BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,638,873	A→T	10.3%	L232F (TTA→TTT)	creD →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,873	0	A	T	10.3%	468.8 / 6.2	160	L232F (TTA→TTT)	creD	inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (64/72); new base T (16/2); total (82/78)
Fisher's exact test for biased strand distribution p-value = 7.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

GGTGTGGTGAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTT  >  NC_000913/4638637‑4639110

ggtgtggtgAAAGCCGCTGAAGTTAACGGAA‑CGGCGCTGACCAGTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAgg                                                                                                                                                                                                                                                <  2:321133/239‑1 (MQ=255)

ggtgtggggAAAGCGCCTGAAGGTAACGGAA‑CGGCGCTGACCGTTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAgg                                                                                                                                                                                                                                                <  1:1320814/239‑1 (MQ=255)

  tgtggtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATTGCCCTGAATTTAAACGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGGGTAAATGCGAAATGACCTTAACCAGCCAC‑CGGCCCCATCCCAGTTTTTTaaggg                                                                                                                                                                                                                                              >  1:608365/1‑235 (MQ=255)

      gtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTTAATATTGCCCTGAATTTTAGCGGGACCGGCGATCTTTTTTGGGTGCCTGGCCGGGGGAATAGCGGAAATACCCTAACCCGCAACTTGGCCGCCCCCCGGTTTTTGGGGGGAtt                                                                                                                                                                                                                                           >  2:1307159/1‑239 (MQ=255)

      gtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTTTGGTGCCTGGCGGGCGCAATAGCGAAATGGCCCTCACCAGCAAC‑CGGCCCCCCCCCCGTGTTTTTGGGGAttt                                                                                                                                                                                                                                          >  2:162730/1‑239 (MQ=255)

      gtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCCGGTTTTTTGGGGAttt                                                                                                                                                                                                                                          >  1:421866/1‑239 (MQ=255)

      gtgAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑GTGCCGCATCCCAGTTTTTTAGGTGtttt                                                                                                                                                                                                                                          >  1:658775/1‑239 (MQ=255)

           aGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGGAGCAGAACCTGAAGCTGAAAATGGCCCTGAATTTAAGCGGTAACGGCGAACCTTTTGTGGTGCCCGGCCGGCTGAAAAGGGAAAAGACCTTTACCACCCAC‑GGGCCGCCTGCCCGGTTTTTTGGGGGTTTTTTA‑c                                                                                                                                                                                                                                    >  2:716466/1‑239 (MQ=255)

           aGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑c                                                                                                                                                                                                                                    <  1:388180/239‑1 (MQ=255)

           aGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑c                                                                                                                                                                                                                                    <  1:268745/239‑1 (MQ=255)

               ccTTAAGTTAACGGGA‑CGGGGGCGACCATTGAACCCGGCCCCGGGTTAGAGCAAGGGGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGc                                                                                                                                                                                                                                <  2:685323/239‑1 (MQ=255)

               ccTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAAACCGCAAC‑TGGCCGCATCCCAGGTTTTTAGGGGGTTTTCTA‑CCaa                                                                                                                                                                                                                                 >  1:1055724/1‑237 (MQ=255)

               ccTGAAGTTAACCGAAGCGCCGTTG‑CCATTGAACCCGGGCCCGGGTTAGGGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGc                                                                                                                                                                                                                                <  2:421866/239‑1 (MQ=255)

                 tggatttAACGGAA‑AGGCGGTGACCATTGAACCCGGCCCCGGGTTAGAGCAAGGCTGGCAGGGCCTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAAGCTGAAGCTTAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCa                                                                                                                                                                                                                              <  1:50352/234‑1 (MQ=255)

                 tGAAGGTAACGTGA‑CGGGGCGGCCCATTGAACCCGGAACCGGGTTAGGGCAAGGCGGGCAGGGCGGTCATATCCCTTTTCCTGAAGGGGACGGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAAGTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTGCTA‑CCAGCCa                                                                                                                                                                                                                              <  2:1107543/239‑1 (MQ=255)

                         acggaa‑cggCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGGAGCTGAATATGGCCCTGAATTTAACCGGTACCGGGGATCTTTCTTTGGGGCCCTGCCGGCGTGAAAGCGCAATGACCTTTACCCGCAAA‑CGGCCGCCCCCCCGGTTTTTTGGGGGTTTTTTACCCAGCAAAACgggg                                                                                                                                                                                                                       >  2:1245914/1‑238 (MQ=255)

                                        aCCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGGACCGGCGATCTTTCTGTGGTGCCTGGCGGGGGTAAAAACGAAAAGACCTTAACCAGCAAC‑TGGCCCCATCCCAGTTTTTTAGGTGATTTTTTC‑CCAGCCAAAAGGGGA‑GATAAGGAGACAgg                                                                                                                                                                                                       >  2:246305/1‑239 (MQ=255)

                                          cATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAg                                                                                                                                                                                                            <  1:1102213/232‑1 (MQ=255)

                                                   tggCCCCGGGGTAGAGCTAGGGGGGCGGGGCGTGTATTTCCCTTTCACTGGAGGGGACTGGCGGAAGCATAACCTGAAGCCGAATATGGCCCGGGATTTAAGCGGGACCGGCGATCTTTCTGGGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGGTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGgcgc                                                                                                                                                                                            <  1:146441/238‑1 (MQ=255)

                                                   cggacctgggTTAGAGGAGGGTGGGCAGGGTGTGCATATCCCGTTTCCTGAAAGGGACTGGGGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGGGTGGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGgcgc                                                                                                                                                                                            <  2:339302/232‑1 (MQ=255)

                                                   cGGCACCGGGTTAGAGCAAGGCGGGAAGGGCGTGCATATCCCTTTCCCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCCGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGgcgc                                                                                                                                                                                            <  1:773119/239‑1 (MQ=255)

                                                    ggCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGGAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCCGTTTTTTAGGGGATTTTCTA‑CCCGCCAAACGGGAA‑GGTAGCGAGTCAGGTTTTCAGgcgc                                                                                                                                                                                            >  1:924053/1‑238 (MQ=255)

                                                    cgccccGGGTTGGAGGAAGGCGGGCAGGGCGGGCATAGCCATTTACCCGAAGGGGGCTGGGGGAAGCAAAACCTGAAGCTGAATATGGCCCTGAATTTAAGCTGTACCGGCGATCTTTCTGTGGTGGCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAA‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCa                                                                                                                                                                                           <  2:1189699/235‑1 (MQ=255)

                                                          gggTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCa                                                                                                                                                                                     <  1:1277048/239‑1 (MQ=255)

                                                               agggCAAGGCGGGCAGGGCGGGAATATCCCTTTGCCGGAAGGGGACTGGCGGAAGCAGAAACTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAagc                                                                                                                                                                                 <  1:1289309/235‑1 (MQ=255)

                                                                     aGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAAAGGGAG‑AGTACCGAGGCCGGGTTTTCGGGGCCCCGGCAAAGCCGCCggg                                                                                                                                                                          >  2:26424/1‑238 (MQ=255)

                                                                      ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGTATATGGACCTGAGTTTTAACGGTACCGGCGACCTTTCTGTGGTGCCTGGCGGGGCTGATATAGAAATTGCCTTAACCCGCAAC‑TGGCCGCGCGCCCGGTTTTTAAGTGAATTCTTA‑CCACCCAAACGGGAG‑GTTATAGGGTCAGGCTTTTCGTCGCACTCGCAAagcaccggggt                                                                                                                                                                         >  2:827299/1‑232 (MQ=255)

                                                                      ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTTTGTGGTGCCTGGCGGGCGGAATAACGAAAGAGCCTTAACCAGCAAC‑TTGCCCCCTCCCAGGTTTTTTGGTGATTTTTTA‑CCAACCAAAAGGGGA‑GGTAGCGAGTCGGGGTTTTTTGGGCACTGGCAAAACAACTgggt                                                                                                                                                                         >  2:1094443/1‑237 (MQ=255)

                                                                      ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTTGCGGGCGTAATAGCGAAATGACCTTAACCAGCCAC‑TGGCCGCATCCCCGTTTTTTTGGCGGTTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGGTTTCCGGGGCACTGGCCAAACAGCGGGtt                                                                                                                                                                         >  1:638111/1‑239 (MQ=255)

                                                                      ggCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGGTTTCAGCCGCACTGGCAAAGCAGCCgggt                                                                                                                                                                         >  1:111811/1‑237 (MQ=255)

                                                                           gCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAGCCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCt                                                                                                                                                                     <  1:1039746/238‑1 (MQ=255)

                                                                             aGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCtaa                                                                                                                                                                   <  1:207921/238‑1 (MQ=255)

                                                                              gggCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGGGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCACCAAC‑TGGCCGCATCCCCGTTTTTTTGGGGATTTTCTA‑CCCGCCAAACGGGAAGGTTAGCG‑GGCAGGTTTTTAGGGGCACCGGCAAAACAGCAGGTTTgtgcaaa                                                                                                                                                                 >  2:203813/1‑233 (MQ=255)

                                                                                       tatCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTg                                                                                                                                                        <  1:300029/239‑1 (MQ=255)

                                                                                        atCCCTTTACCGGAAGGGGACTGGCGGAAGCAGAACCTGAATCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGa                                                                                                                                                       <  1:1085648/239‑1 (MQ=255)

                                                                                         tCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGGCTCCCAGTTTTTTAGGGGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTACGGCGCCCTGGCAAAGCCGCCGGTTTGCGAATAATCTCGGTGAg                                                                                                                                                      >  1:155269/1‑239 (MQ=255)

                                                                                              ttACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGGGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAACGAGTCCGGTTTTCAGGGGCCCTGGCAAACAAGCTGGTTTGTTAATAATCCCGGGTAGGGttt                                                                                                                                                 >  2:153449/1‑239 (MQ=255)

                                                                                               tACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGtttt                                                                                                                                                >  1:1009372/1‑239 (MQ=255)

                                                                                               tACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGGGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTTGCAAACGAACTGGTTTGCTAATAATCTCGGTGAGCGtttt                                                                                                                                                >  1:1250308/1‑239 (MQ=255)

                                                                                                   tGAAGGGGGCTGGCGGAAGGAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCCATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCtt                                                                                                                                            <  2:940361/239‑1 (MQ=255)

                                                                                                   ggAAGGGGAGGGGCGGAAAGAGAACTGGAAGCTGAAATGGGGCCGTAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGGGGGCGTATGAGCGAAATGACCTTAACCCGCAAG‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCTCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCtt                                                                                                                                            <  2:783337/238‑1 (MQ=255)

                                                                                                    gAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TAGCCGCATCCCCGTTTTTTTGGTGATTTTCTA‑CCAGCCAAAAGGGCA‑GTTACCGAGTCAGGGTTTCAGGCGCCCGGGCCAAACAGCTGGTTTGCTTATAATATCGGTGACGGTTTTGTTTc                                                                                                                                           >  2:1056974/1‑239 (MQ=255)

                                                                                                     aaGGGGACTGGCGGAAGCAGACACTGAAGCTGAATTGGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCa                                                                                                                                          <  1:248108/239‑1 (MQ=255)

                                                                                                     aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATTGCCCTGAATTTAAGCGGTACCGGAGATCTTTCTGTGGTGCCTGGCGGGGGTAATAGCGAAATGACCTTAAACAGCAAC‑CGGCCGCATCCCCATTTCTTAGGGGATTTTCTA‑CCCGCCCAACGGGCA‑GTTATCGGGTCAGGGTTTCCGGCGCACCGGCAAAACACCCGGGTTGCCACAAATTCCGGGGGGGGTTTttcttcc                                                                                                                                          >  2:994559/1‑238 (MQ=255)

                                                                                                     aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGGGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGAAAAAAAAGCGGGTTGTTAAAAATCTCGGGGAGCGGTTTGCTTCa                                                                                                                                          >  2:291706/1‑239 (MQ=255)

                                                                                                     aaGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGGAATAGCGAAATGACCTTAACCAGCAAA‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTTTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCCGGTTTTCAGGGCCACCGGCAAAGGAGCCGGTTTTCTAAAAATTCCGGTGGGGGgtttgtttaa                                                                                                                                          >  2:1031020/1‑237 (MQ=255)

                                                                                                      aGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCa                                                                                                                                          <  1:221098/238‑1 (MQ=255)

                                                                                                           aCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGtt                                                                                                                                                                         >  1:1015860/1‑202 (MQ=255)

                                                                                                           aCTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGtt                                                                                                                                                                         <  2:1015860/202‑1 (MQ=255)

                                                                                                               gCGGAAGCAGCACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGCTCTTTCTGTGGTGCCTCCCGGGCGTAATAGCGCAATGACCTTAACCAGCAAC‑CGGCGGCATCCCAGTTTTTTTGGTGTTTTTCTC‑CCAGCCAAACGGGCA‑GTTAGCGAGTCAGGTTTTCACGCGCACCGGCAAAGCAGCTGGCTTGCTTATAATCTCGTCGACCGCTTCGCTTCatgacatcgt                                                                                                                                 >  2:307516/1‑229 (MQ=255)

                                                                                                                         aaCCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGTAA‑GTTAGCGAGTCCGGTTTTCAGGCGCACTGGCAAACCAGCGGGTTTGCTAATAATCTCCGGGAGGCGTTTGCTTCTGGCAATGATACCGGCTGGGa                                                                                                                      >  2:410449/1‑239 (MQ=255)

                                                                                                                         aaCCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGACCGTTTTGCTTCAGGCAATGATACCGGCTGGGa                                                                                                                      >  1:610348/1‑239 (MQ=255)

                                                                                                                                   tGAATATGGCCCTGAATTTAAGCGGTACCGGGGATCTTTCTGTGGTTCCTGGCGGGCGTAATCGCGAAATGACCTTAACCAGCAAC‑TGGCCGCAACCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCg                                                                                                            <  1:1366572/239‑1 (MQ=255)

                                                                                                                                   tGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCCGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGGTTTCTAATAATCTCGGTGAGCGTTTTGATTAAGGCAATGCTACCGCCTGGGGAAACTTctcc                                                                                                            >  2:837477/1‑236 (MQ=255)

                                                                                                                                   tGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTcc                                                                                                              <  1:818321/237‑1 (MQ=255)

                                                                                                                                   tGAATAGGGCCCTGAATTTAAGCGGTTCCGGCGATCTTTCTGGGGTGCGTGGAGGGCGTAATAGCGAAATGACCTTAACCAGGAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTcc                                                                                                              <  2:1250137/237‑1 (MQ=255)

                                                                                                                                     aaTATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCCGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCCCTGGCAAAGCAGCTGGTTTGCTAATCATCCCGGTGCGCGTTTTGCTTCAGGACATGAACCCGGCTGGGAAAACATCCCGGc                                                                                                          >  2:1081710/1‑239 (MQ=255)

                                                                                                                                              cTGAATTTAAGCGGGACCGGCGATCGTGCTGTGGGGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAAGCACGGTGAGCGTTTTGCTTCAGGCAAGGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGt                                                                                                 <  1:220095/239‑1 (MQ=255)

                                                                                                                                               tGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCgg                                                                                                 >  1:868119/1‑237 (MQ=255)

                                                                                                                                               tGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGt                                                                                                 >  1:1189664/1‑238 (MQ=255)

                                                                                                                                               tGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGCCAATGATACAGGCTGGGAAAACTTCCCGGCGTTTAGAGTc                                                                                                >  1:189022/1‑239 (MQ=255)

                                                                                                                                                 aaTTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAACCGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCCGGCAAGAGTACCGGCTGGGGAAAATTCCCCGCGGTTAGCGTCga                                                                                              >  2:386445/1‑238 (MQ=255)

                                                                                                                                                  aTTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCC‑AACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCa                                                                                             <  1:716466/238‑1 (MQ=255)

                                                                                                                                                      aaGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTACCGGCGTTTAGCGTCGccggaa                                                                                         >  2:1060750/1‑234 (MQ=255)

                                                                                                                                                               cGGCGTTTTTTCTGGGGTGCCTGGGGGGCGAAATAGCAAAAGGACCTTACCCAGCAAC‑GGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTGGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAg                                                                               <  2:1009372/239‑1 (MQ=255)

                                                                                                                                                                ggCGATCTTTCTGGGGTGCCTGGCGGGCGTAATAGCGAAATGCCCTTAACCAGCAAC‑TGGCCGGATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCTCAGTAACGA‑CGCCAGc                                                                              <  1:985276/239‑1 (MQ=255)

                                                                                                                                                                   gtttgttCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGAAAA‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCGCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCg                                                                            <  2:640434/233‑1 (MQ=255)

                                                                                                                                                                      cTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAAACTCGGGGAGCGTTTTGCTTCAGTCACTGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCCGTa                                                                                          >  1:1097955/1‑222 (MQ=255)

                                                                                                                                                                       tttttttggtgCCTGGCGGGCTTAAAAGGGAAATTCCCTTAACCAGAAAC‑GGGCCGCTTCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTGGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCaa                                                                       <  2:619547/233‑1 (MQ=255)

                                                                                                                                                                       tttCTGTTGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCaa                                                                       <  1:569641/239‑1 (MQ=255)

                                                                                                                                                                       tttCGGGGGTGCCTGGCGGGAGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCGGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCaa                                                                       <  1:821391/239‑1 (MQ=255)

                                                                                                                                                                        ttCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCCGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAAt                                                                      <  1:1296450/239‑1 (MQ=255)

                                                                                                                                                                            tgtgtgCCGGGGGGGGGTAATAGGCAAATTACCGTAACCCGCAAC‑TGGCCGGATACCAGTTGTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGGGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCa                                                                  <  2:511270/236‑1 (MQ=255)

                                                                                                                                                                              ggtgCCTGGCGGGCGTAATAGCGAGATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAAt                                                                >  1:515504/1‑239 (MQ=255)

                                                                                                                                                                               gtgCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAAtt                                                               >  1:979557/1‑239 (MQ=255)

                                                                                                                                                                                tgCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGTTCAATACCAATTa                                                              >  1:583706/1‑239 (MQ=255)

                                                                                                                                                                                 gCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCaaa                                                                                                                                                                                                                            <  1:510151/82‑1 (MQ=255)

                                                                                                                                                                                 gCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCaaa                                                                                                                                                                                                                            >  2:510151/1‑82 (MQ=255)

                                                                                                                                                                                 gCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTTGGAAAACTTCCCGGCGTTTAGCGTCGCCGTAACGA‑CGCCAGCCGCTCCATACCAATTa                                                              >  1:455643/1‑238 (MQ=255)

                                                                                                                                                                                  ccTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGGAGTAACGG‑CGCCAGCCGATCAATACCAATTAAc                                                            >  1:770034/1‑239 (MQ=255)

                                                                                                                                                                                  ccTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGCCGTTTAGCGTGCCCGTAAAGA‑CGCCAGCCGATCAATACCAATTa                                                              >  1:188969/1‑237 (MQ=255)

                                                                                                                                                                                  ccTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGC‑GAGACAAGCGCCACCCGATCAAAACCAATTaaa                                                            >  2:57586/1‑238 (MQ=255)

                                                                                                                                                                                        gggggTAATTGGGAAATGCCCTTAACCAGCAAT‑TGGCCGGATCCCGGTTTTTTGGGTGATTTTTTA‑CCAGCCAAACGGGAA‑TTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCGGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGAcc                                                       <  2:298200/238‑1 (MQ=255)

                                                                                                                                                                                             ttattcgctaaTGCCCTTAACCCGCAAT‑TGGGCGCATTCCAGTTTTTTTGGTGATTTTCCA‑CGAGCCAAACGGGAA‑GTTAGGCAGTCGGGGTTTCAGGCTCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGa                                                 <  2:128618/230‑1 (MQ=255)

                                                                                                                                                                                               aTAGGGAAATGACCTTACTCATGATC‑TGGCCGCTTCCCATGTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTGGCGAGTCAGGTTTTCAGGCGCACTGGAAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACt                                               <  2:608365/239‑1 (MQ=255)

                                                                                                                                                                                                      aattgccTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:707991/234‑1 (MQ=255)

                                                                                                                                                                                                      aaTGCCCTTAACCAGAAAA‑TGGCCGCAACCCAGTTTTTTGGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTGTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:1239981/239‑1 (MQ=255)

                                                                                                                                                                                                      aaTGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:153449/239‑1 (MQ=255)

                                                                                                                                                                                                      aaTGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCCCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:1209955/239‑1 (MQ=255)

                                                                                                                                                                                                        ttgccTTAACCCGAAAC‑GGGCCCAATCCCGTTTTTTGAGGTTATTTCCCA‑CCAGCCAAAGGGTAA‑TTTAGCGAGACAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCCCGGTGAGCGTTTTGCTTCAGGAAATGATCCCGGCTGGAAAAACTGCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGcc                                      <  2:135826/236‑1 (MQ=255)

                                                                                                                                                                                                           ccTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACg                                        <  1:1067014/234‑1 (MQ=255)

                                                                                                                                                                                                            cTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTTGCAGTAACGA‑TGCCCGCCGATCACTACCAATTAACTGACCGGGCGGCCAAGTCCGCCac                                    >  2:1209189/1‑236 (MQ=255)

                                                                                                                                                                                                              tAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTct                                 >  1:135826/1‑238 (MQ=255)

                                                                                                                                                                                                                       aC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  1:57586/239‑1 (MQ=255)

                                                                                                                                                                                                                       aC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  1:1060750/239‑1 (MQ=255)

                                                                                                                                                                                                                       aC‑TGGCCGCATCCCAGTTGTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAGCTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  1:638280/239‑1 (MQ=255)

                                                                                                                                                                                                                        tttGGCCCCCTCCTCGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGTGTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCa                       <  2:1113466/237‑1 (MQ=255)

                                                                                                                                                                                                                          tGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACt                     <  1:246305/239‑1 (MQ=255)

                                                                                                                                                                                                                                cATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGtt                                                                                                                                                  <  1:313609/109‑1 (MQ=255)

                                                                                                                                                                                                                                             tAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCCGTAACGA‑CGCCAGCCGAGCAATACCCATTAACTGACCGGGCGGCCAAGGACCCCATCCTGCCGATTGCCCTGACTTTTTTGGGGTTCttt  >  2:953176/1‑239 (MQ=255)

GGTGTGGTGAAAGCGCCTGAAGTTAACGGAA‑CGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAAC‑TGGCCGCATCCCAGTTTTTTAGGTGATTTTCTA‑CCAGCCAAACGGGAA‑GTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGA‑CGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTT  >  NC_000913/4638637‑4639110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`