Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	711,536	C→T	intergenic (‑71/+69)	fldA ← / ← ybfE	flavodoxin 1/LexA‑regulated protein, CopB family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	711,536	0	C	T	100.0%	160.9 / NA	56	intergenic (‑71/+69)	fldA/ybfE	flavodoxin 1/LexA‑regulated protein, CopB family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/29);  total (27/29)

GAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCT  >  NC_000913/711399‑711670                                                                                                                                          |                                                                                                                                       gAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGTATAAAGGCAATCATAGTAGAAc                                                                                                                                   >  2:900584/1‑143 (MQ=255)    tgtgttttGCGATATTCTCGTCATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGTGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAgg                                                                                                                                <  2:288373/139‑1 (MQ=255)    tCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGCTAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAgg                                                                                                                                <  2:592614/143‑1 (MQ=255)    tCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAgg                                                                                                                                <  2:589835/143‑1 (MQ=255)      aTTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATCGTAGAACAGGcg                                                                                                                              >  2:1002550/1‑142 (MQ=255)      aTTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATCGTAGAACAGGCa                                                                                                                              >  1:876666/1‑143 (MQ=255)      aTTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCa                                                                                                                              <  2:73650/143‑1 (MQ=255)       tttttGCGATATTTTCGGTATTACCGGTGTCGCGGCCGAGAAAAATGCCATTGATAGCCATGAGTGAAATCACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAg                                                                                                                             <  2:399131/143‑1 (MQ=255)             cGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGGGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGc                                                                                                                       <  1:393474/143‑1 (MQ=255)              gATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGCAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCg                                                                                                                      <  1:962526/143‑1 (MQ=255)              gATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCg                                                                                                                      >  1:612912/1‑143 (MQ=255)               atatTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTCAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCGTAGTAGAACAGGCAGTCTTGCgg                                                                                                                     >  1:585090/1‑143 (MQ=255)                  ttttCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTAGAAACTTATTGAAATGAGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAAt                                                                                                                  >  2:23505/1‑143 (MQ=255)                   tttCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGACATGACTGAAATAACATCTTTAAACTTATCGAAAAGGAGGTGGAAAATTGCCCACGGCCAACGGCAAGCCGAGTAGAACAGCCAGACTTGCGGAATc                                                                                                                 >  2:17281/1‑143 (MQ=255)                     tCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAg                                                                                                               <  1:226794/143‑1 (MQ=255)                         tATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCaaa                                                                                                           >  1:909812/1‑143 (MQ=255)                           ttACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGGCAAGCGCCCACAGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACg                                                                                                         >  1:533995/1‑143 (MQ=255)                            tACCGGTGTCGCTGCCGAAAAGGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGa                                                                                                        <  2:381425/143‑1 (MQ=255)                              ccGGGGTCGCGGCCGAGAAAGATGCCAGTGATAGCGATGCGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAAGTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGc                                                                                                      <  2:365199/143‑1 (MQ=255)                                   gtCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGAc                                                                                                 >  1:865027/1‑143 (MQ=255)                                      gCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGc                                                                                              >  2:362510/1‑143 (MQ=255)                                                   aTGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCAAAGGGTAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCCAACGAGCAGGACTGCCCACTGTGATACa                                                                                 >  1:593342/1‑143 (MQ=255)                                                    cgCCAGTGTCAGCCATGAGTGCAATAACCTCTTGAATCTCATTGAAATGGCGGTGGAAAACTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCATGACTGCACACTGTGCTACAt                                                                                <  2:665492/142‑1 (MQ=255)                                                     gCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATg                                                                               >  1:338779/1‑143 (MQ=255)                                                      ccAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGACATGGGGGTGGAAAATTGCCCACGGATCAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGCATCAGCAGACGAGCAGGACGGCACACTGTGCTACATGa                                                                              >  1:241024/1‑143 (MQ=255)                                                      ccAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGa                                                                              >  2:891902/1‑143 (MQ=255)                                                            agggCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTgg                                                                        <  1:111451/140‑1 (MQ=255)                                                               gCCATGAGTGCACTAACCTCTTGAGACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAAGCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGaaa                                                                     <  1:466832/143‑1 (MQ=255)                                                                ccATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCACTCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACCAGCAGGACTCCACACCGCCCCACCTGAAAGTGGaaac                                                                    >  2:283618/1‑142 (MQ=255)                                                                 cATGAGTGAAATAACCTCTTGAAACTTATTTGTATGGGGGGAGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGGGGaaagt                                                                   >  2:1012494/1‑141 (MQ=255)                                                                 cATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAAtt                                                                   >  1:735066/1‑143 (MQ=255)                                                                 cATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAAtt                                                                   >  1:676499/1‑143 (MQ=255)                                                                   tgtgTGAAATAACCTCTTGAAACGTGTTGAAATGCGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTa                                                                 <  2:1081563/140‑1 (MQ=255)                                                                    gAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTaa                                                                <  1:253460/143‑1 (MQ=255)                                                                     gggtaaaGAACCTCTTGAAACTTCTTGAAATGGGGGTGGAACATTGCCCTCGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTaaa                                                               <  2:485695/139‑1 (MQ=255)                                                                     aGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTTGAAATTTaaa                                                               <  1:633110/143‑1 (MQ=255)                                                                     aGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTaaa                                                               <  1:633351/143‑1 (MQ=255)                                                                     aGTGAAATAACCTCTTGAAACTTATTGAAACCGGTGTGTAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTaaa                                                               <  1:782843/143‑1 (MQ=255)                                                                     aGTGAAATAACCTCTGGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTaaa                                                               <  2:1020086/143‑1 (MQ=255)                                                                         aaaTAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCCGCAACCGAGCAGGACTCCACACTGTGCTACATGAAAGAGGAAAGTTAACCGAt                                                           >  2:914160/1‑143 (MQ=255)                                                                           aTAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGc                                                         >  1:755652/1‑143 (MQ=255)                                                                                ctctTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACCGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGa                                                    >  1:203983/1‑143 (MQ=255)                                                                                   ttGAAGCTTATGGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTa                                                 <  2:978559/143‑1 (MQ=255)                                                                                   ctGAAGCTTATGGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTa                                                 <  2:134398/142‑1 (MQ=255)                                                                                     gacaCTTATTGACATGGGGGTGGAAACTCGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACg                                               <  1:168654/140‑1 (MQ=255)                                                                                      aaaCTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAAAAGGCAGACTTGCCGACTCAGCAAACGAGCCGGACTGCACACAGTGCTACATGAAAGTGGAAATTTAAACGATGCCCGGACTACGc                                              >  2:761877/1‑143 (MQ=255)                                                                                       aaCTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAGCGATGCCCTGACTACGCa                                             >  1:274753/1‑143 (MQ=255)                                                                                                         tGGAAAATTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCa                           >  2:310824/1‑143 (MQ=255)                                                                                                             aaaTTGCCCACGGATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTaa                       <  1:874201/143‑1 (MQ=255)                                                                                                                 tgtccACGGGTAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACAtc                   <  2:1046332/140‑1 (MQ=255)                                                                                                                      aCGGATAAAGGCGATCATAGTAGAACAGGCAGTCTTGCGGAATCAGTAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTc              <  1:516501/143‑1 (MQ=255)                                                                                                                         gATAAAGGCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGAt           <  1:227979/143‑1 (MQ=255)                                                                                                                               ggCAGTCATAGTAGAACAGGCAGTCTTGCGGAATGAGCAGACGAGCAGGGCTGCACACTGTGCTACGTGTGGGTGGGAATTTAAACGATGCCCTGACTACGCAGCGCCGGCAGTTGCTGCATTGACATGTCTTCGATCGGtgc     >  2:612430/1‑141 (MQ=255)                                                                                                                               ggCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTc     >  1:331929/1‑143 (MQ=255)                                                                                                                                gCAATCATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCg    <  2:788635/143‑1 (MQ=255)                                                                                                                                  aagcATAGTAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTCAGCGGTTCCCTTACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCt  <  1:216790/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GAATCATTTTTGCGATATTTTCGGTATTACCGGTGTCGCTGCCGAAAAAGATGCCAGTGATAGCCATGAGTGAAATAACCTCTTGAAACTTATTGAAATGGGGGTGGAAAATTGCCCACGGATAAAGGCAATCATAGCAGAACAGGCAGTCTTGCGGAATCAGCAAACGAGCAGGACTGCACACTGTGCTACATGAAAGTGGAAATTTAAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCT  >  NC_000913/711399‑711670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑