Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,293,719 | A→G | 17.8% | E107G (GAG→GGG) | lpoA → | OM lipoprotein stimulator of MrcA transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,293,719 | 0 | A | G | 17.8% | 149.1 / 3.3 | 70 | E107G (GAG→GGG) | lpoA | OM lipoprotein stimulator of MrcA transpeptidase |
Reads supporting (aligned to +/- strand): ref base A (16/39); new base G (12/2); total (29/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.72e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGA > NC_000913/3293492‑3293952 | tCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCCACTACCGCAAGAACTGGACAAGGCTCAGCGTCCCCGGAAAACACGACCGGCGGGAGGGAGTTAAATgg > 1:654635/1‑239 (MQ=255) ccccGATCAGTCCCCCTCTTAGCTTCGGGGCACGTCGCAGGCGTATTCTGTCTTTTAGCTTGAGCAGATGCCGCAAAGCCTTGATGATGCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTGTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTgg < 2:586726/238‑1 (MQ=255) ccccATCATTCCACTGCTTATAGGCCGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACGCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGgc < 2:567823/239‑1 (MQ=255) tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTGGTTGTTTAACCAACTACCGCAAGAACTGAACAATGCTCCGCGTCGCGGGGAAAAACTGCTGGCGGGAGGAATTAAACTGACG‑CAGaaaag > 1:148822/1‑237 (MQ=255) tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTGGTTGGTTTACCAACACCCCGAAAAACTGAACGATGCGCAGCGGCGCGGGAAAACACCGCCGGCGGGAGGGAATAAAATGGGG‑CAaaaaaa > 2:1089015/1‑237 (MQ=255) tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTCCTGGCGGTAGGGATTAAACTGGGG‑CAGaaaaa > 1:331443/1‑237 (MQ=255) atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTAGGTTTTTAACCAAATACACCAAAAACTGAAGCATGCTCAGCGGTGCGGGGAAAAACTCACGGGGGGAAGGATAAAACCGGGC‑CAGAAAAATTTTTTTGgc > 2:737238/1‑239 (MQ=255) atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTGCTGGGGGGAGGGATTAAACTTGCG‑CCGAAAGAATTTTCTggg > 1:25443/1‑238 (MQ=255) atatGCAGGGCACGGCACAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTTGAGATTAAACTGGGG‑CCGGAAGAttttttcggc > 1:690815/1‑233 (MQ=255) tggcggggccGGCGCCGGTTGATTTTGCCTTTTTTTTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGAACAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGgcgcg < 2:916841/230‑1 (MQ=255) cAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACTGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTGCTGGCGGTAGGGGTTTAAATTGGG‑CAGAAAGAtttttttgggcgcgc > 1:568091/1‑228 (MQ=255) cAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTGCTGGCGGTAAGGGTTAAACTGGCG‑CGGAAAGATTTTGCTGTCGGGCa > 1:370870/1‑239 (MQ=255) gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGTGAAAACACTGCTGGCGGCTGAGGTTAAAATGGTC‑CAGAAAAATTTTGCTGGCGCGCaaa > 1:443639/1‑239 (MQ=255) gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCCCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CCGAAAAATTTTTCTGgcggcccaa > 1:279224/1‑233 (MQ=255) cACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGGGATTAAACAAGCG‑GAAAAAAAGTTTTCTGGCGCGCAAAACt > 1:766080/1‑239 (MQ=255) cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTgg < 1:460034/204‑1 (MQ=255) cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTg < 1:16652/222‑1 (MQ=255) ggCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCCCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg < 1:282901/238‑1 (MQ=255) gCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGGGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAACGTCGCGAGAAAACACTTCTGGCGGGAAGGATTAAAATGGCGCCAGAAA‑ATTTTTCTGGGCGGCCAAACTTGTTggggga > 1:508132/1‑235 (MQ=255) cTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg < 1:198084/236‑1 (MQ=255) ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAACGTCGCCAGAAAACACTGCTGGCGGTAGAGATTAAACTGGGG‑ACGAAAAATTTTTCTGGCGGCCAAAACTTTCTGGCGAAAATca > 1:32230/1‑239 (MQ=255) cTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATcaca < 1:100986/239‑1 (MQ=255) ttttttCTTCAGCAGATGCCGCAAAGCTTTGATGATACCAGGATCAACTGGCAATTCCGCGCCGTTCGTGAACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc < 2:279224/239‑1 (MQ=255) ttttATCTTCAGCCGATGCCGCAAAGCTCTGAGGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTGGAGTTGGTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACCCCTGc < 2:455065/239‑1 (MQ=255) ttttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc < 1:572872/239‑1 (MQ=255) ttttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACGGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc < 1:737238/239‑1 (MQ=255) ttttATCGTCAGCAGATGCAGCAAAGCTCTGATGAAACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc < 1:842190/239‑1 (MQ=255) tCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGCG‑CCGAAAAATTTTTCTGGGCGGCAAAACTTGTTGGGGGAAAACACAccctcccgtttta > 1:224223/1‑228 (MQ=255) agATGCAGCAAAGCTTTGATGATACCAGTATCAACTGGCAATTGCTCGCAATGCGTGCACTGGTGAAAGAAGGGAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGGG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGGGAAAATCACACCTGCCGATTTAGa‑a < 1:953423/236‑1 (MQ=255) attccAGGATCAACGGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGATTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTgg < 1:53835/236‑1 (MQ=255) aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTTGGG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCCCACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTAATGGCC‑AGGaaaaa > 1:655619/1‑237 (MQ=255) aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAACCAACTACCGCAAGAAATGAACGATGCTCACCGTCGCGAGAAAACACTGCTGCCGGTAGGGATTAAACTGGCG‑CCGAAAGATTTTTCTGGCGCGCAAAACTTGCGGGCGCAAAACACACCCCCCGCTTTTGACACAAAACACAACAA‑C‑CGTTACCGGCA‑GGaaaaaaaa > 2:882185/1‑237 (MQ=255) aaTTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAgc < 1:733545/211‑1 (MQ=255) ttcTCGCCTTTCGTGCACTGGGGAAAGAGGTAAAAACCGGGCAGGCGTTTGAGTTGTTAAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATgccagc < 2:370870/237‑1 (MQ=255) cGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGTGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTcc < 1:147627/239‑1 (MQ=255) tccttGGTAAAACAAGGGAAACCCGGCCAGGCGTTTGGGTTTTTAAACCAATTACCGGAAGAATTGAACGATGCCCGGCGTCGCGATAAACCCCTGCTGGCGGTAGAGATTAAACTGGGG‑CAGAAGGATTTTGCGGGCGCGCAAAATGTGGTGGCGAAAATCACCCCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTc < 2:803000/235‑1 (MQ=255) gCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGGAAGAACGGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTc < 1:494202/239‑1 (MQ=255) acgggTAAAAGATGGTAAACCCGGGAAGGGGTTGGTGTTTTTTACCCATCTACCGCAAGACCTGAACGATGCTCAGCTTGGCGAGAAAACCCGGCTGGCGTTAGAGATTAAACTGGCG‑CAGAAAGATTTGGCTGGCGCGCAAAACTTGCTGGCGAAAATCCCACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa < 2:655619/236‑1 (MQ=255) aCTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa < 1:271078/239‑1 (MQ=255) gtaaaaGAGGGTAAAACCGGGCAGGCGTTTGAGTGTTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCCCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTcc < 1:392904/227‑1 (MQ=255) gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCCAAACTTGCTGGCGCAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGGAAAAATACATGCCAGCCAAGGGCGcccttcccttag > 1:964922/1‑237 (MQ=255) gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGAATTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGGTTTTGA‑ACAAAAC‑CCGCAAGCGCGTTACTTGCA‑GGGCAAAAACGGAGCCAGCCAGGGGGGGCCCTCCCTTGa > 1:368601/1‑239 (MQ=255) ggTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAATTACCGCAAGAACTGAACGATGCTCAGCTACGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTGGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATg < 1:262367/205‑1 (MQ=255) gTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa < 1:168328/225‑1 (MQ=255) gTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTTGCGGTAGGGGTTAAAATTGCC‑CAGAAAGATTTTTCCGGCGCGCAAAACTTGCTGGGGAAAATAAACACTGACCATTTTAA‑ACAAAAC‑CAGCAAACGCGTTAATTGCAGGGCAAAAAAC‑ATACCAACCAGCGGCGGCCCTCCACTTATTTaaaggag > 2:687772/1‑233 (MQ=255) tAAACCGGGGCCGGCGTTTGGTTTGTTAACCCAACTCCCGAAAGACCTGAACGATGGTCGGGGGCGGGAGAAAACCCTGCGGGCGGTAGAGATTAACCTGGCG‑CAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg < 2:690815/237‑1 (MQ=255) cGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCCCGGCGGCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTaa < 1:708272/239‑1 (MQ=255) gggCAGGTGGTTGAGTTGTTTAACCAACTACCGCAAGGACTGAACGATGCTCAGCGTCGCGAGGAAACACTGCTGGCGGTAGCGCTTAAACTCGCG‑CAGAAAGAATTTTCTGGAGCGACCAACTTGCTGGGGAAAAACACAACGCACGATTTTTA‑ACAAAAA‑CAACAAGCGCGTTACTGGAC‑GGCAAAAATACATGCCCCCCACGGGCGCGCTTCCACTTAGTTACAGcccgcgaaaat > 2:815333/1‑234 (MQ=255) gggCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAt < 1:614423/239‑1 (MQ=255) ggTTGGGTTTTTTAACCAATTTCCGCAAGAACTGAACGAGGCTCGGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAgg < 2:368601/239‑1 (MQ=255) cAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCACGCAAAACTTGCTGGAGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑gcgc < 1:312020/239‑1 (MQ=255) tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGc < 1:443058/138‑1 (MQ=255) tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGGTTTACTTCGCCCGTTAAATGCTCAGGAACCGCTGCTTT‑GCGCcaaa > 1:412680/1‑239 (MQ=255) tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTACTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGc > 2:443058/1‑138 (MQ=255) aaagaCTTAACGTTGCACAGCGTCGCGGGAAACCCTGGCTGGCGGTAGAAATTAACCTGGCC‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGGGAAAATCACACCTTCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGaaaaa < 1:1085733/235‑1 (MQ=255) aaaaaCGGAACGATGCCAAGGGTCGCGAGAAAACATTGCTGGCGGTAGGGATTAAACTGGCC‑CAGAAAGATTTTCCTGGCGCGAAAAACTTGCTGGCGAAAATCACCCCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCATGAACCGCTGCTTG‑GCGCGAAAGaaaaa < 1:187281/236‑1 (MQ=255) aaCTGAACGATGCTCAGCGTCGCGAAAAAACCCTGGTGGCGGTAGAAATTAAACTGGCG‑CAGAAATATTTTGCTGGAGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAAcag < 1:270666/239‑1 (MQ=255) aCTGAACGATGCTCATCTTCGCGATAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTGGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAAcag < 1:211152/238‑1 (MQ=255) tGAACGATACTCAGGGTCGCGAAAAAACCCTGCTGGCGGTAGAAATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACCCCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAAcagcag < 1:429779/239‑1 (MQ=255) aaCGATACTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGACGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGGCAGCACGGGGCGTCCTGCCATTGATTTACTGCGGCCGTTAATTGCTCAGGAACCGCCGCTTG‑G‑GCGAaaaaaaaaaaacaaaaa > 2:429779/1‑230 (MQ=255) cccAGGGTCGGGAGAAAACCCTGCTGGCGGTAGAGATTAACCTGGCG‑CAGAAAGATTTTGCTGGCGGGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGa < 1:882185/236‑1 (MQ=255) tCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGGCCTTCCATTGATTTACTGCGCGCGTTAATTGCCCAGGAACCGCTGCTTG‑GCGGGAAAGAAAAACAGCCGAATATTGAt > 1:547341/1‑238 (MQ=255) cgcgAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAAGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGGAAGAAAAACAGCAGAATATTGATGCCACCTg > 1:177022/1‑239 (MQ=255) cgcgAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCCCGTTACTGGCA‑GGCAAAAAACGATTCCAGCCAGGGGCGGCCTTCCCTTTATTTACTCGGGCCGGTAATTTCCCCAGAACCCCTCCTTG‑GCGCGCAAAAAAAACAGCAAAAAATTTATGCCAcccg > 2:1019687/1‑237 (MQ=255) cgAGAAAACACTGCTGGCGGTAGAGATTAAAATGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTTCCGATTTAGA‑ACACAAC‑CAGCAAGCGCGTTACCTGCC‑GGCAAAAATCAACACCAGCCAGGGGCGGACTTCCCTCGATTTACTGCGCGCGTTAATTGCCCAGCACCCGCTGCCTGTCCGCCAAGAAAAAACA‑CAGAAAATTTCTACCACCCGGc > 2:874352/1‑239 (MQ=255) ccctgctgGCGGTAGAGATAAAACTGGCG‑CAGAAAGATTTTGCTGGCGCACAAAACTTGCTGGCGAAAATCACCCCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc < 1:118567/237‑1 (MQ=255) ccctgcggGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAACCTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCAGTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc < 1:455777/237‑1 (MQ=255) cccggctgGCGGTAGGAATTAAACTGGCG‑AAGAAAGATTTTGCTGGCGCCAAAAACTTGCTGGCGAAAACCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc < 1:330204/235‑1 (MQ=255) cacTGATGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCCCACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc < 1:768937/239‑1 (MQ=255) ggTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCCGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGGGCCTtcctccctct > 1:336958/1‑235 (MQ=255) | TCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCG‑CAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGA‑ACAAAAC‑CAGCAAGCGCGTTACTGGCA‑GGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTG‑GCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGA > NC_000913/3293492‑3293952 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |