Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	758,596	G→C	17.0%	intergenic (+191/‑110)	sdhB → / → sucA	succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	758,596	0	G	C	17.0%	132.5 / 3.4	61	intergenic (+191/‑110)	sdhB/sucA	succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (32/16);  new base C (7/4);  total (41/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

TCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTG  >  NC_000913/758379‑758827                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           tCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑GAGCCACTCGGTAGACaaaa                                                                                                                                                                                                                      >  1:705661/1‑237 (MQ=255)  gtttttttttgCAGCGGAATGCCGAACCCGTAGGCCTGAAATCGCGCGCAAGCG‑CCCAAGAGAGCACACGGTGCCGGACGGGGGGTGCACGCCTT‑TCCGGGCTAAAGAGTCATCCCGCGGAGGCCAGAAAAGCGCAGCGCATCAGGAGTAAAAAAAAAATGCAGAAATTCTTAAAAAACG‑CCCCCG‑GACACAAAGACAGTTTTAAA‑AGTTTC‑CT‑TCGC‑GAGCCACTACGTAGACAagag                                                                                                                                                                                                                     <  2:361828/231‑1 (MQ=255)     tgttgtTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCt                                                                                                                                                                                                                   >  1:264557/1‑238 (MQ=255)     tgttgtTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GAAACTAAGACAGTTTTTAA‑AAGTTC‑CT‑TCCC‑GATCCACTACGGAGACAAGACCt                                                                                                                                                                                                                   >  1:501953/1‑238 (MQ=255)     tgttgtTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCCCATCAGGCGTAACAACGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GCAACCACAACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACCACGTAAACAAAACCTc                                                                                                                                                                                                                  >  2:843353/1‑239 (MQ=38)                     tGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCCTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGCCAAGACCTCGCAAGTGAACCCCgc                                                                                                                                                                                                   >  1:188212/1‑237 (MQ=255)                            aCCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGACCGCCTTACCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GCAACCAAGACAGTTTTTAA‑AGGTTC‑CT‑TCAC‑GAGCCACCACGCAAACAAGAGACCGCAAGGGAACCCCGgcccgcca                                                                                                                                                                                            >  2:511106/1‑236 (MQ=38)                                tAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAAAGCTCGCAAGTGAACCCCGGCCCGcac‑aac                                                                                                                                                                                        >  1:755138/1‑235 (MQ=255)                                tAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑TGGCCCCTACGTAGACAAAAGCTCGCAAAGGAACCCCGGCACGCCC‑ATc                                                                                                                                                                                        >  1:1195097/1‑237 (MQ=39)                                 aGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAAAGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑GAGCCACCACGTAAAAAAAAACCCGCAAGAGAAACCCCGcacacac‑acc                                                                                                                                                                                        >  2:442090/1‑234 (MQ=38)                                   gcaggATAAGCCCGGCAGGCGCCGCACAGG‑GCAACCGGGCCCGGATGCGGCGGAAACGCCTTACCCGGCTTAAA‑AGTCATTCCCCGTGGGCTTGATAAGCGCAGCGCATCAGGCTTAACAAAAAAAGGCAGAAAATTTTAAAAAACT‑CCCCTT‑GACACTAAGAAATTTTTAAA‑AGTTCC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACt                                                                                                                                                                                     <  1:489694/233‑1 (MQ=255)                                        aTAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCCC‑GAGCCACTACGTAGACAAAAGCTTGCAAGTGAACACCGGCACGCCC‑ATCCCTGTGctgg                                                                                                                                                                              >  1:1228994/1‑236 (MQ=255)                                        aTAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AAGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCCCAATTGAACCCCGGCACGACC‑ACCACTGCGCGTg                                                                                                                                                                              >  1:1231103/1‑239 (MQ=255)                                        aTAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AACTTC‑CT‑TCCC‑GAGCCACCCCGGCGCCCAGAGCTCGCAAACAAACCCCGGCACACAA‑ATCCCtgtggggg                                                                                                                                                                              >  2:77980/1‑235 (MQ=255)                                                 gcAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAAAGCTCGCAAGTGAACCCCGGCAC‑CACAACCACTGTGCGTGGGAGAATcc                                                                                                                                                                     >  2:1096411/1‑239 (MQ=255)                                                        tCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCCCTGTGCGTGGTAGTATCCACGGCGa                                                                                                                                                              >  1:701172/1‑239 (MQ=255)                                                                g‑gCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCCCGCCC‑ATCACTGTGCGTGGTAGTATCCACGGCCAAGTAAGCa                                                                                                                                                      >  1:294404/1‑239 (MQ=255)                                                                   cAACCGGGGCCGGATGGGGCGTAAACGCCTTAGCCGGCCTAAA‑AGTAATTCCCGGTAGCCTTAAAAGGCGCAGCGCATCAGGCGTAAAAAAAAAATGCAGAAATTCTTTAAAAACT‑GCCCCG‑GACACAAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCAt                                                                                                                                                     <  1:1096411/238‑1 (MQ=255)                                                                    cttgtaggCCGGATGCGGCGTGAACCCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GAAACTAAGACAGTTTTTAA‑AGATTC‑CT‑TCCC‑GAGCCACTACGTAGACAAGAGCTCGCAAAGTAAACCCGGCCCGCCC‑CTCACTTCGCGTGTAAGTATCCCAGGCCAAGAAACCATaa                                                                                                                                                   >  1:533170/8‑239 (MQ=255)                                                                          tgcgggtgggggcgTAACCCCCTTACCCGCCTAAAA‑AGTAATTCCCTGTAGGCTGGAAAGGCCCACCGCATCAGGCATAACAAAGAAATGCAGAAATTCTTAAAAACCT‑CCCCCT‑GACACTAAGACAGTTTTAAA‑GGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAg                                                                                                                                              <  1:820590/228‑1 (MQ=255)                                                                          tGCCGGATGGGGCGTGAACGCTTTACCCGGCCTACA‑ATTAATTACCCGTGGGTCTGATAAGCCCAGCCCATCAGGCGTAAAAAAGAAATGCAGGAAATCTTTAAAACCT‑GCCCCT‑GACCCTAAGACATTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAg                                                                                                                                              <  1:442090/238‑1 (MQ=255)                                                                          tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTTCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGa                                                                                                                                             >  1:267202/1‑239 (MQ=255)                                                                           gCCGGTGGCGGCTTAACCGCCTTACCCGGCCTACA‑ATTCATTCCCCGTAGGCCTAAAAAGCCCAGCGCATCAGGCGTAACAAAGAAATGCAGAAAATCTTAAAAACCT‑CCCCCT‑GACACTAAGACAGTTTTTAA‑AGTTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGa                                                                                                                                             <  1:384885/238‑1 (MQ=255)                                                                                         gAACGCTTAACCCGGACTAAA‑AGTCAATCCCGGTAGGCCTGAAAAGACCAGCCCACCAGGCATAAAAAAAAAATGAAGGAATTTTTTAAAACCG‑GCCCCT‑GACACAAAGACATTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCa                                                                                                                               <  1:671546/238‑1 (MQ=255)                                                                                         gAACGCCTTCACCGCGCTACA‑AGTCATTACCGGTGGGCCTGAAAAGCGCAGCGCATGGGGCGTAAAAAAGAAATGCAGAAAATTTTAAAAAACT‑CCCCCA‑GACAATAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCa                                                                                                                               <  1:1285846/238‑1 (MQ=255)                                                                                             cttttctgcGGCCTAAA‑AGTGATTCCCCTGGGGCCGAAAAAGCGCACCCAAGCAGTAGTAAAAAAAAAGTGCAGGAACTTTTAAAAACCT‑GCCCCT‑GACACTAAGGCATTTTTTAA‑GGGTTC‑CG‑TCCG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATg                                                                                                                          <  2:501953/231‑1 (MQ=255)                                                                                                        ccTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCCC‑ATCACTGTGCGTGGTAGTATCAACGGCGAAGTAAGAATAAACAAGATGCTTAAGGGAGCAAAATGCAGaaaaaggc                                                                                                               >  2:587820/1‑233 (MQ=255)                                                                                                                   aTTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATACACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATAACGATGCAGAACAGGGCTTTGAAAg                                                                                                       >  1:331085/1‑237 (MQ=255)                                                                                                                     tACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAAATGCTTAAGGGATCACGAGGCCAAACAGCGCTTTGAAAACCTg                                                                                                   >  2:146883/1‑239 (MQ=255)                                                                                                                       cccGTAGGCCTGATAAGCGCAGCGCATAAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑AT‑TCGC‑GCGCCACTACGTAGACAAGAGCGCGCAAGTGAACACCGGCACGCAC‑ATCACAGTGCGTGGTAGTATACACGGCGAAGTAAGCATAAAAGAGAAACTTAAGGGAGCAATATACAGAAAAGACCTTTaaaaacgtgtg                                                                                                 >  2:765613/1‑232 (MQ=255)                                                                                                                            gggCTTGAAAAGCGCAGCGCATGAGGCATAAAAAAAAAAGGCAGAAAATCTTTAAAAACT‑GCCCGC‑GACACAAAGCCGGTTTTTAA‑AGTTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGa                                                                                             <  1:843353/237‑1 (MQ=255)                                                                                                                            aGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAAAAGCGCTTTGAAAGCCTGGTTgg                                                                                              >  1:638896/1‑237 (MQ=255)                                                                                                                                        cgcagcgcaTCACGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCAGGTACGCAC‑CTCACTGTGCGTGGTAGAATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGAGTCAGAACAGCGATTTGCAAGCCAGGGTGGA‑CCCTTTTTACct                                                                                >  2:242758/1‑238 (MQ=255)                                                                                                                                          cagcgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGCCAAGAGCTCGCAAGTGAACCCCGGCACGAAC‑ATCACTGTGCGTGGTAGTATCCCAGGCGAAGTAAGCATAACAAAGACGCTTAAGGGATCACGATTCAGAAAAGCCATTTGAAAGCCTGCTTGGA‑CCCTTCTTACctc                                                                               >  2:889236/1‑237 (MQ=255)                                                                                                                                             cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑TTCTTCTTACCTCTCTg                                                                           >  1:1222631/1‑238 (MQ=255)                                                                                                                                             cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTg                                                                           >  1:990563/1‑238 (MQ=255)                                                                                                                                             cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCAACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGAAGCAGAACAGCGATTTGAAAGCCTGGTTGGA‑ACCTTCTTACATCTCTgg                                                                          >  2:484839/1‑239 (MQ=255)                                                                                                                                             cgcaTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGGGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAAAAGCGCCTTTAAAGCCTGGTTGGA‑CTCTTCTTAccccccggg                                                                          >  2:1236632/1‑232 (MQ=255)                                                                                                                                                  cAGGCGTAACAAAGAAATGCAGGAAATCTTTACAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGACAACAAGACCTCGCAATTGAACCCCGGCACGCAG‑ATCACTGTGCGTGGAAGGATCCACGGACAAGTAAGCATAAAAAAGACGCTTAGGGGATCACGCTGCCGAACAGCGCTTTGAAAACCTGGTTGGACCTCTT‑TTACctctcttggcgaa                                                                     >  2:1006298/1‑232 (MQ=255)                                                                                                                                                  cAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCAC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGACGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTAcccccctatgcg                                                                       >  1:435204/1‑227 (MQ=255)                                                                                                                                                       gTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCag                                                                >  1:1035497/1‑239 (MQ=255)                                                                                                                                                         aaaaaaGAAATGCAGAAAATCTTTAAAACCT‑GCCCCT‑GACACTAAGACAGTTTTAAA‑AGGTCC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCagag                                                              <  1:146883/236‑1 (MQ=255)                                                                                                                                                          aCAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTGTTCTTACCTCCCTGGCGCAAACCAGAGc                                                             >  1:490901/1‑239 (MQ=255)                                                                                                                                                          aCAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGCTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCaga                                                               >  1:701116/1‑237 (MQ=255)                                                                                                                                                                   tGCAAGAATTTTTAAAAACCG‑GCCCCT‑AACACAAAGA‑AGTTTTGAACAGTTTC‑CT‑CACT‑GAGCCACTACGTAGACAAGATGTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGaa                                                    <  1:649009/239‑1 (MQ=255)                                                                                                                                                                    tgagaaaaaCTTTAAAACCTCGCCCCG‑GACAAGAAGAACGTTTTAAA‑GGGTCC‑CT‑GCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGaa                                                    <  1:1084169/234‑1 (MQ=255)                                                                                                                                                                               atAAAACCT‑GCCCCT‑GAAACTAGGCATGTTTTTAA‑AGTTCC‑CT‑TCGG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGaa                                        <  1:119615/238‑1 (MQ=255)                                                                                                                                                                                aaaaaaCT‑CCCCCTGGACACAAAG‑CTGGTTTAAA‑AAGGTC‑CT‑TCCCGGGGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGaa                                        <  2:267202/238‑1 (MQ=255)                                                                                                                                                                                                     tAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCtt                     >  1:1375295/1‑237 (MQ=255)                                                                                                                                                                                                            tttttaAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAAc                             <  1:950060/222‑1 (MQ=255)                                                                                                                                                                                                            gTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAAc                             >  2:950060/1‑223 (MQ=255)                                                                                                                                                                                                            cattttAA‑AGGGTCTCT‑GCCG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGt              <  1:729032/237‑1 (MQ=255)                                                                                                                                                                                                             tttttAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAAAAGCTCTATGAAGACTTCTTAAACAAACCTGACTCGGTTg            >  2:715780/1‑239 (MQ=255)                                                                                                                                                                                                             tttttAA‑AGGTTC‑CT‑TCGC‑GAGCCACAACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTg            >  1:247729/1‑239 (MQ=255)                                                                                                                                                                                                             gtttata‑aGGTTC‑CTGCCGG‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGGGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGtt             <  1:587820/233‑1 (MQ=255)                                                                                                                                                                                                               tttAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAACGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGAACCTGAATCGGGTGAc          >  2:76634/1‑239 (MQ=255)                                                                                                                                                                                                                        ttC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATa                                                                                                                                                    <  2:238215/94‑1 (MQ=255)                                                                                                                                                                                                                        ttC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATa                                                                                                                                                    >  1:238215/1‑94 (MQ=255)                                                                                                                                                                                                                        ttC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTg  >  1:1308989/1‑239 (MQ=255)                                                                                                                                                                                                                            cT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAAc                             <  1:155472/209‑1 (MQ=255)                                                                                                                                                                                                                            cT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAAACTTCTTAAc                             >  2:155472/1‑209 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           TCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAG‑GCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACA‑AGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACT‑GCCCCT‑GACACTAAGACAGTTTTTAA‑AGGTTC‑CT‑TCGC‑GAGCCACTACGTAGACAAGAGCTCGCAAGTGAACCCCGGCACGCAC‑ATCACTGTGCGTGGTAGTATCCACGGCGAAGTAAGCATAAAAAAGATGCTTAAGGGATCACGATGCAGAACAGCGCTTTGAAAGCCTGGTTGGA‑CTCTTCTTACCTCTCTGGCGCAAACCAGAGCTGGATAGAACAGCTCTATGAAGACTTCTTAACCGATCCTGACTCGGTTGACGCTAACTG  >  NC_000913/758379‑758827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑