| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,444,689 | T→G | 14.3% | V193G (GTG→GGG) | tamB → | translocation and assembly module for autotransporter export, inner membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,444,689 | 0 | T | G | 14.3% | 252.0 / 2.6 | 119 | V193G (GTG→GGG) | tamB | translocation and assembly module for autotransporter export, inner membrane subunit |
| Reads supporting (aligned to +/- strand): ref base T (40/54); new base G (15/8); total (57/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
GTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTGGACGCCC > NC_000913/4444457‑4444925 | gTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGCCACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGCCCTGCTGATTTCCCTGCCGAAAGCGGCGGAAATGGGCCGGGAAGAGGTGGGCg > 1:162159/1‑238 (MQ=255)gTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGATCTGCCGAAAATGGCGGCAGAGGGGCCGGAAGAAGGTGTCgg > 1:390424/1‑238 (MQ=255)gTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCGGTTTGCTCTGCCGAACGAGGGGGGAGTGGCGACGGAAGAAgggggcca > 2:1018497/1‑235 (MQ=255) agGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGGGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCCGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGCCGGAAGAAAGGGGGGAACCGGAAAtt > 1:391270/1‑239 (MQ=255) agGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGCAAGTGGCGGAAGGGGCGGAGGGAGAAAGGGTCGAACCGTAAAtt > 1:424869/1‑239 (MQ=255) agGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGGGGCGGAAGTGGCGCAGGAAGAAGGGGTCGAACCGAAAAtt > 1:599206/1‑239 (MQ=255) cGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGCCGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGAGGCGGAAGAGGCGCTGGAAAAAGTGGCTGAACCGAAAAATGAAAAtaaac > 1:814867/1‑235 (MQ=255) cGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGTAAGGCCTGATGATTGATCTGCCGAAAGAGGCGGCAGGGGGGCGGGGAGAAATGGTCGACACGACAATTTaaaaacagc > 1:157473/1‑234 (MQ=255) gTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCACCATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCGGGCCTGAACTGGCAGGAGAAAACCCTGACCCCGAAACAGACCGCGCTGACAGGGCTGCTGATTGCGCTGCCGAAAGTGGCGGAAGGGGCCCGGGGAAAAGGGGTCGAACCGAAAAATTaaaaaacgaa > 2:166025/1‑232 (MQ=255) gTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGCAGAAGGGGTCGAACCGAAAATTGAAAAACCGcc > 1:1058343/1‑237 (MQ=255) cGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGCGGCGCAGGAAGAATGGGTCGGACCGAAAATTGAAAAACCGAAGCCg > 1:351514/1‑239 (MQ=255) cGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGAGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGGATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGCCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGGGGCGAGAGGGCGGCAGGAAGAAAGGGCGGAAACGCAAAATGaaaaaaccgaacca > 2:238793/1‑229 (MQ=255) aCGCCGTATCCCATCCCCCGGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCt < 1:888282/239‑1 (MQ=255) cGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGTGAAAACCCTGACCCTGAAACCGACGTCTCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGACGAGGGGCAGGACGAAGTGGGCGCACAGAAAATTGTAAATACGCAGACGGAGGGAAagcagccc > 1:495005/1‑237 (MQ=255) cGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGAGGGGCAGGGAGAAAGGGTCGAACCGAAAATTGAAAAACCCCAGCCGGAGGAAAAACCGCCCGGc > 2:519499/1‑239 (MQ=255) atccactctcccTGAGCCGGTGGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGTGCTTCCCCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑Gaa < 2:187763/230‑1 (MQ=255) aTCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCGGATTGCTCTGCCGAAAGGGGGGGGAGTGGCGCAGGGAGAAGTGGGGGAACCGGAAATTGAAAATGCGCAGCCAGATGAAAAAGCGCTCCGG‑Gaa > 2:856621/1‑239 (MQ=255) aTCCCAACACCCGGACACGGGGGGCACGGGACAACGTTAAAAGTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑Ga < 1:478741/238‑1 (MQ=255) tCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAAGGGCGGAAGGGGCCCAGGGGAAAGGGGGCGAACCGAAAAATGAAAATCCGCAGGCGGGTGGAAAAGCGCGCGGC‑GAAAcaggg > 2:816768/1‑235 (MQ=255) cccccTGACACGGGGGGCCCTGGACAACGTCAACATTAAGATTGATGCCCCCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGa < 2:1054725/237‑1 (MQ=255) cACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGCAGGAAGAAGGGGTCGAACCGCAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCCCGGC‑AGAACGCCGa > 1:862608/1‑239 (MQ=255) cccTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATTAAAAACCGCTCCGC‑GCAAACCTGaaa > 1:662117/1‑239 (MQ=255) cccTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGaaa < 1:830349/239‑1 (MQ=255) gTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGGGGGCGAACCGCAAATTGAAAATCCGCGACCGGATGACAAGCCGCCGGGG‑GAAAAGCCTAAAGATCCCtttt > 1:36632/1‑238 (MQ=255) cACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGAGGGGCCAGGAGGAGGGGGCGGACCGAACATTGAAAATTCGCAGCCGGGTGAAAAACCGCCCGGC‑GGAACGCTGAAAAATATCTTTtctcc > 2:506579/1‑237 (MQ=255) aCTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGcc < 1:732189/239‑1 (MQ=255) gACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGCAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAATGGGCGAACCGAAAATTGAAAATCCGCAGACGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTTTTGCCCggg > 1:73401/1‑238 (MQ=255) cGTCAACATTAAGATTGATGCCACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGc < 1:519499/239‑1 (MQ=255) cGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCTCCCGGAATTg > 1:16154/1‑238 (MQ=255) cGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGGGGTCGAACCGAAAATTGAAAATCCGCAGCCGGAGGAAAAACCGGCCGGC‑GAAAAGCCGGAAAAACCCTTTTTTCGCCCGGGAtttg > 2:494821/1‑237 (MQ=255) aaCTTTAAGATTTATGACCCCCAGGTATCGGGGAGGGACTTCCCCCCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGa < 2:651545/239‑1 (MQ=255) aCATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGCAGGGAGAAGTGGTCGAACCGGAAAATGGAAATTCGCAGCCGGGTGGAAAGCCGCCCGGC‑GAAACGCCGAAAGAACTCTTTTTTCCCCCGGTaagccgggaa > 2:544335/1‑230 (MQ=255) aaGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGa < 1:547008/161‑1 (MQ=255) aaGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGa > 2:547008/1‑161 (MQ=255) aaGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATTAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTTTCGCCCGGGATTGCCGGAAAtaa > 1:234044/1‑236 (MQ=255) aGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAACGTGGCGGAAGTGGCGCAGGAAAAAGTGGTCGAACCGAAAATTGCAAATCCGCAGCCGGATGAAAAAGCGCCCACG‑GAAAGCCGTAAAGAACCCTCTTTTCCCCCCGGATTGCCGGGAAtaaac > 2:703727/1‑235 (MQ=255) gatgaCACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGCAAAGCCGCCCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTTCCGGAAATGACCGACGt > 1:24224/1‑239 (MQ=255) ccacGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGCAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCCCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGCATTGCCGGAAATGACCGACGGGCAttt > 1:554919/1‑238 (MQ=255) ccacGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCCCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTTTTGCCGGAAATAACCGAAGTGccatt > 1:165978/1‑234 (MQ=255) cacGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGAGGCCCACGAAGAAGAGGTCGAACCCGAAATTGAAAATCCCCAGCCGGATGAAAAGCCGCCCGGC‑GAAACACTGAAAGATTCCCTTTTTCCCCCGCTCATGCCGGTAAGAACCGACGGATATTTGc > 2:1057397/1‑239 (MQ=255) ggTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTTACCCTGAACCCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGc < 1:494821/239‑1 (MQ=255) ggTATCGGTGATGGACTTCACCGCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGc < 1:544335/239‑1 (MQ=255) ggTATCGGTGAGGGACTTCCCCGCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCAGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATTTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGc < 1:1013309/239‑1 (MQ=255) ggTAGCGGTGATGGACTTCACCTCCGGACTGAACTGGCAGGAGAAATCCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGc < 1:614897/239‑1 (MQ=255) tATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGGAAGATCTCTTTTCTCGCCCGGAATTGCCGGGAATGACCGACCGGCATTTGC‑CGCtt > 1:1061519/1‑239 (MQ=255) aTCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCtt < 1:663676/238‑1 (MQ=255) tgagtgatttCCCCCCCGGCCTGAACTGGCCGGAGAAAACCCCGACCCTGAAGCCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCt < 2:764937/230‑1 (MQ=255) tGATGGACTTCCCCCCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCt < 1:655697/238‑1 (MQ=255) aTGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGTAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAAAGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGaa > 1:545127/1‑239 (MQ=255) ggACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCCCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGGATTGCCGGAAATGACCGACGGGCATTTGC‑CTCTTAACCttgaaa > 1:978798/1‑234 (MQ=255) gACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGGAAATTGGAAATCCGGAGCCGGCGGAAAAGCCGCGAGGCGGAAACCGGGAAAAAT‑ACTTTTTTGCCCCGGGATTGCCGGGAAGAACCGACAGGCATTTGCGCGGTTAACGTGa > 2:241588/1‑236 (MQ=255) ttacccgcgggCCTGAACTGGCAGGAAAAAACCCTGGCCCGGAAACCGAAGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTg < 2:234044/229‑1 (MQ=255) tcccccccGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGaa < 1:131168/233‑1 (MQ=255) tCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGaa < 1:619659/239‑1 (MQ=255) tCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGGCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGaa < 1:418351/239‑1 (MQ=255) tCACCCCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGaa < 1:474177/239‑1 (MQ=255) ccccccccggccggAACTGGCGGGAGAAAACCCCGACCCTGAAACCGACGGCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGCAGGAAGAAGTGGTAGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGaa < 2:665027/233‑1 (MQ=255) ccTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCCCGGC‑GAAACGCTGTAAGATCTCTTTTCTCCCCCGGTATTGCCGGCAATGCCCGACGGGCATTTGC‑CGCTTAACCTGAACAttttagcg > 1:665027/1‑233 (MQ=255) gaaaaaCCCTGACCCTGAAACCGGCGTCCGTGAAAGGCCTGCTGATTGCGCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTgcg < 2:862608/236‑1 (MQ=255) ctgccccggaAACCGGCTGCGTTGAAAGGCCCGCTGATTGCGCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACggc < 2:495005/239‑2 (MQ=255) gaccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGGATTGCCGGAAATGACCGACGTGCATTTTC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGGCCGGGACGGGCg > 1:894240/1‑239 (MQ=255) gaccctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGGATTGCCGGAAATGACCGACCGGCATTTGC‑CCCTTAACCCTGACCTTGGAGAGTTTAAAGGGGGGCAGGCGCGCGGGAgggggg > 2:835218/1‑237 (MQ=255) ccccgaAACCCCCGTCGCTTAAAGGCCTTGTGATTGCTCTGCCGAAAGGGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGac < 2:391270/239‑1 (MQ=255) cctgaAACCGGCGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGaca < 1:899913/239‑1 (MQ=255) cctgaAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCGGCCGAAAGGGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGaca < 1:816768/239‑1 (MQ=255) aaCCGGCGGCGCTGAAAGGCCTGCTTATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGa < 1:245079/238‑1 (MQ=255) aCGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GCAACGCTGAAAGAACTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGGGCATTTGC‑CGCTTAACCCGAACATTGAAGGGTTTAAGGGGGAGAAACTGCGCGGGAGGGGGGAAACGGACacacc > 2:12728/1‑235 (MQ=255) aCGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAGAAGTTTAAGGGGGAGCAGCTGCGCATGACGGGCGACACGGCCATCa > 1:715574/1‑238 (MQ=255) gCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGCCATCACCGTGa > 1:626910/1‑239 (MQ=255) cTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACa < 1:741104/229‑1 (MQ=255) gtaaaTGCCTGGTGATTTCCCTGGCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGGGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGACGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAg < 1:615363/236‑1 (MQ=255) gAAAGGCCTGCTGATTGTCCTGCCGAAAGGGGCGGAAGGGGCACAGGAAGAAGTGGACGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTGTCGCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCa < 2:150662/239‑1 (MQ=255) gAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCa < 1:439768/239‑1 (MQ=255) tgttctgTTTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCAt < 2:16154/233‑1 (MQ=255) gctgctgAGTGTTCTGCCGAAAGTGGCGGAAGGGGCCAAGGAAGCAGGGGCCGAAACGAAAATTGAAAATCCGGAGCCGGATGAAAGGCCGCTCGGC‑GAACCGCGGAAAGATCTCTTGTCGCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGATCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCAtgc < 2:612139/238‑1 (MQ=255) ccggctgATTGCTCTGCCGAAAGGGGCGGAAGTGGGGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCAt < 1:568344/234‑1 (MQ=255) ccTGCTTATTGCTCTGCCGAAAGTGGCGGAAGTGGCGGAGGAAGAAGTTGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCCGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCAtg < 2:424869/238‑1 (MQ=255) ttgctgATTGGCTTGCCGAAAGTGGCGGAAGTGGCGCAGGAAAGAGTGGTCGAACCGAAAATTGAAAACCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCAtgc < 2:129809/237‑1 (MQ=255) tgctgATTGCTCTGCCGCAAGTGGCGGCAGTGGCGCAGGAAGAAGTGGTCGAACCGAAACATGAAAATCCGCAGCCGGATGGAAAACCGCCCGGC‑GAAACGCCGAAAAACATCTTTTCTCGCCCGGGCTTGCCGGAAATGGCCCACCGGCATTTGC‑CGCTTTACCCGCACCATGAGGAGTTTAAGGGCGGGCACCGGCGCGGTGCGGGGGACACGGAAAACACCACGAGcccgaccctt > 2:741104/1‑230 (MQ=255) gctgATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGaa < 1:601477/175‑1 (MQ=255) gctgATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAAAATTTaa > 2:601477/1‑175 (MQ=255) tGCTCTGCCGAAAGTGGCGGAAGGGGCGCAGGGAGAAGTGGGCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAAATCTCTTTTCTCGCCCGGTATTTCCGGAAATGACCGAGAGGCATTTGC‑CCCTTAACCTGGACATTTGAGAGTTTAAGGGCGAGCAGGAGCGCGTGACGGGCGCAACGGAACACACCGTAGGCACCCAGCTGCTGaacg > 2:728188/1‑237 (MQ=255) ctGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGGGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGag < 1:485359/239‑1 (MQ=255) tGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAgg > 1:952699/1‑174 (MQ=255) tGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGGATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCTGCTGCGCGTGACGGGCGCAACGGGAATCACCGTGAGCACCATGCTGCTGCAAGTGagc > 1:78820/1‑239 (MQ=255) aaGTGGCGGATGTGGCGCAGGAAGAAGTGGCCGAACCAAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGGTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCAtt < 2:78820/239‑1 (MQ=255) gtggAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGGAGGCGGATGAAAAGCCGCTCGGC‑GAAACGCGGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg < 2:715574/237‑1 (MQ=255) cgtaaGTGGCCAAGGAAGAAGGGGTCCAACCGAAAATGGAAAACCCGCAGCCGGTAGAAAAGCCCTACGTC‑GAAACGCTGAACGATCTCTTTTCTTGCCCGGTATTGCCGGAAAGGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGGGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg < 2:157473/235‑1 (MQ=255) cGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCCTTTGC‑CCCTTAACCTGAACATTGAAAGGTTTACGGGCGAGCAGATGCCCGGGCAGGGCGGCACGGGCAACACCGTGAGAACAAAGCGGTCGAAAGGAAGCAGCATTgcaggc > 2:328562/1‑234 (MQ=255) cGGAAGTGGCGCAGGAAGAAGGGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg < 1:263929/238‑1 (MQ=255) cGGAAGTGGCGCAAGAAGAAGTGGTCGAACCGAAAATTGAAAAGCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg < 1:125857/238‑1 (MQ=255) cGGAAGCGGCGAAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAATGCTGAAAGATCTCTTTTCTCGCCTGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACgg < 1:238793/238‑1 (MQ=255) cgcAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGCGAGCGGCATTGACGGCAATCCTaa > 1:84688/1‑238 (MQ=255) aagaagTGTGCGAACCGAAAATTGAAAGTCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTgg < 1:838330/237‑1 (MQ=255) aagaagGGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTGGa < 1:742289/238‑1 (MQ=255) aagaagGGGTCGAACCGAAAATGGAAAATCCGCAGCCGGATGAAAAGCCGCACGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTgg < 1:1085576/237‑1 (MQ=255) agaagTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTGGAc < 2:341854/238‑1 (MQ=255) aggTGGCCGAACCGAAAATTGAAACTCCGAAGCCGGATGAAAAGCCGCTCGGC‑GAAACGGTGAAAGATCTCTTGTCTCGCCCGGTATTGCCGGAAATGACCGACGGGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTGGACGcc < 1:487331/237‑1 (MQ=255) agTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGGGAGCAGCATTGACGGCAATACTAAACTGGACGccc > 1:481473/1‑239 (MQ=255) agTGGGCGAAACGAAAATTGAAAATCCGTAGCCGGGTGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCCCTTTTCGCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTGGACGcc < 2:481473/238‑1 (MQ=255) | GTTGAAGAAGAGGAAGATAGCGGTCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGC‑GAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCCGGAAATGACCGACGTGCATTTGC‑CGCTTAACCTGAACATTGAAGAGTTTAAGGGCGAGCAGCTGCGCGTGACGGGCGACACGGACATCACCGTGAGCACCATGCTGCTGAAAGTGAGCAGCATTGACGGCAATACTAAACTGGACGCCC > NC_000913/4444457‑4444925 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |