Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,398,908 | A→G | 17.7% | A357A (GCT→GCC) | mreC ← | cell wall structural complex MreBCD transmembrane component MreC |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,398,908 | 0 | A | G | 17.7% | 152.9 / 4.4 | 78 | A357A (GCT→GCC) | mreC | cell wall structural complex MreBCD transmembrane component MreC |
Reads supporting (aligned to +/- strand): ref base A (19/40); new base G (12/4); total (34/44) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.47e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAT > NC_000913/3398676‑3399141 | accAGTATGGCCCCCATCACAAAACCTGGGCCCACCTTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGcac < 1:1085106/236‑1 (MQ=255) gTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGTTGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATGGCTCGCCCCTATTGCCCTCCCGGCGCACGGCAAGGC‑GGTTAGGAAACA‑Ct‑tt > 1:405424/1‑239 (MQ=255) gTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGAACGCGCAGGCGGGTGGAGCACCC‑Ct‑t > 2:918822/1‑239 (MQ=255) gTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTAGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGcacccttct > 1:635092/1‑234 (MQ=255) ggCCCCCCTCACAAAACCTTGGCCCACATTTACGCGATGAGGGAAGGCCCGGAACCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTAGCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTgcgg < 2:936171/239‑1 (MQ=255) ggCCCCCATCACCAAACCTGGGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATTCAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTgcgg < 1:186509/239‑1 (MQ=255) ggCCCCCATCACAAACCCTGGGCCACCATTTACGCGATGAGGCAAGGCCAGGATCAAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTgcgg < 2:271193/239‑1 (MQ=255) ggCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTgcgg < 1:622476/239‑1 (MQ=255) gCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCCCTATTGCCCTCCCGGCGCACGCGCAGGC‑GGGTGGGCACCA‑CT‑TTccggg > 2:1057770/1‑238 (MQ=255) gCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGGCCCC‑CT‑TTccggc > 1:347379/1‑239 (MQ=255) gCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGAACGCGCAGGC‑GGTTGAGCCCCA‑CC‑TTgcggc > 1:575904/1‑239 (MQ=255) gCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGAAAGCCCGCCACCATTGCCCTCCCGGCGCCCGCGCAGCC‑GGTTGGGGACCC‑CA‑TTtcggg > 2:789712/1‑238 (MQ=255) cACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCGGGCTACGAAAGCTGCACACCATTGCCCCCCCGGCGCACGCGCAGGG‑GGGTGGGCACCA‑Cc‑ttcggggg > 2:1085106/1‑233 (MQ=255) cACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATCTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTAACCAGCGTCCCAGGCTACGATAGCTCCCCACTATTGCCCTCCCCGGCCACGCCCAGGC‑GGTTTGGCACAC‑CT‑TTgcgccg > 1:535413/1‑239 (MQ=255) ctcccaAAACCTTGGCCCACATTTACGCGAGGAGGCAAGGCCAGGACCCAATACAACAAGATGAGGAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTa < 2:796940/235‑1 (MQ=255) aTCACAAAACCTGTGCCCACATTTACGCGATGGGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACCCCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTa < 1:741376/239‑1 (MQ=255) aTCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCC‑CT‑TTGCGGCGGC‑GTa > 1:738177/1‑239 (MQ=255) aTCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGACCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGTGAACCG‑CA‑TTTCTGCGCC‑Gt > 1:981425/1‑238 (MQ=255) aTCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCACCGGCCCTGCCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCCCAGGC‑GGGTGAGGCCAA‑CC‑TTgcgggggggcg > 2:174167/1‑235 (MQ=255) aTCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCAAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCCCTATTGCCCTCCCGGCGCAAGCGCAGGC‑GGTTGGCCACCC‑CTCTTgcgccggctc > 1:443175/1‑237 (MQ=255) aTCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAAAGCCAGATTCCCCAGCGTCCCTGGCTACGAAAACTCGCCACTATATCCCCCCCGGCCCACCCGCAGGC‑GGTGGCGCACCC‑CT‑TTggcgaggcccc > 2:345186/1‑229 (MQ=255) ccaaaacCCGGTGCCCACATTTACGCGATGGGTGAAGGCCAGGATCCAATACAACAAGAAGAGTAACCCCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGc < 2:716513/236‑1 (MQ=255) cacaAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTTGGCACCA‑CT‑TTGCGGCGGC‑GGAg > 1:49024/1‑238 (MQ=255) cacaAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCACCTATTGCCCCCCCGGCGCACGCGCAGGC‑GGTTTGGGACCC‑CC‑TTgagggggc‑gggg > 2:775653/1‑235 (MQ=255) aaCCTGGGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCttt < 1:558842/239‑1 (MQ=255) gtgCCCCCATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGga < 1:775653/239‑1 (MQ=255) gtgCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCCCTATTGCCCTCCCGGCGCCCGCGCAGGC‑GGTTTGGCACCACCT‑TTCCGGGGGG‑GTGCCCCttttggg > 2:311892/1‑235 (MQ=255) gtgCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGGGCACCA‑CC‑TTGCGGCGGC‑GGAGCCCCTTGTggg > 1:637354/1‑238 (MQ=255) gtgCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGATATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGACAGCCAGATTACCCAGCGTCCCGGGCTACGATAGCTCGCCACTATTGCCGTCCCGGCGCACGCGCAGGC‑GGCTGCGCCCCC‑CA‑TTTCGGCGGC‑GTATCCCGTTGTGga > 1:702727/1‑239 (MQ=255) tgCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAATAGAGCCAGATTACCCAGCGTCCCTGGCTCCGATAGCTCGCCACTATTCCCCTCCCGGCGCCCGCCCACGCGGGTTGAGCACCC‑AT‑CTCGCGCGGC‑GTACCCCttttggga > 2:290878/1‑234 (MQ=255) gcccccactttACCCGATGGGGAAAGGCCAGGATCCAATTAAACAAGAAGAAGAAACCCCGATTTGGCCGAAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGga < 2:77372/230‑1 (MQ=255) gCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGgaga < 1:277013/239‑1 (MQ=255) cccACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTTAGCACCC‑CC‑TTTCGGCGGC‑GGAACCCTTTGTgggggg > 1:271504/1‑235 (MQ=255) tACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTTAGCGACA‑CT‑TTTCGGCGGG‑CTAGCCCCTTTTGGAGAGCG‑acttg > 1:77372/1‑234 (MQ=255) cgcgATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAACGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCCCGCCCAGGC‑GGGTTAGCCCCA‑CC‑TTGGGGGGGCGGCGGCCCTTTGGGGAGGGCggtagcac > 2:697753/1‑232 (MQ=255) cgcaATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACCCCCAGTTTGGCCGGAAAACAATCAGGTTAACCGGCCAGGGCATGATTGGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑GTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTagcag < 1:311892/239‑1 (MQ=255) gcgATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTgcgg < 1:300952/206‑1 (MQ=255) cagagCCAAGGTCCAATCAAACAAGATTAGTAACCCCCTGTTTGGCCGGAAAACAATCAGGGTATCCGGGCAGGTCATGGTTTGCAGCAACAGGGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCtgtg < 2:174181/234‑1 (MQ=255) aTCCAATACAACAAGATGAGTAACCCCCAATTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCgcag < 1:689228/237‑1 (MQ=255) aaTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGGCCCC‑CC‑TTGCGGCGGC‑GGAGCCCCTTTTGGGGAGCG‑ATTTGCAGCAGGC‑GTGTGCCGCGCCGCAGgc > 1:639737/1‑238 (MQ=255) aaTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGAC‑GGTTGGGCACCC‑CT‑TTGCGGCGGC‑GTTGCCCCTTTGGGAG‑GCGCATTAGCAGAAGGCCTTGT‑GGGCGCAGCAGgc > 1:547237/1‑238 (MQ=255) aTACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCAACA‑CT‑TTGCGGCGGC‑GTAGCCCCTTGTGGGGAGCG‑ATTAGCAGCAGGCTGGGT‑CGGGCCCGCAGgcgc > 1:399774/1‑239 (MQ=255) caaGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCCGGC‑GGTTTGACACCC‑CT‑TTTCGGCGGC‑GTAGCCCCCTTTGGGGGGCG‑AGTAGAAGCCGGATGGGT‑CGGCGCACCAgggggccgagg > 2:822138/1‑230 (MQ=255) gATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTTCGGCGGC‑GTAGCCCTTTTTGGAGAACG‑AATAACAGCAGGCTGTGT‑CGGCGCCGCAGGCGCAGTAACttc > 1:101407/1‑237 (MQ=255) gATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCAACA‑CT‑TTTCGGCGGC‑GTAGCCCCTTTGGGGGGGCG‑ATTTACAGCAGGCCGTGT‑CGGCGCAGCCGGCGCAGGAGCTGc > 1:804272/1‑239 (MQ=255) tGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTTCCGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGACGCAAGCTGGGT‑CGGGCCAGCAGgggccatgacttgca > 1:721336/1‑228 (MQ=255) ttGGCCCGAAAACAAACAGGGTATCCGGCCCGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGGCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGt < 1:957207/238‑1 (MQ=255) gCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTTAGCACCA‑CT‑TTTCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGGGCCGCAGGGGCAGTAGCGGCATTTCCTGtgccccggtgtt > 1:672497/1‑228 (MQ=255) ccGGAAAACAAGCAGGTTACCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGGGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTtgct < 1:789712/238‑1 (MQ=255) aaaCAAGTAGGTTATCCGGCCAGGGCATGATTGTCCGCAACAGCGCAATGAGGAAAGAGACGCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGa < 2:399774/238‑1 (MQ=255) aaaactccggTTATCCGGCCAGGGAATGAGTTGCAGACACAGCGCAAGGAGGAAAGAGTGGCAGATTACCCAGCGTCCCTGGCTACGATAGCTAGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGt < 2:148199/231‑1 (MQ=255) aaTCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCgg < 1:918822/239‑1 (MQ=255) tCAGGGTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCt < 1:822138/239‑1 (MQ=255) cgggTTAGCCGGCCGGGGCATGATTTGCGGCAACCGCCGAAGGGGGAAAGAGGGCCAGATTACCCAGGCTCCCTGGCTAGGATAGCTCGCCGCTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCgg < 2:347379/234‑1 (MQ=255) tttCCGGCCAGGGCATGATTTGAAGCAACCGCGCAATGAGGAAAGGGAGCCAGGTTACCCAGCGTCCCGGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGa < 2:672497/237‑1 (MQ=255) tATCCGGGCAGGGGATGATTGGCAGCAACAGCGCAATGAGGAAGGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCg < 1:302437/238‑1 (MQ=255) gcagcaACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCCCCA‑CT‑TTgcggcggc‑g > 2:111437/1‑119 (MQ=255) gcagcaACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTgcggcggc‑g < 1:111437/119‑1 (MQ=255) gcAATGAGGAAAGGGAGCCAGATTTCCCAGCGTCCCTGGCTACG‑TAGCTCGCCACTATTGCCCGCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCa < 1:925498/239‑1 (MQ=255) tGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGGGAGCG‑ATTAGCAGCAGGC‑GCGTGCGGCGCAGCAGGCGCAGTGGCTGCACTTCCTGTGGCCCGTTGTTGGGGGGTCGGCGGGGGGGTCCCCCTTTCCGGGTTCGGGGAATTTTCGccccccg > 2:1079250/1‑236 (MQ=255) agagCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCg < 1:51478/239‑1 (MQ=255) gagCCAGATTCCCCAGGGGCCCGGGCTTCGATAGCGCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑TTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGc < 1:1079250/237‑1 (MQ=255) ccccccTGGCTACCATAGCCCGCCACTTTTGCCCGCCCGGCGCACGCGCAGGG‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGc < 2:1068255/236‑1 (MQ=255) gTCCCTGGCTACGATAGCTCGCCCCTATTGCCCGCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGc < 1:953495/238‑1 (MQ=255) gTCCCTGGCTACGATAGCTCGCCACTTTTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGc < 1:616493/238‑1 (MQ=255) gTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGGGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGca < 1:788852/239‑1 (MQ=255) gTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GGAGCCCCTTGTGGAGAGCG‑GTTTGCAGCAGGCTGTGT‑CGGCGCCGCAGGCGCAGTAGCTGCCATTCCCGTCGCCGGTGTCTGCGGGGGGGGGGGAGGGATCCCCCGTGCC‑GGGTTCGGTAACTTTTGCCCCCCCGCGTCTGGGGCAGGCAAAAccccgggg > 2:111741/1‑232 (MQ=255) tGGCCACGATAGCCCGCCCCTTTTGCCCTCCCGGCGCACGCGCAGGC‑GGTGGAGCCCCA‑CT‑TTGGGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCatcatc < 2:639737/239‑1 (MQ=255) gagaGCTCGCCACGATGGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATc < 1:368513/235‑1 (MQ=255) aGCCCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGGGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGa < 1:290878/238‑1 (MQ=255) tATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCgca < 1:665730/96‑1 (MQ=255) tATGGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGcatc < 1:749484/216‑1 (MQ=255) gcgcccgccGGGGCTCGCGTGGGG‑GGTTGGGCCGCC‑CT‑TTGCGGCGGT‑GTAGCCGTGTGTGAGGAGGG‑ATTGGGTGGAGGGTGGCT‑CGGCGCAGCAGGCGCGGGAGCTGCAGTCGCTGGCGGCGGTTGGTGGGGAGGCGGGTGAGCGATCCGCGGTGCC‑GGGTCAGGTAACTTGGGCCCCAGCGGGTCTGGCGAAGGCAATACCTGCGGCATCATCTGGATCGGACGTTCATTAGCaa < 2:535413/231‑1 (MQ=255) gCCCTCCGGGCACACGCGCAGGC‑GGTTGAGCACCC‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAaca < 1:323311/239‑1 (MQ=255) cccGGCGCACGGGCAGGC‑GTGTGGGGCCCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATg < 1:345186/239‑1 (MQ=255) ccggcgtggTTGGGCACCG‑CT‑GTGGGGGGGC‑GTAGCTCTGTGTGGAGAGGT‑ATTAGGAGCCGGCTGTGT‑CGGCGCAGCAGGCGCCGTATCTGCATTTCCTGTCGCCGGTTGCTTCGGCGTCGGCTGGGCGTTCCCCTTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATGCCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCg < 2:536193/232‑1 (MQ=255) ggTTGGGCCCCC‑CT‑TGGCGGGGGG‑GTAGCCCTTTGGGGAGGGCG‑ATTAGCAGCAGGCTGGGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAt < 2:738177/239‑1 (MQ=255) ggTTGAGCCCCA‑CT‑TGGCGGCGGG‑GTAGCCCTTTGTGGGGAGCG‑ATTAGCAGCAGGCTGTGG‑CGGCGCATCAGGCGCAGTAGCTGCATTTCCTGTCGGCGGTTTCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAt < 2:290236/239‑1 (MQ=255) | TCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCAATACAACAAGATGAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGC‑GGTTGAGCACCA‑CT‑TTGCGGCGGC‑GTAGCCCTTTGTGGAGAGCG‑ATTAGCAGCAGGCTGTGT‑CGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTTGCC‑GGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCAT > NC_000913/3398676‑3399141 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |