Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	433,649	T→G	35.8%	G65G (GGT→GGG)	ribD →	fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	433,649	0	T	G	35.8%	21.6 / 8.1	47	G65G (GGT→GGG)	ribD	fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
Reads supporting (aligned to +/- strand):  ref base T (9/19);  new base G (13/5);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

AGAATTTGGCGAAGAGATCGC‑GCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGG  >  NC_000913/433415‑433864                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                           aGAATTTGGGGAAGAG‑TCGCGGCGCCTGGAGTACTAAGCCGGCGGGGAAGAGTATTAAAGGGCGGGGGCGCTAAAGCGGGGGCAC‑CGAGGACGTTTCACCACGCACCCCAACCCGAATGG‑CGGGTGCATCATTGTCAAAGATGGCGAAATGGTCGGTGAAGTTTACCACCAA‑CGTGCGGGTGAACCAAATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGa                                                                                                                                                                                                                       <  2:276285/239‑1 (MQ=255)     tttGGCGAAGAGATCGC‑GCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAC‑CAAAAGG‑GGGAccc                                                                                                                                                                                                                    >  1:492089/1‑237 (MQ=255)     tttGGCGAAGAGATCGC‑GCGCCTGGAGGACTAAGCCGTGCAGGAAGAGTTTTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGAGGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑AAAAGGT‑GCGACCGc                                                                                                                                                                                                                   <  1:1032546/239‑1 (MQ=255)       tGGCGAAGAGATCGC‑GCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGGATGGC‑GGGTGGAAAAGC‑CAAAAGG‑GCGCCGGccc                                                                                                                                                                                                                 >  1:772222/1‑238 (MQ=255)        ggCGAAGAGATCGC‑GCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAGAAAGC‑CAAAGGG‑GGGACCGcc                                                                                                                                                                                                                  >  1:963111/1‑237 (MQ=255)                              ggggaCTAAGCCGGGCAGGGAGAGTTTTACAAGGCGCGGGCGCTAAAGCTGGCGCAA‑CGGGGGCGTTTTACCACGCAGCCCAACCCGAATGT‑CGGGTGCGTCATTGTAAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAG‑CGTGGGGGTGAACCAAATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑AAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTa                                                                                                                                                                                           <  1:1241767/236‑1 (MQ=255)                                           tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGC‑CCAAGGT‑GCGACCGCCCATTTCACA‑CTCGAACCCGGTAGCcatccatggcag                                                                                                                                                                             >  1:117070/1‑231 (MQ=255)                                           tGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGC‑CAAAGGG‑GCGACCCCCCATTTCCCA‑CTCGAAACCTGTAGcctgtccctttt                                                                                                                                                                              >  1:376933/1‑228 (MQ=255)                                             cAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGC‑CAAAGGG‑GCGACCCCCCATGTCCCA‑CCCCAACCCTGTAGCcatccatgacgta                                                                                                                                                                            >  1:568770/1‑229 (MQ=255)                                                     gTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGC‑CAAAGGG‑GCGACCGCCTATGTCCAA‑CTCGAAACCTGTAGCCACCATGGTCGTaacgccccc                                                                                                                                                                    >  1:174216/1‑230 (MQ=255)                                                      ttttACATGCCGCGGTCGTTAAAG‑TGGGACAAGCGGGGTCGTTTGACCACGCACCCCACCCCGAATGG‑GGGGTGCGGTATTGACAAAGAGGGAGAAATGGTGGGGGAGGTTTACCACCAC‑GGGGCGGGTAACCCCAAGGCCGAATTCCCGGCGTGGCGTATGGG‑GGGTAAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTg                                                                                                                                                                  <  2:395717/237‑1 (MQ=255)                                                        ttaacaggCGGGGGCGGTAAAGGTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCCCCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCt                                                                                                                                                                <  1:236545/233‑1 (MQ=255)                                                          aCATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGG‑GCGACCGCCCATGTCCCA‑CTCGAACCCTTGAGCCATCATGGCGGTACGCCCCCGTGCtgt                                                                                                                                                              >  1:70454/1‑239 (MQ=255)                                                              ggCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAAC‑CAAAAGG‑GCGACCGCCCATGTCACA‑CTCGCACCCCGTAGCCATACTGGTCGTACGCCACCGGGCTGTGAc                                                                                                                                                           >  1:359011/1‑238 (MQ=255)                                                               gggcgGGCGTAAAAGGTGGCGCAA‑CGGGGCGGTTTTACCACGCATCCAAACCCGAATGG‑CGGGGGCGGCATTGTCAAAGATGGCGAAATTGTCGATGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGAc                                                                                                                                                           <  1:1155546/235‑1 (MQ=255)                                                                cgcgGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGGAAAAGC‑CAAAGGT‑GCGACCGCCCATGTCACA‑CTCGAACCCTGTAGCCACCAGGGTGGTACGCCCCCggcgctggacgc                                                                                                                                                         >  1:19772/1‑227 (MQ=255)                                                                cgcgGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGC‑CAAAAGG‑GGGACCGCCTATTTCCCA‑CTCGGACCCTGTAGCCATCATGGGCGTACGCCACCGTGCCGTGACGc                                                                                                                                                         >  1:368718/1‑238 (MQ=255)                                                                cgcgGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAC‑CAAAGGG‑GCGACCCCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTAAGCCACCGGGCTGGGACGc                                                                                                                                                         >  1:1126619/1‑238 (MQ=255)                                                                  cgGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGG‑GCGACCGCCTATGTCACA‑CTCGAACCCCGAAACCCTCATGGTCGGACCCCCCCCTGCTGTGACGccc                                                                                                                                                       >  1:1324212/1‑236 (MQ=255)                                                                  cgGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAC‑CAAAAGT‑GCGACCCCCCATGTCACA‑CTCGAACCCCGTAGCCATCATGGGCGTACGCCCCCCGCCTGTGCCGCAc                                                                                                                                                       >  1:508101/1‑238 (MQ=255)                                                                  cgGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGATGAAGGTTACCACCAA‑CGTGCGGGGGAACCACATGCCGAAGTACACGCGTTGCGAATGGCGGGGGGAAAAACA‑CAAAGG‑‑GGGACGCCCTATGTCACA‑CCCCAACCCTCTAGCCCCAATGGTCGTACCCCACCCCGCTGTGGCGAAc                                                                                                                                                       >  2:1155546/1‑238 (MQ=255)                                                                                ggCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGGAAAAGC‑CCAAAGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCAAGGGCGTACCCCCCCGTGCTGTGACGCACTCATCCCCCCTGGCg                                                                                                                                        >  1:530131/1‑239 (MQ=255)                                                                                         ggggtcGTTTTCCCCCCCCTCCCACCCCGAATGG‑GGGGGGGAGCATTGCAAAAGAGGGGAAAGTTGCCGGAGAAGTTTACCACCAA‑CGGGGGGGGGAACCACGGGCGGAAGTACCCGCGTTGCGTAGGGG‑GGGTGAAAAAGC‑AAAAGGT‑CCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcgcgc                                                                                                                                <  2:508101/234‑1 (MQ=255)                                                                                                  ttccccccccccccACCCCGAATGG‑GGGGTGCGTAATTG‑CAAAAGTGGGGAAATTTGCGGGTGGGGTTTCCCCCAAGCGGGGGGGGAAACCCCATGCCAAAGCCCACGCTTGGCGTTGGGG‑GGGTAAAAAAGC‑CAAGGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGcc                                                                                                                       <  2:1369922/228‑1 (MQ=255)                                                                                                          ctccccAACCCGATTGG‑GGGGTGGTTCTTTGTCAAAGGTGGCAAATTTGGCGGTGAGGGTTGCCCCCAC‑CGGGCGGGTGACCCACGGGCGGAAGTACGCGCGTTGCGTAGGGC‑GGGAGAAAAAGC‑AAAGGGG‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                               <  2:70454/236‑1 (MQ=255)                                                                                                          caccccAA‑CCGAATGGCCGGGGGCGCAATTGTCAAGAATGGGAAAATTGCCGGTGAAGGTTCCCACCAA‑CGGGCGGGTAAACCCAATGCCGAAGTCACCGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                               <  1:955844/236‑1 (MQ=255)                                                                                                          cATCCAAACCGGAATGG‑CGGGGGCGTCATGGTAAAAGAGGGCGAAATTGTCGGTGAAGGTTACCCCCAA‑CGTGGGGGGAACCCCCATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑AAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                               <  1:753640/239‑1 (MQ=255)                                                                                                                       aTGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATAATGGTGGTACCCCCCCGGGCTGTTACGCACCCCACGCCCCTGGCGTAGCGCGCGTGGGTGCCCCGAGGCAAAGTCCTAAccc                                                                                                   >  1:1175961/1‑238 (MQ=255)                                                                                                                                  cGTCATTGAAAAAGGTGGCGAATTTGTCGGTGAAGGTTACCCCCAA‑GGGGCGGGTAAACCCAATGCCGAAGTACCCGGTTGGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCt                                                                                        <  1:1186953/238‑1 (MQ=255)                                                                                                                                     cATTGAGAAAGAGGGAGAATTTGTCGGTGCGGGTTCCCGCAAA‑GGTGGGGGTGACCCCCACGCCGAGGTCCACGCGTTGCGTAGGGC‑GGGGGAAAAGGC‑CAAGGGG‑GCAACCGCCTAGGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGc                                                                                    <  2:235245/239‑1 (MQ=255)                                                                                                                                        tGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGTAAAACC‑CAAAATG‑GCGACCGCCTATGT‑ACACCTCGAACCCTGTATCCCTCCTTGTTGCACGCCCCCGTGCTGTTACGCACCCCTCTCCCCCTGCGTAGCACGCGTGGTTGCCTCGATGCCCGAACCCAACCCCCAAGG‑CGCGGGGCgcg                                                                                 >  2:166777/1‑237 (MQ=255)                                                                                                                                         gTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAAC‑CAAAAGG‑GCGGCCGCCTTTGTCACA‑CTCGTAACCTTTAGCCATCATTGTCGACCGCCCCCCTGGTGTGACGCCCTCACCGCCGCTTGCGTAGCGCCGGGGGGTGCCCCGGTCAACGACCCTAACCCCCAGGG‑CGCggggggggg                                                                                >  2:1305233/1‑234 (MQ=255)                                                                                                                                                    cGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCCCAGGG‑CGCTGGGCGTGGGCTTTACCg                                                                       >  1:538600/1‑237 (MQ=255)                                                                                                                                                     aaaaTTCGCGGTGAAGGTTACCACCAG‑CGGGCGGGGGAACCCAATGCCGAAGTACCCGCTTTGCGTAGGGG‑GGGTGAAAAGCC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTc                                                                     <  1:1305233/237‑1 (MQ=255)                                                                                                                                                                      ttACCACCAA‑CGGGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCa                                                                                                               <  1:435474/180‑1 (MQ=255)                                                                                                                                                                      gtACCCCCAA‑CGGGGGGGGGAACCACATGCCGAAGTACCCGGGTTGCGTATGGC‑GGGTAAAAAAGC‑AAAAGGT‑GCGACCGCCTATGTCACG‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCAtt                                                    <  1:951299/237‑1 (MQ=255)                                                                                                                                                                                 ggcgggggTGACCCACAGGCCGGAGTACCCGGGGGGCGGATGGG‑GGGTGAAAACGC‑CAGGGGT‑GCGCCCGCCTATGTCACC‑CTCGAACCCTGTAGCCCTCATGGTCGTACGCCACCGTGCTGTGACGCCCTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                         <  2:960969/234‑1 (MQ=255)                                                                                                                                                                                 cgggggggTAAACCCAAGGCCGAAGTCCCCGGGTGGCTTATGGG‑GGGTAAAAAACC‑CAAAGGG‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCa                                         <  1:975689/234‑1 (MQ=255)                                                                                                                                                                                 cgggCGGGTGAACCAAGTGGCGAATGACGGGTTTGGCTTAGGGC‑GGGTAAAAACGC‑AAAAGGT‑GCCACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTAGCATTGACGTCAGCCa                                         <  1:799241/236‑1 (MQ=255)                                                                                                                                                                                      gggTGAACCACAGGCGGAA‑TTCCCGGGTTTGGTGAGGG‑GGGGAAAAAACC‑CAAAGGTGGCAACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGGCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGc                                     <  2:538600/238‑1 (MQ=255)                                                                                                                                                                                        gTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGGGAAAAAGC‑CAAAGGT‑GCGACCGCCCATGTCACA‑CTCGAACCCTGTAACCATCATGGTCGTACGCCACCGGGCTGTGACCCCCTCATCGCCCCCGGCGTAACGCGCGGGGTTGCCCCGGTGCCAGATCCTAACCCCCCGGTACCCGGGGCG‑GGGCTTTACCGTCTGCAACAGGCTGGCCTTGAGGGCAGCCACGGcccg                                  >  2:1117436/1‑237 (MQ=255)                                                                                                                                                                                                           aaccgggTTGCATATGGC‑GGGT‑AAAAAGCACAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAg                <  1:930248/232‑1 (MQ=255)                                                                                                                                                                                                                         tgggggggTAAAAAACC‑CAAAGTG‑CCCACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCCCCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAgg  <  1:105846/234‑1 (MQ=255)                                                                                                                                                                                                                                gTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCt                                                                                                                      >  1:752538/1‑117 (MQ=255)                                                                                                                                                                                                                                gTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCt                                                                                                                      <  2:752538/117‑1 (MQ=255)                                                                                                                                                                                                                                            aaaGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCcatca                                                                                                                                                                                    >  2:247156/1‑44 (MQ=255)                                                                                                                                                                                                                                            aaaGGG‑GGGACCGCCTATGTCACA‑CTCGAACCCTGTAGCcatca                                                                                                                                                                                    <  1:247156/44‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                           AGAATTTGGCGAAGAGATCGC‑GCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAA‑CGAGGACGTTTTACCACGCATCCCAACCCGAATGT‑CGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAA‑CGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGC‑GGGTGAAAAAGC‑CAAAGGT‑GCGACCGCCTATGTCACA‑CTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGT‑CGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGAAGCCGAGCAATTGAATAAAGG  >  NC_000913/433415‑433864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑