Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT34.0% 78.4 / 69.3 96intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (36/26);  new base T (23/9);  total (60/36)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC  >  NC_000913/4295918‑4296202
                                                                                                                                              |                                                                                                                                              
tAAGGCGTTTATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                <  2:9287/143‑1 (MQ=17)
tAACGCGTTTCTGCCGCATCCGACATCATCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                <  2:1169847/143‑1 (MQ=12)
gaaGGCGTTTCTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                <  2:1055694/142‑1 (MQ=17)
   ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAGCGcc                                                                                                                                             >  1:758930/1‑143 (MQ=17)
   ggCGTATCTGCCCCGTCCGAGATCAACGCCTGATGCGACGCTTAACGCCTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTCAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGcc                                                                                                                                             <  2:1148797/143‑1 (MQ=11)
    gggTTTATGCCGCATCCGACACCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                            <  1:950771/141‑1 (MQ=17)
    gCGTTTATGCCGGATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                            <  1:1012994/143‑1 (MQ=17)
    gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt                                                                                                                                            <  1:876260/143‑1 (MQ=17)
    gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                            >  1:10886/1‑143 (MQ=32)
      gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa                                                                                                                                          >  1:719707/1‑143 (MQ=32)
      gTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTCGTAGGCCGGATAAGGCGGTCACGCCGCATCCGACATCAATGCCTGa                                                                                                                                          >  1:590265/1‑143 (MQ=11)
       tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGATTACGCGGCATCCGCCATCAATGcccgcg                                                                                                                                         >  1:1040201/1‑139 (MQ=11)
        ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCGTGACTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCCATGCCTGATg                                                                                                                                        >  2:706852/1‑143 (MQ=255)
           tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGa                                                                                                                                     >  2:790480/1‑143 (MQ=17)
            gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAGGCCTGATGCgcc                                                                                                                                    >  2:956933/1‑141 (MQ=12)
               gCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTGGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCt                                                                                                                                 <  1:311739/143‑1 (MQ=12)
               gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGCATAAGGCGTGTACGCCGCATCCGACATCCATGCGCGATGCGACggt                                                                                                                                 >  2:1407835/1‑141 (MQ=11)
                  tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                              >  1:523781/1‑143 (MQ=21)
                  tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGc                                                                                                                              >  1:1139972/1‑143 (MQ=34)
                   ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCGATGCCCTGTGCGACGCCTGcc                                                                                                                             >  2:498120/1‑143 (MQ=11)
                   ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                             >  2:890445/1‑143 (MQ=21)
                      aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGCCGCTTGCcgcg                                                                                                                          >  1:1218499/1‑143 (MQ=21)
                       cATCAACGCCTGATGCGACGCTTAACGCGTCTTTTCTGGTCTACCCCAGACAGCGCAATAGCCTGATTTAGCATGATTTTGTTCGTCGCATAACGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                         <  2:1139972/143‑1 (MQ=11)
                       cATCAACCCTTGATGCGACGCTTATCGCGTCTTATCAGGCCTACGCCAGCCAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                         <  2:1425559/143‑1 (MQ=11)
                        aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc                                                                                                                        <  1:338455/143‑1 (MQ=39)
                          cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                      <  2:271958/143‑1 (MQ=18)
                          cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTAGCCGCGTCtt                                                                                                                      >  2:1369092/1‑143 (MQ=21)
                           aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                     >  2:1064927/1‑143 (MQ=25)
                           aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                     >  1:49976/1‑143 (MQ=25)
                           aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                     >  2:385615/1‑143 (MQ=21)
                           aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                     >  1:1420735/1‑143 (MQ=25)
                            aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTcctct                                                                                                                    >  2:1414045/1‑139 (MQ=37)
                               ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                 >  1:972513/1‑143 (MQ=21)
                                cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                >  2:1405847/1‑143 (MQ=37)
                                 tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGAAAAGAAAACGACACCTTTTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                               >  1:328923/1‑143 (MQ=11)
                                  gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                              >  1:171575/1‑143 (MQ=21)
                                    tGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTGTGTGGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCTTa                                                                                                            >  1:206533/1‑143 (MQ=11)
                                     gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCtct                                                                                                           >  2:768973/1‑141 (MQ=255)
                                     gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGATTACGCCGCATCCGCCATCAATGCCTGATGCGACGCGCGCCGCGTCTCCTCAGGccccc                                                                                                           >  2:1057557/1‑140 (MQ=11)
                                      cGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCGTGAGGAGACGCTTGCCGCGTCGTATCAGGcccgtc                                                                                                          >  2:933404/1‑139 (MQ=11)
                                       gACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg                                                                                                                          >  1:52827/1‑126 (MQ=2)
                                       gACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgcg                                                                                                                          <  2:52827/126‑1 (MQ=38)
                                        ccGCTTCACGCGTCTTGTCAGCGCTACGCCAGCCAGCGCAATAGCCTGATTGCGCGTGATTTTGTAGGGCGGATCGGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGcc                                                                                                        <  2:188793/142‑1 (MQ=14)
                                        aCGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCACAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGGCGCTTGCCGCGTCTTATCAGGCCTACGcc                                                                                                        >  1:277029/1‑143 (MQ=255)
                                         cggttgtcgcgTCGTATCAGGCCTACGCCAGACAGCGCAATAGCCCGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                       <  2:271227/136‑1 (MQ=255)
                                         cgcatgccgcgTCTGCTCGGGCTTACGGCAGGGAAGGCAGTAGGCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                       <  2:1061865/136‑1 (MQ=11)
                                          gCTTAACGCGTCGTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAg                                                                                                      <  1:315387/143‑1 (MQ=255)
                                                 gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                                                               <  1:432912/143‑1 (MQ=39)
                                                  cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGgcgct                                                                                              >  2:524387/1‑142 (MQ=17)
                                                       tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATCAGGCGTTTACGCCGCGCCCGGCGTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                         <  2:307196/143‑1 (MQ=11)
                                                        aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                           >  1:544063/1‑124 (MQ=40)
                                                        aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                                                                           <  2:544063/124‑1 (MQ=40)
                                                         tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                     <  1:1015387/113‑1 (MQ=25)
                                                         tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                     >  2:1015387/1‑113 (MQ=39)
                                                            ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                                                                    <  1:993461/95‑1 (MQ=34)
                                                             gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGCCGGCGCTTTATCCTGaat                                                                                   >  2:1288414/1‑141 (MQ=11)
                                                                tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTACGCTAGCCGGCGGAATCGCCTGCttagt                                                                                >  2:1433276/1‑140 (MQ=11)
                                                                tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTGTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGGCAGCGCAATAGCCTGCTTTAg                                                                                >  1:1101145/1‑143 (MQ=255)
                                                                  cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                       >  2:740670/1‑86 (MQ=18)
                                                                  cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGc                                                                                                                                       <  1:740670/86‑1 (MQ=25)
                                                                     cAGACTGCGCAATGGCCTGCTGTAGCGTGATTTTGTAGGGCTGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa                                                                           <  2:198925/143‑1 (MQ=14)
                                                                     cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCGGCGGAATAGCCTGCTGTCGCGTGa                                                                           >  2:668452/1‑143 (MQ=14)
                                                                          aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCGACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCCTAGCCGGCGCTTTAGCCTGCTTTCCCGTGAttccg                                                                      >  2:792223/1‑140 (MQ=11)
                                                                          aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                           <  2:1207788/122‑1 (MQ=40)
                                                                          aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTGCAGCGTGAtctcc                                                                      >  2:188988/1‑139 (MQ=255)
                                                                            cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAAAAGCCTGATATATCGTCAttctcgc                                                                    >  2:664144/1‑138 (MQ=14)
                                                                              cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCGGCGCCATAGCCTGCTTGAGCGTGGTTTTGTAgg                                                                  >  2:102952/1‑143 (MQ=11)
                                                                                aTAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                <  2:10886/143‑1 (MQ=37)
                                                                                    ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                            >  1:271863/1‑143 (MQ=38)
                                                                                          ttAGCGTGGTTTTGTGGGTCGGATACGGCGTTTACGCCGCATCAACAATTATGAACTTATTGGTCGCTTGCCGCGTCTTATCAGGCCTACGCCTGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCg                                                      <  2:1409667/143‑1 (MQ=11)
                                                                                              cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                      <  1:377310/59‑1 (MQ=255)
                                                                                              cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCg                                                                                                                                      >  2:377310/1‑59 (MQ=38)
                                                                                              cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa                                                  >  1:901242/1‑143 (MQ=39)
                                                                                                       gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa                                                                                                                                          <  1:900633/46‑1 (MQ=255)
                                                                                                        tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgc                                                                                                                           <  2:1114941/60‑1 (MQ=255)
                                                                                                        tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgc                                                                                                                           >  1:1114941/1‑60 (MQ=255)
                                                                                                         ggttgggATAGGGGGTTTTCGACGCGCACGTCATCAACGCCTGATGCGACGCTTGCTGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg                                       <  2:1080157/138‑1 (MQ=11)
                                                                                                           gTCGGATGCGGCGTAAACGCCTTATCCGACATCAACGCCTGATGCGACACTTGCCGCGTCTTATCAGGCCTACGACATACAGCGCAAAATCCAGATATAGCGTGAGTTCATCCGTCGGCTAAGCCGCTTATGCCGCATCCGAc                                     >  2:1244665/1‑143 (MQ=2)
                                                                                                           gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGCCAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGGGTAAGGCGTTTCTGCCGCATCCCAc                                     >  2:843585/1‑143 (MQ=14)
                                                                                                                    ggCGTTTACGCCGCATCCGACATCAACGCCTAATGCCACGCGTGGCTCGTCTTATCAGGCCTACGCCAGACAGCGCAACAGCCTGATTTAGCGTGATTTTGCAGCGCGGCTAAAACGTTTATGCCGCATCCGTCGTCAACGcc                            >  2:311739/1‑143 (MQ=11)
                                                                                                                     gggTTTACGCCGCGTCCGATGTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt                           <  2:484899/141‑1 (MQ=255)
                                                                                                                     gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt                           >  1:489909/1‑143 (MQ=38)
                                                                                                                       gTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                   <  1:92198/53‑1 (MQ=255)
                                                                                                                       gTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                   >  2:92198/1‑53 (MQ=255)
                                                                                                                         ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc                                                                                                                        <  2:1040382/46‑1 (MQ=255)
                                                                                                                         ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTc                                                                                                                        >  1:1040382/1‑46 (MQ=255)
                                                                                                                             gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTa                                                                                 <  2:916037/81‑1 (MQ=38)
                                                                                                                             gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTATGCCGCCTCCGACATCAACGCCTGATGCGAc                   >  2:745501/1‑143 (MQ=21)
                                                                                                                             gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACACCGCACGAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGAAATCAACGCCTGATGCGAc                   >  2:468674/1‑143 (MQ=255)
                                                                                                                               cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGc                 >  1:514291/1‑143 (MQ=35)
                                                                                                                                  aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGTCGTATCCGCCATCGACGCGTGATGCGACGcgtc              >  1:263020/1‑140 (MQ=11)
                                                                                                                                  aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGGttc              >  1:991521/1‑142 (MQ=25)
                                                                                                                                    ccGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTGCGGAGGTCGGATCAGGCGTTTTTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc            >  1:903897/1‑143 (MQ=11)
                                                                                                                                      gACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc          >  2:1424767/1‑143 (MQ=35)
                                                                                                                                        cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt        <  2:704960/143‑1 (MQ=34)
                                                                                                                                         gtgaaCGCCGGTTGCGGCGCTTGCCGCCTCTTAGCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGCCGGATAGGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc       <  2:137544/140‑1 (MQ=1)
                                                                                                                                           gaaCGCCTGCGGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCtt     <  2:885605/142‑1 (MQ=14)
                                                                                                                                            aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCGTa    >  1:549768/1‑143 (MQ=17)
                                                                                                                                             aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg                                                                                                          <  1:522103/40‑1 (MQ=37)
                                                                                                                                              cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc  <  1:1189738/143‑1 (MQ=11)
                                                                                                                                              |                                                                                                                                              
TAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATC  >  NC_000913/4295918‑4296202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: