Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,064,797	T→A	13.5%	intergenic (+19/‑5)	scpB → / → scpC	methylmalonyl‑CoA decarboxylase, biotin‑independent/propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,064,797	0	T	A	13.5%	253.2 / 3.7	94	intergenic (+19/‑5)	scpB/scpC	methylmalonyl‑CoA decarboxylase, biotin‑independent/propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base T (63/16);  new base A (3/11);  total (67/27)
Fisher's exact test for biased strand distribution p-value = 4.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGCCGAT  >  NC_000913/3064560‑3065032                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            ttCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTa                                                                                                                                                                                                                                            <  1:176475/239‑1 (MQ=255) ttCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTAATTAA‑TCCCTGCGAACAAAGGAGTa                                                                                                                                                                                                                                            >  1:574636/1‑239 (MQ=255)        taaaaaGGGCGCAACACCTCGTTGAGAAAGCGCCGTTAGCCATTGCCGTTAGCAAAGAAGAGCTGCGTGTACTGGGCGAAGCAAACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATgg                                                                                                                                                                                                                                     <  1:990967/236‑1 (MQ=255)          cAAATGGCGCACCACATCTGTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGaa                                                                                                                                                                                                                                   <  2:760643/239‑1 (MQ=255)               tgcgcACCCATTCTCTGGGAAAGGGCCGTTACACATGGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACT‑C‑CATGAACGCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCa                                                                                                                                                                                                                              <  2:326282/238‑1 (MQ=255)               ggCGCGCCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCTTTACAAAAGAAGAGCTGCCTGTACTGGTCGAAGCACACC‑C‑CATGAACGCCGATGAATTGGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCa                                                                                                                                                                                                                              <  1:501213/239‑1 (MQ=255)                gcgcACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGAAGAAAAAATGGAAACTCAg                                                                                                                                                                                                                             >  1:38706/1‑239 (MQ=255)                     ccacATCTCTGAAAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGAc                                                                                                                                                                                                                        <  1:482948/239‑1 (MQ=255)                            tctGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGTGCTAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCC‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGACCGCTTTCCTCGAAAAACGTAAACATAA‑TTTGGTTGGTCATTAA‑TCCCTTGTAACGAAGCAGAAAAAATGAAAACTCAGTGGAAAAgagtg                                                                                                                                                                                                                 >  2:530846/1‑235 (MQ=255)                                agaAATCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAAAGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTACGAACGAAGGAGGAAAAATGGAAACTCAGTGGCAAAGGGTGAcc                                                                                                                                                                                                              >  1:570207/1‑238 (MQ=255)                                agaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGACCGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATGAA‑TCCCTGCGAACGAAGGGGTAAAAATGGAAACTCAGTGGACAAGGATGAcc                                                                                                                                                                                                              >  1:81073/1‑238 (MQ=255)                                agaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGGGAACGAAGGGGTAAAAATGGAAACTCAGGGGAAAAGGATGAcc                                                                                                                                                                                                              >  2:78005/1‑238 (MQ=255)                                agaAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGCGTAAAAATGGAAACTCAGTGGCCAAGGATGAcc                                                                                                                                                                                                              >  1:976221/1‑238 (MQ=255)                                        ggttttcccgttGCCGTTATAAAAGGAGAGCTGGTTGCACGGGGCAACGCA‑ACACC‑CGTGACCCCCAAGGAATTTAAGCGTATT‑AGGGGTAGCGCCCGCGGGGTTTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCCAGAAAAACGTAAACCTAA‑TTTCGTGGGTAATTAA‑TCCCTGCGAACGAAGGAGAAAAAATGGAAACCCAGTGAAAAAGGATGACCGCCAATGaa                                                                                                                                                                                                     <  2:371728/229‑1 (MQ=255)                                         cGTTAGCCATTGCCGTTAACAAAAAAGAGCTGCGTGGCGTGGGCAAAGCACACC‑C‑AATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑CCCCTGCGAACGAAGGAGTAAAAATGGAACCTCAGTGGACAAGGATGACCGCCAATGAAg                                                                                                                                                                                                    <  1:564569/239‑1 (MQ=255)                                                   tGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGCCCGCCAATGAACCGGAAAAAAt                                                                                                                                                                                          >  1:409160/1‑239 (MQ=255)                                                   tGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAAt                                                                                                                                                                                          >  1:708838/1‑239 (MQ=255)                                                   tGCCGTTATCAAAGAAGAGCTGCGTGTACTAGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCCCGGTGTATGACAGCGAAGATTACAAGGAAGGGATGAACGCTTTCCTCGAAACACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGCGAAAAAATGGAAACTCAGTGGCCAAGGATGACCGCCAATGAAGCGGAAGAAAt                                                                                                                                                                                          >  1:164307/1‑239 (MQ=255)                                                       tttacaaaaGAAGAGGTGCTTTGACTGGCCGAACCCCACC‑C‑ATTAAACTCGGGTGAATTGAAACTTATGAGGGGGACGCG‑CCGCGCGGTATATAACAGCAAATATTACCAGGAAGTTATGACCGCTTTCCTCAAAAAACGAAACCCTAA‑TTTCTTTGGTCATTAA‑TCCCTGCGAACAAAGGAGTAAAAATGGAACCTCAGTGGACAAGGAGGACCGCCAATGAAGCGGCAGAAATTATc                                                                                                                                                                                      <  2:526251/233‑1 (MQ=255)                                                       tttaaaaaaTAAGGGGTGTGTGTATTGGGCGAACCAACCC‑C‑CAGGACCACCAATGAATTTGAACGTATTCAGGGGGTGCG‑CGGGGGGGTGAATGAGCGCGAAGATTACCAGGAAGGGATGACCGCTTTCCCCGAAAAACGTAACCCTAA‑TGACGTTGTTCATTAA‑CCCCTGCAAACGAGGGAGAAAAAATGGAAACGCAGTGGACAAGAATGACCGCCAATGAAGCGGCAGAAATTATc                                                                                                                                                                                      <  2:574636/233‑1 (MQ=255)                                                       gtaaaaaaaGAAGGGCTGCGTGACCTGGGGGAAGCAC‑CA‑CACAGTAACCCCAATGAATTTGAACGTATCCGGGGGATGCG‑CCGCGGGGTGTATGACAGCAAAGATTACCAGGAAGGGATGAACGCTTTCCGCAAAAACCGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCAAACGAAGGAGTAAAAATGGAAACTCAGTGGAAAAGGATGACCGCCAATGAAGCGGCAGAAATTATc                                                                                                                                                                                      <  1:209771/233‑1 (MQ=255)                                                       gTTATCAAAGAAGAGCGGCGTGTACGGGGCGAACCACACC‑C‑CAAGAACCCAGATAAATTTGAACGTATTCAGGGGATGCG‑CCGCGGGGTGTAAGACAGGGAAGATTCCAAGGAAGGGAGAAACGCTTTCCTAAAAAAACGTAAGCCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGAAAAAATGGAAACTAAGTGGAAAAGGATGACCGCCAATGAAGCGGCAGAAATTATc                                                                                                                                                                                      <  1:479846/239‑1 (MQ=255)                                                                         cGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACAtggt                                                                                                                                                                     >  1:579538/1‑238 (MQ=255)                                                                           tttACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCa                                                                                                                                                                                                                              <  2:285784/177‑1 (MQ=255)                                                                           tgtACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCa                                                                                                                                                                                                                              >  1:285784/1‑179 (MQ=255)                                                                           tgtACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAACGGCAGAAATTAACCACAAAAACCACATGGGGGc                                                                                                                                                                  >  2:153428/1‑239 (MQ=255)                                                                             tACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCAt                                                                                                                                                                >  1:591176/1‑239 (MQ=255)                                                                                 ggcgagaccccacc‑c‑CATGACCCCGGATGAATTAGAACGTGTCGGGGGGACCGC‑CCGGGTGGTGTAAAGCAGCAAATATTCCCAAGAAGGGATGAGCTCTTTCCTAGAAAAACAAAAACATAT‑TGCCGTGTTTCATTAA‑CCCCTGCGAACAAAGAAGTAAAAAGGGAAACTCAGTAGACAGGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAg                                                                                                                                                            <  2:888074/229‑1 (MQ=255)                                                                                  ggCGCAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCATCGAAAAACGTAAACCTAA‑TTTCGTGGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAAATCAGAGGAAAAGGATGACCGCAAATGAACCGGCGAAAATTATCCGGAAAAAAGACAGGGGGGCAttaaaa                                                                                                                                                           >  2:668168/1‑235 (MQ=255)                                                                                  ggCGAAGCACACC‑C‑CAAGAACTCCGAATAATTTGAACGTACTCGGGGAATGCC‑CCGCGGGGTGTATGACAGCGAAGATTCCAAGGAAGGGATGAACGCTTTCCTCGAAAACCGTAACCCTAA‑TTTCTTTGGTCATTAA‑CCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGGGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAg                                                                                                                                                            <  1:78005/238‑1 (MQ=255)                                                                                   gCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCg                                                                                                                                                          >  1:90052/1‑239 (MQ=255)                                                                                   gCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGCATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAACGGCAGAAATTATCCAGCATAACGACAtgg                                                                                                                                                                      >  1:262116/1‑227 (MQ=255)                                                                                       aGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGAAGTAAAAATGGAAACTCAGTGGCCAAGGAGGACCGCCAATGAAGCGGCAGAAATTATCCAGAATAACGAACAGGTGCAATTTAAGGGCtt                                                                                                                                                      >  2:302658/1‑239 (MQ=255)                                                                                            aca‑c‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCa                                                                                                                                                                                                                              >  2:53844/1‑162 (MQ=255)                                                                                            aca‑c‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCa                                                                                                                                                                                                                              <  1:53844/162‑1 (MQ=255)                                                                                                   aTGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCcggcg                                                                                                                                            >  1:588726/1‑239 (MQ=255)                                                                                                     gAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAAt                                                                                                                                                                                                        >  1:546866/1‑177 (MQ=255)                                                                                                     gAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCggg                                                                                                                                          >  1:386618/1‑239 (MQ=255)                                                                                                            gATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATaa                                                                                                                                                                              >  1:288593/1‑196 (MQ=255)                                                                                                            gATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCg                                                                                                                                   >  1:395447/1‑239 (MQ=255)                                                                                                            gATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCGTTACCCCGGCGGGTTCGCCg                                                                                                                                   >  1:713777/1‑239 (MQ=255)                                                                                                              tGAATTTAAACGTATCCGGGGGAGGCG‑CCGGGCGGTGTAGGACAGCGAAGATTACAAGGAAGGGATGAACGCTTTCCTGAAAAAACGAAAACCAAA‑TTTCGTTGGTAATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGGGGAAAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGa                                                                                                                                  <  1:544421/238‑1 (MQ=255)                                                                                                              gaattgtaaaCGTATGGGGGGGGGGCC‑CCGCGGGGTGTAGGACAGGAAAGATTCCCAGGAAGGGATGAACGTTTCCCAAGAAAAACGTAAACCTAA‑TTTCGTGGGTCATTAA‑CCCCGGAGAACGAAGGAGAAAAAATGGAAACTCGGGGGAAAGGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGa                                                                                                                                  <  2:90052/230‑1 (MQ=255)                                                                                                                     gAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGGGGCATTTAACGGCTTTACCCCGGCGGGTTCGCCGAAAGACCt                                                                                                                           >  1:845739/1‑238 (MQ=255)                                                                                                                          tATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAACTTCAGTGAACAAGGAGAACAGCCAATGAAGCGGCAGAAATAATCCAGAAGAACGACATGTTGGCCTTTAGAGGCGTTCCGCCGGCGGTTTGACCGAAAACCCTcccaa                                                                                                                      >  1:36466/1‑236 (MQ=255)                                                                                                                                    aTGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGGAAGCCCTACCCACCCCGATTGc                                                                                                            >  1:1068154/1‑238 (MQ=255)                                                                                                                                     tGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCCTTTAGCGGCTTTACCCCGGCGGGTTCGCCGGAAGCCCTCCCCACCCCGGTTGcc                                                                                                           >  1:208105/1‑238 (MQ=255)                                                                                                                                     tGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTTGCGCAAAGCCCTACCCCCCCCGATTGcc                                                                                                           >  1:443921/1‑238 (MQ=255)                                                                                                                                     tGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTTCCCACCGCGATTGcc                                                                                                           >  1:973751/1‑238 (MQ=255)                                                                                                                                     tGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGcc                                                                                                           >  1:61287/1‑238 (MQ=255)                                                                                                                                     tGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCCGGGGGGTCGCCGAAAGCCCCACCCCCCCGGATAGcc                                                                                                           >  2:55418/1‑238 (MQ=255)                                                                                                                                     tGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGGGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGCAAACCCTAACCACCGCGATTGcc                                                                                                           >  2:418530/1‑238 (MQ=255)                                                                                                                                      gcg‑cCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGCCATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCCCCCAAAACCCCACCCACCCCGATTcccc                                                                                                          >  2:1039408/1‑238 (MQ=255)                                                                                                                                      gcg‑cCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTACCGGCTTTCCCCCGCCGGGTTCGCCGAAACCCCCACCCACCGCGGTTGccc                                                                                                          >  2:1016044/1‑238 (MQ=255)                                                                                                                                             cgcgGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGTCATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCCCCGCGGTTGcccccccagc                                                                                                   >  1:11024/1‑232 (MQ=255)                                                                                                                                             cgcgGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCCCAGAGc                                                                                                   >  1:990039/1‑239 (MQ=255)                                                                                                                                                  tgtATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTAGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAACCCCTACCCACCCCGATTGCCCCTAGAGCTAACg                                                                                              >  1:650641/1‑239 (MQ=255)                                                                                                                                                     aacacaggaaaGTTTACCAGAAAGGGAAGAACGTTTTCCACAAAAACCGTAAACCTAA‑TTCCCTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGAAAAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAAc                                                                                           <  1:49831/230‑1 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGCAATTATCCAGCATAACGACATGGTGGCCTTTAGCGGCTTTACCCCGCCGGGTTCGCCGAAAGCCCTACCCACCCCGATTTCCCCCAAGGCTAACGaaaacaaaga                                                                                    >  2:318242/1‑231 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCTCCGAAAGCCCTACCCACCCCGATTGCCCCCCGAGCTAACGAACACCAAGa                                                                                    >  2:212990/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCCCCGCGATTGCCCGCAGCGCTAACGAACAGCATGa                                                                                    >  1:246560/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCCGGGCTAACGAACAGCATGa                                                                                    >  1:609643/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCCGAGCTAACGCACAGCATGa                                                                                    >  1:788398/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGa                                                                                    >  1:794872/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCAACCCCGATTGCCCGCCAGGCTAACGAACAGCAGGa                                                                                    >  1:116829/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAACCCTACCACCCCCGATTGCCCGCCGACCTAAAGAAAAGCAAGa                                                                                    >  2:393068/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCCAAAGCCCTAACCACCCCGAATGCCCCCAGGACAAAAGAAAAGCAAGa                                                                                    >  2:384678/1‑239 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGACGAAAGCCCTACCCACCGCGATTGCCCGCTAGGCCAACGAACACCATgg                                                                                    >  1:438259/1‑238 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCCCCGAAAGCCCCACCCACCGCGATTCCCCCCAAGGCAAACGaaaaaccaag                                                                                    >  2:868842/1‑231 (MQ=255)                                                                                                                                                            cGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATAGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGACAACGAACAGCCTGa                                                                                    >  1:696687/1‑239 (MQ=255)                                                                                                                                                              aaGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGCAGCGGCAGAAATCATCCAGCATAACGACATGGGGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAACATgcg                                                                                   >  1:513559/1‑236 (MQ=255)                                                                                                                                                              aaGATTACCAGGAAGGGATGAACGATTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGTTGGCATTTAGCGGCTTTACCCCGGCGGGATCGCCGAAAGCCCTACCCCCCGCGATTGCCCGCAGAGCTAACGACCAGCATGAg                                                                                   >  1:555027/1‑238 (MQ=255)                                                                                                                                                                   tACCAGGAGGGGA‑GAACGCCTTCCCTAAAAAAACTAAACCCAATTTTCGTGGGTCAGTAA‑TCACTGCTAACGAGGGAGAAAAAGTGAAAACTCAGGGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCaaa                                                                             <  1:378347/239‑1 (MQ=255)                                                                                                                                                                           aGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGGTCGCCCAAAGCCCTAACCACCGCCGATGCCCCCAGGGGCAACGGACACAATAAGGCaaaaaaccacaa                                                                     >  2:679189/1‑233 (MQ=255)                                                                                                                                                                           aGGGATAACCGCTTTCCGAGAAAAACGTAAACTTAA‑TTTCGTTGGTCATTAA‑CCCCTGCGAACGAAGGAGAAAAAATGAAAACTAAGGGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTa                                                                     <  1:382143/239‑1 (MQ=255)                                                                                                                                                                             ggATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAACTTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCCACGCAAGCCCTACCCCCCCCGATTGGCCGCCGACCTAACGACCACCCAGAGGCCAACAAcccacata                                                                   >  2:819556/1‑234 (MQ=255)                                                                                                                                                                               aTGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCaa                                                                 >  1:258470/1‑239 (MQ=255)                                                                                                                                                                               aTGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGACTTCATCGGCTTTACCCCGGCGGGTTCGCCGAAAACCCTACCCACCGACGTTGCCCCACGACCTAACGAACACCATACGGCCaaaaaacgggttcaa                                                                 >  1:1057158/1‑229 (MQ=255)                                                                                                                                                                                       tttCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCt                                                         >  1:1004215/1‑239 (MQ=255)                                                                                                                                                                                        ttcgtgaaaaacCGAAAACCAAG‑TTTC‑TTGGGCATTAACCCCCTGGAAACGAAGGAGTAAAAAGAGAAACTCAGTGGCCAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCtt                                                        <  1:1016044/232‑1 (MQ=255)                                                                                                                                                                                         tCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAACCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTTTCAAATTCGCCTTc                                                       >  1:309215/1‑239 (MQ=255)                                                                                                                                                                                               aaaaaataaaaCTTTA‑TTTCGTTGGCAATTAA‑CCCCGGCGAACAGAGGAAAAAAAAGGGAAACCCAGTGGAAAAGGAGGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACgg                                                 <  2:258470/231‑1 (MQ=255)                                                                                                                                                                                               aaaaaCTAAAACCTAA‑TTTCGTTGGTCATTAA‑CCCCTACAAACGAAGGAGAAAAAAGGGAACCTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACgg                                                 <  1:747892/239‑1 (MQ=255)                                                                                                                                                                                                     gTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCCCAGAGCTAACGAACAGCCTGAGGCCAAAAAGCCGGATCAAATTCGCCCTCTGACagggcggt                                           >  2:649457/1‑234 (MQ=255)                                                                                                                                                                                                              a‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCCACGAAAGCCCTACCCCCCGCCATTGCCCGCCGCGCTAACCAACAGCATGAGGCCAAAAAGCCGCATCAAATTCCCCTTCTGCAGGGGGCGCCAACCAgc                                   >  2:598706/1‑238 (MQ=255)                                                                                                                                                                                                               gttttgggggTAATAAA‑CCCCTGCGAACAAAGGAAAAAAAAGGAAAACCAAGGAGAAGGGCAGGCCCGCCAATGAAGCGGCAGAAATTCTCCAGCATAACGACATGGTGGCATTTAGGGGCGTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCCCCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAgcg                                  <  2:619372/231‑1 (MQ=255)                                                                                                                                                                                                                     ttGGTCATTAA‑TCCCTGCGAACGAAGGAGTGAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGcgccgcc                             >  1:622305/1‑238 (MQ=255)                                                                                                                                                                                                                     caagTCATTAC‑GCCCGGAAAAAGAAGGAAAAAAAAGAGAATCACAGGGGAAAGGGATGCCCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGcgccgccg                            <  1:1034545/236‑1 (MQ=255)                                                                                                                                                                                                                       ggTAATTAA‑CCCCGGAGAACAAAGGAAAAAAAAGGAAAACACGGGGGAAAGTGGTGTCCGGCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCCCCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGcgccgccgc                           <  1:629744/238‑1 (MQ=255)                                                                                                                                                                                                                                   ccTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGc                                                           >  1:349029/1‑195 (MQ=255)                                                                                                                                                                                                                                     tGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCCCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCCGATCAAATTTGCCCTCCGACGGGGGGGGCAAACAGCGCCCCCCCcgacgaaat                  >  2:1034545/1‑231 (MQ=255)                                                                                                                                                                                                                                     tGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAACCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAACCGTATCAAATTCGCCTTCTGCCGGGGGCGTCAATCAGCCCCGCCCCCGCCAAGGTACttt             >  2:306124/1‑239 (MQ=255)                                                                                                                                                                                                                                      gCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAACCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCATCTCCGCCGCGACCGATGTACtt              >  1:599424/1‑237 (MQ=255)                                                                                                                                                                                                                                                 aGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCCGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCCCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGCGGAACTTTCTGACCCCGAt  >  1:453640/1‑238 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            TTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACA‑C‑CATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCG‑CCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAA‑TTTCGTTGGTCATTAA‑TCCCTGCGAACGAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGCCGAT  >  NC_000913/3064560‑3065032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑