Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,300,864	T→C	14.9%	E138G (GAG→GGG)	yhbT ←	SCP‑2 sterol transfer family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,300,864	0	T	C	14.9%	164.3 / 2.4	72	E138G (GAG→GGG)	yhbT	SCP‑2 sterol transfer family protein
Reads supporting (aligned to +/- strand):  ref base T (43/16);  new base C (3/9);  total (46/26)
Fisher's exact test for biased strand distribution p-value = 2.70e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACGCACATGAA  >  NC_000913/3300630‑3301098                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            ccccAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAg                                                                                                                                                                                                                                         >  1:543792/1‑239 (MQ=255)                ccctctttCCCGCAGTCCGTGACCCACCTTCCCTCCCGCTTCCGTTACGAAGGGGCGACGAAGCGGGGCAAGAATACCCGTCGCATC‑ATGGGAATTTTCTCCTGGATTTAGCAGGTTTCACCTACCGATG‑GTGTTTTGTTTGCAGGCGCGGTTTTC‑T‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCAt                                                                                                                                                                                                                          <  2:915434/233‑1 (MQ=255)                    tCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑AAGGCGCGGTTTTC‑A‑TTCCCGCCTCACCAAAATCCGCCAGTTGGAGGACCATCTTCCGTAAAGCTTTCGGAATTTGCCCCCGGTCAATgggcgaccacagg                                                                                                                                                                                                                      >  2:808317/1‑228 (MQ=255)                       tCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCACCATCATGCGTAAGGCTTTCGCCATTTGTCCCAGTTCAATGGCGTCCATCAGGttt                                                                                                                                                                                                                   >  1:161686/1‑239 (MQ=255)                               aTCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACAt                                                                                                                                                                                                            >  1:684049/1‑238 (MQ=255)                                tCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGGTTTTCACATAc                                                                                                                                                                                                          >  1:363070/1‑239 (MQ=255)                                         aGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCCACAAAATCCGCCCGTTGCAGCAGCATCCTTCGTACGGCTTTCGGCATTTGCTCCAGTTCCATTGGGTCCATCCGGTTTTTCCACTACAGccccca                                                                                                                                                                                                  >  2:857284/1‑236 (MQ=255)                                         aGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCCTTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGcccccg                                                                                                                                                                                                  >  1:856666/1‑236 (MQ=255)                                         aGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATCGGTAAGGCTTTTGGCATTTCCTCCAGTTCAATGGCGTCCACCGGGTTTTTCACATAAAGCCCCac                                                                                                                                                                                                  >  2:650746/1‑237 (MQ=255)                                           cttcgcttcCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATCAAGCCCCAGCt                                                                                                                                                                                                >  1:731194/1‑238 (MQ=255)                                           cttcgcttcCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTACCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATTCGTAAGGCTTTCGGCATTTGCTCCAGTTCACTGGCGTCCATACGGATTTTCACATACAGCCCCAGCt                                                                                                                                                                                                >  1:729562/1‑238 (MQ=255)                                                              tCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCCCATACAGCCCCAGCTCCGGATCGCCTTCaa                                                                                                                                                                                 >  1:614018/1‑234 (MQ=255)                                                                  aTGGGCGAGGCAGCAGGGCATCAATCCCCGGCGCATCAATGGGAT‑TTTCTACCCAAATTAGCATGGTTCCCCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGcc                                                                                                                                                                        <  1:77496/239‑1 (MQ=255)                                                                     aGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGAGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGa                                                                                                                                                                      >  1:734278/1‑238 (MQ=255)                                                                                       cAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCCGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACTTACAGCCCCCGCTCCGTATCGCCTTCAATAACCCGCCGCCGCTGGAAAAAAAGCATa                                                                                                                                                    >  2:207820/1‑238 (MQ=255)                                                                                       cAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCgaa                                                                                                                                                    >  1:454600/1‑236 (MQ=255)                                                                                       cAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGGTTTTCACATACAGCCCCAGCTCCGGATCGCCTTCAATCACCAGCCGACGCTGGAAGGAGAGCGTa                                                                                                                                                    >  1:782529/1‑238 (MQ=255)                                                                                       cAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGCTTTT‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCATTTCAATGGCGTCCATCAGGTTTTTCACATACATCCCCACCTCCGTATCTCCTTCAATCACCACCCGACCCTGGAAGCAAAGCGTa                                                                                                                                                    >  1:132241/1‑238 (MQ=255)                                                                                         aTACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTACGGCTTTCGGCATTTGGTCCAGTTCAATGGCGTCCATCAGGTTTTTCCCATCAAGCCCCCGCTCCGTATCGCCTTCAATCCCCAGCCGACCCTGCAAGACAAGCGTATcc                                                                                                                                                 >  1:559647/1‑239 (MQ=255)                                                                                         aTACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATcc                                                                                                                                                 >  1:885922/1‑239 (MQ=255)                                                                                            cccGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAACCACCAGCCGCCCCTGGAAGAAGAGCGGATCCg                                                                                                                                                >  2:784622/1‑237 (MQ=255)                                                                                            cccGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATACGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGa                                                                                                                                              >  1:687750/1‑239 (MQ=255)                                                                                               atcccATCAATGTGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCCTCCGTAAGGCTTTCGCCATTTGCTCCAGTTCAATGGCAGCCATCAGGTTTTTCCAATACAGCCCCAGCTCCCGATCGCCTTCAATCCCCCACCGACACCGGAAAAAGAGCGTATCCGGa                                                                                                                                              >  2:766419/5‑236 (MQ=255)                                                                                                 cgcATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCCGCTCCGTCTCGCCTTCAATCACCAGCCGACGCTGGAAGAAAAGGGAATCCGGAACTTg                                                                                                                                         >  2:57814/1‑239 (MQ=255)                                                                                                 cgcATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGCCATTTGCCCCCGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCACCTCCCTATCGCCTTCAATCCCCAGCCGACCCTGGAAGACGAGCGAAGCCGCACCttt                                                                                                                                         >  2:441566/1‑238 (MQ=255)                                                                                                    aTCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGttt                                                                                                                                      >  1:168940/1‑239 (MQ=255)                                                                                                       aaTGTGAT‑TTTCCACTCGAATTTCAATGTTTCCCCTTACG‑TGTGTTGTTGGTTT‑CAGGGCGGGTTTGC‑ACTCCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTAcg                                                                                                                                   <  2:731194/239‑1 (MQ=255)                                                                                                              attttCTACCCGAATCGGCTGGTTTCCCCTACGTATTGTTGTTGGGTTT‑CAGGGGGGTTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGcgccg                                                                                                                              <  1:857284/236‑1 (MQ=255)                                                                                                                           aTTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGACCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCACCCGCCCCTGGAAGACGAGGGTATCCGGATCTTTGTTAAGCGCCGCAataaccaagaaa                                                                                                                >  2:53743/1‑229 (MQ=255)                                                                                                                                     ttCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCCTcag                                                                                                                    >  1:100075/1‑225 (MQ=255)                                                                                                                                     ttCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTggga                                                                                                       >  1:541738/1‑236 (MQ=255)                                                                                                                                     ttCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCACCTCCGTATCCCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGCATCTTGTTTACGCCCCCCAATCATCCCAAGATCCCTGGCgc                                                                                                      >  2:141305/1‑238 (MQ=255)                                                                                                                                     ttCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGGTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCCCCAGCCGACGCTGGAAAAATACCGAATCCGGACCTTGTTTACCCCCCCCAATCATAAGCAAAACGCTGGCg                                                                                                       >  2:634111/1‑238 (MQ=255)                                                                                                                                          cTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCCCTGGCGTCg‑gac                                                                                                >  1:325249/1‑237 (MQ=255)                                                                                                                                             ccGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTCTCGCCTTCAATCACCACCCGACGCTGGACGAAGACCGTATCCGGTTCTTGTTTACCGACCGCAACTATCAGCAGATCACCGGCGTCT‑GCCCTa                                                                                             >  1:877307/1‑239 (MQ=255)                                                                                                                                               gATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTaaa                                                                                           >  1:38378/1‑239 (MQ=255)                                                                                                                                               gATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTaaa                                                                                           >  1:312186/1‑239 (MQ=255)                                                                                                                                                  ttttgttttgttt‑CAGGGGTGTTTTTCTC‑CCCCCGCCTCAAAAAAATCCGCCAGTTGCAGCAGCTCCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATCCAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAAc                                                                                         <  2:168940/236‑1 (MQ=255)                                                                                                                                                    cTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTTGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAGGAAGAGCGTATCCGGACCTTGTTTACGCCCCGCAATCATCAGCAGATCGCCGGCGTCG‑GCACAAAAACCca                                                                                      >  2:268215/1‑239 (MQ=255)                                                                                                                                                       tttGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGCCGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCCTCG‑CCACTAAAAACCACAt                                                                                   >  2:218490/1‑239 (MQ=255)                                                                                                                                                                                ttccccGCCTCAACAAAATCCGCCAGTTGCAGCAGCCTCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAAc                                                             <  2:684049/236‑1 (MQ=255)                                                                                                                                                                                     cGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGCCCa                                                        >  1:496601/1‑239 (MQ=255)                                                                                                                                                                                     cGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGCCTAACGACCa                                                        >  1:690759/1‑239 (MQ=255)                                                                                                                                                                                          caacaaAATCCCCCAGGTCCACCAGCATCAAGCTTAATGCTTTCGGCATTTCCCCCAGTTCAATGGCGTCCATCGGGTTTTTCACATACAGCCCCAGCGCCGTATCGCCTTCAATCCCCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTg                                                   <  2:194734/239‑1 (MQ=255)                                                                                                                                                                                           aacaaAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGTTTTTTCACATACAGCCCCAGCTCCGTCTCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGAATCCGCATCTTGTTACCCAGCCGCAATCAACAGCCGATCGCTGCCGTCGCGCA‑TAAAACCGCCATCAGCTTTGCCGTTTTGACTACCGACCAgagtgc                                                  >  2:145784/1‑234 (MQ=255)                                                                                                                                                                                               aaaCCCCCCAGTTGCAGCCGCCTTATGGGTAAGGTTTTCGGCATTTGCCCCAGTTCAATGGCGTCCATCAGGTTTTCCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCAtt                                              <  2:431678/239‑1 (MQ=255)                                                                                                                                                                                                     gCCCGTTTCCGCAGCCTCAGTCTTAAGGCTTTCGGCCTTTGCCCCAGTTCCATGGCGTCCTTCAGGTTTTTCCCATACAGCCCCAGCTCCGGATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTcaccac                                        <  2:782529/239‑1 (MQ=255)                                                                                                                                                                                                     gCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCTTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACTCCCCGCAATCATCAGCAGATCGCTGGCCTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTTCGGCTAACACCCAGTTTGCCATTcacac                                         >  1:840067/1‑236 (MQ=255)                                                                                                                                                                                                     gCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTcaccac                                        <  1:214275/239‑1 (MQ=255)                                                                                                                                                                                                     cccAGTTGCCGACGCATCTTGTGTAAGGTTTCCGGCATTTTCTTCATTTTCATGGCGTCCAGCCGGTTTTGCACATACAGCCCCAGCTCCGGATCGCCTTCAATCGCCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGGCCAGTTTGCCATGcaccac                                        <  2:411781/238‑1 (MQ=255)                                                                                                                                                                                                          ttGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGACGATCGCTGGCGCCG‑GCACTAAAACTCACATCAGCTTCCCCGTTTTGGCTAAGGACCAGTTTGCCATTAACAACCCAgg                                   >  2:642391/1‑239 (MQ=255)                                                                                                                                                                                                           tGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAgg                                   <  1:141305/238‑1 (MQ=255)                                                                                                                                                                                                                  gcaTCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCAt                           <  1:881936/239‑1 (MQ=255)                                                                                                                                                                                                                   catcatGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTcaccac                                        <  1:801805/225‑1 (MQ=255)                                                                                                                                                                                                                   catcatGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTcac                                           <  1:348971/222‑1 (MQ=255)                                                                                                                                                                                                                    ttcttGCTTAAGGCTTTCGGCTTTTGGCCCATTTCAATGGCTTCCATCAGGTTTTTCACATGCAGCCCCAGTCCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTg                         <  2:541738/235‑1 (MQ=255)                                                                                                                                                                                                                    ctcatGTGTGAGGTTTTCTGCTTTTGTCCCATTTCAAATGCGTCCATCCGTTTTTTCACATTCAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCATTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTg                         <  2:363070/238‑1 (MQ=255)                                                                                                                                                                                                                     tcagGCGGAAGGCTTTCGGCATTTGCCCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTg                         <  1:784622/238‑1 (MQ=255)                                                                                                                                                                                                                         cctgagggCTTTCGCCCTTTCCCCCACTTCAGTGGCGTCAATAAGTTTTTTCACATGCAGCCCCAGCCCCGTACCCCCTTAAATCCCCATCCGCGGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGt                    <  2:914301/233‑1 (MQ=255)                                                                                                                                                                                                                          cGTTAGGCTTTCGGCTTTTGCCCCCGTTCAATGGCGTCCATCAGGTTTTTCACATACCGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGt                    <  2:423823/238‑1 (MQ=255)                                                                                                                                                                                                                            tAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGAAAACCATTGAAGGCCaa                 >  1:848717/1‑239 (MQ=255)                                                                                                                                                                                                                                  ttttGGCATTTGCTCCAGTTCAATGGCCTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCa            <  1:268215/238‑1 (MQ=255)                                                                                                                                                                                                                                   ttCGGGCTTTTCTCCAGTTGAATGGCTTCCATCAGGTTTTTCACATGCAGCCCCAGCTCCGTTTCGCCTTCAATCCCCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACg          <  2:496601/239‑1 (MQ=255)                                                                                                                                                                                                                                   ttCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACg          <  1:665758/239‑1 (MQ=255)                                                                                                                                                                                                                                   ttCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCACCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGCAAACCATTGCAGGGCaaaaacaac          >  2:469354/1‑232 (MQ=255)                                                                                                                                                                                                                                   ttCGGCATTTGCTCCAGTTCAATGGCGGCCATCAGGTTTTGCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGCCGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACg          <  1:351636/239‑1 (MQ=255)                                                                                                                                                                                                                                   ttCGGCATTTGCCCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACg          <  1:650746/239‑1 (MQ=255)                                                                                                                                                                                                                                    tCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCCCTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGCCCAGTTTGCCATTCACCACCGCGGGAACCAATGTCAGGCCAACATccccc         >  2:143775/1‑235 (MQ=255)                                                                                                                                                                                                                                    tCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACg          >  1:423823/1‑238 (MQ=255)                                                                                                                                                                                                                                    tCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTACAGGTCAAAACCACGc         >  2:665758/1‑239 (MQ=255)                                                                                                                                                                                                                                         aTTTGCGCCAGTTCAATGGCCTCCATCAGGTTTGTCACATACAGCCCCAGTTCCGTATCGCCTTCAATCCCCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACGCACATg    <  1:886492/239‑1 (MQ=255)                                                                                                                                                                                                                                           ttGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCCCTAAAACTCACATCAGCATTCGCGTTCTGGCTAACGACCCGTTTGCCATTCACCACCCAGGTAAACCATTGCAGGGCACAAACACGCAAATGaa  >  2:484265/1‑239 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            CCCCAGCGTCAGAAAGCCATCTTCACGCAGATCGTGAACCAGCTTCGCTTCCGCATCACTTTCGAATGAGCGACGCAGCAGGGCATCAATACCCGGCGCATCAATGGGAA‑TTTCTACTCGAATTAGCATGGTTCACCTACCGATGTCTGTTTGGTTT‑CAGGCGCGGTTTTC‑A‑TTCCCGCCTCAACAAAATCCGCCAGTTGCAGCAGCATCATGCGTAAGGCTTTCGGCATTTGCTCCAGTTCAATGGCGTCCATCAGGTTTTTCACATACAGCCCCAGCTCCGTATCGCCTTCAATCACCAGCCGACGCTGGAAGAAGAGCGTATCCGGATCTTGTTTACGCGCCGCAATCATCAGCAGATCGCTGGCGTCG‑GCACTAAAACTCACATCAGCTTGCGCGTTCTGGCTAACGACCAGTTTGCCATTCACCACCGAGGTAAACCATTGCAGGTCAATATCACGCACATGAA  >  NC_000913/3300630‑3301098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑