Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,268 T→C 11.9% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC11.9% 75.8 / 3.6 41intergenic (+474/+168)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (19/15);  new base C (2/3);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

TACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCAAACCCTTCCATACAGCTCAGGTTC  >  NC_000913/4296040‑4296502
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             
tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTACCCCGCATCCg                                                                                                                                                                                                                                     >  1:379653/1‑239 (MQ=25)
 accccgcATGCGACATCAACGCCTGATGCGACGCTTGGCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATACCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATGCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTACGCCGCATCCGa                                                                                                                                                                                                                                    <  1:55169/236‑1 (MQ=255)
 aCGCCCAATCCGAGATTAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTACGCCGCATCCGa                                                                                                                                                                                                                                    <  1:76893/239‑1 (MQ=12)
     cgcATCCGGAATCAACGCCTGATGCGACGCGTGCCGAGTCTTATCAGGCCTACGCCATACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTACGCCGCATCCGACATc                                                                                                                                                                                                                                <  1:230862/239‑1 (MQ=255)
     cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGGAACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAACGCAAAAGCCTGCTTTACCTGGATTTTTTAGGACGGAGAAGGA‑GTTAACGccgacaccgacaac                                                                                                                                                                                                                                >  2:298567/1‑237 (MQ=11)
      gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCCCACTAGCATGATTAAGCGGGATTTTGTAGTTCGGATAAGGC‑GTTTAGGCCGCACCCGAAATCa                                                                                                                                                                                                                               >  2:454676/1‑239 (MQ=11)
                           tGCGACGCTTGCCGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                           <  1:545535/238‑1 (MQ=17)
                                                                                                                                                                          cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTAATCAGGCCTATCTTAACCGTTgg                                                           >  1:548297/1‑239 (MQ=12)
                                                                                                                                                                               gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAAACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCGTCCGACACAAAGCCCTGTTGTGACCCTTGCCCCGTCGTCAAAGGCCTATCTTAACCTTTGGTAAAt                                                      >  2:819363/1‑239 (MQ=11)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCATTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGCCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAACCTTCCCCCGCCTTATCAAGCCTACCTTAACAGTGGGTTAATTTTTc                                                 >  2:112503/1‑239 (MQ=11)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTACCCGTTGGTTAATTTTTc                                                 >  1:179807/1‑239 (MQ=255)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGCCTTATCAGGCTTATCTTAACCGTTGGTTAATTTTTc                                                 >  1:195512/1‑239 (MQ=255)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATCCGACGCTTGCCCCGTCTTATCAGGCCTATCTTAACCTTGGGTTAAtttttt                                                 >  2:903686/1‑238 (MQ=255)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATCCCTGATGCGCCGCTTCCCGCTTCTTATCAGCCCTATTTTAACCTTTGGTTAATTTTTc                                                 >  2:568936/1‑239 (MQ=11)
                                                                                                                                                                                    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACCAATGCCTGATGGGACGCTTGCCGCGTCTTATAAGGCCCATCTTAACCTTTGGTTAATTTTTc                                                 >  1:777636/1‑239 (MQ=11)
                                                                                                                                                                                     ggaaTTGCCTGATTTAGCGGTATTTTGGAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                      <  1:212127/231‑1 (MQ=11)
                                                                                                                                                                                        attggcTGATTTAGCGTGATTTTGGAGGTCGGATAAGGC‑GTTTAAGCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                             <  1:298567/234‑1 (MQ=14)
                                                                                                                                                                                        aTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  1:704939/238‑1 (MQ=255)
                                                                                                                                                                                         tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCTTGGGTTAATTTTTCaaa                                              >  1:13765/1‑237 (MQ=255)
                                                                                                                                                                                              tGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTAGTTCGGATCAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCCTCTTATCAAGCCTATCTTAACCGTTGGTTAATTTTTCAAACCCTTc                                        >  2:180262/1‑238 (MQ=14)
                                                                                                                                                                                                 tttAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAATTACTGATGCGACGCTGGCCGCGTCTTATCAGGCCTATCTTAACCGTAGGTTAATTTTTAAACCCTTTcaca                                     >  2:541886/1‑238 (MQ=11)
                                                                                                                                                                                                  ttAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑cc                                  <  1:257928/239‑1 (MQ=255)
                                                                                                                                                                                                   ttagggttttttGTTGGTGGGGTATGGGGGTTTCTGCACCATCCGCCATAAACGCCTGATGGGACGGTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑cc                                  <  2:777373/231‑1 (MQ=14)
                                                                                                                                                                                                     gCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAATGCCTGATGCACCGCTTGCCCCGTCTTATCAGGCCAACCTTAACCGTTGGTTAATTTTTAAAACACTTCCAA‑CCCgg                               >  2:578344/1‑239 (MQ=11)
                                                                                                                                                                                                          aTTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAATGCCTGATGCGACGCTTGCCCCGTCTTTTCAGGCCTATCTTAACCGTTGGTTAATTTTTAAAACTCTTCACA‑CCCggaaaca                          >  2:347805/1‑234 (MQ=14)
                                                                                                                                                                                                            ttttgggggcGGATAAGGG‑GTTTTTTCCCCACCCGACACCAACGCCTGATGCGACGCTTAACGCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCaaa                        <  2:523971/230‑1 (MQ=17)
                                                                                                                                                                                                            tttGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCaat                        <  1:732763/239‑2 (MQ=255)
                                                                                                                                                                                                            tttGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCaaa                        <  1:454676/239‑1 (MQ=255)
                                                                                                                                                                                                            tttGTAGGTCGGATAAGGC‑GGTTATGCCGCATCCGACATGAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCaaa                        <  1:465199/239‑1 (MQ=255)
                                                                                                                                                                                                            tttGGAGGCCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCaaa                        <  1:541886/239‑1 (MQ=255)
                                                                                                                                                                                                                     cGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcaca                                     <  2:167856/218‑1 (MQ=255)
                                                                                                                                                                                                                     cGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcaca                                     >  1:167856/1‑218 (MQ=255)
                                                                                                                                                                                                                     cGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGAATCAAACCCTTCCaa               >  2:427069/1‑238 (MQ=255)
                                                                                                                                                                                                                           aGGT‑GTTTATGCCGGCTCCGACATCAACGCCTTAGCAGGCGCTTAACGCGTCTTTTCAGGCCTACGCCAGACAGCGCAATAGCTTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATTCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCAAACCCTTCCATACAGCt         <  1:865613/239‑1 (MQ=17)
                                                                                                                                                                                                                           aGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCAAACCCTTCCATACAGCt         <  1:578344/239‑1 (MQ=255)
                                                                                                                                                                                                                           aGGC‑GTTTATGCCGCATCCGACATCAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCAAACCCTTCCATACAGCt         <  1:112503/239‑1 (MQ=255)
                                                                                                                                                                                                                             gC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAACA‑CCCGGTATCAAACCCTTCCATACAGCTCa       >  1:763059/1‑239 (MQ=255)
                                                                                                                                                                                                                             gC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGCGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCATCAATGCCTGATGCGACGCTTTCCGCGCCTAATCAGGCCCATCTTAACCGTTGGTTAATTTTTCAAACCCTT‑ACACCCCGGTTTACAACCCTTCcacacaatcca       >  2:666386/1‑234 (MQ=14)
                                                                                                                                                                                                                                  ttATGCCCCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCAAACCCTTCCATACAGCTCAGGt    <  1:903686/238‑1 (MQ=255)
                                                                                                                                                                                                                                   tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCAA‑CCCGGTATCAAACCCTTCCATACAGCTCAGGTTc  >  1:125287/1‑239 (MQ=255)
                                                                                                                                                                                                                                   tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCAAACCCTTCCATACAGCTCAGGTTc  >  1:775059/1‑239 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                             
TACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGC‑GTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA‑CCCGGTATCAAACCCTTCCATACAGCTCAGGTTC  >  NC_000913/4296040‑4296502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑