Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 762,113 T→C 22.2% S198P (TCC→CCC)  sucB → dihydrolipoyltranssuccinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913762,1130TC22.2% 100.8 / 4.5 57S198P (TCC→CCC) sucBdihydrolipoyltranssuccinase
Reads supporting (aligned to +/- strand):  ref base T (30/13);  new base C (3/11);  total (33/24)
Fisher's exact test for biased strand distribution p-value = 2.07e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTGCTGGCTGAACACAATCTCGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCGGT  >  NC_000913/761879‑762339
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  
cGCCTGCTGGCTGAACACAATCTCGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCa                                                                                                                                                                                                                                 >  1:255286/1‑238 (MQ=255)
     gctggctgAACACAATCTCGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGGGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAAAGGGGCTGCTGGAAGCGAAAAACCCCACCGCCa                                                                                                                                                                                                                           >  1:791606/1‑239 (MQ=255)
                    tctcGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAACGCGTCGGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCa                                                                                                                                                                                                            >  1:785036/1‑239 (MQ=255)
                        gagccgggcgcCATGAAAGGCAGGGGGGGGGGTGGTCGGCGGACGGGTAAGGTGGGGGAAAAACGTGGGGGGAAAGCCCCGGGGAAAAGGTCTGCTCGGGTGCGGGCTGCCCCGGCGGCGCAACGGGCGCTGGCTGCAAGTAGTAAAAAACGGGTCCGGTGGACGCCCCTGCGTAAGCGGGTGGCAGAGAGTCTGCGGGAAGGAAAAACCTCCACCGCCATGCTGACCACGTTCAACGa                                                                                                                                                                                                        <  2:578277/232‑1 (MQ=255)
                              aGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTAAACGAAGTCaa                                                                                                                                                                                                  >  1:663723/1‑239 (MQ=255)
                                     ttAAAGGCCCGGGGGGGGGGGGGGGTCTGACTCGGGAAGGTGTGGAAAACCAGCTGGGGAAACCCCTGGGGAAAGGGGCTGCTCCGGCAGGGGCTGCCCGGGGGGGCAAACCGGCTCTGGCTGACGGGAGGGAAAAAGGTGTCCCGATGACTCGCCTGGTTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAg                                                                                                                                                                                           <  2:279600/239‑1 (MQ=255)
                                             aCCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCCCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGGAAGCGTGGGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCGGACCACGTTCAACGAAGTCAAAAAAAAGCCGAtttg                                                                                                                                                                                   >  2:1030460/1‑237 (MQ=255)
                                               cGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCCGCGTACACGGGTGGCAGAGCGTCCGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCAACTCTAACAAAGTCAACAAAAAACCGATTAAgg                                                                                                                                                                                 >  2:186625/1‑239 (MQ=255)
                                                ggTGTGGGGGGTCGTTTGACCCGGGAAGATGGGGAAAAACATCGGGCGAAAGCCCGGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGa                                                                                                                                                                                <  1:557240/239‑1 (MQ=255)
                                                    tgggtggTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGGGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAAAAGGAAGCCGATTTAGGAACTg                                                                                                                                                                            >  2:672196/1‑239 (MQ=255)
                                                    tgggtggTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGCGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGCCCACGTTCAACGAAGTCAACATGAAGCCGATTATGGAttt                                                                                                                                                                             >  1:892543/1‑236 (MQ=255)
                                                           tcgtcTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCCGAGGGTTCGCTGGAAGCGCAAAACTCCACCGCCATCCTGACCACGTTCAACGAAGTCAACATGAACCCCACTTTGGCTCTCCGaaaac                                                                                                                                                                     >  2:987396/1‑237 (MQ=255)
                                                           ccgtcTGACTCGTGAAGATGTGGAAAACCATCGGGCGAAAGCCCCGGCGAAAGAGTCTGCCCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAg                                                                                                                                                                      <  1:591202/237‑1 (MQ=255)
                                                            cgtcTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGCCCACGTTCAACGCAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCa                                                                                                                                                                    >  1:919220/1‑239 (MQ=255)
                                                            cgtcTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGGGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCCCCGCCATGCTGCCCCCGTTCACAGAAGTCAACATGAAACCGATAATGGAATTCGGTAAGCa                                                                                                                                                                    >  2:840842/1‑239 (MQ=255)
                                                                       tGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTTCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTAAACGAAGTCACAATGAAGCCGATTAAGGATCTGCGTAAACAGAAAGGGGAAg                                                                                                                                                         >  2:65344/1‑239 (MQ=255)
                                                                              gtgGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGAc                                                                                                                                                                                                                    >  2:336693/1‑173 (MQ=255)
                                                                              ggggAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGAc                                                                                                                                                                                                                    <  1:336693/171‑1 (MQ=255)
                                                                                      acctCTGGGGAAAACCCCGGGGAAAAGGGTCTCCCCGGGCAGGGGCTGCCCGGGGGGCGCAACCGGCTTTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGGGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGc                                                                                                                                           <  2:255286/236‑1 (MQ=255)
                                                                                       cctggggagAAACCCCGGGGGAAGGGGCCTGCCCGGGCGGGGGTGGCGCCGGGGGCCAACCCGGCTGTGGTGGCAGGGAGGGAAAACCGTGTGCCGATGACCCCCCTGCGAAGGGGGGGGGCAGAGTGTCTGCGGGAAGGGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCa                                                                                                                                         <  2:148130/231‑1 (MQ=255)
                                                                                            ggCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGGGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACCTGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGCAAAAAGCCACGGc                                                                                                                                     >  1:643033/1‑238 (MQ=255)
                                                                                                  aGCCCCGGCGAAAGCGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTACGCGTGGGGCAGAGCGCCTGCTGGAAGGGAAAAACTCCCCCCCCATGCTGGCCCCGTTCACCGAAGTCAACATAAAGCCGACTATGGACTTGCGTACACAGAACGGTAAAACGTTTGAAAAACCCCAAGGCATCCGTc                                                                                                                              >  2:62515/1‑239 (MQ=255)
                                                                                                       cGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCCCCGCCATGCTGACCCCGTTCAACGAAGCCAACATGAAGCCGATTAAGGATCTGCGTAAGCAATACGGGAAAGCGTTTGAAAAAACCCACGGACACCGCCTgggg                                                                                                                         >  2:482241/1‑238 (MQ=255)
                                                                                                             aagtggtttGCCCCGGGAGGGGTTGCTCGGGGGGCGCACCCGGGTCGGGCGGCGCGTGGTGAAAACCGGGTCCGGATGACTCGCCTGCGTAAGGGTGGGGCAGGTCGTCTGCTGGAAGCAAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTAt                                                                                                                    <  2:1044712/231‑1 (MQ=255)
                                                                                                               tggtgttGCGCCGGCGGGGGGTCCGCCGGGTGCGCACCCGGGTCTGGCGACAGGGTGGGAAAAACGTGTCCGGATAACTCGCCTGCGTAGGCTTGGGGCAGGGCTTCGGGTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGt                                                                                                                  <  2:405339/233‑1 (MQ=255)
                                                                                                                     tGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACCTGAAGCCGATTATGGTTCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCCCGGCAACCGCCTGGGCTTTATGTCCTTc                                                                                                             >  1:899380/1‑238 (MQ=255)
                                                                                                                            gCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGt                                                                                                                               >  1:13009/1‑213 (MQ=255)
                                                                                                                                 ggCTGCTCCGGGGGCGCACCCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCTTGCGTAAGCGTGTGGCAGAGCTTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCg                                                                                                 <  1:632676/238‑1 (MQ=255)
                                                                                                                                           gcggcgCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGACAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAACGTTTGAAAAACGCCCCGCCATCCGTCCGGGGTTCAAGCCCTTCAACCGGGAAGAGGGGGTTGGAg                                                                                       >  2:575825/1‑238 (MQ=255)
                                                                                                                                                 cAACCGGCTCTGGCGGCCCGTAGTGAAAAACGTTTCCCGATGTCCCGCCTGCGTAAGCGTTTGGCAGGGCGTCTGCTGGAAGCGAAAAACCCCCCCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGa                                                                                 <  1:220150/238‑1 (MQ=255)
                                                                                                                                                     ccGGCTTTGGCTGCACGTAGTGAAAAACGTGTCCAGATGACTCGCCTGCGTAAGCGTGGGGCAGAGCGTCGGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACg                                                                             <  1:65344/239‑1 (MQ=255)
                                                                                                                                                          gctggcgtgccGTAGTGAAAAACGTGTCCCGAGGCCCCGCCTGCGTAGGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTAcc                                                                        <  1:305315/229‑1 (MQ=255)
                                                                                                                                                           cgtgCTCCACCTGGTGAAAAACGTGTCTCCGTGACTCCGCCGCCGTAGGCGGGGTGAAGTGCTCTTGCTGAAACCAAAAACCCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTAcc                                                                        <  2:31711/236‑1 (MQ=255)
                                                                                                                                                                ggCACGTATGAAAAAGCGTGCCCCGAGGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGaa                                                                   <  1:184709/237‑1 (MQ=255)
                                                                                                                                                                   aCGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCCTCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGGGGTTGAAGCCCCGAAACGTTACCCGGaaagga                                                               >  2:66233/1‑235 (MQ=255)
                                                                                                                                                                    acggggaaaaagCTTGTCCCGTTGTCTCGCCTGTGTACGCGGGGGGACGGGCGTCTGCTTGAACCAAAAACCCCGCCCGCCATGCTGACCACGTTCAACGAAGGCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGaa                                                              <  2:338410/232‑1 (MQ=255)
                                                                                                                                                                      tAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCATCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAACCGTTACCCGCAAGTGAAc                                                             >  1:58803/1‑238 (MQ=255)
                                                                                                                                                                       gagaaaaacCGGGTCCCGATGCCTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGaa                                                              <  1:672196/232‑1 (MQ=255)
                                                                                                                                                                        gTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGGGGTTGAAGCCCGGAAACGTTACCCGGAAGTGAACg                                                            >  2:532025/1‑237 (MQ=255)
                                                                                                                                                                                 cGTGCCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACCCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGa                                                  <  1:972189/239‑1 (MQ=255)
                                                                                                                                                                                    gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGaa                                                              >  1:302645/1‑224 (MQ=255)
                                                                                                                                                                                    gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGGTTGTATc                                                    >  1:338410/1‑234 (MQ=255)
                                                                                                                                                                                    gtCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGGGGTTGAAGCCCGGAAACGTTACCCGGAAGGGAACCCTCCTATCGACgg                                               >  2:131859/1‑239 (MQ=255)
                                                                                                                                                                                            gACTCGCCGGGGGAAGCGTGTGGAAGGCGTTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGAACACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACg                                       <  1:1005395/239‑1 (MQ=255)
                                                                                                                                                                                             aCTCCCCTGGATAAGGGTGGGGCAGGGGGTGTGGTGGAAGCGAAAACCTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACg                                       <  1:131859/238‑1 (MQ=255)
                                                                                                                                                                                               tCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGGGGTTGAACCCCTGAAACGTTACCCGAGAGGGAACGCTTCTATCGAGGGcctggccctta                                    >  2:482947/1‑229 (MQ=255)
                                                                                                                                                                                               tCGCCAGCGGAGGGGTGTGGCGGGCGTTCTGCTGGAAGCAAAAACCCCCACCGCCATGCTGACCAAGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTgg                                    <  1:62515/239‑1 (MQ=255)
                                                                                                                                                                                                  gCCTGCGAAAGCGTGGGGGAGGGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGtt                                  <  1:232959/239‑1 (MQ=255)
                                                                                                                                                                                                                 tgGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCttt                                                                                                                      >  2:123041/1‑140 (MQ=255)
                                                                                                                                                                                                                 tgGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCttt                                                                                                                      <  1:123041/140‑1 (MQ=255)
                                                                                                                                                                                                                 tgGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATTACGTGGTTTACCACAACTATTTc                   >  1:148481/1‑239 (MQ=255)
                                                                                                                                                                                                                 tgGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTc                   >  1:178470/1‑239 (MQ=255)
                                                                                                                                                                                                                 tgGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGGGAAAGCGGGGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGGCGGGGGGTACGGGGTTTACACAAACAATTTc                   >  2:874959/1‑239 (MQ=255)
                                                                                                                                                                                                                       agCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACTTGGTTTACCCCAACTATTTCGACGTc             >  1:715360/1‑239 (MQ=255)
                                                                                                                                                                                                                            tCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGGGGTTTACCACAACTATTTCGACGTCAGCa         >  1:631313/1‑239 (MQ=255)
                                                                                                                                                                                                                              tgtaggAAGCAAAACACCCCACCGCCATGCTGACCACGTTAAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATg       <  1:790920/235‑1 (MQ=255)
                                                                                                                                                                                                                                   gAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCggg  >  1:214603/1‑238 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                  
CGCCTGCTGGCTGAACACAATCTCGACGCCAGCGCCATTAAAGGCACCGGTGTGGGTGGTCGTCTGACTCGTGAAGATGTGGAAAAACATCTGGCGAAAGCCCCGGCGAAAGAGTCTGCTCCGGCAGCGGCTGCTCCGGCGGCGCAACCGGCTCTGGCTGCACGTAGTGAAAAACGTGTCCCGATGACTCGCCTGCGTAAGCGTGTGGCAGAGCGTCTGCTGGAAGCGAAAAACTCCACCGCCATGCTGACCACGTTCAACGAAGTCAACATGAAGCCGATTATGGATCTGCGTAAGCAGTACGGTGAAGCGTTTGAAAAACGCCACGGCATCCGTCTGGGCTTTATGTCCTTCTACGTGAAAGCGGTGGTTGAAGCCCTGAAACGTTACCCGGAAGTGAACGCTTCTATCGACGGCGATGACGTGGTTTACCACAACTATTTCGACGTCAGCATGGCGGT  >  NC_000913/761879‑762339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: