Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,580	T→A	18.8%	T63S (ACC→TCC)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,580	0	T	A	18.8%	176.5 / 11.1	92	T63S (ACC→TCC)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (59/11);  new base A (2/16);  total (63/29)
Fisher's exact test for biased strand distribution p-value = 1.01e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGT  >  NC_000913/4065344‑4065817                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 gATCAACGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTa                                                                                                                                                                                                                                                 <  1:856823/238‑1 (MQ=255)          aCCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGAAA‑AACGGGTAAAAAACT‑Tc                                                                                                                                                                                                                                       >  2:387569/1‑238 (MQ=255)           ccATACGAGCAGGTCATCAGGCTGAATGAAAGGCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGc                                                                                                                                                                                                                                     <  1:359484/239‑1 (MQ=255)            cATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑GGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACCCGGTAAAAAACT‑TCGc                                                                                                                                                                                                                                     >  2:332355/1‑238 (MQ=255)              tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑GGTAAAAAT‑CCGGTGAG‑CATATCGGAAA‑ACGCGGTAAAAAACT‑TCGccac                                                                                                                                                                                                                                  >  1:901148/1‑239 (MQ=255)              tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGGAAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGccac                                                                                                                                                                                                                                  >  1:1009450/1‑239 (MQ=255)              tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCCCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAATCG‑GGTAAAAAT‑CCGGTAAG‑CATTTCGGTAA‑ACGCGGTAAAAAACT‑TCCccac                                                                                                                                                                                                                                  >  2:296645/1‑239 (MQ=255)                      gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATCTTTT‑TCCGCGAGACG‑AGTAAAAAT‑CGGGTGAG‑CAAATCGGTAA‑ACGCGGCAAAAAACT‑TCGCCACC‑CG‑AAAGa                                                                                                                                                                                                                        >  1:40210/1‑239 (MQ=255)                      gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGGGTCG‑GGTAAAAAT‑CCGGTGAG‑CAAATCGGAAA‑ACGGGGAAAAAAACT‑TCCCCACC‑Aa‑aaaaa                                                                                                                                                                                                                        >  2:1045845/1‑237 (MQ=255)                      gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGGG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCCCC‑AG‑AAAGa                                                                                                                                                                                                                        >  1:697068/1‑239 (MQ=255)                            caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGA‑GCCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGGCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGGTAATc                                                                                                                                                                                                                   >  1:68681/1‑238 (MQ=255)                               aCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGAGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATACAGGGCCGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCAGGTAG‑AGTAAAAAT‑CCGGTGAG‑CCTATCGGTAA‑ACGCGGTAAAAAAAT‑TCGCCAAC‑AA‑AAAAAAAAACCCGc                                                                                                                                                                                                               >  2:876112/1‑239 (MQ=255)                                 tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGGGTCG‑AGTAAAAAT‑CCGGTGGG‑CATATCGGTAA‑ACGCGTAAAAAAACT‑TCGCCACC‑AA‑AAAGAAAATCCGGCCa                                                                                                                                                                                                             >  2:316137/1‑239 (MQ=255)                                 tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCCCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGTTAA‑ACGCGGTAAAAAACT‑TCGCCCCC‑AG‑AACGCAAATccgccca                                                                                                                                                                                                             >  1:512234/1‑234 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAGGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATa                                                                                                                                                                                                           >  1:131818/1‑239 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCTCGATGCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AT‑AAAGATAATCCCGCCATa                                                                                                                                                                                                           >  1:567319/1‑239 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATa                                                                                                                                                                                                           >  1:733376/1‑239 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAAAT‑TCGCCACC‑AG‑AAAGAAAAACCCGCCaaa                                                                                                                                                                                                           >  2:1083069/1‑237 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CAGATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑GG‑AAAGTAAATCCCGCCATa                                                                                                                                                                                                           >  1:499058/1‑239 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGGGGG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGAAAATcccccaaaa                                                                                                                                                                                                           >  2:608746/1‑233 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAA‑CCGGGAAG‑CATAACGGTAA‑ACCCGGTAAAAAAAT‑TCGCCAAC‑AA‑AAAAAAAAACCCGCCaaa                                                                                                                                                                                                           >  2:486743/1‑237 (MQ=255)                                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCAAGTCG‑AGTAAAAAT‑CCGGTAAG‑CATATCGGTAA‑ACTCGGTAAAAAACT‑TACCCACC‑CG‑AAAGAAAATCCCGCCATa                                                                                                                                                                                                           >  1:1016194/1‑239 (MQ=255)                                         aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGcc                                                                                                                                                                                                                                    >  1:580009/1‑210 (MQ=255)                                         aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAAATGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGGAAAAAACT‑TCGCCACA‑GA‑AAAGATAATCCCGCCATAgaacga                                                                                                                                                                                                     >  1:119575/1‑234 (MQ=255)                                         aGTCCAAAAATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGcc                                                                                                                                                                                                                                    <  2:580009/210‑1 (MQ=255)                                           tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑CG‑AAAGAAAATCCCGCCATAGTAAGCAg                                                                                                                                                                                                   >  1:225100/1‑239 (MQ=255)                                           tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCa                                                                                                                                                                                                    >  1:485383/1‑238 (MQ=255)                                             cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATATTAAGCAGGc                                                                                                                                                                                                 >  1:1013372/1‑239 (MQ=255)                                             cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACACGGTAAAAAACT‑TCGCCACC‑AA‑AAAGAAAAACCCGCCAAAGaaaaaagga                                                                                                                                                                                                 >  2:640419/1‑233 (MQ=255)                                             cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATACCGGTAA‑ACGCGGTACAAAACT‑TCACCACC‑AG‑AAAGATAAGCCCGCCATAGTAAACAGGc                                                                                                                                                                                                 >  1:232166/1‑239 (MQ=255)                                             cAAACATCAAAAACCGCGGGGTGCCAGTCGTCTTTTTACCCGAAAAAAAAAG‑GGGGGT‑GGCAATAAACGGGA‑GTGT‑TGCAATTACCGCCCCCGAACCCGCTG‑CATAAAAAAAAAGGGGAAAAAATTTGC‑TTTTAGGCCAAATTTTTT‑CCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑CCGCGGAAAAAAACT‑TCGCCCCC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                 <  2:1009450/239‑1 (MQ=255)                                                    catAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAAATAATCCCGCCATAGTAAGCAGGCATCCCTa                                                                                                                                                                                          >  1:811516/1‑239 (MQ=255)                                                     atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAAAT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAACAAGGCATCCCTaa                                                                                                                                                                                         >  1:144992/1‑239 (MQ=255)                                                            gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTcaaaaa                                                                                                                                                                                                                                            >  1:201616/1‑184 (MQ=255)                                                            gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                  >  1:278920/1‑239 (MQ=255)                                                            gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                  >  1:127562/1‑239 (MQ=255)                                                            gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCACCCCTAATTCaaca                                                                                                                                                                                  >  2:184025/1‑236 (MQ=255)                                                            gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGGGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAAACCCGCCAAAGAAAACAGACAACCCTAAtaacacg                                                                                                                                                                                  >  2:303947/1‑233 (MQ=255)                                                                       aTCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAttt                                                                                                                                                                       >  1:130719/1‑239 (MQ=255)                                                                         tcgttttttAAACCGCAAAAAAAAGGGTGGG‑GGTAATAAAGGGGG‑GCGT‑TCAAATTAGCCCCGCGGGACCCTCTT‑AAAAAAAAAAAAAGGGAAGAAATTTGC‑CTTGAGGCCAAATTTTTT‑CCCGCGAGTGA‑AGAAAAAAT‑CCGGGGAG‑CATATCGGAAA‑ACGCGGAAAAAAACT‑CCGCCACC‑AA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                      <  2:701723/234‑1 (MQ=255)                                                                           tttttttAACCCGAAAAAAAAA‑GGGGGTGGGAAAAAAACGGGG‑GCTT‑TGAAATTACCCCGGCGGGAACGGTTG‑AATAAAAAAAAAAGGAACAAAATTTGC‑TTTTAGGCCCAATATTTT‑TCCGGGAGTAG‑AGAAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGAAAAAACCT‑TCGCCACA‑AG‑AAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                    <  2:270596/236‑1 (MQ=255)                                                                            cgtTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGAATCCCTAATTCATCGGTATAAAATTTgagga                                                                                                                                                                  >  1:441236/1‑239 (MQ=255)                                                                              ttttTACCCGCAAAAAAAAGGGGGGT‑GGCAAAAAATTGG‑‑GCTTGTGAAATCACCCCCGCCGCACCCGCAA‑TAAAAAAAAAAAAGGGAAAAAATTTGC‑CTTTGGGCCAATTTTTTT‑CCCGCAAGTGG‑AGAAAAAAC‑CCGTTGAG‑CATAGCGGAAA‑ACGCGGTAAAAAACT‑CCGCCCCA‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgaggag                                                                                                                                                                 <  2:733376/239‑1 (MQ=255)                                                                                 tAACCAGAAAAAAAAA‑GGGGGG‑GGGAAAAAAAGAGA‑GGGT‑TGAAAATGACCGCGCCGGAACCCGTGTTATAAAAAAAAAGGGGACAAAATTCGG‑TTTTGGCACCAGTATTTT‑TCGGAGATCCG‑AAAAAAACT‑CCGG‑GAGTCATATGGGAAA‑ACGGGGAAAAAACCT‑CCCCCACA‑AA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa                                                                                                                                                              <  2:1016194/239‑1 (MQ=255)                                                                                   cccgaaaaacaaaG‑GGTGTG‑GGAAATAAA‑TGGAGGCGT‑TGCATTCACCGCCGCGGAACCCGCTC‑AAAAAAAAAAAAAGGAACAAAATTTGT‑CTTTGGGCCCAATTTTTT‑GCAGGGAGACG‑AGTAAAAAT‑CGGGTAAG‑CATATCGGTAA‑ACGCGTAAAAAAACT‑TCGCCACA‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                           <  2:398146/233‑1 (MQ=255)                                                                                             aaaa‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCACGCCATAGTAAGCAGGGATCCCTAATTCACCGGGATAAAATTTGAGGAGATACAGAGTACCGagg                                                                                                                                                 >  1:504776/1‑237 (MQ=255)                                                                                                      gt‑ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCAt                                                                                                                                         >  1:772754/1‑239 (MQ=255)                                                                                                      gt‑ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAGGTACCGATGCAAAGcct                                                                                                                                         >  1:389985/1‑237 (MQ=255)                                                                                                         ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGGAGTTACAGAGTACCGATGCAGAGCATTa                                                                                                                                       >  1:338563/1‑239 (MQ=255)                                                                                                         ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCAGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGCGGAGATACAGAGAACCGCTGCAGAGCATTa                                                                                                                                       >  1:187535/1‑239 (MQ=255)                                                                                                                    ggA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGTAACAGGGAACCGAGGCAGAGCATTAAATTCGAACCg                                                                                                                            >  1:337540/1‑239 (MQ=255)                                                                                                                    ggA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCAGCCATAGTAAGCAGGCACCCCTAATTCATCGGTATAAAATTTGGGGGGAAAAAAATAACCGATCCAGAGCATTAAATTCAAACCg                                                                                                                            >  2:648874/1‑239 (MQ=255)                                                                                                                         gtt‑tGCACTTACCGCCGTCGCACCCTCT‑‑TATAAAAAAAAAAGGGGAAAAATTTGCGCTTGAGG‑CCAATAATTT‑TCCCCGAAGCGAAAAAAAAAG‑CCGGCGAC‑TATATAGATAA‑CCGCGATAAAACTCT‑TCGCAACC‑AG‑AAAGAGAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACg                                                                                                                         <  2:68681/236‑1 (MQ=255)                                                                                                                           t‑tGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTc                                                                                                                       >  1:436422/1‑238 (MQ=255)                                                                                                                              gCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGAAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAAGCATTAAATTCAAACCGACGTccc                                                                                                                     >  2:114075/1‑236 (MQ=255)                                                                                                                                 aTTAACGCCGCCGGAACCGCTG‑CATAAAAAAAAAAGGGACGAAATTTGC‑TTTTAGGCCCAATTTTTT‑CCCGCGAGGCG‑AATAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑CCGCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCccat                                                                                                                 <  1:387569/239‑1 (MQ=255)                                                                                                                                  ttAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGGCGCccat                                                                                                                 >  1:1001162/1‑238 (MQ=255)                                                                                                                                         cgccgGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTTAGGAGATACAGAGTACCGATGCAGAGCATTa                                                                                                                                       >  1:748694/1‑209 (MQ=255)                                                                                                                                          gccgGAACCGCTG‑CATATAAAATAAAGGGACGAATTTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGGAGATAAAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGc                                                                                                        >  1:943050/1‑239 (MQ=255)                                                                                                                                                       caataaAAAAAAAAGGGAAGAAATTTGC‑TTTTAGGCCAATTATTTT‑CCCGCGAGTCG‑AGTAAAAAC‑CCGGTGAG‑CATATCGGTAA‑ACGCGGAAAAAAACT‑TCGCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCg                                                                                            <  1:1045845/237‑1 (MQ=255)                                                                                                                                                             aaaaaaaaGGGAAAAAATTTGC‑CTTAAGGCCAAATTTTTT‑CCCGGGAGCAG‑AGAAAAAAT‑CCGGTGAG‑CATAGCGGTAA‑ACGCGGAAAAAAACT‑TCGCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                      <  1:114075/239‑1 (MQ=255)                                                                                                                                                              aaaaaaaaGGACCAAATTGTC‑CTTTGAGTCCAATTTTTTGCCCGAGAGAAG‑ATAAAAAAT‑CCGGTAAG‑CA‑AGCGGAAACACGCGGAAAAAAACT‑TCGCC‑CC‑AGAAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                      <  2:504776/239‑1 (MQ=255)                                                                                                                                                                ataaaGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAg                                                                                   >  1:638569/1‑239 (MQ=255)                                                                                                                                                                        aaaaaaTTTGC‑CTTGAGCCCAAATATTCT‑GCCGCGAGTAG‑AAAAAAACT‑CCGGTGAG‑CATTCGGGTAA‑CCGCGTTAAAAAACT‑TCGCCCCA‑AA‑AAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAt                                                                            <  1:640419/235‑1 (MQ=255)                                                                                                                                                                         cGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAg                                                                                                                                                                                                      >  1:901088/1‑115 (MQ=255)                                                                                                                                                                         cGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCAAACCAAAGGCGATTTTTTCCCCCAGATTCAGTTTTAAtta                                                                          >  2:680391/1‑237 (MQ=255)                                                                                                                                                                                tGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGCTTTTTTCCCGCAAATTCAGTTTTAATTTCAGTGGa                                                                   >  2:567156/1‑239 (MQ=255)                                                                                                                                                                                       tAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGCCGCCCATACCATAGGCGATTTTTTCCCCAAAATTCAGTTTTAATGTAAGTGGATTATgg                                                             >  2:514077/1‑239 (MQ=255)                                                                                                                                                                                        agcccaaaTTTTTT‑CCCGCGACTCG‑AGAAAAAAG‑CCGGTAAG‑TAT‑TGGGGAA‑AATGGGAAAAAAACTCCCCCCACA‑AA‑AAAAATAACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                            <  2:144992/233‑1 (MQ=255)                                                                                                                                                                                        agccacAATATTTT‑TCCGCGAGTCA‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑CCGCGGTAAAAAACT‑TCGCCACA‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                            <  1:569923/234‑1 (MQ=255)                                                                                                                                                                                              aaTATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTTTGTTCAGACa                                                      >  1:77030/1‑239 (MQ=255)                                                                                                                                                                                              aaTATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACa                                                      >  1:558506/1‑239 (MQ=255)                                                                                                                                                                                                  tttt‑cCCGGGAGACG‑AGAAAAAAC‑CCGGTGAG‑CATCTAGGAAA‑CCGCGG‑AAAAAACTCTCCCCACC‑AG‑AAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAAt                                                  <  1:996985/239‑1 (MQ=255)                                                                                                                                                                                                     ttcccgaaattcg‑agaAAAAAC‑CCGGTGAG‑CATCTCGGTAA‑CCGCGATAAAACACT‑CCGCCCCC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                <  1:486743/229‑1 (MQ=255)                                                                                                                                                                                                     ttcccGCGAGTCT‑AAAAAAAAC‑CCGATGAA‑CATATGGGAAA‑CAGGGGAAAAAACCT‑TCCACACC‑AA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                <  1:567156/236‑1 (MQ=255)                                                                                                                                                                                                     gtcgcgcgAGTAG‑AGTAAAAAT‑CCGGGTAG‑AATATCGGTAA‑ACGAGATAAAAACCT‑TCGCCACC‑AG‑AAAGAAAATGCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACTTAATCa                                                <  1:568381/235‑1 (MQ=255)                                                                                                                                                                                                       tCCGGGAGT‑G‑AATAAAAATCCCGGTGAG‑AAATTGGGAAA‑ACGCGGTAAAAACCT‑GCGCCCCA‑AA‑AAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                              <  1:1083069/239‑1 (MQ=255)                                                                                                                                                                                                         cgcgAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCAGATTCAGTTTTAATGTCATTGGATTATGGTCAGACaaaacaacc                                              >  2:285819/1‑232 (MQ=255)                                                                                                                                                                                                           cgagtcg‑agtAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGCGCATTAAATTCGAACCAACGTCGCCCATATCATAGCCGATTTTTTCCCGCAGATTCAGTTTTAATACCATTGGATTATGGTCAGACTTAATCACTCCtt                                          >  1:1055582/1‑239 (MQ=255)                                                                                                                                                                                                           cgagtcg‑agtAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGGGATTTTTTCCCGCAGATTCAGTTTTAATGTACATGGATTATGGTTAGAACAAAACACTCCtt                                          >  2:729659/1‑239 (MQ=255)                                                                                                                                                                                                           cgagtcg‑agtAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGGCAGAACTAATAACTCCtt                                          >  1:668856/1‑239 (MQ=255)                                                                                                                                                                                                                             cgggggAG‑CTTATGGGAAA‑ACGCGGAAAAAACCT‑CCGCCCCA‑AA‑AAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAggg                          <  2:127562/234‑1 (MQ=255)                                                                                                                                                                                                                              gggTGAG‑AATAGGGGAAA‑ACG‑GGGAAAAAAAT‑TCCCCACCAAA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGc                         <  1:285819/238‑1 (MQ=255)                                                                                                                                                                                                                                ggagg‑CATAGCGTAAA‑ACGGGGAAAAAAACT‑TCGCCCAA‑AG‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                        <  1:729659/234‑1 (MQ=255)                                                                                                                                                                                                                                    g‑CATATCGGAAA‑ACGTGAAAAAAACTT‑TCCCCACC‑AG‑AAATATATCCCCGCCATAGTAAGCAGGCATCCCTAATTCAGCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcgtcgt                    <  1:648874/238‑1 (MQ=255)                                                                                                                                                                                                                                      cATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGAGGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGGGGATTATGGTCAGCCATAATAACCCTTTATAACGCCCCCAAGGGGGTGGttt                  >  2:95399/1‑239 (MQ=255)                                                                                                                                                                                                                                        tttgggaca‑aCGCGGAAAAAAATC‑TCCCCAAA‑AA‑AAAAATAACCCCGGCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                <  2:1023656/231‑1 (MQ=255)                                                                                                                                                                                                                                        tttatgaaa‑aCGGGGAAAAAACCC‑TCCCCAAC‑AG‑AAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                <  2:697068/232‑1 (MQ=255)                                                                                                                                                                                                                                        ttgcGGTAA‑ACGCGAAAAAAACCT‑ACACAACA‑AA‑AGATACACTCCCGCCATAGTAAGCAGGCATCCCTAATTCAACGGTATAAAATTGCAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCTAACCGACGTCGCCCATACCATAGGCGATTTTTGCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                <  1:54610/236‑1 (MQ=255)                                                                                                                                                                                                                                                a‑aCGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTTTAACGCCCCCCAGGGCGCCGTttattattttt         >  1:9150/1‑238 (MQ=255)                                                                                                                                                                                                                                                a‑aCGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttattattatt         >  1:756622/1‑238 (MQ=255)                                                                                                                                                                                                                                                     ggatAAAAAACT‑TCCCCACA‑AG‑AAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACg      <  1:595647/234‑1 (MQ=255)                                                                                                                                                                                                                                                       gTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGCCGTttattattattaacccgg  >  2:234842/1‑233 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 GATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATCGGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGT  >  NC_000913/4065344‑4065817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑