Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	98.1%	161.5 / NA	53	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (23/29);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132652‑2132926                                                                                                                                        |                                                                                                                                            tGGTAGAGGGGAAACCGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGt                                                                                                                                      <  1:762781/143‑1 (MQ=255)   gtagccggTAAACAGCTGATATCTTTGGCTGGCGCGGGCGAATTTGCCTTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                    <  1:819720/137‑1 (MQ=255)       agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGCTCTGCATTTCACCGTGTTTGATATGCAGGCTCTGCGTCGCctc                                                                                                                                >  1:449725/1‑143 (MQ=255)       agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc                                                                                                                                <  1:1080677/143‑1 (MQ=255)       agGGTAAACAGCGGAGATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTGCGTCGCctc                                                                                                                                <  1:972510/143‑1 (MQ=255)            taaCCGCTGGTATTTGTTGCTGGCGCGGTCGAGTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                           <  2:1124471/142‑1 (MQ=255)            aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                           <  1:204455/143‑1 (MQ=255)            aaaCAGCTGATATTTTCTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                           <  1:137990/143‑1 (MQ=255)                 gCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGTAGAATTTTTTCGGTGACGAAGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACttgc                                                                                                                      >  2:401977/1‑141 (MQ=255)                 gCTGATATTTTTTGCTGGCGCGGTAGAATTAGTCTTACTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATGAGCAGGATCTGCGGCGCCTCTTCTAcgtac                                                                                                                      >  2:401670/1‑139 (MQ=255)                   tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    >  1:774103/1‑143 (MQ=255)                            ttGCTGGCGCGGTCGAATTTGGCGTTGTGCGTGCGGCAAAAGTGGAAGTACCCGGAGAATTTTTTCTGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                           <  1:492934/143‑1 (MQ=255)                               cTGGCGCGGTCGAATGTGTCGGTGTGCTTGCGGGAAAAGTGGAAGTGCCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACGTTCCACGGTTCGCCGTa                                                                                                        <  1:376512/143‑1 (MQ=255)                               cTGGCGCGCTCGAATTTGTCTTTGTGCTTGCGGTCAACGTGCAAGTACCCGTATAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                        <  1:100066/143‑1 (MQ=255)                                tGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAGCTAGAAATATTCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                       >  1:220697/1‑143 (MQ=255)                                tGGCGCGGTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATCTGCAGGCTCTGCGCCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                       <  1:855320/143‑1 (MQ=255)                                  gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTc                                                                                                     >  2:1035042/1‑143 (MQ=255)                                                   tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCGACCACCAGCCGCCGGa                                                                                    >  2:818640/1‑143 (MQ=255)                                                    ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                   <  1:812313/143‑1 (MQ=255)                                                    tggtgCTTACTGTGAAGGTCGAAGTACCCTGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCTCCGGGATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                   <  2:587597/141‑1 (MQ=255)                                                     tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGTTCGCCATACTTCCTCACCACCAGCAGGaac                                                                                  >  2:687183/1‑142 (MQ=255)                                                     tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCGTCCGCAggccg                                                                                  >  1:197304/1‑140 (MQ=255)                                                     tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTCCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                                  <  1:249764/143‑1 (MQ=255)                                                               gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAt                                                                        >  1:609472/1‑143 (MQ=255)                                                                  caaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt                                                                     <  1:770954/142‑1 (MQ=255)                                                                     gTGGAAGTACCCGGAGAATTTTTTCGGTGACGGGGTGATCTGCATTTCACCGTGATTGATATGTAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCCTACTCCCCCCCAATCCGCACGACGAGGTCGTAATCCtgtg                                                                  >  2:862012/1‑143 (MQ=255)                                                                          aggtcctggAGATTTTTTTAGGTGACGAGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt                                                             <  2:994786/136‑1 (MQ=255)                                                                            tggccGGAGAATGTGTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                           <  1:388864/140‑1 (MQ=255)                                                                                 ggAGAATTTTTTCGGTGACGACGTCATCTGCATTTCACCGTGATTGATATGCAAACTCTTCGTCGCCTCTTCTACTTTCCACGCTTCGCCGTAGCTCAACAACATCCGCAGAAAGCTCTGCTAATTCTGTGCCGCtctaccgc                                                      >  2:529200/1‑136 (MQ=255)                                                                                     aaTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt                                                  >  2:639945/1‑143 (MQ=255)                                                                                       ttttttCGGTGGCGAGCGGATCGGCCTTTCTCGGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                <  2:904110/143‑1 (MQ=255)                                                                                        tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAAGGTTCGCCGTACTCCACCACCATCCGTCGTAAGATGTCGTAATCCTGTCCCGCTTGCAGTGCGGTAta                                               >  2:808651/1‑142 (MQ=255)                                                                                           ttCGGTGACGAGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGAAATCGTGTGCCGCTTTCAGTTCGGTATCGaa                                            <  2:413489/143‑1 (MQ=255)                                                                                               gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTGTCGaacaaa                                        >  1:582672/1‑143 (MQ=255)                                                                                                  aCGAGGTGATCTGCGTTTCACCGTGATTGATATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac                                     <  2:315835/143‑1 (MQ=255)                                                                                                    gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTGCCGTTCGGTACCGaacatacaacc                                   >  1:391785/1‑140 (MQ=255)                                                                                                    gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                                   >  1:126654/1‑143 (MQ=255)                                                                                                     aGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGTCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct                                  <  1:608920/143‑1 (MQ=255)                                                                                                             tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGGATCGAACAAACACTCTTTGAAACg                          >  1:582600/1‑143 (MQ=255)                                                                                                             tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCACAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg                          >  1:775012/1‑143 (MQ=255)                                                                                                                     aCCGTGATTGATATGCAGGCGCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                  <  1:1122013/143‑1 (MQ=255)                                                                                                                      ccGTGATTGATGTGCAGGCTCTGCATCGCCTCCTCCACTTTCCACGGTTCTCCGTACTCCACCTCCATCTGCAGGAAGATCGAGCTATCGTGCCTTACCATCACCGCCGCATCGAACAAACACTCTTTGAAACTCCATGCCCa                 <  2:518244/143‑1 (MQ=255)                                                                                                                      ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCACAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcccg                 >  1:388799/1‑142 (MQ=255)                                                                                                                      ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCCCCATCCGCAGGAAGATGTCGTACTCTTGTGCCGCTTTCAGTACGTTATAGAACAAAAACTATATAAAACGACATGccaa                 >  1:135809/1‑141 (MQ=255)                                                                                                                      ccGTGATTGATATGCAGGCCCTGCCTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa                 <  1:1016133/143‑1 (MQ=255)                                                                                                                          gattgatGTGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGTTTGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTa             <  2:86823/143‑1 (MQ=255)                                                                                                                           attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCACCATCCGCAGGAAGATGTCGTAATCCTCTGCCGCTTTCAGTTCGGTATCGCACAACCACTCTTTGCAACGCCATCTCCAGGtca            >  1:680113/1‑141 (MQ=255)                                                                                                                            ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAGTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa           <  1:976620/143‑1 (MQ=255)                                                                                                                              ggtatGCAGGCTCTGCGTCGCCTCTGCTACTTTCCGCGGTTCGCCGTCCGCCACCACCATCCGCAGGAAGGTGTCGTAATCCTGTGCCGCTTTCAGGTCGGGATCGAACAAACACTCCTCGAGATGCCATGCCCAGGTAAAGa         <  2:1014834/141‑1 (MQ=255)                                                                                                                              cgtctGCAGGCTCCGCGTCGCCTCCTCTACTTTCCACCGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAACCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACGCTTTGAAACGCCATGCCCAGGTAAAGa         <  1:1050037/139‑1 (MQ=255)                                                                                                                                 atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt      >  1:553034/1‑143 (MQ=255)                                                                                                                                 atGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt      <  1:712619/143‑1 (MQ=255)                                                                                                                                     aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGAAAAGACCTGGtt  >  2:1013128/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                            TGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132652‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑