| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 559,176 | T→A | 16.8% | V68E (GTA→GAA) | sfmC → | putative periplasmic pilus chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 559,176 | 0 | T | A | 16.8% | 115.5 / 5.0 | 49 | V68E (GTA→GAA) | sfmC | putative periplasmic pilus chaperone |
| Reads supporting (aligned to +/- strand): ref base T (34/6); new base A (2/7); total (36/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGTCAGGAATACACAAGGCGTATTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGAA > NC_000913/558945‑559414 | ccGTCAAGAATTCACCAGGCTTTTTAACTATTATGACTAAAATAAAGTTAGTGATTCTCATTTTATTTTATTTAATCATTTCGGCCAGCGCCCAAGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTaaaagaa < 2:878277/239‑1 (MQ=255) aTACCCAAGGCGTTTTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGtcatt < 2:642786/238‑1 (MQ=255) caAGGCGTATTAACTATGATGACTAAAATAAAGTTATTGATTCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTcatcat < 1:935376/239‑1 (MQ=255) aTTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGtcatt < 1:234665/225‑1 (MQ=255) atgaCTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAACTAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGGGTAAAAGAAAAGTCATTCATCATTACACCGCCACtgtttgt > 1:510862/1‑239 (MQ=255) tAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGCATGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTAAA‑TTCGTGGATTGAAAACAGCAGCGGTGTCAAAGAAAAGcaattca > 1:337849/1‑212 (MQ=255) aaTAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGCCTGCGGTATTGATTAGACATAGCCATACCAATGAGCGCTTTCTGGCCAA‑TTCGTGGATTGAAAACAGCAGCGCTGTAAAAGAAAAGTCATTCCTCATTACCCCGCCACTGTTTGTTAGAGaa > 2:218412/1‑238 (MQ=255) aGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTAATTAATCATTACACCGCCACTGTTTGTTAGGAAACCCaaa > 2:580641/1‑239 (MQ=255) tatttaATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGtattat > 1:512416/1‑239 (MQ=255) aaTCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAATCGAAAAAACTTTGCGAATTATTTac > 1:1012316/1‑238 (MQ=255) tCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTAGACCGCCACTGTTTGTTAGTGAACACAAAAACGAAAAAAATTTACTTATAATTTACAcc > 2:462499/1‑239 (MQ=255) tCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCCCCACCGTTGTTTAGTGAACCCAAAAACAAAAAAACATTCGGAATTATTTAAAcc > 2:422718/1‑239 (MQ=255) tCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACAGCACCGTTTTGTTAGTGAACCCAAAAGCAAAAATACTTTGCGCATTATTTAAACAGGTcc > 2:396844/1‑239 (MQ=255) cccccccctggctgcGGGAGGTGTCGTATGGGGTGCCGGGTTTATTATTTCCCCCCATGATGAAAACCATGCGGCGGTATGGATTAAAAATAGCCATCCAAAGGAGCCTTTTCTGGTAAA‑TTCGTGGATTGAAAACAGCAGCGGTAAAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGc < 2:541189/229‑1 (MQ=255) aTGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTGAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCCAGTTCTGGTCAA‑TTCGAGGATTGAAAACAGCAGCGGTGAAAAAGAAAAGTCATTCATCATTACACCTCAACTGTTTGTTAGTGAACCCAAAAGCGAAAAACCTTTGGGAATACTTTAAACAGGCCCACCCCGGgaagca > 2:549988/1‑239 (MQ=255) aTGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTACAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCCCTGgcagca > 1:685708/1‑239 (MQ=255) aTGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATCATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGGGAAAATACTTTGCGaattat > 1:723445/1‑214 (MQ=255) cGGAGGCACCGCATGAGGGACCACGCTTATTATTTACCCCGCTGATGCAAAACAGACTGCGGTAGGGATTAGAAATAGCAATACAAATGAGCGCTTTCTGGACAA‑TTCGGGGATTGAAAACAGCAGCGGTTAAAAAAAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTg < 1:218412/239‑1 (MQ=255) aTCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCACCGGGGACAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCAAAAAGCGAAAATACTTTTGGTATTTTTTAAACCGGTCCCCCGCTGgcagcaactccaggatc > 2:946343/1‑225 (MQ=255) ggTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATCCTTGGCGTATTATTTAAACCGGTCCACCCCTGGCAGCAGATCGGGAGTTTTTGTTTTGGAt > 2:581827/1‑239 (MQ=255) gTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTTGGTATTATTTACACCGGTCCACCGCGGGCGGCCAACCGTGAGTCTCTGTTTTGGGTg > 2:23258/1‑239 (MQ=255) ggggttttttttacCCCGTTGTTGCTAAACAGATTGCGGTATGGATTAAAAAAAGCCATACAAATGGGCGTTTTCTGTTCAA‑TTCGGGGATTGAAAACAGCAGCGGTGAAAAAGAAAAGTCATCAATATTTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGtt < 1:422718/230‑1 (MQ=255) attattTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAgtc > 1:539917/1‑237 (MQ=255) tatCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTTAACCCAAAAGCGAAAATACTTTGCGACTTATTTACACCGGTCACCCGCTGGCGGAAGATCGTGGGTTCCTGTTCTGGATGAATGTTAAAAGGATc > 2:663237/1‑237 (MQ=255) atCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCt > 1:721949/1‑239 (MQ=255) atCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCt > 2:310216/1‑239 (MQ=255) cccGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTc > 1:176396/1‑239 (MQ=255) cTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTa > 1:1059627/1‑239 (MQ=255) tGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAg > 1:288935/1‑239 (MQ=255) aaaaCAGACGGGGGTATGGATTAAAAATAGCCATCCCAATGAGCGTTTTCTGGTCAA‑TGCGTGGATTAAAAACAGCAGCGTTGTAAAAAAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATaaaa < 1:23258/238‑1 (MQ=255) gACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGGATTATTTACACCGGTCCACCGCGTGCAGAAGATCGGGAGTCTATGTTCTGGATGAATGTTAAAACGATCCCTGCGGGAGATAAAAAAGCAt > 2:474820/1‑239 (MQ=255) tGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTAAACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTTTGGCTGAATGTTAAGACGACCCCTCCGGGAGATAAAAATGCAttaa > 2:563696/1‑237 (MQ=255) tttgtataaaaaaTAGCCAACCAAATGGGTGTTTTTGTGTAAA‑TTCGGGAATTAAAACCAGAAGGGGTAAAAAAAAAAGTTCATTCACCATTACCCCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACgg < 2:288935/228‑1 (MQ=255) tttGGATTAAAAAGAGCCAAACAATGGAGCGCTTTCT‑GTCAATTTCGTGGATTGAAAACGGCAGGGGTGAAAAAAAAAAGTAATACATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACgg < 1:751638/236‑1 (MQ=255) aTTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGaa > 1:997208/1‑239 (MQ=255) aTTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCAACCGCTGGCAGCAGATCGTGAGTCTCTATTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGAATTGAAAGGCCGGaa > 1:389613/1‑239 (MQ=255) aGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCa > 1:1015822/1‑239 (MQ=255) gCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTGGATAAAAATGCATTGAACGACAGGAATGTTTTGCaa > 1:104855/1‑239 (MQ=255) gCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCAACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGAACCCTTCGGTCGATAAAAATGCATTGAACGGCAGGGAATTTTTGCaa > 2:154567/1‑239 (MQ=255) tGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGTTAGGTCAAAATGCATTGAACGGCAGGAATGTTTTGCAAAGGGCGAttt > 2:657496/1‑239 (MQ=255) cgcTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAAGGCATTTAACGGCAGGAATGTTTTGCAAATGGGGATTTTATc > 2:395473/1‑239 (MQ=255) tttCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGAATTGAACGGCAGGAAATTTTTGCAACTGGGGTTTTTATcccc > 2:575809/1‑236 (MQ=255) tgtggaaaa‑TTTGTGCATGAAAAACATCAGGGGTAAAAAAAAAAAGTCATTCATCTTTCCTCCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCa < 1:563696/232‑1 (MQ=255) gttttttttgtgtATTAAAAACAGCGGGGGTGAAAAAAAAAATGTAGTCACCATCACCCCGCCCCTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGa < 2:510862/230‑1 (MQ=255) gTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGaa > 2:876431/1‑239 (MQ=255) a‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGCAAGGAATGTTTTGCAACTGGCGATTTTACCGCGCATGAAatta > 1:121291/1‑239 (MQ=255) ggATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGCTCCCTTCGGTAGATAAAACTCCATTGAACGGCAGGAATGTTTTGCAACTGGGCATTTTAACGCGCATGAAATTAtttat > 1:439891/1‑236 (MQ=255) cGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAAc > 2:353194/1‑172 (MQ=255) cGGTGTAAAAAAAAATTCATTCACCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAAc < 1:353194/172‑1 (MQ=255) tAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGaa > 1:665045/1‑239 (MQ=255) | CCGTCAGGAATACACAAGGCGTATTAACTATGATGACTAAAATAAAGTTATTGATGCTCATTATATTTTATTTAATCATTTCGGCCAGCGCCCATGCTGCCGGAGGGATCGCATTAGGTGCCACGCGTATTATTTATCCCGCTGATGCTAAACAGACTGCGGTATGGATTAGAAATAGCCATACCAATGAGCGCTTTCTGGTCAA‑TTCGTGGATTGAAAACAGCAGCGGTGTAAAAGAAAAGTCATTCATCATTACACCGCCACTGTTTGTTAGTGAACCCAAAAGCGAAAATACTTTGCGTATTATTTACACCGGTCCACCGCTGGCAGCAGATCGTGAGTCTCTGTTCTGGATGAATGTTAAGACGATCCCTTCGGTAGATAAAAATGCATTGAACGGCAGGAATGTTTTGCAACTGGCGATTTTATCGCGCATGAAATTATTTCTCCGTCCAATTCAATTACAAGAA > NC_000913/558945‑559414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |