Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,293,719 A→G 17.8% E107G (GAG→GGG)  lpoA → OM lipoprotein stimulator of MrcA transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,293,7190AG17.8% 149.1 / 3.3 70E107G (GAG→GGG) lpoAOM lipoprotein stimulator of MrcA transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (16/39);  new base G (12/2);  total (29/41)
Fisher's exact test for biased strand distribution p-value = 1.72e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGA  >  NC_000913/3293492‑3293952
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
tCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCCACTACCGCAAGAACTGGACAAGGCTCAGCGTCCCCGGAAAACACGACCGGCGGGAGGGAGTTAAATgg                                                                                                                                                                                                                                     >  1:654635/1‑239 (MQ=255)
ccccGATCAGTCCCCCTCTTAGCTTCGGGGCACGTCGCAGGCGTATTCTGTCTTTTAGCTTGAGCAGATGCCGCAAAGCCTTGATGATGCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTGTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTgg                                                                                                                                                                                                                                     <  2:586726/238‑1 (MQ=255)
 ccccATCATTCCACTGCTTATAGGCCGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACGCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGgc                                                                                                                                                                                                                                    <  2:567823/239‑1 (MQ=255)
          tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTGGTTGTTTAACCAACTACCGCAAGAACTGAACAATGCTCCGCGTCGCGGGGAAAAACTGCTGGCGGGAGGAATTAAACTGACGCAGaaaag                                                                                                                                                                                                                          >  1:148822/1‑237 (MQ=255)
          tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTGGTTGGTTTACCAACACCCCGAAAAACTGAACGATGCGCAGCGGCGCGGGAAAACACCGCCGGCGGGAGGGAATAAAATGGGGCAaaaaaa                                                                                                                                                                                                                          >  2:1089015/1‑237 (MQ=255)
          tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTCCTGGCGGTAGGGATTAAACTGGGGCAGaaaaa                                                                                                                                                                                                                          >  1:331443/1‑237 (MQ=255)
                    atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTAGGTTTTTAACCAAATACACCAAAAACTGAAGCATGCTCAGCGGTGCGGGGAAAAACTCACGGGGGGAAGGATAAAACCGGGCCAGAAAAATTTTTTTGgc                                                                                                                                                                                                                >  2:737238/1‑239 (MQ=255)
                    atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTGCTGGGGGGAGGGATTAAACTTGCGCCGAAAGAATTTTCTggg                                                                                                                                                                                                                >  1:25443/1‑238 (MQ=255)
                    atatGCAGGGCACGGCACAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTTGAGATTAAACTGGGGCCGGAAGAttttttcggc                                                                                                                                                                                                                >  1:690815/1‑233 (MQ=255)
                       tggcggggccGGCGCCGGTTGATTTTGCCTTTTTTTTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGAACAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGgcgcg                                                                                                                                                                                                             <  2:916841/230‑1 (MQ=255)
                         cAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACTGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTGCTGGCGGTAGGGGTTTAAATTGGGCAGAAAGAtttttttgggcgcgc                                                                                                                                                                                                           >  1:568091/1‑228 (MQ=255)
                         cAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGGGAAAACACTGCTGGCGGTAAGGGTTAAACTGGCGCGGAAAGATTTTGCTGTCGGGCa                                                                                                                                                                                                           >  1:370870/1‑239 (MQ=255)
                           gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGTGAAAACACTGCTGGCGGCTGAGGTTAAAATGGTCCAGAAAAATTTTGCTGGCGCGCaaa                                                                                                                                                                                                         >  1:443639/1‑239 (MQ=255)
                           gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCCCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCCGAAAAATTTTTCTGgcggcccaa                                                                                                                                                                                                         >  1:279224/1‑233 (MQ=255)
                              cACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGGGATTAAACAAGCGGAAAAAAAGTTTTCTGGCGCGCAAAACt                                                                                                                                                                                                      >  1:766080/1‑239 (MQ=255)
                                   cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTgg                                                                                                                                                                                                                                     <  1:460034/204‑1 (MQ=255)
                                   cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTg                                                                                                                                                                                                                  <  1:16652/222‑1 (MQ=255)
                                       ggCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCCCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg                                                                                                                                                                                              <  1:282901/238‑1 (MQ=255)
                                        gCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGGGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAACGTCGCGAGAAAACACTTCTGGCGGGAAGGATTAAAATGGCGCCAGAAAATTTTTCTGGGCGGCCAAACTTGTTggggga                                                                                                                                                                                            >  1:508132/1‑235 (MQ=255)
                                         cTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCg                                                                                                                                                                                              <  1:198084/236‑1 (MQ=255)
                                             ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAACGTCGCCAGAAAACACTGCTGGCGGTAGAGATTAAACTGGGGACGAAAAATTTTTCTGGCGGCCAAAACTTTCTGGCGAAAATca                                                                                                                                                                                       >  1:32230/1‑239 (MQ=255)
                                               cTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATcaca                                                                                                                                                                                     <  1:100986/239‑1 (MQ=255)
                                                    ttttttCTTCAGCAGATGCCGCAAAGCTTTGATGATACCAGGATCAACTGGCAATTCCGCGCCGTTCGTGAACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                                                                <  2:279224/239‑1 (MQ=255)
                                                    ttttATCTTCAGCCGATGCCGCAAAGCTCTGAGGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTGGAGTTGGTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACCCCTGc                                                                                                                                                                                <  2:455065/239‑1 (MQ=255)
                                                    ttttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                                                                <  1:572872/239‑1 (MQ=255)
                                                    ttttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACGGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                                                                <  1:737238/239‑1 (MQ=255)
                                                    ttttATCGTCAGCAGATGCAGCAAAGCTCTGATGAAACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGc                                                                                                                                                                                <  1:842190/239‑1 (MQ=255)
                                                            tCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGCGCCGAAAAATTTTTCTGGGCGGCAAAACTTGTTGGGGGAAAACACAccctcccgtttta                                                                                                                                                                        >  1:224223/1‑228 (MQ=255)
                                                                 agATGCAGCAAAGCTTTGATGATACCAGTATCAACTGGCAATTGCTCGCAATGCGTGCACTGGTGAAAGAAGGGAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGGAGAGATTAAACTGGGGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGGGAAAATCACACCTGCCGATTTAGaa                                                                                                                                                                     <  1:953423/236‑1 (MQ=255)
                                                                                      attccAGGATCAACGGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGATTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTgg                                                                                                                                            <  1:53835/236‑1 (MQ=255)
                                                                                                  aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGGTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTTGGGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCCCACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTAATGGCCAGGaaaaa                                                                                                                                 >  1:655619/1‑237 (MQ=255)
                                                                                                  aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAACCAACTACCGCAAGAAATGAACGATGCTCACCGTCGCGAGAAAACACTGCTGCCGGTAGGGATTAAACTGGCGCCGAAAGATTTTTCTGGCGCGCAAAACTTGCGGGCGCAAAACACACCCCCCGCTTTTGACACAAAACACAACAACCGTTACCGGCAGGaaaaaaaa                                                                                                                               >  2:882185/1‑237 (MQ=255)
                                                                                                        aaTTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAgc                                                                                                                                                      <  1:733545/211‑1 (MQ=255)
                                                                                                           ttcTCGCCTTTCGTGCACTGGGGAAAGAGGTAAAAACCGGGCAGGCGTTTGAGTTGTTAAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATgccagc                                                                                                                      <  2:370870/237‑1 (MQ=255)
                                                                                                                      cGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGTGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTcc                                                                                                           <  1:147627/239‑1 (MQ=255)
                                                                                                                         tccttGGTAAAACAAGGGAAACCCGGCCAGGCGTTTGGGTTTTTAAACCAATTACCGGAAGAATTGAACGATGCCCGGCGTCGCGATAAACCCCTGCTGGCGGTAGAGATTAAACTGGGGCAGAAGGATTTTGCGGGCGCGCAAAATGTGGTGGCGAAAATCACCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTc                                                                                                        <  2:803000/235‑1 (MQ=255)
                                                                                                                         gCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGGAAGAACGGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTc                                                                                                        <  1:494202/239‑1 (MQ=255)
                                                                                                                           acgggTAAAAGATGGTAAACCCGGGAAGGGGTTGGTGTTTTTTACCCATCTACCGCAAGACCTGAACGATGCTCAGCTTGGCGAGAAAACCCGGCTGGCGTTAGAGATTAAACTGGCGCAGAAAGATTTGGCTGGCGCGCAAAACTTGCTGGCGAAAATCCCACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                                                      <  2:655619/236‑1 (MQ=255)
                                                                                                                           aCTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                                                      <  1:271078/239‑1 (MQ=255)
                                                                                                                               gtaaaaGAGGGTAAAACCGGGCAGGCGTTTGAGTGTTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCCCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTcc                                                                                                           <  1:392904/227‑1 (MQ=255)
                                                                                                                               gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCCAAACTTGCTGGCGCAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGGAAAAATACATGCCAGCCAAGGGCGcccttcccttag                                                                                                  >  1:964922/1‑237 (MQ=255)
                                                                                                                               gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGAATTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGGTTTTGAACAAAACCCGCAAGCGCGTTACTTGCAGGGCAAAAACGGAGCCAGCCAGGGGGGGCCCTCCCTTGa                                                                                                  >  1:368601/1‑239 (MQ=255)
                                                                                                                                        ggTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAATTACCGCAAGAACTGAACGATGCTCAGCTACGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTGGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATg                                                                                                                           <  1:262367/205‑1 (MQ=255)
                                                                                                                                         gTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCa                                                                                                      <  1:168328/225‑1 (MQ=255)
                                                                                                                                         gTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTTGCGGTAGGGGTTAAAATTGCCCAGAAAGATTTTTCCGGCGCGCAAAACTTGCTGGGGAAAATAAACACTGACCATTTTAAACAAAACCAGCAAACGCGTTAATTGCAGGGCAAAAAACATACCAACCAGCGGCGGCCCTCCACTTATTTaaaggag                                                                                        >  2:687772/1‑233 (MQ=255)
                                                                                                                                          tAAACCGGGGCCGGCGTTTGGTTTGTTAACCCAACTCCCGAAAGACCTGAACGATGGTCGGGGGCGGGAGAAAACCCTGCGGGCGGTAGAGATTAACCTGGCGCAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg                                                                                         <  2:690815/237‑1 (MQ=255)
                                                                                                                                                cGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCCCGGCGGCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTaa                                                                                 <  1:708272/239‑1 (MQ=255)
                                                                                                                                                 gggCAGGTGGTTGAGTTGTTTAACCAACTACCGCAAGGACTGAACGATGCTCAGCGTCGCGAGGAAACACTGCTGGCGGTAGCGCTTAAACTCGCGCAGAAAGAATTTTCTGGAGCGACCAACTTGCTGGGGAAAAACACAACGCACGATTTTTAACAAAAACAACAAGCGCGTTACTGGACGGCAAAAATACATGCCCCCCACGGGCGCGCTTCCACTTAGTTACAGcccgcgaaaat                                                                                >  2:815333/1‑234 (MQ=255)
                                                                                                                                                 gggCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAt                                                                                <  1:614423/239‑1 (MQ=255)
                                                                                                                                                         ggTTGGGTTTTTTAACCAATTTCCGCAAGAACTGAACGAGGCTCGGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAgg                                                                        <  2:368601/239‑1 (MQ=255)
                                                                                                                                                                         cAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCACGCAAAACTTGCTGGAGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGgcgc                                                       <  1:312020/239‑1 (MQ=255)
                                                                                                                                                                             tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGc                                                                                                                                                          <  1:443058/138‑1 (MQ=255)
                                                                                                                                                                             tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGGTTTACTTCGCCCGTTAAATGCTCAGGAACCGCTGCTTTGCGCcaaa                                                   >  1:412680/1‑239 (MQ=255)
                                                                                                                                                                             tACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTACTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGc                                                                                                                                                          >  2:443058/1‑138 (MQ=255)
                                                                                                                                                                                   aaagaCTTAACGTTGCACAGCGTCGCGGGAAACCCTGGCTGGCGGTAGAAATTAACCTGGCCCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGGGAAAATCACACCTTCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa                                             <  1:1085733/235‑1 (MQ=255)
                                                                                                                                                                                   aaaaaCGGAACGATGCCAAGGGTCGCGAGAAAACATTGCTGGCGGTAGGGATTAAACTGGCCCAGAAAGATTTTCCTGGCGCGAAAAACTTGCTGGCGAAAATCACCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCATGAACCGCTGCTTGGCGCGAAAGaaaaa                                             <  1:187281/236‑1 (MQ=255)
                                                                                                                                                                                      aaCTGAACGATGCTCAGCGTCGCGAAAAAACCCTGGTGGCGGTAGAAATTAAACTGGCGCAGAAATATTTTGCTGGAGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag                                          <  1:270666/239‑1 (MQ=255)
                                                                                                                                                                                       aCTGAACGATGCTCATCTTCGCGATAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTGGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag                                          <  1:211152/238‑1 (MQ=255)
                                                                                                                                                                                         tGAACGATACTCAGGGTCGCGAAAAAACCCTGCTGGCGGTAGAAATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag                                       <  1:429779/239‑1 (MQ=255)
                                                                                                                                                                                           aaCGATACTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGACGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGGCAGCACGGGGCGTCCTGCCATTGATTTACTGCGGCCGTTAATTGCTCAGGAACCGCCGCTTGGGCGAaaaaaaaaaaacaaaaa                                    >  2:429779/1‑230 (MQ=255)
                                                                                                                                                                                                  cccAGGGTCGGGAGAAAACCCTGCTGGCGGTAGAGATTAACCTGGCGCAGAAAGATTTTGCTGGCGGGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGa                               <  1:882185/236‑1 (MQ=255)
                                                                                                                                                                                                   tCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGGCCTTCCATTGATTTACTGCGCGCGTTAATTGCCCAGGAACCGCTGCTTGGCGGGAAAGAAAAACAGCCGAATATTGAt                              >  1:547341/1‑238 (MQ=255)
                                                                                                                                                                                                          cgcgAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAAGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGGAAGAAAAACAGCAGAATATTGATGCCACCTg                      >  1:177022/1‑239 (MQ=255)
                                                                                                                                                                                                          cgcgAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCCCGTTACTGGCAGGCAAAAAACGATTCCAGCCAGGGGCGGCCTTCCCTTTATTTACTCGGGCCGGTAATTTCCCCAGAACCCCTCCTTGGCGCGCAAAAAAAACAGCAAAAAATTTATGCCAcccg                      >  2:1019687/1‑237 (MQ=255)
                                                                                                                                                                                                            cgAGAAAACACTGCTGGCGGTAGAGATTAAAATGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTTCCGATTTAGAACACAACCAGCAAGCGCGTTACCTGCCGGCAAAAATCAACACCAGCCAGGGGCGGACTTCCCTCGATTTACTGCGCGCGTTAATTGCCCAGCACCCGCTGCCTGTCCGCCAAGAAAAAACACAGAAAATTTCTACCACCCGGc                    >  2:874352/1‑239 (MQ=255)
                                                                                                                                                                                                                    ccctgctgGCGGTAGAGATAAAACTGGCGCAGAAAGATTTTGCTGGCGCACAAAACTTGCTGGCGAAAATCACCCCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc            <  1:118567/237‑1 (MQ=255)
                                                                                                                                                                                                                    ccctgcggGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAACCTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCAGTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc            <  1:455777/237‑1 (MQ=255)
                                                                                                                                                                                                                    cccggctgGCGGTAGGAATTAAACTGGCGAAGAAAGATTTTGCTGGCGCCAAAAACTTGCTGGCGAAAACCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc            <  1:330204/235‑1 (MQ=255)
                                                                                                                                                                                                                    cacTGATGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGAAAAACTTGCTGGCGAAAATCCCACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc            <  1:768937/239‑1 (MQ=255)
                                                                                                                                                                                                                              ggTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCCGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGGGCCTtcctccctct  >  1:336958/1‑235 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              
TCCCGATCAGTCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGCAAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTGCTGGCGGTAGAGATTAAACTGGCGCAGAAAGATTTTGCTGGCGCGCAAAACTTGCTGGCGAAAATCACACCTGCCGATTTAGAACAAAACCAGCAAGCGCGTTACTGGCAGGCAAAAATCGATGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGA  >  NC_000913/3293492‑3293952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: