| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,983,147 | C→A | 9.5% | Q64K (CAA→AAA) | aslB → | putative AslA‑specific sulfatase‑maturating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,983,147 | 0 | C | A | 9.5% | 215.0 / 2.5 | 82 | Q64K (CAA→AAA) | aslB | putative AslA‑specific sulfatase‑maturating enzyme |
| Reads supporting (aligned to +/- strand): ref base C (56/17); new base A (2/7); total (58/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGC > NC_000913/3982911‑3983380 | aTTTCCCCCTTAACGTTTTTAAGGATGGCTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGGGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCaa < 2:672340/239‑1 (MQ=255) tCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCCAGCAAATGGACGATGACACGCTGGAACCGCATGGCCGTCACAATATCGCAGCAAGCGAACGCCaaaaa > 2:101926/1‑238 (MQ=255) cccTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTCTCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACCCTGGAAGCGTATGTCCGTCCCTATATCGCGGCCAGCGAACCGCAAAACGaa > 1:561632/1‑239 (MQ=255) cccTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCACCGAACGGCAAAACGaa > 1:834915/1‑239 (MQ=255) cccTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTACCCATATCGCGGCCAGCGAACCGCAAAACGaa > 1:217510/1‑239 (MQ=255) cccTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACCCTGGAAGCGCATGTCCGTCACTATATCGCTGCCGGCGAACCGCAAAACGaa > 1:342336/1‑239 (MQ=255) aaCGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTg > 1:403001/1‑237 (MQ=255) ggacaggcTTCGGGCAAGGGGCGACCATTCTTCAACCGGTTCCAAGGCGGGCTTTTAATTTGAGGGGGAACCCGAGCGGTTCCGATTGTAATCTGAACTGTGACTCCTTGTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTgg < 2:14820/232‑1 (MQ=255) tGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGGGCGGTTCCGATTGTAATCTGAACTGTGACTACGGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTAACGCATATGGACGATGACACGCTGGAAGCGTAGTACCGTCACTATCTCGCTGCCGGCCAACCGAAAAAAGAAGTGGCTGTTAcacggcgcg > 2:426662/1‑231 (MQ=255) tGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGCTGACACGCTTGAACCGAATGTCCGTCACTATATCGCTGCCAGCGACCCGCAAAACGAAGTGGCTTTTAACTTGCAgg > 1:517985/1‑239 (MQ=255) tGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTCACGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACCCTGGAAACGTATGTCCGTCCCTATATCGCGGCCAGCGAACCGCCAAACGCAGAGGCTTTAACCTggacgg > 2:989114/1‑235 (MQ=255) aCTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGCCCGCAACTATATCGATGCCAGCGAACCGCAAAACGCAGAGGCTTTTACCTGCCAgggg > 2:942093/1‑238 (MQ=255) cAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCACCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGgcggcg > 1:965734/1‑239 (MQ=255) ggAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCaa > 1:1081255/1‑238 (MQ=255) gAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGactgcc > 1:14820/1‑232 (MQ=255) gAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAAc > 1:281101/1‑238 (MQ=255) ggCGGCCATGCTTCAACAGGTTTCATAGCGTTCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGGGACTACTGTTTTTATCTGGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCaa < 2:217510/235‑1 (MQ=255) aGCGACCATGCTTCAACAGGGTCCAACGCCGTCTTTTCATGTGGTGGCGAAACCGAGCGGTTCCGATTGTAATCCGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACg < 2:281101/238‑1 (MQ=255) aCCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGGTGGCGAAACCGCGCGGTTCCGATTGTAATCTGAACTGTGGCTACTGTTTTTATCTCCAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGTACGCTGACACGACGGAACCGTATGCCCGTCACTATATTCCTGCCACCGAAACGCAAAACGAAGAGGCTTTTACCTGGCAGAGCGGCGCACAAAcacaac > 2:280136/1‑233 (MQ=255) aCCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCAAACCAACGCAAcc > 2:1014679/1‑238 (MQ=255) ctgcAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGCAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGCAAGGGCGGCGAACCAACGcaccccgg > 1:135972/1‑229 (MQ=255) ctgcAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCgg > 1:695350/1‑236 (MQ=255) aCAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTCTATCCCTGCCAGCGAACCGCAAAACGAATGGGCTTTTACCTGGCAGGGGGGCGAAACAAACCAACACGGGCTggggg > 2:176840/1‑236 (MQ=255) gTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGc < 1:571715/191‑1 (MQ=255) tCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCCCTATATCGCTGCCACCGAACCGCAAAACGACGTGGCTTTTACCTGGCATGGCGGCGAACCAACCCCACCCGGGCTGGCGTTTTAcc > 2:93504/1‑239 (MQ=255) tCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCCCTGCCAGCGAACCGCCAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAAAGCTACCCGGGCTGGCGTTTTAc > 1:26018/1‑238 (MQ=255) aCGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGCCCGTCACTATATCGCTGCCACCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGGGGACCAACGCAACACGGGGTGGCGTTTTAccgccg > 2:553616/1‑239 (MQ=255) tGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGACGTGGCTTTTACCTGGCAGGGCGGCGATCCCACGCTACTCGGGCTGGCGTTTTCCCgccgtgccg > 1:608601/1‑239 (MQ=255) tGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCgccgtgccg > 1:39800/1‑239 (MQ=255) atggaagggCGAACCCGGGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAACCAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTa < 1:913948/232‑1 (MQ=255) aTGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGATAAGCCAGTCACGCATATGGACGATGACACGCTGGAACCGTATGTCCGTCACCATATCGCTGCCAGCGAACCGCAAACCGAAGGGGCTTTTCCCTGGCAGGGCGGCGAACCAACCCTACTCGGGCTTGCGTTTTACCCCAGGGCCGGAgg‑gcccccggc > 2:473217/1‑232 (MQ=255) aTGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTCTGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGACTTTTACCTTGCAGGGCGGCGAACCAACGCTACTCGTGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGc > 1:293654/1‑239 (MQ=255) aTGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGAAACACGAATGGGCTTTTACCTGGCAGGGCGGCGCACCAACGCAACTCGGGCTGGCGTTTTACCGCCGGGCCGGAGC‑GCAACAGGc > 2:83034/1‑239 (MQ=255) tgggcaaaCCGGGGGGTTCCAATTGTATCCTGAATTGTACCTTTGTTTTTTATCGCAAAAAACAACCCCTTACCCGCGAAAACCCAGTACCTAATAGGGCCGATGACCCGCTGGAGGCTGATGTCCGTCACTATACCGCTGCCAGCGAACCGAAAAACGAAGTGGCTTTTACCGGGCAGGGCGGCGAACCAACGTTCCCGGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCg < 2:164626/234‑1 (MQ=255) ggCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTCGCAGGCCGGCGAACCAACGCTACCCGGGCTGCGGTTTTACCGCCGTGCCGAAGG‑CCCACAGGGGa > 2:902036/1‑239 (MQ=255) aGCGGATCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTAACGCATATGGACGATGACACGCTGGAATCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGGACCAACGCCACTCGGGCTGGGGTTTTACCGCCGTGCCGGCGG‑TCAACAGGCGAAAAATGGGGc > 2:907911/1‑239 (MQ=255) gCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCATCGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTCCAGGCGAAATATggg > 1:158885/1‑235 (MQ=255) ctgATTTTAATCTGCAGTGTCACTACTTTTTTTATCACAAAACACAACCCCTTACCCGCGAAAACCCAGTCACGCATGGAGATGATGACCGGCTGGAAGCTTGTGTCCTGCACTATATCGCGGCAAGCAACCCGCAAACGGAAGTGGCTTTTCCTGGGCGGGGCGGCGAACCAACGCTACGCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAgg < 2:1052054/237‑1 (MQ=255) gaccaaaTCTGATCGGGTACTTCTTTTTTTATCTCAAAAACCAAGCCCTTCACCACGAAAAGCCAGTCACCAATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTCCCTGGCAGGGCGGCGAACCAACGCTCCTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGa < 2:1051445/232‑1 (MQ=255) ttGTAATCTGAACTGGGACTACTGTTTTTATCCCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAg < 1:90703/238‑1 (MQ=255) ttGAAATCTGAACTGTGACTCCTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACCCGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAg < 1:176840/238‑1 (MQ=255) aaTCTGAACTGTGACTACTGTTTTTATCTCGAAAACCAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGGATGTCCGTCACTATCTCGCTGCCAGCGAACCTCAAAACGAATTGGCTTTTCCCTGGACGGGCGGCGAACCAACACTACCCGGGATGGCGTGTTACCGCCGTGCCG‑AGCAGCTACAGACCAAACTTGGGGCTGGCAGTAAggaaaa > 2:361304/1‑234 (MQ=255) aaTCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGG‑CCTACAGGCGACAGATGGGGCTGGCAGGAAGGTAAg > 1:756656/1‑239 (MQ=255) aaTCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTCCAGGCGAAATATGGTGCTGGCAGGAAGATAAg > 1:134723/1‑239 (MQ=255) aaTCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAg > 1:11000/1‑239 (MQ=255) tgACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTAAAGGCGAAATATGGGGCTGGCAGGAAGATAAGTAACAgggc > 1:1051445/1‑234 (MQ=255) actactGTTTTTATCTCGAAAAACAATCCCTATACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGCAATATGGTGCTGGCAGGAAGATAAGTAACAACTTCCAg > 1:321045/1‑239 (MQ=255) tcctGTTTTAATCTCGAAAAACAATCCTTTTCCCGCGAAAAGCCAGTCACGCATAGGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGACCCGAAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGTTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAAc < 1:460422/226‑1 (MQ=255) actGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGAAAGATAAGGAACAGCTTCCaacct > 1:141029/1‑235 (MQ=255) actGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAAAATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACt > 1:54570/1‑239 (MQ=255) actGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCCCCGTGCCGTAGC‑GCTAAAGGGCAAATAGGGGGCTGGGGGGGAGAAAAAGAACAGGTTCCAgtaa > 2:1016045/1‑236 (MQ=255) ttttATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGGGCCGTAGC‑CCTCCAGGCGAAATTGGGGGCGGGCAGCAAGATAGATAACAGCTTcccaacaaaag > 2:936633/1‑229 (MQ=255) ttacccagAAAACCAATCCCTTTACCGCGAAAAGCCAGTACCGCATTGGGACGATGACACGCTGGAAGCGTATTTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCg < 1:1014679/232‑1 (MQ=255) ctcGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGAAAAAAGTTCCAAACAAAAGGCGggcg > 2:323812/1‑238 (MQ=255) tcGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGGGCTGGCAGGGAGAAAAGAAAAAGAGTCCAGACTAAAggggggggg > 2:1098846/1‑232 (MQ=255) aaaaCACTCCCTTTCCCGAAAAAACCCAGTCCCGCATATGGAGGATGACCCGCGGAAAGGTTTTGTCCGTCATTATATCGCTGCCAGCAAACCGCAAAACGAAGTGGCTTTTACCGGGCAGGGCGGCGAACCAACGCTACGCGGGGTGGCGTTTGACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTgg < 2:566285/239‑1 (MQ=255) ttACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATg > 1:566285/1‑237 (MQ=255) tACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGGGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATTTGGGTCGGGCAGGAAGATAAGTAAAAGCTACAAGCAAAACGGCGAGATGCCGGAGGAGGAAggggg > 2:493439/1‑237 (MQ=255) cgAAAAGCCAGTCACGCATATGGACGATGACACGCTGGACGCGTATGTACGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCtt > 2:725145/1‑92 (MQ=255) cgAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCtt < 1:725145/92‑1 (MQ=255) cgAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGt > 1:637424/1‑239 (MQ=255) gAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGTTGACGAATGGGGCGCGtt > 1:361870/1‑239 (MQ=255) gTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGa > 1:288179/1‑239 (MQ=255) tCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTCCTGCTGGATGACGAATGGTGCGCGTTTTTCCCggcc > 1:1069415/1‑237 (MQ=255) tGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTTACAGCTTCCCGACTAACGGCGGTCTGCTGGATGACGAAAGGGGCGCGTTTTTCCCgggagctcctttt > 2:469494/1‑228 (MQ=255) tGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATATGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGGGCGCGTTTCCCGCGGAGCATCATTTTCttgttg > 1:424073/1‑239 (MQ=255) tGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCttgttg > 1:305942/1‑239 (MQ=255) tGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATACTTTTCttgttg > 1:938586/1‑239 (MQ=255) tGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGGAACAGCTTCCCGACTAAAGGCGTGGTGCTGGATGACGAATGGTGCGCGGTTCCCGCGGGGCATCATTTTCtttttg > 2:1061349/1‑239 (MQ=255) tgtcctgctttattTCGCGCCCCGCGACCCTAAAACCGGAGTGGCTTTTACTGAGCAGGCGGGCGAACCACGGCTACCGGGGTGGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGcc < 2:468053/229‑1 (MQ=255) tGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGGGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGGGCATCATTTTCTTGTTGGTTTTACGCTGGATgtc > 1:830146/1‑236 (MQ=255) tGTCCGTCACCATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGGGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGCAGATACGGAACAGCTTCCAGACTAACGGCGGGCTGCTGACTGGCGAAGGGGGCGCGTTTCTCGCGGGGCATCATTTTTTGGTTGGGTTATcagtggggggg > 2:972932/1‑228 (MQ=255) tCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTCACGGCGTGCTGCTGGTTGACGAATGGGGGGCGTTTCTCGCGGGGCATACTTTTTTTGTTTGTTTTTCCATGGATGGCCCGcccg > 2:372959/1‑237 (MQ=255) tatCGCCGCCAGCAAACCGAAAAACGAAGTGGCTTTTCCCTGGCAGGGCGGCGAACCAACGCTACTGGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc < 1:323812/239‑1 (MQ=255) tagcggtcccACCGCACCGAAAACCGTGGTGGCTTTTCCCTGGCGGGGCGGCGACCCAACGTTACTCGGGCGGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc < 1:133531/231‑1 (MQ=255) taccGCTGCCGGCAAACGGAAAAACGAATTGGTTTTTCCCGGGCGGGGCGGCGAACCAACGCTACTCGGGCTGGCTTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCGCGCCTGAGATcc < 1:936633/236‑1 (MQ=255) aaccGCTCCCAGCGAACCGCAAACCGAGGGTGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATcc < 1:101926/236‑1 (MQ=255) gCTGCAAGCGAACCGCAAAGCGAAGGGGCTTGTCCCTGGCAGGGCGGCGAACCAATGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaa < 1:907911/239‑1 (MQ=255) gCTCCCGGCAAACCGCAAACCGAAGTGCTTTTTCCCTGGAAGGGCGGCAAACCAACGCTACGCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCAcaa < 1:942093/239‑1 (MQ=255) cTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTaa > 1:188662/1‑130 (MQ=255) aCCGCAAAACGAAGTGGCTTTTACCTGGAAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcgc < 1:181482/238‑1 (MQ=255) aCCGAAAACCGAGGGGGTTTTTCCTTGGCAGGGGGGCGAACCAACGCTACCCGGGCGGGCTTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCATACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATcgc < 1:93504/238‑1 (MQ=255) | ATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAACAGGTTCCAACGCGTGCTTTTCATGTGATGGCGAAACCGAGCGGTTCCGATTGTAATCTGAACTGTGACTACTGTTTTTATCTCGAAAAACAATCCCTTTACCGCGAAAAGCCAGTCACGCATATGGACGATGACACGCTGGAAGCGTATGTCCGTCACTATATCGCTGCCAGCGAACCGCAAAACGAAGTGGCTTTTACCTGGCAGGGCGGCGAACCAACGCTACTCGGGCTGGCGTTTTACCGCCGTGCCGTAGC‑GCTACAGGCGAAATATGGTGCTGGCAGGAAGATAAGTAACAGCTTCCAGACTAACGGCGTGCTGCTGGATGACGAATGGTGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGC > NC_000913/3982911‑3983380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |