Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 759,427 A→G 12.5% E241G (GAG→GGG)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913759,4270AG12.5% 274.5 / 2.0 112E241G (GAG→GGG) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base A (51/43);  new base G (4/12);  total (56/56)
Fisher's exact test for biased strand distribution p-value = 5.58e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 112 total aligned reads displayed.

ACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAC  >  NC_000913/759192‑759662
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            
aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAACTTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGGGACGCGTTAATCCCGATGCTTAACGAgat                                                                                                                                                                                                                                           >  1:1000056/1‑239 (MQ=255)
aCCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAgat                                                                                                                                                                                                                                           >  1:648413/1‑239 (MQ=255)
    aCTGCGGCCCGATTGGTGCCGAGTATATGCACA‑TACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGACGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGGGACGCGGTAATCCCGATGATTAAAGAAATGAAcc                                                                                                                                                                                                                                      >  2:1071384/1‑239 (MQ=255)
      tGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATcc                                                                                                                                                                                                                                      >  1:691545/1‑238 (MQ=255)
         ggCCCGATTGGTTCCGAGTATAGGCACATTACCAGCACCGAAGAAAACCGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑cc                                                                                                                                                                                                                                  <  1:1076077/238‑1 (MQ=255)
         ggCCCGATTGGTCCCGAGTATCGCCACATTACCAGCACCGAAGAAAAACGCTGGATCAAACAGCGTATCGAGTCTGGTCGCCGGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑cc                                                                                                                                                                                                                                  <  1:180001/238‑1 (MQ=255)
                    tGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACa                                                                                                                                                                                                                      >  1:597367/1‑239 (MQ=255)
                           tataTGCCCATTACCAGCACCGAAGAAAACCGCGGGACCCAACAGCGTACCGAGTCGGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACg                                                                                                                                                                                                                               <  1:1081468/223‑1 (MQ=255)
                            tatacccaaTTACCAGCACCGAAGAAAAACCTGGGACCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCAc                                                                                                                                                                                                               <  1:193304/230‑1 (MQ=255)
                            atatGCACATTCCCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTAGCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTAACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCAc                                                                                                                                                                                                               <  1:148977/238‑1 (MQ=255)
                              atGCACATTACCATCACCGAAGAAAAACGCTGGCTCCAACAGCGTATCAAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTCAGCGAA‑CTGA‑CCGCCCCTGAAGGTCTTGAACGTTCCCTCGGAGCAAAAATCCCTGGCGCAAAAAGCTTCTCG‑CTGAAAGGCGGTGACGCGTTAAACCCGGGCCTTAAGGACAAGAACCG‑CCCCGCTGGTAACACCCGCcccc                                                                                                                                                                                                             >  2:870362/1‑238 (MQ=255)
                                   acttcccAGCCCCGAAAAAAACCGCTGGAACAAAAAGCGTATCGAGTCGGGTCGCTGCATTGTCAATAGGGAAGAAAAAAAACC‑TGTCTTAAGCAAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAg                                                                                                                                                                                                       <  2:1000056/234‑1 (MQ=255)
                                    aTTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGAACCCGCGAAGt                                                                                                                                                                                                      >  1:35553/1‑239 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑cc                                                                                                                                                                                                                                  >  1:481020/1‑210 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTg                                                                                                                                                                                                     >  1:784644/1‑239 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGGGACGCGTTACTCCCGATGCTTAAAGAGAGGATCCG‑CCACGCGGGCAACAGCGGCACCCGCGAAGTg                                                                                                                                                                                                     >  1:31649/1‑239 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGCCGGGGACGCGTTAATCCCGATGCTTAAAGAGATGATCCC‑CCACGCGGGCAACAACGGCACCCGCGAAgag                                                                                                                                                                                                     >  2:91247/1‑237 (MQ=255)
                                     ttACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGCCGCAAAACGCTTCTCG‑CTGGAAGGCGGGGACGCGTTAATCCCGAGGCTTAAAGAGATGATCCA‑CCCCGCTGGCCACAGCGGCACACGCGAAggg                                                                                                                                                                                                     >  2:213087/1‑237 (MQ=255)
                                           gCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CGGGAAGTCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACCCTGGCACCAGCGGCACCCGCCAAGTGGGtctc                                                                                                                                                                                               >  1:591704/1‑239 (MQ=255)
                                            cACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGATATGATCCG‑CCACCCTGGCAACACGCGCCCCCGCGAAGGGGGtccag                                                                                                                                                                                              >  2:1015213/1‑236 (MQ=255)
                                             aCCGAAAAAACACGCTGAACCCAACGGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGAGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTtct                                                                                                                                                                                                <  1:480230/236‑1 (MQ=255)
                                              ccGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCggg                                                                                                                                                                                            >  1:970485/1‑239 (MQ=255)
                                              ccGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCggg                                                                                                                                                                                            >  1:567276/1‑239 (MQ=255)
                                                gaagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCCCGAATGGGTTCTCGGGAt                                                                                                                                                                                          >  1:716132/1‑239 (MQ=255)
                                                gaagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACA‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTACAGGGATGATCCG‑CACCGCTGGCAAAAGCGGCCCCCGCGAAGTGGTTCTCggggg                                                                                                                                                                                          >  2:416285/1‑237 (MQ=255)
                                                 aagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGGGATGATCCG‑CCACGCTGGCAACAGCGGCCCCCGGGACGGGGTTCCCgggggg                                                                                                                                                                                         >  2:452813/1‑236 (MQ=255)
                                                 aagaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATg                                                                                                                                                                                         >  1:789932/1‑239 (MQ=255)
                                                  agaaAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTAAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATgg                                                                                                                                                                                        <  1:57378/239‑1 (MQ=255)
                                                              gATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTTTCG‑CTGGAAGGCGGGGACGCGTTAATCCCGATGCTTCAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCCCCCGCGAAGTGGTTTTCGGGATGGCGCACCCTgggc                                                                                                                                                                            >  1:46475/1‑237 (MQ=255)
                                                                   aaCAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGGCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGCAAGGCGGTGACGCGTTAATCCCAATGCTTAAAGGGATGATCCG‑CCACGCTGGCAAACGGGGCCCCCGCCCAGTGGGTCCGGGGAGGGGGcccccggtgcgccga                                                                                                                                                                       >  2:232414/1‑228 (MQ=255)
                                                                    aacgCGTAGCGAGTCTGGTCGGCCGACTTCCAAGAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGG‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtga                                                                                                                                                                      <  1:61509/236‑1 (MQ=255)
                                                                        cGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATCTTTAAAGGGATGATCCG‑CACCGCTGGCAAAAACGGCACCCGCGAGAGGGTTCTCGGGACGCCGCACCCGGGACGCCtgaacgg                                                                                                                                                                  >  2:243986/1‑238 (MQ=255)
                                                                         gtttCGATTCTGGGCGCGCGGTTTTCAAATGCGAAGGAAAAAAACG‑CTTCTAAAGCACA‑CTGC‑CCGCCGCTGAAGGGCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtg                                                                                                                                                                 <  2:35553/236‑1 (MQ=255)
                                                                         gTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAACCG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCATAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgt                                                                                                                                                                  <  1:128355/238‑1 (MQ=255)
                                                                          tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGGTGGCGCCCCGGGGGCGGCtgaacgggc                                                                                                                                                                >  2:400312/1‑239 (MQ=255)
                                                                          tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGGTCTCGGGATGGCGCCCCGTGGCCGTCtgaacgcgc                                                                                                                                                                >  1:284387/1‑239 (MQ=255)
                                                                          tATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTAACTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CACCGCTGGCAACAGCGGCCCACGCGAAGCGGTGCCCGGGACGGCGCACCGGGGTCGTCggaacgggc                                                                                                                                                                >  2:449667/1‑239 (MQ=255)
                                                                                 tCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGTCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACACCGCCCCCCGCCAATGGGTCTTCGGGGGGGCCCCCCCGGGTGGTCttaacgtgctggggta                                                                                                                                                         >  2:640439/1‑235 (MQ=255)
                                                                                    ggTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCCCGAAGGGGTTCTCGGGATGGCGCCCCGGGGGCGTCagaacgggctggtgaaggg                                                                                                                                                      >  2:589735/1‑236 (MQ=255)
                                                                                    ggTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCC‑CCACGCTGGCAACAGCGGCCCCCCCGAAGTGGTTCTCGGGATGGCCCACCGTGGTCGTCtgaaccggctggggaacgg                                                                                                                                                      >  2:718832/1‑238 (MQ=255)
                                                                                     gcccgccgACTTCTAATTGCGAAGAGAAAAAACC‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctggtgaacgtg                                                                                                                                                     <  2:281308/233‑1 (MQ=255)
                                                                                     gTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCttaacgtgctggtgaacgt                                                                                                                                                      >  1:536595/1‑238 (MQ=255)
                                                                                     gTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGTCtgaacgggggggggaacggg                                                                                                                                                     >  2:256946/1‑237 (MQ=255)
                                                                                      ccgcgcgATTTTAAATAGGGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctggtgaacgtg                                                                                                                                                     <  1:256946/237‑1 (MQ=255)
                                                                                      ccgcgccACTTTCAAGACAGAAGA‑AAAAAACGTCTTGTAAAGGACA‑GTGC‑CCGCCCCTGAAGGTCTTGAACTTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCCCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCtgaacgtgctggtgaacgtg                                                                                                                                                     <  2:118559/237‑1 (MQ=255)
                                                                                          gcgACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGCCGTCTGAACGTGCTGGTGAACGTGCTggg                                                                                                                                                >  1:624454/1‑239 (MQ=255)
                                                                                                        ggcaaagaAAAAACC‑CTCTTTAAAGGCACCTGC‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCGCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGc                                                                                                                                     <  2:624454/232‑1 (MQ=255)
                                                                                                        cGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTCATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGCAGTGGGTCCCGGGATGGCGCACCGGGACCGTCTGAACGGGGCGGTGAACGTGCTGGGGAAAAAAcacccaa                                                                                                                                  >  2:583413/1‑233 (MQ=255)
                                                                                                             agaAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTAGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTg                                                                                                                             >  1:280759/1‑239 (MQ=255)
                                                                                                             agaAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGCCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGCCTTg                                                                                                                             >  1:988138/1‑239 (MQ=255)
                                                                                                                          tcttAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGCCTTTTTCGACGAGTTc                                                                                                                 >  1:350950/1‑239 (MQ=255)
                                                                                                                          tcttAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGtt                                                                                                                  >  1:147532/1‑238 (MQ=255)
                                                                                                                               ggccaa‑CTGA‑CCGACGGTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAAGCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCgg                                                                                                            <  1:373574/235‑1 (MQ=255)
                                                                                                                                gCGAT‑CTGC‑CCGCCGCTTAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCGGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAAGCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:922206/239‑1 (MQ=255)
                                                                                                                                 gcgcccTGC‑CCCCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCGGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:691545/234‑1 (MQ=255)
                                                                                                                                  gga‑CTGACCCGCCGCGTAAGGTCCTGAACGTTACCCGGGCGCAAAATTCCCTGGCGCAAAACGCTTCCCG‑GTGGAAGGCGGTGACGGTTTAACCCCGAGGCTTAAAGGAGTGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTa                                                                                                          <  2:743319/237‑1 (MQ=255)
                                                                                                                                  gcgatctccccgccgCTGAAGGTTTTGAACTTTTCCTCGGCGCAAAATTCCCTGGCGCAAAACGCTGCGCG‑CTGGAAGGCGGTAGCGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:815154/230‑1 (MQ=255)
                                                                                                                                   aa‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGCCTGAACGCGCTGGTGAAGGGGCGGGGTAAAAAACCCCAAAACTTGTTCGACGAGTTCGCCGGaaaa                                                                                                        >  2:564889/1‑239 (MQ=255)
                                                                                                                                    aacttgcccgccgCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  2:648413/230‑1 (MQ=255)
                                                                                                                                    aaacttcccggcgCTGAAGGTCTTGAACGTTACCTCGGGCCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGt                                                                                                           <  1:499253/230‑1 (MQ=255)
                                                                                                                                       ccgccccccgcTGAAGGTTTTGAACTTTTCCCCGGGCCAAAATTCCCTGGCCCAAAACGCTTCTCG‑CTGGAAGGGGGTGACGCGTTAATCCCGATGCTTAAGGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACa                                                                                                      <  2:540090/232‑1 (MQ=255)
                                                                                                                                        gA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATa                                                                                                    <  1:452813/239‑1 (MQ=255)
                                                                                                                                         a‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATaa                                                                                                   >  1:22161/1‑239 (MQ=255)
                                                                                                                                              ccgcAGAAGGTTTTAAACGTTCCCTCGGCCAAAAATCCCCTGGCGCAAAGCGCTTCTCG‑CGGGACGGCGGTGACCCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGaa                                                                                               <  2:866956/239‑1 (MQ=255)
                                                                                                                                                 cTGAAGGTTTTGAACGTTACCTCGGAGCAAAATTCCCTGGCGAAAAACGCTTCTCG‑CTGGAAGGGGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAacac                                                                                            <  2:784644/239‑1 (MQ=255)
                                                                                                                                                         cTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTTATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTCAACATAAAGAAACCCTCGGCAc                                                                                    >  1:292156/1‑239 (MQ=255)
                                                                                                                                                         cTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCgg                                                                                                                                                                                                                  <  1:130976/113‑1 (MQ=255)
                                                                                                                                                         cTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCgg                                                                                                                                                                                                                  >  2:130976/1‑113 (MQ=255)
                                                                                                                                                         cTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGCCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGGAAAAATAAAGAACACCCCGGCAc                                                                                    >  2:811251/1‑239 (MQ=255)
                                                                                                                                                             aaCGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGt                                                                                >  1:250758/1‑239 (MQ=255)
                                                                                                                                                                 ttcccTCGGCCCAAAATTCCCTGCGGCAAACCGCTTCCCC‑TTGGAAGGGGGTTACCCGTTAAACCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACg                                                                            <  2:899593/236‑1 (MQ=255)
                                                                                                                                                                  tACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACgg                                                                           >  1:247353/1‑238 (MQ=255)
                                                                                                                                                                  tACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGCCTGAACGTGCTGGGGAACGTGCTGGGGAAAAAACCGCAGGACTTGTTCGCCCGGTTGCCCGGTAAAAATAAAAAAAACCCCGGCCCGGGGGACgg                                                                           >  2:215606/1‑238 (MQ=255)
                                                                                                                                                                  tACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGCGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTAGCCGAGTTCGCCGCTAAACAAAAAAAAAAACCCGCAACGGGGGACGt                                                                           >  2:222175/1‑239 (MQ=255)
                                                                                                                                                                          cgcAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGGTGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGGGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACAAAAGAAAAACCCCCGCACGGGGGagcgcaaaaacc                                                                   >  2:415176/1‑228 (MQ=255)
                                                                                                                                                                            cAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGGGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGGGACGGGAAATACcac                                                                 >  1:668820/1‑239 (MQ=255)
                                                                                                                                                                                    ccTGGCGCAAAACGCTTCCCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCtt                                                         <  1:351662/239‑1 (MQ=255)
                                                                                                                                                                                    ccTGGCGAAAAACGCTTCTCG‑CTGGAAGGGGGGGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCtt                                                         <  1:1071384/239‑1 (MQ=255)
                                                                                                                                                                                           cAAAAC‑CTTTTCGCCTGGAAGGCGGTGCCGCGTGAACCCCGATGCTTAAAGGAGTGACCCG‑CCCCGCTGGCAACCGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCCCCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  2:597367/239‑1 (MQ=255)
                                                                                                                                                                                            aaaaCTCTGCTCG‑CTGGAAGGCGGTGGCGCGTTAATCCCGATGTTTAAAGGGATGATCCG‑CCACGCTGGCAACAGCGGCCCCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  1:243986/238‑1 (MQ=255)
                                                                                                                                                                                            aaaaCGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCAGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAGCATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  1:589735/238‑1 (MQ=255)
                                                                                                                                                                                            aaaaCGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAAGCCCGATGCTTAAAGGGATGACCCG‑CCACGCTTGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCt                                                  <  1:725934/238‑1 (MQ=255)
                                                                                                                                                                                                 ggtgttcG‑CTGGAAGTCGGTGACGGGTGAAGCCGGAGGGTAAGATAGATGCACCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGAGGGCGCACCGGGGACGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTc                                            <  2:775197/234‑1 (MQ=255)
                                                                                                                                                                                                 ccTTCCCT‑CTGGAAGGCGGGGACGCGTTAACCCCGATGTTTAAAGAGATGACCCG‑CCACGCTGGCCAACGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTc                                            <  2:716132/238‑1 (MQ=255)
                                                                                                                                                                                                   ttCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAg                                                                                                                                                                                                       <  1:784124/82‑1 (MQ=255)
                                                                                                                                                                                                   ttCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAg                                                                                                                                                                                                       >  2:784124/1‑82 (MQ=255)
                                                                                                                                                                                                              aaGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATgg                                 <  1:91247/238‑1 (MQ=255)
                                                                                                                                                                                                                gggggTGACGGTTTAACCCCGGTGGTTAAAGGGTTGGCCCG‑CCCCCGTGGCAACAGGGGCACCCGGGAAGTGGTTCTCGGGATGGCGCACCGTGGGCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcg                               <  2:508588/235‑1 (MQ=255)
                                                                                                                                                                                                                ggCGGTGAGGCGTTAATCCCGAGGCTTAAAGAGATGATCCG‑CCACGCGGGCAACAGGGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcg                               <  1:232414/238‑1 (MQ=255)
                                                                                                                                                                                                                   ggTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGGTCGCCGGTAAACATAAAGAACACCTCGCCACGGGGGACGGGAACTCCCACATGGGCTTCTCGTCTGCCTTCCAGACCGAGCGCGGccc                           >  2:824114/1‑238 (MQ=255)
                                                                                                                                                                                                                        ccctttAATCCAGATGGTTAAAGAGGCGATCCC‑CCCGCCTGGCAACAGCGGCCCCCGCGAAGTGGTTCTCGGGATGGCGCACCGGGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTg                       <  2:284387/234‑1 (MQ=255)
                                                                                                                                                                                                                           gTTAATCCCGATGCTTAAAGAGATGAGCCG‑CGCCGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCAcc                   <  1:1015213/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ccgcttcTTAAAAGGGTGATCCGCCCCCGCT‑GCAACCGGCGCCCCCGCTAAGTGTGTTGCGGGAAGGCCCCCCGGGGTCGTCTGAACGGGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct            <  2:362635/233‑1 (MQ=255)
                                                                                                                                                                                                                                  ccGATGCTTAAGGGGAGGAACCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGGTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGCACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct            <  1:640439/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ccGAGGCTTAAAGGGATGACCCG‑CCACGCTGGCAACCGCGGCCCCCGGGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACCCCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACctggcgct            <  1:718832/239‑1 (MQ=255)
                                                                                                                                                                                                                                     aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGc                                                                                                                                     >  2:704858/1‑115 (MQ=255)
                                                                                                                                                                                                                                     aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGc                                                                                                                                     <  1:704858/115‑1 (MQ=255)
                                                                                                                                                                                                                                     aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGACCACCCCGGAACGGGCGACGTGAATTACCCCAGGGGCTTTTCGTCTGACTTCCAAACCGAAGGCGGCCTGGGGCACcgggggctggg         >  2:32156/1‑238 (MQ=255)
                                                                                                                                                                                                                                     aTGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGGGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTCGGGCACctggcgctggc         >  1:926812/1‑239 (MQ=255)
                                                                                                                                                                                                                                       gCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCCCCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATggcg                               <  1:51782/215‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                            
ACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACG‑CTTCTTAAGCGAA‑CTGA‑CCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGCAAAATTCCCTGGCGCAAAACGCTTCTCG‑CTGGAAGGCGGTGACGCGTTAATCCCGATGCTTAAAGAGATGATCCG‑CCACGCTGGCAACAGCGGCACCCGCGAAGTGGTTCTCGGGATGGCGCACCGTGGTCGTCTGAACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAAC  >  NC_000913/759192‑759662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑