Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,020,961 A→G intergenic (‑42/‑177) sulA ← / → sxy SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,020,9610AG98.8% 267.0 / NA 84intergenic (‑42/‑177)sulA/sxySOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (44/38);  total (46/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTAAT  >  NC_000913/1020822‑1021097
                                                                                                                                           |                                                                                                                                        
aTAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCAt                                                                                                                                       >  1:254732/1‑143 (MQ=255)
     cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGt                                                                                                                                  >  1:195334/1‑143 (MQ=255)
       cGGCTGTAGTGTTTTCCGGAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGACGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGa                                                                                                                                <  2:3169/143‑1 (MQ=255)
          ccgTAGTGTTTGCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGacaa                                                                                                                             <  1:761242/141‑1 (MQ=255)
               ttgtgTTCCGTAGGGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAg                                                                                                                        <  2:386250/142‑1 (MQ=255)
               gtgtTTTCCGTAGAGACACGCGAAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAg                                                                                                                        <  2:1007859/143‑1 (MQ=255)
                 gtTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGAt                                                                                                                      >  1:1122644/1‑143 (MQ=255)
                    ttCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGCATGTACAGTGCATCCAGTGACAACACAGACCaa                                                                                                                   >  2:309763/1‑143 (MQ=255)
                     tCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAAc                                                                                                                  <  2:1191119/143‑1 (MQ=255)
                     tCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAAc                                                                                                                  <  1:690933/143‑1 (MQ=255)
                     tCCGGAGAGACACGCGCAATTTCACTGGCTGCGGATGAGAACGACGAAGAACGATTTGCATAGCCTGAAGTGTACATAATCCATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCTAGTGACAACAAAGATCAAc                                                                                                                  <  2:1102999/143‑1 (MQ=255)
                      ccGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAAcc                                                                                                                 >  1:1041288/1‑143 (MQ=255)
                        gTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTCCAGTACACCAAAACACAACAAAGATAAACCCt                                                                                                               >  2:289174/1‑143 (MQ=255)
                             gaCACGCGCAATTGTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTAtttt                                                                                                          >  1:882179/1‑143 (MQ=255)
                              acacGCGGAATTTTACTTGCTGCGGATGAGAACGACGAAGAGCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTc                                                                                                         <  2:1153129/143‑1 (MQ=255)
                              acacGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTc                                                                                                         <  2:1137853/143‑1 (MQ=255)
                                  gcgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCGTGAAGTGTACATAATCAATCCAGCAAATCTGAGGTACTGTATCGAAGATGTTTTCATCCAGTGACAACAAAGATCAACCCTATTTTCGGaa                                                                                                     >  1:163353/1‑143 (MQ=255)
                                  gcgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCggca                                                                                                     >  1:398062/1‑141 (MQ=255)
                                   cgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGaaa                                                                                                    <  1:369234/143‑1 (MQ=255)
                                    gcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTCCGGAAag                                                                                                   >  2:1113439/1‑143 (MQ=255)
                                        ttttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGcc                                                                                               >  2:272037/1‑143 (MQ=255)
                                          ttACTTGCAGCGGATGACAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTc                                                                                             <  1:338656/143‑1 (MQ=255)
                                                gctgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCACAt                                                                                       >  1:304357/1‑143 (MQ=255)
                                                gctgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAt                                                                                       <  1:588141/143‑1 (MQ=255)
                                                 ctgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAtt                                                                                      <  2:1041288/143‑1 (MQ=255)
                                                    cGGATGAGAACGACGAAGAACTATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAGTTTTg                                                                                   >  1:799979/1‑143 (MQ=255)
                                                      gATGAGTACGATGATGAACGATGTGCATAGCCTGAAGTGTACATTATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTc                                                                                 <  2:928744/143‑1 (MQ=255)
                                                       aTGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCg                                                                                >  1:720291/1‑143 (MQ=255)
                                                        tGATAACGGTGAAGAACGATGTGCTTAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTGACTGTATGGATGTACAGTGCAGCCAGTGACAACAAAGATCCACCCAATTTCCGGAAAGAGCCTCGCTAATTTTGTCGt                                                                               <  2:1125181/143‑1 (MQ=255)
                                                         gggaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACCACAAAGATCAACCCTATTTTCGGAATGAGCCTCGCAAATTTTGTCGtt                                                                              <  2:357352/141‑1 (MQ=255)
                                                           gaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACTATATTTGTGGAAAGAGCCTCGCAAATTTTGTCGTTgg                                                                            >  1:218858/1‑143 (MQ=255)
                                                           gaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTgg                                                                            >  1:706926/1‑143 (MQ=255)
                                                             acgacgAAGAACGATGTGCATAGCCTGAAGTGTACATAATAAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGta                                                                          >  2:1163615/1‑142 (MQ=255)
                                                                  gaagaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTCTGGATATACAGAGCATCCAGTGATAACAAAGATCAACCCTATTTTCAGAAAGACCCTCGAAAAATTTGTCGTGGGAGACggg                                                                     >  2:787060/1‑143 (MQ=255)
                                                                  gaagaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTCGTCGTTCGTCACgtg                                                                     >  2:1066467/1‑141 (MQ=255)
                                                                  gaagaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATCTTCGGAAAGAGCCTCGAAAATTTTGTCGTTGGTGCCggg                                                                     >  2:235890/1‑143 (MQ=255)
                                                                       aCGATGTGCATAGCCTGAAGTGTACATAATCAGTCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAAc                                                                <  1:321546/143‑1 (MQ=255)
                                                                         gATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACAt                                                              >  1:814547/1‑143 (MQ=255)
                                                                           tgtgCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACGGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATaa                                                            <  2:845182/143‑1 (MQ=255)
                                                                              gCATAGCCTGAAGTGGACATAAGCCACCCAGCCCCGGTGAGTTACGGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAAtt                                                         <  1:813243/143‑1 (MQ=255)
                                                                                    ccgtcaGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCtt                                                   <  1:113998/138‑1 (MQ=255)
                                                                                    ccTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAACGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAACTAATCtt                                                   >  2:98582/1‑143 (MQ=255)
                                                                                     cttaaGTGTACACACTCATTCCACCCCCTGCGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACTAAGATCAACCCTATTTTCGGAAAGCGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTg                                                  <  2:975718/140‑1 (MQ=255)
                                                                                     cTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGACAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTg                                                  >  2:355989/1‑143 (MQ=255)
                                                                                      tGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGc                                                 >  1:461351/1‑143 (MQ=255)
                                                                                       gAAGTGTACATAATCACTCCAGCGCCTGGGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGcc                                                <  2:491986/143‑1 (MQ=255)
                                                                                        aaGTGTACATAATCAATCCAGCCTCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGccc                                               <  1:153716/143‑1 (MQ=255)
                                                                                        aaGTGTACACAAGCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAGGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGccc                                               <  1:445524/143‑1 (MQ=255)
                                                                                           tgtACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTGCCAAATTTCGCCGCTGATGACGGGAAGACGCAAAGTACTCCTACCCCta                                            >  2:969670/1‑142 (MQ=255)
                                                                                            gcaCATAATCACTCCAGCCCCTGTGGGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTa                                           <  1:636725/141‑1 (MQ=255)
                                                                                             tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTaa                                          <  1:207465/143‑1 (MQ=255)
                                                                                             tACATAAGCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTaa                                          <  2:449958/143‑1 (MQ=255)
                                                                                              aCATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAg                                         <  2:706926/143‑1 (MQ=255)
                                                                                               cATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAGAGCATAAATTAATCTTGACACTTAAGa                                        >  1:1122459/1‑143 (MQ=255)
                                                                                                aTAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGac                                       >  1:1101295/1‑142 (MQ=255)
                                                                                                  aatcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTATCGAAAAGAGCCTCGCATGTGGAGACGGAGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATa                                     >  1:503497/1‑143 (MQ=255)
                                                                                                   atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATaa                                    <  1:633641/143‑1 (MQ=255)
                                                                                                   atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATATTCGGAAAGAGCCTCGCAACTTTTGTCGTTGGTGACGGGAAAACATAGATTAATCATGCCCGTTGAgaagaa                                    >  1:1034933/1‑143 (MQ=255)
                                                                                                   atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCGATTTTCGGAAAGAGCCTCGCAGAAACTAAAGCTGGTGACGGGGCGACAGACAATAATCTTGCCCCTTCAGAATaa                                    >  1:1066699/1‑143 (MQ=255)
                                                                                                   atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGAGCAACCCTATTTTCGGAAAGAGCATCACAAATACGGTCGTTGTAGACGGGAAAGCCTAAATTAATCTTGCCCCCTAAGagtca                                    >  2:530393/1‑139 (MQ=255)
                                                                                                   accactcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATaa                                    <  1:53906/138‑1 (MQ=255)
                                                                                                    tcaatcCAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAg                                   >  1:661021/1‑143 (MQ=255)
                                                                                                        tccttccccTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACACAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGc                               <  2:1026022/138‑1 (MQ=255)
                                                                                                          cAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCt                             >  1:629791/1‑143 (MQ=255)
                                                                                                          cAGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCt                             >  1:735465/1‑143 (MQ=255)
                                                                                                           aGCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCATTGGTGACGGGAAAACATAAATTAATCTTGCACCTTAAGAATAAGTTGCCTa                            >  2:210187/1‑143 (MQ=255)
                                                                                                            gCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTAt                           >  2:495206/1‑143 (MQ=255)
                                                                                                            gCCCCTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTAt                           >  1:94227/1‑143 (MQ=255)
                                                                                                             ccccTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAACTTTTGTCGTTGGTGACGGGAAAACATAAATTACTCTTTCCCCTTAAGAATAAGTGGCCTaat                          >  2:434040/1‑141 (MQ=255)
                                                                                                              cccTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTAttt                         >  1:513837/1‑143 (MQ=255)
                                                                                                               ccTGTGAGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTAtttt                        >  2:870529/1‑143 (MQ=255)
                                                                                                                cTGGGAGGTACTGTAGGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAGTTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTc                       <  1:270179/143‑1 (MQ=255)
                                                                                                                   tgAGTTACTGTGCGGATCTACAGTGCATCCAATTACAACAAAGATCAACCCTATTTTCGCAAAGAGCCTCGCAAATTTTGTCGTTGGTGCCGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa                    <  2:895496/143‑1 (MQ=255)
                                                                                                                   tgAGTTACTGTATGGATGTCCAGTGCTTCCAGTTGCAACAAAGATTAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCATATTTTCGTa                    <  2:895488/143‑1 (MQ=255)
                                                                                                                   tgAGGTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa                    <  1:589005/143‑1 (MQ=255)
                                                                                                                     aGTTACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATACGTTGCCTATTTTGGTAGt                  >  2:234276/1‑143 (MQ=255)
                                                                                                                       ttACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATCAGTTCCCTATTTTCTTGGTTa                >  2:545048/1‑143 (MQ=255)
                                                                                                                       ttACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa                >  2:919575/1‑143 (MQ=255)
                                                                                                                       ttACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCACCTTAAGAATAAGTCGCCTATTTTCGTAGtaa                >  2:7606/1‑141 (MQ=255)
                                                                                                                        tACTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTaa               >  1:1180509/1‑143 (MQ=255)
                                                                                                                         accgTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAAc              <  1:291026/140‑1 (MQ=255)
                                                                                                                         aCTGTATGGATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAAc              <  1:77632/143‑1 (MQ=255)
                                                                                                                           tGTATGGATGTACAGTGCATACAGTGACAACAAAGATCAACTCTATCCACGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGGAAACATAAATTAATCTGGCCCCTTAAAAATAAGTTGCCTACTTTCGTAGTTGACgg            >  1:757677/1‑143 (MQ=255)
                                                                                                                                 gATGTACAGTGCATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGt      <  2:799979/143‑1 (MQ=255)
                                                                                                                                     ttcagtgcaTTCAGTGTCATTAAAGTTCATCCTTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGTCGGGAAAACATATATTAATCTTGCCCCTTAAGAATAATTTGCCTATTTTCGTAGTTAACGGATCCGTTAAt  <  2:1087300/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
ATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGTTAAT  >  NC_000913/1020822‑1021097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: