Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,274,349 T→G 26.1% G57G (GGT→GGG yejB → microcin C ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,274,3490TG26.1% 122.7 / 14.4 75G57G (GGT→GGGyejBmicrocin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (15/38);  new base G (20/2);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 5.31e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTCC  >  NC_000913/2274115‑2274586
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
acccTGGGGGTAGGACGTCAATAAAGCGGCCAACCGGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGtt                                                                                                                                                                                                                                             <  1:643300/236‑1 (MQ=255)
    tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGATTACCCGGCGCAGGCGGTGAAGGGGtttggtc                                                                                                                                                                                                                                        >  1:535882/1‑234 (MQ=255)
      gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGGAAGGGTTTCGTgcc                                                                                                                                                                                                                                       >  1:934641/1‑238 (MQ=255)
      gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGTTTACCCGGCGCAGCCGGGGAAAGGGTTCGGgcga                                                                                                                                                                                                                                      >  2:261104/1‑237 (MQ=255)
          tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGCCCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCCCGGGGGGGGAAGGGGTTCGGggcagccc                                                                                                                                                                                                                                  >  2:284657/1‑238 (MQ=255)
          tATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGGATTACCCGGCGCAGGCGGGGAAGGGTGTCGGgccaccca                                                                                                                                                                                                                                  >  1:319779/1‑239 (MQ=255)
            tGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTAAGTTTGGGATTGCCGGGGAATTACCCGGCGCAGGCGGTGAAGGGGTTCGGgcccgccagg                                                                                                                                                                                                                                >  2:570086/1‑237 (MQ=255)
                gTCAATAAAGCTGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGCAATGCCGGGGTATTACCCGGCGCAGGCGGTGAAGGGTGTCGTgcccggcacgacca                                                                                                                                                                                                                            >  1:480170/1‑228 (MQ=255)
                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGTTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAAGGCCGGAGAATTACCCGGGGCAGGCGGTGAAGGGGTGCGTGCCAGCCATGCGCa                                                                                                                                                                                                                            >  1:118184/1‑239 (MQ=255)
                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGCGTATTACCCGGCGCAGGCGGGGAAGGGGTTTGGGCCCGCCATggcca                                                                                                                                                                                                                            >  1:369472/1‑235 (MQ=255)
                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCa                                                                                                                                                                                                                            >  1:190821/1‑239 (MQ=255)
                    aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCCCAGGCGGTGAAGGTGTTCGGGCCAGCCATGCGCaaaag                                                                                                                                                                                                                        >  1:683531/1‑237 (MQ=255)
                            gCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCCGGACCAAACGGgggtat                                                                                                                                                                                                                 >  1:719339/1‑236 (MQ=255)
                            gCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGCCGCAGGCGGGGAAGGGGTTCGTTCCAGCCATGACCAAACGGGTGTCg                                                                                                                                                                                                                 >  1:512385/1‑238 (MQ=255)
                             ccAAACTACCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGAggcggc                                                                                                                                                                                                               >  1:337722/1‑239 (MQ=255)
                                      cGTCCGCCAGCAAACGGGGGGAGTAGTTGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACTTTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAg                                                                                                                                                                                                       <  2:512385/238‑1 (MQ=255)
                                         cccccggCAAAGGGGGAGTGGAGATGGGCGCTTACCTGATTCGCGGTCTGGTGCGGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTGCATCGTGCAAATGGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                                   <  2:727503/233‑1 (MQ=255)
                                         ccGCCAGCAAACAGGGGGGTGAGATGGGCGTTTACCTGATTCGCCTTCTGTTTCTGGGCGTCCCAACCTTATGGGCGATTATCCCCATCAACTTTTTCATCGTGCAAATTGCGCCTGGGGGTCCGGTCGACCAGGCCACCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                                   <  2:49215/239‑1 (MQ=255)
                                         ccGCCAGCAAACAGGGAAAGTAGAGTGGCTCTTACCTGATTCGCCGTCTTTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    <  1:78684/238‑1 (MQ=255)
                                                       ggAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCCGGCGGTGAAGGGGTTCGGTCCAGCCATGCGCCAACGGGGGGTGGCAAAATCACGGACAGGAATTTCCGTgg                                                                                                                                                                                     >  1:1011819/1‑239 (MQ=255)
                                                         agagTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCGCAGGCGGGGAAGGGGTTCTGGCCAGCCCTGCGCAAAAGGGGGTGGGCCATATCAGGGACAGTAATTTCCATGGCg                                                                                                                                                                                   >  1:70584/1‑239 (MQ=255)
                                                          gagTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGGGTCGGCAATTTCAGCGCCAGTAATTACCATGGCgg                                                                                                                                                                                  >  1:640738/1‑239 (MQ=255)
                                                              ggATGGGGGCTTCCCTTATCCCCCGTCTGTTGTGTGTGATCCCAACATTGGGGGCGATTACCCCCATCAACTTTTTCATCGTGCAAATGGCGCCTGGCGGTCCGGTCGCCCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                               <  2:735191/237‑1 (MQ=255)
                                                              atgtGGGCTTTTACCTGATTCGCCTTCTGTTGCTGGGTACCCCAAAATTAGGGGCGATTTCCACCATCAACTTTTTCCTCGTGCAAATTGCGCCTGGGGGGCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                               <  2:169632/235‑1 (MQ=255)
                                                               gATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGCGTATTACCCGGCGCAGGCGGGGAAGGGGTTCGGGGCCGCCCTGCGCAAACGGGGGTCGGCAATATCAGGGACGGGAATTACCCTGGCGGAagcg                                                                                                                                                                             >  1:488522/1‑239 (MQ=255)
                                                               gATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCCTGGGCAAACGGGGGTCGGCAAAATAAGCGACAGTAATTACCGgggggggacgg                                                                                                                                                                             >  2:927980/1‑234 (MQ=255)
                                                                 tGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGGAGGTGTTCGTGCCAGCCATGGGCAACCGGGTGGCGGCAATATTAGCGCCAGGAATTTCCGGCGCGGAcggcgg                                                                                                                                                                           >  1:400046/1‑235 (MQ=255)
                                                                   gggctttACCTGTTTCGCCTTTTGTTGCTGGTGACCCCAACATTAGGGGCGATTACCCCCATCAACTTTTTCAGCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                         <  2:934641/234‑1 (MQ=255)
                                                                    gcccTTACCGGATTCCGCGTCTGGTGGTGGGGATCCCAACATTATGGGCGATTATCACCAGCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                         <  1:992711/235‑1 (MQ=255)
                                                                     cgcTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACCGGTGTCGGGAATATACACGACAGGAATTACCGTGGGGGGCCCgggatag                                                                                                                                                                       >  2:49567/1‑234 (MQ=255)
                                                                       cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGGCAGTAATTACCGGGGGGGACGCGGGTTTGa                                                                                                                                                                      >  1:735191/1‑238 (MQ=255)
                                                                                 tCGCCTTCTGTTGCGGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGa                                                                                                                                                           <  1:284657/239‑1 (MQ=255)
                                                                                   cgggtttgttgttgtGGATCCCAACATTTGGGGGGTTTTACCCCACAACTTTTTTCACGGGGAAATTTGCGCCGGGGGGTCCGGTCGCCCGGGCCAGCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATc                                                                                                                                                         <  2:1064000/233‑1 (MQ=255)
                                                                                      ttttttttcTGTTGATCCCAAAATTAGGGGCGATTACCCCCATCAACTTTTTCATCGTGCAAATTGGGCCTGGCGGCCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCt                                                                                                                                                      <  2:190821/231‑1 (MQ=255)
                                                                                      gttgtgttttGGTGATCCCAACTTTATGGGCGATTATCCCCATCACCTTTGTCATCGTGAAAATTGCGCCTGGGGGTCCGGTCGGCCCGGCCAGCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCt                                                                                                                                                      <  2:480170/230‑1 (MQ=255)
                                                                                                 gTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTAACGTGGCGGACGCGGGTTCGAGTCAGAAGGGATcccgtggataa                                                                                                                                               >  1:1064000/1‑225 (MQ=255)
                                                                                                      ccgaaCATTATGGGCGATTATCTCCATCAACTTTTTCTTCGTGCAAATTGCGCCTGGCGGTCCGGCCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAACGTGTTCGTGCCATCCATGCGCAAACGGGTGTCGGCAATATCAg                                                                                                                                                                                                       <  1:359259/171‑1 (MQ=255)
                                                                                                       ccAACATTATGGGCGTATATCCCCATCAACTTTTTCCTCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTa                                                                                                                                     <  1:105652/239‑1 (MQ=255)
                                                                                                                tGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATAAGCGCCAGTAATTACCGGGGCGGACCCGGGTTAGATCCAGAAGGGATCGGTTGGATCCACCAACGCTACGGGTTTgg                                                                                                                            >  1:1023512/1‑238 (MQ=255)
                                                                                                                     tcttttccccATAAATTTTTTCATCTGGAAAATGGGGCCGGGCGGTCCGTGCGCCCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAg                                                                                                                        <  2:932694/230‑1 (MQ=255)
                                                                                                                     gATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCg                                                                                                                                                                                                     >  1:197081/1‑161 (MQ=255)
                                                                                                                     gATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCg                                                                                                                                                                                                     <  2:197081/161‑1 (MQ=255)
                                                                                                                                    cTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    <  1:465087/147‑1 (MQ=255)
                                                                                                                                      ttttcgagcGTGCAATTGGGGCGTGGCGGTCCGGGCGACCCGGCCATGGCCGACTTTGAGTTTGGTAATGCCGGAGTATTACCCGGCCCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTa                                                                                                      <  2:10668/231‑1 (MQ=255)
                                                                                                                                               gTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAGATCCAGAAGGGATCGCTGAGATCCCTCATCGCTACCGGTTTTATAAGCCGATCCACGAACGTTACTTCaaaa                                                                                              >  1:847846/1‑238 (MQ=255)
                                                                                                                                                tGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCCAACGGGTGTCGGCAATATCAGCGGCCGTAATTAACGGGGCGGGCGGCGGTTAGATCCAGAAGGGATACCTGTGATACCCCCTCCCTACGGGTTTTATAAACCGCATCCCGCACGTTTCTTCaaaaa                                                                                             >  2:245730/1‑237 (MQ=255)
                                                                                                                                                    aaTTGCGGCTGGGGTTCCGTTCGACCGGGTACGCGCCGCCATGGAGTTTGGTAATGCCGGAGGATTGCCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                                        <  2:734814/239‑1 (MQ=255)
                                                                                                                                                    aaTTGCGCCTGGCGGGCCGGTCGACCAGGCATTCCGCGGCATTGAGTTGGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTTAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctc                                                                                         <  1:898633/238‑1 (MQ=255)
                                                                                                                                                    aaTTCCGCCTGGCGGTCCGGTTGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGAAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                                        <  1:808671/239‑1 (MQ=255)
                                                                                                                                                    aaTGGCGCCTGGCGGTCCGGGCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGctct                                                                                        <  1:334780/239‑1 (MQ=255)
                                                                                                                                                                cggtccggtcGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGATTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATc                                                                             <  1:880054/238‑1 (MQ=255)
                                                                                                                                                                                tccATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCg                                                             <  1:458332/237‑1 (MQ=255)
                                                                                                                                                                                  cttccccgccATGGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGGGGTTAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACCATAATTACCGTTGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTGGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATa                                                          <  2:1009381/234‑1 (MQ=255)
                                                                                                                                                                                  aagcgccgcCATTGAGTTTGGTAATGCCGGATTATGACCCGGCGCAGGCGGTGAAGGTGTTCTTGCCCGCCATGCGCAAACGGGTTGCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTGTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATa                                                          <  1:902679/236‑1 (MQ=255)
                                                                                                                                                                                          ccATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAGACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAAAAGTGATCGCTGAGATCACTCATCGCTACGGGTTTGTTAAGCCGATCCACGAACGGTACTTCCAAATGCTCTGGGACTCCATCCCCTTTGATTTTGGCGATAGCCTGtt                                                   >  2:421883/1‑238 (MQ=255)
                                                                                                                                                                                              tGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCCAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCCCa                                              >  1:921623/1‑239 (MQ=255)
                                                                                                                                                                                              gtggTTTTGTAATGGCGGAGTATTTCCCGGCGCAGGCGGTGAAGGTGTGCGGTCCCGCCATGCGCAAAAGGGTGACGGCAATATCAGGGACAGTAATTACCGTGGCGGACGCGGTTTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCa                                              <  2:719339/236‑1 (MQ=255)
                                                                                                                                                                                               gggTTTGGTAATGCCGGAGTTTTACCCGGCGCGGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAg                                             <  2:488522/237‑1 (MQ=255)
                                                                                                                                                                                               gAGTTTGGTTATGCCGGGGTATTACCCGGCGCGGGCGGTTAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATAGCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAg                                             <  2:319779/239‑1 (MQ=255)
                                                                                                                                                                                                    tGGTAATGGCGGAGTATTTCCCGGGGGAGGCGGTTAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGGAATATCAGCGACAGTAATTGCCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                                        <  1:582097/239‑1 (MQ=255)
                                                                                                                                                                                                               gggttttACCCGGGGCCGGGGGTGAAGGTGTGCGTGCCCGCCGTGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACg                             <  2:650862/234‑1 (MQ=255)
                                                                                                                                                                                                               gAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACCGTAATTACCGTGGCGGACGCGGGTTTGGTCCAGAAGGGATCGCTGGGATCACTCATCGCTACGGGTTTGATAAGCCGATCCACGAACCGTACCTCAAAATGCTCTGGGGCTAAATCCGCCTTTGTTTTGGCGCTGACCTGTTTTCCAGGGCCCCGGGGCTggc                              >  2:135032/1‑236 (MQ=255)
                                                                                                                                                                                                                aGTATTCCCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACg                                                                                                             <  1:611198/158‑1 (MQ=255)
                                                                                                                                                                                                                aGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAACGACACGGTGCTGAc                              >  1:373009/1‑237 (MQ=255)
                                                                                                                                                                                                                 gttttccccGGGGCGGGGGGTGAAGGTGTTCGGGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                            <  2:746617/232‑1 (MQ=255)
                                                                                                                                                                                                                 gTATTTCCCGGCGCAGGCGGTGAAGGTGTTCGGGCCAGGCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                            <  1:570086/238‑1 (MQ=255)
                                                                                                                                                                                                                 gTATTACCCGGCGCCGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                            <  1:809064/238‑1 (MQ=255)
                                                                                                                                                                                                                     tACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    >  2:471596/1‑67 (MQ=255)
                                                                                                                                                                                                                     tACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                                    <  1:471596/67‑1 (MQ=255)
                                                                                                                                                                                                                            cgcAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGATCCAGAAGGGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGGGATAGCCCGTTTTGCAGGGCCCCGGGGCGGACGCTGATTTAAGACa                >  1:144607/1‑239 (MQ=255)
                                                                                                                                                                                                                            cgcAGGCGGTGAAGGGGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACCCGGGTTAGATCCAGAAGTGATCCCTGAGATCACCCATCGCTACGGGTTTGATAAGCCGATCCACCAACGTTACTTCAAAATGCCCTTGGACTAACTCCGCTTTGTTTTTTGCGGAAGCCCGTTTTCCACGGCCCCGGTGCTGACGCCGTTTaaaaaaa                >  2:72009/1‑235 (MQ=255)
                                                                                                                                                                                                                            cgcAGGCGGAGAAGGGGGTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGCCGCGGATTAGATCCAGAAGTGATCGCTGAGATTCATCAACGCTACGGTTTTGAAAAACCGAATCACGAACCGTACTTCAAAAAGCGTTTGGACTACACCCGCCTTGATTTTTGTGATAAACCGTTTCTCGCGGCCCCCGTGCTGAGGCTGATTAAAAACa                >  2:834264/1‑239 (MQ=255)
                                                                                                                                                                                                                                  cGGGGAAGGTGTTCGTGCCAGCCCTGCCCAAACGGATTTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGAGCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTg           <  2:369472/238‑1 (MQ=255)
                                                                                                                                                                                                                                   ggTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGc          <  1:730400/238‑1 (MQ=255)
                                                                                                                                                                                                                                     tGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCg        <  1:772572/238‑1 (MQ=255)
                                                                                                                                                                                                                                           tgtTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTcc  >  1:85701/1‑238 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTGAAGGTGTTCGTGCCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGATCCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGCCGGTTTCC  >  NC_000913/2274115‑2274586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: