Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 501,710 T→C 13.9% E510G (GAG→GGG)  ybaL ← inner membrane putative NAD(P)‑binding transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913501,7100TC13.9% 186.8 / 2.7 72E510G (GAG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
Reads supporting (aligned to +/- strand):  ref base T (47/14);  new base C (2/9);  total (49/23)
Fisher's exact test for biased strand distribution p-value = 3.37e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATT  >  NC_000913/501472‑501935
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
tCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGTCGCGGGCAATAAtct                                                                                                                                                                                                                                   >  1:427896/1‑239 (MQ=255)
               tcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGAtttt                                                                                                                                                                                                                    >  1:752506/1‑239 (MQ=255)
               tcGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGGGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAACTCCACAATCCGGAtttt                                                                                                                                                                                                                    >  2:627716/1‑239 (MQ=255)
                         aTGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGc                                                                                                                                                                                                          >  1:616363/1‑239 (MQ=255)
                                    gcgcGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCCCAATATCCGGATTTTTCGCGCGGGCAGATGCccca                                                                                                                                                                                                >  2:240770/1‑238 (MQ=255)
                                         tCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGGTTTTTCGCGCGGGCAGATGCCACAAtctc                                                                                                                                                                                           >  1:196135/1‑238 (MQ=255)
                                             tttctcccTATATTTTTTTATTTTTTTACTCCCGCCCACCCCCCTTTCCCCCCCCCCCCCCCCCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCCCCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCAccc                                                                                                                                                                                       <  2:616363/233‑1 (MQ=255)
                                                    cctaattttttttATATTTTTCCCTCCGACACCCCCCTTCTCCCCCGCCCCCCCCCCCCCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCCCGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCAt                                                                                                                                                                                <  1:209159/232‑1 (MQ=255)
                                                       cATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCCGATGCCACAATCTCACCCCCTTCATAAc                                                                                                                                                                             >  1:739462/1‑238 (MQ=255)
                                                       cATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAAcc                                                                                                                                                                            >  1:745391/1‑239 (MQ=255)
                                                            ttttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTgg                                                                                                                                                                       >  1:383900/1‑239 (MQ=255)
                                                              ttCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCCCCCGGGCAGATGCAACAATCTAACCCGCTTCATAACCGTTGGGa                                                                                                                                                                     >  2:899230/1‑239 (MQ=255)
                                                                       ttACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCCCAATCTCACCCGCTTCATaa                                                                                                                                                                              >  1:720322/1‑222 (MQ=255)
                                                                               ggCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAAcc                                                                                                                                                                            >  1:837870/1‑216 (MQ=255)
                                                                                         gTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAAtc                                                                                                                                                                                             >  1:225068/1‑189 (MQ=255)
                                                                                          tttACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACAt                                                                                                                                          >  1:769593/1‑239 (MQ=255)
                                                                                               cccGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCCATAATCTCAATATCCGGATTTTTCGCTCGGGCAGATGCCACAATCTCACCCGCTTCATAACCCTTGGGAATCGTCAGGTACACCCATTTTGCACATTCCAg                                                                                                                                     >  2:229335/1‑239 (MQ=255)
                                                                                                  gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCCGact                                                                                                                                  >  1:236851/1‑237 (MQ=255)
                                                                                                  gTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAAATg                                                                                                                                  >  2:93062/1‑239 (MQ=255)
                                                                                                                  gccgGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTCGGAATCGTCAGGACCCGCCATTTTGCACATTCCAAATGCCCAAGttaccaaat                                                                                                                   >  2:628270/1‑232 (MQ=255)
                                                                                                                              tCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACACGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCCTTTTGCACATTCCAGATTCGCCAGTTGCATAATTTCttcgttcc                                                                                                        >  1:207816/1‑237 (MQ=255)
                                                                                                                                  gctcttCCAGCCTGGCACGGGCAGTTGCCCGCTCCGCCCACTCTTCCTGATTCGCCCCAAGTTCGGGCATATACGCCCCTTCATCGTAATAATGGGCGCGGGAAATAATCCCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   <  2:1013359/235‑1 (MQ=255)
                                                                                                                                  gcattgccaccaTGGGCCGGGGGATTCCCCGCCCGCCCATAACTACCTGATTCGCACCACTTTCGGTGATATACGCCACTTCATCGTCATAAGGGGCGCGGGCAATAATCCCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   <  2:616092/233‑1 (MQ=255)
                                                                                                                                  gcagTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   <  1:93062/239‑1 (MQ=255)
                                                                                                                                  cggtttCCCGCTTGGTCGGGGCGTTTTCCCGCCCGCCCTTCACTACCGGATTCGCCCCACGTTGGGTAATAACCGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   <  2:530040/235‑1 (MQ=255)
                                                                                                                                  ccctttCCACCCTGGTCGGGGCGTTTTCACCCCCGCCCATCCCTCCCTGATTCCCCCCCCGTTCGGTTATATACCCCCCTTCATCGTCATAATGGGCGCGGGCAATAATCCCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   <  2:1080074/235‑1 (MQ=255)
                                                                                                                                       tCCACCCTGGTACGGGCGTTTTCCCGCTCGCCCATCACTCCCTGATTCGCACCACGTTCGGTGATATACGCCACTTCCTCGGCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGcc                                                                                              <  2:752506/239‑1 (MQ=255)
                                                                                                                                             aTGGCACGGGCGTTTTCACGCCGGCCCATCACTTCCTGATTCTCTCCACGTTCGGTGATATACGCCACTTCAGCGTCATAATGGGCGCGGGCAATAATCTCAATAGCCGGATTTGTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATa                                                                                         <  1:445282/238‑1 (MQ=255)
                                                                                                                                                gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTgc                                                                                     >  1:653522/1‑239 (MQ=255)
                                                                                                                                                gTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAAAACTg                                                                                      >  1:751786/1‑238 (MQ=255)
                                                                                                                                                      gCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATCCTCCACggg                                                                                >  1:1059801/1‑237 (MQ=255)
                                                                                                                                                      gCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCCCggc                                                                                >  1:220914/1‑237 (MQ=255)
                                                                                                                                                         aTTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGAcac                                                                             >  1:485689/1‑236 (MQ=255)
                                                                                                                                                          tttCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATa                                                                                         >  1:928684/1‑225 (MQ=255)
                                                                                                                                                           ttCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATTGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAAAACTCCGCGGCcccccc                                                                          >  2:110460/1‑237 (MQ=255)
                                                                                                                                                                 ccccccccTTCCCTCCCTGGTTCGCCCCCCGTTCGGTGATAAACGCCATTTCACCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGGGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGctct                                                                     <  2:751786/234‑1 (MQ=255)
                                                                                                                                                                     gCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGc                                                                  >  1:88952/1‑238 (MQ=255)
                                                                                                                                                                      cccATCCCCACCGGTTTCGCACCCCGTTCGGGGATATACGGCCTTTCATCGGAAAAATGGGGGCGGGAAAGAATCTCAAGATCCGGATTTTTCGCGCGGGAAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAg                                                                <  2:915770/239‑1 (MQ=255)
                                                                                                                                                                         aTCCCTACCTGATTCGCACCACGTTCGGTGATATACCCCCCTTCATTGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCtc                                                             <  1:110460/239‑1 (MQ=255)
                                                                                                                                                                          tCACTACCTGATTCGCACCCCGTTCGGGGATATACGCCACTTCCTCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   <  2:599863/200‑1 (MQ=255)
                                                                                                                                                                               aCCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCCCGCCCCCGCAGCCCACCaaa                                                       >  2:131832/1‑238 (MQ=255)
                                                                                                                                                                                ccgtaTTCCCCCCACGTTCGGTTATATCCCCCACTTCATCCTCATAAGGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGGAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAaca                                                      <  1:904142/235‑1 (MQ=255)
                                                                                                                                                                                ccTGTTTCGCCCCCCTTGCGGTGATTTACGCCACTTCCCCGCCAAAATGGGGCAGGGCAATAACCCCAAAACCCGGATTTTGCGGGCGGGAAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCCCATCAaca                                                      <  2:769593/239‑1 (MQ=255)
                                                                                                                                                                                ccTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAac                                                       >  1:788461/1‑238 (MQ=255)
                                                                                                                                                                                   gATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATCCTGCGCGGCCCCCGCGCTCTCGCAGCCCATCAACACgg                                                   >  2:904142/1‑239 (MQ=255)
                                                                                                                                                                                    aTTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATa                                                                                         >  1:599863/1‑200 (MQ=255)
                                                                                                                                                                                        gCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCgcgc                                                                        >  2:1001196/1‑213 (MQ=255)
                                                                                                                                                                                        gCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCgcgc                                                                        <  1:1001196/213‑1 (MQ=255)
                                                                                                                                                                                          accacGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACAGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTATTCGTTCGCCGCATGGCCCAATACTGCGCGGACCCCGCGCCCTCCCCGCTCACCAAAACGGGTTCgg                                             >  2:405075/1‑237 (MQ=255)
                                                                                                                                                                                          accacGTTCGGTGATATACGCCACTTCATCGTCATAATAGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGTGGGCAGATGCCACAATTTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCAAGATGCCCCAGTTGCATAATTTCCTCGTTCGACGCATTGCCCCAACCTTCCCGGACCCCGGGCTTCCCCACCTCCTCAACACGGGtcctga                                            >  2:904794/1‑234 (MQ=255)
                                                                                                                                                                                              cGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGACCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTGGCCGCATTGCCCAATACTCCCCGGGCCCCGCCCTCTCGCCGCCCCTCAACACGGGTTCGTGCCg                                         >  2:24236/1‑238 (MQ=255)
                                                                                                                                                                                                 tCGGTGAAATACGCCACTTCCACGTCATAATGGGCTCGGGCAATAATCCCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtct                                      <  1:1014342/238‑1 (MQ=255)
                                                                                                                                                                                                    gTGATATACGCCACTTCATCGTCATAATGTGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTTGCCGCATTGCCCAATACTGCGCGGACCCCGCGCCCTTGCACCTCACCACAACGGGTTCGTGACGCTTCaa                                   >  1:279166/1‑238 (MQ=255)
                                                                                                                                                                                                               cACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCCGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTCCTTGACGTCTCAATCCcccccacgg                       >  2:596587/1‑236 (MQ=255)
                                                                                                                                                                                                               cACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCAccaccacc                        >  1:1019204/1‑236 (MQ=255)
                                                                                                                                                                                                               cACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCACCg                       >  1:188120/1‑239 (MQ=255)
                                                                                                                                                                                                                ttttCATCGGCAAAAGGGGCGGGGGAAAATACCCAAATATCCGGTTTTTTCGCGCGGGGAGATTCCAAAATCTCCCCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg                      <  2:1059801/237‑1 (MQ=255)
                                                                                                                                                                                                                attttctgttCATAATGGGGGCGGGAAATATACCCAATATCCGGATTTTCCAGGCGGGCAGATGCCACAATCGCAAACTGTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTGCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCgg                      <  2:207816/230‑1 (MQ=255)
                                                                                                                                                                                                                 cTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGca                                                                                                                                             >  1:242182/1‑119 (MQ=255)
                                                                                                                                                                                                                 cTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   >  1:115861/1‑161 (MQ=255)
                                                                                                                                                                                                                 cTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAAAACGGGTTCGTGACGTCTCAATCACCACCAGCGGa                     >  1:635471/1‑239 (MQ=255)
                                                                                                                                                                                                                 cTTCATCGTCATAATGGGCGCGGGCAATAATCCCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCa                                                                                                   <  2:115861/161‑1 (MQ=255)
                                                                                                                                                                                                                 cTTCATCGTCAAAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGca                                                                                                                                             <  2:242182/119‑1 (MQ=255)
                                                                                                                                                                                                                  ttCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCCCCGCTCATCAACACGGGTTCCTGACGCCCCAACCCCCACCACCGGaa                    >  2:561503/1‑239 (MQ=255)
                                                                                                                                                                                                                      tCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGTTCATCAACACGGGTTCTGGACGTACCAATCCCCCCCCGCGGaaaacc                >  2:549334/1‑235 (MQ=255)
                                                                                                                                                                                                                       cGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa               >  1:47932/1‑239 (MQ=255)
                                                                                                                                                                                                                        gTCATAAGGGGCGCGGGCAATAAGCTCAATACCCGGATTTTTCGCGCGGGCAGATGCCACAAGCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCa               <  1:740021/238‑1 (MQ=255)
                                                                                                                                                                                                                                  gcgcGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTAGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCCCCGCTCATCAACACGGGTTCGGGACGGCTCAATCCCCCCCAGCGGACTATCCGCGGCGAGcca    >  2:740021/1‑237 (MQ=255)
                                                                                                                                                                                                                                   cgcgGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCAtt                                                                                                 >  1:510289/1‑145 (MQ=255)
                                                                                                                                                                                                                                   cgcgGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGCCGTCTCAATCACCACCACCGGAATATCAGAGGCGAGCAAt   >  1:800107/1‑239 (MQ=255)
                                                                                                                                                                                                                                   cgcgGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAAAGGCGGGCAAt   >  1:192664/1‑239 (MQ=255)
                                                                                                                                                                                                                                   cgcgGGCAATAATCCCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCAtt                                                                                                 <  2:510289/145‑1 (MQ=255)
                                                                                                                                                                                                                                     cgGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAAtt  >  1:30369/1‑238 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 
TCTGGCATTTGGCTCTCGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACATATTTTTCATATTTTACATCCGGCAACCACCGTTTACCCCGTCACCACCTCACCCGCCGGTGGCGTTTCCAGCAGTTCCAGCATGGTACGGGCGATTTCACGCTCGCCCATCACTACCTGATTCGCACCACGTTCGGTGATATACGCCACTTCATCGTCATAATGGGCGCGGGCAATAATCTCAATATCCGGATTTTTCGCGCGGGCAGATGCCACAATCTCACCCGCTTCATAACCGTTGGGAATCGTCAGGATCAGCCATTTTGCACATTCCAGATGCGCCAGTTGCATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGAGCAATT  >  NC_000913/501472‑501935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: