‑aATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAAAAAACGCGAACAACAAACCACCGGCAAAGACCACCgg                                                                                                                                                                     >  2:746006/1‑239 (MQ=255)                                                                      aggaccccAGAACCGGTGCGCACCCCCAGAATACCGCCAAGCAGCCCCGGAGTGGGG‑CCCGGACGA‑CCGGAAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCCCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGgcag                                                                                                                                                                  <  2:370129/233‑1 (MQ=255)                                                                       tGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGgcagc                                                                                                                                                                 >  1:370129/1‑239 (MQ=255)                                                                              aGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAACAGAAAGCGCGATACCACGCACACCTGCACAGACCATCGGCAGCACGTAAg                                                                                                                                                          >  1:59937/1‑239 (MQ=255)                                                                                  cggggggaacccGCCAACATGCCACCAACCAGCCCCGGAGGGGGG‑CCCGGACGA‑TGGGCAAGGGAAAAGGCAATATAC‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGaaa                                                                                                                                                       <  2:42671/228‑1 (MQ=255)                                                                                     gTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAACGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGTTACCAAAAGCAAAAGAAATCGCGATACCAAAACACCCTGCAACGACCATCGGCAGCATGTAAGAAACGc                                                                                                                                                    >  1:374084/1‑238 (MQ=255)                                                                                                  gattACCGCCATTCAGTCCCGGTGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGACAATTTAC‑CCTGCCAGTACCGGCACCG‑TCAGCGCAAAGGCTGACCCTCCACCAATCTGCACCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                                                      <  2:112861/236‑1 (MQ=255)                                                                                                  cccaccccccAATCAGACCCGGGTTGAGG‑CCCGGACGA‑TCGGCAAGGGAAAAGGCAAGATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                                                      <  2:426340/231‑1 (MQ=255)                                                                                                    aTACCGCCAATCAGACCCGGATTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGAc                                                                                                                                     <  1:402090/238‑1 (MQ=255)                                                                                                       cccccAACCAGACCCGG‑GTGGGGCCCCGGACGACTCGG‑ATGGGAAAAGGCAATATAG‑CCTGCCAGTACCGGCCCCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTa                                                                                                                                 <  2:374084/236‑1 (MQ=255)                                                                                                       ccGCCAACCAGGCCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTa                                                                                                                                 <  1:387094/239‑1 (MQ=255)                                                                                                          ccAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATg                                                                                                                               >  1:651044/1‑238 (MQ=255)                                                                                                            aaacAGGCCCGGAGTTAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATgcgc                                                                                                                            <  1:197266/236‑1 (MQ=255)                                                                                                                   cccGGTGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATAAAA‑CCTGCCAGTACCGGCACCA‑GCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCGCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCa                                                                                                                       <  1:710087/237‑1 (MQ=255)                                                                                                                   cccGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCAct                                                                                                                     <  1:475433/239‑1 (MQ=255)                                                                                                                   ccaggggTGAGG‑CCCGGCCGA‑ACGGAAGGAGAAAAGGAAATAAGA‑CCTGCCAGTTCCGGCACCA‑TCAGAGCAAAGGCCAAACCCCCACCAAGCTGCAACAGCGCCTCAGCCAACCTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGc                                                                                                                        <  2:630539/230‑1 (MQ=255)                                                                                                                     cggggggAGG‑CCCGGGAGA‑ACGGGAATGGAAAAAGGAAAATAACCCGGCCAGT‑CCGGTCCCA‑ATAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCAct                                                                                                                     <  1:528602/231‑1 (MQ=255)                                                                                                                                   ggACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTc                                                                                                      >  1:706492/1‑239 (MQ=255)                                                                                                                                   ggACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGC‑AAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGCAAGCGCCTGCCCTCTCTTTCTTACtttac                                                                                                     >  1:699113/1‑237 (MQ=255)                                                                                                                                          tCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGCCGGTATGCGCCTGCACTCCCTTTCTTACTTTCAGTgg                                                                                                 >  1:245827/1‑238 (MQ=255)                                                                                                                                           cGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTc                                                                                               <  1:104809/239‑1 (MQ=255)                                                                                                                                           cGGCAATGGAAAAGAAAAAAAAA‑CCTGCCAGTACCGGCACCA‑TCACCGAAAAGTCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCTTGCACTCTCTTTCTTACTTTCAGTGGTc                                                                                               <  2:874074/239‑1 (MQ=255)                                                                                                                                                     aaaGGCAATACAA‑CCTGCCCGTACCGGCCCCACTCGGCCCAAGGCCTAAACC‑CCCCCAAACTGCATCAGCGCCGCACCCAACGTACCC‑GGCCCTTTAAACGCTACGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCCCCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAg                                                                                    <  2:245827/239‑1 (MQ=255)                                                                                                                                                              ataC‑CCTGCCAGTACCGGCACCA‑TCAGCCAAAAGGCTGACCCACCCCCAATCGGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATCCCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAGCTTTGCccg                                                                           <  1:284756/239‑1 (MQ=255)                                                                                                                                                                      gCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAGCTTTGCCCCCCGGTTc                                                                    >  1:716332/1‑239 (MQ=255)                                                                                                                                                                      gCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GCTTAAGCTTTGCCCGCCGGTTc                                                                    >  1:604555/1‑239 (MQ=255)                                                                                                                                                                      gCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGG‑CGCCAGTTTGAGCTTTGCCCGCCGGTTc                                                                    >  1:910352/1‑239 (MQ=255)                                                                                                                                                                        cAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGGGGTCGCC‑GTTTGAGATTTGCCCGCCGGTTCAt                                                                  >  1:823791/1‑239 (MQ=255)                                                                                                                                                                                                aGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCt                                           >  1:660212/1‑239 (MQ=255)                                                                                                                                                                                                            caccaATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATccctgtct                               >  1:649026/1‑233 (MQ=255)                                                                                                                                                                                                                cttCTGCATCAGCCCCGCAGCAAACGGCCCC‑GGCTTTTTAAACGCTTCGAAACCAAAAAAAAAAAAAACCCCGATACACAGACCCCCTGCACGGACCACGGGCAAAATGAAAGAACCGCCCGTCAGCAAGTGCCGGTAGGCGCCTGCCCTCTTTTTCTTCCTTTCAGTGGTCGCC‑GTTTGATCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTgcg                           <  2:651044/237‑1 (MQ=255)                                                                                                                                                                                                                    tGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGAATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGcccg                         >  1:346206/1‑237 (MQ=255)                                                                                                                                                                                                                                 cAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCaa                                                                                                                                                                               >  1:571451/1‑75 (MQ=255)                                                                                                                                                                                                                                 cAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCaa                                                                                                                                                                               <  2:571451/75‑1 (MQ=255)                                                                                                                                                                                                                                         gTACCC‑GGCCCTTTAAACGCTTCGATACCAAAAGAAAAAAAAAGCCGGATACACAGACCACCTGCACCGCCCATCGGCAGCATGTAAGAACCGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGGGGTCGCC‑GTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGt  <  1:263829/239‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       CAGTTTCAATTGCGTACTGATTAACTTCGCAATG‑TAACCAGCCAGGAAGCCCGCAATAATACCACC‑AATGAAGCCAGAACCGGTGCTGACCGCCAGCATACCGCCAATCAGACCCGGAGTGAGG‑CCCGGACGA‑TCGGCAATGGAAAAGGCAATATAA‑CCTGCCAGTACCGGCACCA‑TCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCC‑GGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCC‑GTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCTGCGCGGTTTTCTTCAGCGCCAGACCGGT  >  NC_000913/2260394‑2260858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑