Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 514,789 T→C 22.3% E24G (GAG→GGG)  ybbJ ← inner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913514,7890TC22.3% 65.1 / 2.1 41E24G (GAG→GGG) ybbJinner membrane protein; stimulator of the QmcA suppressor of ftsH‑htpX
Reads supporting (aligned to +/- strand):  ref base T (25/5);  new base C (4/6);  total (29/12)
Fisher's exact test for biased strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCA  >  NC_000913/514557‑515022
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          
aaaaCGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑CAATCACCGCTGCCACGCCCCTCCACAACAACTAACCA‑CTTCCCCCCACCATCCCGGCt                                                                                                                                                                                                                                       >  1:522279/1‑238 (MQ=255)
       cGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGACTATCGGCTGCcagcag                                                                                                                                                                                                                               >  1:134569/1‑239 (MQ=255)
                            cgcTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCCAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCCGCCGCCAGCAGCAAACCGCCCAAACTGAACCa                                                                                                                                                                                                          >  2:217872/1‑239 (MQ=255)
                                           cTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGACCCAGAAAATATGTGGATg                                                                                                                                                                                           >  1:804257/1‑239 (MQ=255)
                                           cTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGCCTGCCAGCAGCAAACCGCCGAGACTGAACCAGCAAATATGCGGATg                                                                                                                                                                                           >  1:428177/1‑239 (MQ=255)
                                           cTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGC‑GAATCCCCGCTGCCACGCCGCCCCCCACCAAATAACCA‑TTCCCGCCCAGACTCTCGGCTCCCACCAGCAACCCCCCCCGACTGAGCCAGAAAAAATTGGGATg                                                                                                                                                                                           >  2:417200/1‑239 (MQ=255)
                                                    tgCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATTGGGATGAACGACCAt                                                                                                                                                                                  >  1:86783/1‑239 (MQ=255)
                                                      cTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTa                                                                                                                                                                                >  1:149219/1‑239 (MQ=255)
                                                         ttGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACACCAAATAACCA‑TTTCCGCCCAGCATCCCGGCTGCCAGCAGCAAACCGCCGGGACGGAGCCCGAAAATATGTGGCTGAACGACCATTAAc                                                                                                                                                                              >  1:398394/1‑238 (MQ=255)
                                                                cgcACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAAAATGTGGATGAACGACCATTAACTCcatcat                                                                                                                                                                      >  1:218305/1‑239 (MQ=255)
                                                                                                  aGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGCAAATATGTGGATGAACGACCCTTAACCCCACCCTGGCTGAGCCCGTTTGTTGGCGCTGCCTTTCAcc                                                                                                                                     >  2:83756/1‑238 (MQ=255)
                                                                                                   ggcggcGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCCCGGCTGCCAGCAGCAAACCGCCAGGACGTAGCCAGCAAATATGGGGTTGAAAGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGGCTTTCACCa                                                                                                                                    >  2:1072708/1‑238 (MQ=255)
                                                                                                          agcagcGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TATTCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCCCGGCTGCCACCAGCAACCCGCCGAGCCTGAGCCAGAAAAAATTGGGCTGGACGACCATTACCCCCATCATGGCTGGGTCCGTTTTTTTGCCCGGCCTTTcccccacccgcca                                                                                                                            >  2:894143/1‑230 (MQ=255)
                                                                                                                         gCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCACCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCCGAAAATAGTTGGATGAACGACCATTACCTCCATCTTGGCTAAGTCCGTTTGTTGGCCCGGTCTTTACCCAACCCGGCAAACCCGC‑CAATcaa                                                                                                             >  2:614274/1‑236 (MQ=255)
                                                                                                                                 ttccccCTGCCCCCCCCAACCCCAGCGGCCCCAGCCAGCCCCCAAGGCCGG‑TAACCCCCGCTGCCCCGCCGCCCCACAACAAATACCCA‑TTTCCGACCAGCATCGCGGCTGCCAGCAGCAACCCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCa                                                                                                     <  1:614274/237‑1 (MQ=255)
                                                                                                                                   ccccTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑GAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGCCTGCCAGCAGCACACCGCCGAGACTGAGCCAGAAAATATGTGGCTGAACGACCATTAACTCCATCATGGCTGAGTCCGGTTGTTGGCCGCGTCTTTCCACAGCCCGGCAACCCCGGCCACACGACCCCacac                                                                                                     >  2:69252/1‑234 (MQ=255)
                                                                                                                                        tGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGCCCATTAACTCCATCATGGCTGGGTCCGTTTGTTGGCGCTGTCTTTCACCAGCCCGGCAATCCCGG‑CAATCCACCCCATCAggcggcgg                                                                                             >  2:395006/1‑237 (MQ=255)
                                                                                                                                        tGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCACCAAACCGCCGAGCCTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCCTGGCTGCGTCCGTTTGTTGGACCTGTCTTTCCCCACCTCGGCCATCCCGC‑CAACCAACCCCAACACgccaccg                                                                                             >  2:471255/1‑234 (MQ=255)
                                                                                                                                        tGCCACTACCAA‑CCCAGCGGCACCAGCCAGACCACCAGACCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGTAAATTTGTGGAAGAACGACCATTAAATCCCTAATGGCTGTGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGC‑AAATCGACCCCCTCAAgttgct                                                                                              >  2:768279/1‑238 (MQ=255)
                                                                                                                                          ccccccccAA‑CCC‑GCGGCCCCCACCCCACCCCCAGGGCGGCTAACCCCCGTGCCCGCGCCCCCCCAAAAAAAATACCCA‑TTCCCCCCCGGCATCCGGTCGGCCAGCAGCAACCCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGc                                                                                           <  2:218305/234‑1 (MQ=255)
                                                                                                                                                                cagcaagCCCACAAGGCCGG‑AAACCCCCGCGGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGAATCCCGGCTGCCAGCAACAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTGGGAGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCactac                                                                      <  1:273446/239‑1 (MQ=255)
                                                                                                                                                                  gccagACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGGGTCCGTTTGTTGGCGCTGCCTTTCACCAGCTCGGCAATCCCGC‑AACTCGACCCCATCCGGCTCCGGGCCTCTAATGGCACAACCACAACtt                                                                    >  2:374724/1‑239 (MQ=255)
                                                                                                                                                                                       acccccccTGCCCCGCCCCTCCAAAAAAAACACCCC‑TTCCCGCCCAACCTTCCGGCTGCCAGCAGAAAACCGCCGAGACTGGGACAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCg                                                <  1:954693/232‑1 (MQ=255)
                                                                                                                                                                                         ccccgcgcccACGCCCTCCCAAAACAAAAAACCA‑TTTCCGCCCAGCATCCAGGCGGCCAGAAGAAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGAt                                              <  1:328852/231‑1 (MQ=255)
                                                                                                                                                                                                 caccgcccgtCCACAAAAAAAAACCA‑TTCCCGCCCCGCTCCCCGGCTGCCAGCCGCAACCCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTACCTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTa                                      <  1:103098/230‑1 (MQ=255)
                                                                                                                                                                                                      ccgcTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGACAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCACCACTCCTTTGCTGTTCCTGGAGGAACCGCTCTGCGGTAACga                                  >  2:924079/1‑237 (MQ=255)
                                                                                                                                                                                                                  aaaCAACCACTTCCCGCCAAGCCTGTCGGCTGCCGCAATCACCCCGACGAGCCTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTAttt                      <  1:1045080/239‑1 (MQ=255)
                                                                                                                                                                                                                   aaTAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCCCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCAGCTGTAACGCTTCGGGGTATTTcg                    >  2:386346/1‑238 (MQ=255)
                                                                                                                                                                                                                    aTAACGA‑TGTCCGCGCGGCATCTCGGCTGCCAGCACCAATCCGCCGAGACTGAGCCAGAAAATATGGGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGGCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTg                   <  1:211376/239‑1 (MQ=255)
                                                                                                                                                                                                                    aTAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCGGTAACGCTTCGGTGAATTTCt                    >  1:553726/1‑238 (MQ=255)
                                                                                                                                                                                                                      aaCCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGCCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACACCTTTGCTGTTACTGGAGGAACCCATCTGCTGTAACGCCTCGTAGTATTTCTgc                  >  1:1041075/1‑238 (MQ=255)
                                                                                                                                                                                                                         cA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATg                                                                                   >  1:557211/1‑170 (MQ=255)
                                                                                                                                                                                                                          tttttCCCCCCAGTATTTCGGCTACCAGCAACGACCCGCCGAGACTGAGCAAGGAAATTTGTGGATGAACGCCCATTAACCCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTGCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTa              <  2:12760/237‑1 (MQ=255)
                                                                                                                                                                                                                            tttCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGgcg                                                                                                                                                 >  2:211912/1‑107 (MQ=255)
                                                                                                                                                                                                                            tttCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGgcg                                                                                                                                                 <  1:211912/107‑1 (MQ=255)
                                                                                                                                                                                                                                cGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGGGTATTTCTGCGACTCGAagt        >  1:670429/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCTTCAGGCTGCTGGCCCCTAATGGCATCAGCCCTCCTTTTCTGTTACTGGAGGAACCCATCGGCTGTAACACCTCGGTGTATTTCTGCGTTaagaagaa       >  2:798837/1‑237 (MQ=255)
                                                                                                                                                                                                                                  cccAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACCCTTCGGTGTATTTCTCCGCTCCGaagaag      >  2:548131/1‑239 (MQ=255)
                                                                                                                                                                                                                                   ccAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCATGCTGCTGGCCGCTAATGGCATCATCCCTACTTTGCTGTTACTGGAGTAACCGATCTGCTGTAACGCTTCGGGGTATTTCTTCCCTACAAagtcgt     >  1:1061987/1‑239 (MQ=255)
                                                                                                                                                                                                                                      gCATCCCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCa  <  1:395006/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aaTCTCGGTTCCCAGCAGAAAGCCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCa  <  1:894143/237‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                          
AAAACGTCGGCCAATCAGCTGCTGCCCGCGCTGGTTTAAATGACTATCGCTGTGCTTTTGTTCGCGCACCCGCCGCGACAACCATTTCCACCACAGCCAGGCGGCGAGCAGCGTCAGGATGGCAAACATTACCCCTTGCCACTCCCAA‑CCCAGCGGCACCAGCCAGACCACCAGGCCGG‑TAATCACCGCTGCCACGCCGCTCCACAACAAATAACCA‑TTTCCGCCCAGCATCTCGGCTGCCAGCAGCAAACCGCCGAGACTGAGCCAGAAAATATGTGGATGAACGACCATTAACTCCATCATGGCTGAGTCCGTTTGTTGGCGCTGTCTTTCACCAGCTCGGCAATCCCGG‑CAATCGACCCCATCAGGCTGCTGGCCTCTAATGGCATCATCACTACTTTGCTGTTACTGGAGGAACCGATCTGCTGTAACGCTTCGGTGTATTTCTGCGCTACGAAGTAGTTCA  >  NC_000913/514557‑515022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑