BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,209,827	T→G	13.3%	noncoding (54/76 nt)	gltV →	tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,209,827	0	T	G	13.3%	198.2 / 2.4	107	noncoding (54/76 nt)	gltV	tRNA‑Glu
Reads supporting (aligned to +/- strand): ref base T (30/55); new base G (16/2); total (50/57)
Fisher's exact test for biased strand distribution p-value = 3.63e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGTTAAGCGACTAAGCGTACAC  >  NC_000913/4209589‑4210064

cTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGt                                                                                                                                                                                                                                                 >  1:866060/1‑239 (MQ=31)

cTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGt                                                                                                                                                                                                                                                 >  1:286742/1‑239 (MQ=31)

    ctcTTCCGGAGGGCGTTTACCACTTTGTGATTCATGACTGGTGTGAAGGCGTAACAAGGTGACCTTAGGTGAACCTGCTGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑Ga                                                                                                                                                                                                                                            <  2:1064739/237‑1 (MQ=11)

     ccTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑Gaa                                                                                                                                                                                                                                           <  1:1031658/239‑1 (MQ=31)

     ccTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑Gaa                                                                                                                                                                                                                                           <  1:626494/239‑1 (MQ=31)

     ccTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑Gaa                                                                                                                                                                                                                                           <  2:411462/239‑1 (MQ=31)

         cGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GGAtac                                                                                                                                                                                                                                        >  1:646557/1‑236 (MQ=14)

         cGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATcc                                                                                                                                                                                                                                        >  1:1009851/1‑238 (MQ=31)

         cGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATcc                                                                                                                                                                                                                                        >  1:459891/1‑238 (MQ=31)

               ggCGCTTACCACTTTTGGATTCATGACTGGGGTGAAGTCGTACCAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGCGCTCACACAGATTGTCTGATAGAAAGTGAAATGCAAGGCGTCTTGCGCAGCAGACTGCTACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGTG‑ACAAC‑GGGTTC‑TGATCCCCTCggg                                                                                                                                                                                                                                 >  2:694246/1‑239 (MQ=11)

                    ttACCACGTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACg                                                                                                                                                                                                                             <  2:1107314/238‑1 (MQ=14)

                     tACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGcc                                                                                                                                                                                                                           <  1:885973/239‑1 (MQ=31)

                           tttGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACtt                                                                                                                                                                                                                       >  1:32697/1‑237 (MQ=31)

                             tgtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGGAGCCACttgttg                                                                                                                                                                                                                   >  1:1079337/1‑239 (MQ=14)

                                    cATGCCTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTCTGATACCCCAGGGGGCCCCCACTTCCGGGTTtgt                                                                                                                                                                                                             >  2:137882/1‑239 (MQ=11)

                                    cATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCCCGGGGGAGCCACTTGCTGGTTtgtg                                                                                                                                                                                                            >  2:344730/1‑239 (MQ=9)

                                            gggTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGTCACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GGATCCCCTCGGGGACCCCCATTGCTGGTTtgttgaggtaag                                                                                                                                                                                                    >  1:839114/1‑230 (MQ=11)

                                            gggTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACCGCGGGTCC‑GAAT‑CCCTACGGGGCGCCCCTTCCTGGGTTGGGAGTGaaac                                                                                                                                                                                                    >  2:253186/1‑238 (MQ=11)

                                             ggTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAg                                                                                                                                                                                                    >  1:1108481/1‑238 (MQ=31)

                                              gTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGt                                                                                                                                                                                                   <  1:979714/238‑1 (MQ=31)

                                               tGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGG‑AACAG‑GGGCTC‑GCATCCCCTCAGGGGCCCCACTTCGTAGGTTgtgaggggaaatc                                                                                                                                                                                                  >  2:209285/1‑230 (MQ=11)

                                                gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc                                                                                                                                                                                                <  1:201225/239‑1 (MQ=31)

                                                gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc                                                                                                                                                                                                <  2:236257/239‑1 (MQ=31)

                                                gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc                                                                                                                                                                                                <  1:1083635/239‑1 (MQ=31)

                                                gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc                                                                                                                                                                                                <  1:427766/239‑1 (MQ=31)

                                                gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCGTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGTGGG‑AACAG‑GGGTTC‑GAATCGCTTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGGCAc                                                                                                                                                                                                <  2:688173/239‑1 (MQ=9)

                                                  aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAcc                                                                                                                                                                                               <  1:165295/238‑1 (MQ=31)

                                                      gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATcccc                                                                                                                                                                                                                                      >  1:980266/1‑195 (MQ=31)

                                                      gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCAc                                                                                                                                                                                                <  2:576462/233‑1 (MQ=31)

                                                      gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGC‑AAAAG‑GGGTTC‑GAATCCCCCTGGGGGAGCCCCTTTCTGGTTTGTGAGTGGAAGTTACCTGc                                                                                                                                                                                            >  1:1054486/1‑237 (MQ=11)

                                                       tAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAAGGGACGCCACTTGCTGGTTTGTGAGTGGAAGACACCTGCCtt                                                                                                                                                                                         >  2:954896/1‑239 (MQ=14)

                                                              gTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACCG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTTATAtc                                                                                                                                                                                   >  1:596896/1‑238 (MQ=14)

                                                               tAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATAtct                                                                                                                                                                                  <  2:459891/238‑1 (MQ=31)

                                                                 aCCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCaa                                                                                                                                                                               <  1:1154619/239‑1 (MQ=31)

                                                                    ggatgtgaagttGCGGTTGGATCACCTCCTTGCCTTAAAGACGTGTTCTTGGCAGTGCTCACGCAGATTGACTGATAGAAAGTGAAGAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTACGTCTATAGGCCCAGGACACCGCCCTTTCCCGGCGGT‑ATCAG‑GGATTC‑GAATCCCCTAGGGGACGCCACTTGCTTGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAAc                                                                                                                                                                            <  2:1066031/228‑1 (MQ=11)

                                                                    gTAGGGTAACGTGCGGTTGGATCACCTCCTTACGTTAAAGCAGCGTTCTGTGCAGTGCGCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCGTGCGAAGCAGACTGATAGGTCCCCTTCGTCTAGAGGCCCGGGACACCGCCCGTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATACCTCAAAAc                                                                                                                                                                            <  2:637734/239‑1 (MQ=11)

                                                                       ggggAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGC‑AACCG‑GGGGTC‑GAATCCCCCAGGGGACGCCACCTGCTGGTTTGTGAGGGAAAGTCCCACGCCTTAATATCCTaaaaat                                                                                                                                                                           >  2:770175/1‑235 (MQ=11)

                                                                             ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GCATCCCCCAGGGGAAGGCCCCTGCTGGTTTGGTGGGGAAAGTCACCCTGCTTAAATTCTCAAAACTCCTCtttg                                                                                                                                                                   >  2:911313/1‑237 (MQ=11)

                                                                             ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAAACAG‑GGG‑TC‑GAATCCCCTAGGGGACGCCACCTGCTGGTTTGTGTGGGAAAGTCACCCGCCTTAATCACTCAAAACTACTCTTCg                                                                                                                                                                   >  2:1105806/1‑239 (MQ=11)

                                                                             ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCg                                                                                                                                                                   >  1:601501/1‑239 (MQ=14)

                                                                             ccTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAAGGGACACCCCTTGCTTGGTTTTTTGGGAAAGTCACCTGCCTTTATATCTCAAAACTCATTTTCg                                                                                                                                                                   >  1:139999/1‑239 (MQ=11)

                                                                               tGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCggg                                                                                                                                                                 <  1:215707/239‑1 (MQ=31)

                                                                                gctgTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGATGCAGACTGATACGTCCCCTTCGTCTAGGGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGt                                                                                                                                                                <  1:918535/236‑1 (MQ=14)

                                                                                gCGGTTGGATCACCTCCTTACCTTAAAGAAGCGCTCTTTGCAGTGCGCACACAGATTGTCTTATAGAAAGTGAAAAGCATGGCGTCTTGCGAATCAGACTGATGCGTGCCCTTCGTCTAGAGGCCCAGGACAGCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGt                                                                                                                                                                <  2:382184/239‑1 (MQ=11)

                                                                                  ggTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTg                                                                                                                                                               <  1:417639/238‑1 (MQ=31)

                                                                                  ggTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTg                                                                                                                                                               <  1:11577/238‑1 (MQ=31)

                                                                                        aTCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTg                                                                                                                                                               <  1:214772/232‑1 (MQ=31)

                                                                                         tCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTga                                                                                                                                                       <  1:954896/239‑1 (MQ=31)

                                                                                              tcctTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAAACCCCTAGGGGACGCCACTTGCTGGTGTGTGGGTGAAAGGCACCTGCCTTAATATCTCAAAACTCATCTTTGGGGGAGGTTTggagtgt                                                                                                                                                  >  2:116390/1‑233 (MQ=11)

                                                                                                 ttaccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTg                                                                                                                                               <  1:1143198/239‑1 (MQ=31)

                                                                                                  taccttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGGCGCCCCTTCCTGGTTTGTGTGTGAAAGACACACGCCTTATTATCTCAAAACTCATCTTCGGGGGATGttttgttgtcttct                                                                                                                                              >  2:214772/1‑228 (MQ=11)

                                                                                                   accttaAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGGCGCCACTTGCTGGTTTGTGAGTGAAAATCACCTGCCTTAATATCTCAAAACTCATATTCGGGTGATGTTTGAGATATTTGct                                                                                                                                             >  1:416868/1‑239 (MQ=9)

                                                                                                       taAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGACGCCACTTGCTGGGTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGGGATGTTTTAGATATTTGCTCttt                                                                                                                                         >  1:75887/1‑239 (MQ=9)

                                                                                                         aagaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTaa                                                                                                                                       >  1:1066023/1‑239 (MQ=31)

                                                                                                          agaagCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAAACCCCTAGGGGGCGCCACTTGCTGGGTTGTGAGGGAAAGTCACCCGCCTTTATATCTCAAAACTTATCTTCTGGTGGTGTTTGGGATATTTGCTCTTTaaa                                                                                                                                      >  2:717536/1‑239 (MQ=11)

                                                                                                               cGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGGCGCCCCTTGCTGGTTTTTGAGGGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTTTTCTTTTAAAATCt                                                                                                                                 >  1:455401/1‑239 (MQ=11)

                                                                                                                           tGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTTCTGGGTTGTGAGAGAAAGTCACCTGCCTTAATATCTCAAAAATCATCTTCGGGTGTTGTTTTAGATATTTGATCTTTTAAAATCTGGATCAAGCTGa                                                                                                                     >  1:616645/1‑239 (MQ=11)

                                                                                                                           tGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGGTGTTTGAGATATTTGCTCTTTTAAAATTTGGATTAAACTGa                                                                                                                     >  1:744086/1‑239 (MQ=9)

                                                                                                                            gCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑Ga                                                                                                                                                                                                                                            >  1:112752/1‑119 (MQ=32)

                                                                                                                            gCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGaa                                                                                                                    <  1:608844/239‑1 (MQ=31)

                                                                                                                            gCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGaa                                                                                                                    <  1:752565/239‑1 (MQ=31)

                                                                                                                             cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCCCTTGCTGGTTTGTGAGTGAAAGGCACCTGCCTTAATATCTCAAAAATCATCTTCGGGGGATGTTTTAGATATTTGCTCTTTTAAAATCTGGATCAAGCTGaaa                                                                                                                   >  1:343577/1‑239 (MQ=9)

                                                                                                                             cTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGGCACCTGCCTTAATATCTCCAAACTCATCTTCGGGTGATGTTTTTGATATTTTCTCTTTTAAAATCTTGTTCAAGCTGaaa                                                                                                                   >  1:325699/1‑239 (MQ=11)

                                                                                                                              tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGaaaa                                                                                                                  >  1:1111742/1‑239 (MQ=31)

                                                                                                                                 cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTg                                                                                                               <  1:515630/239‑1 (MQ=31)

                                                                                                                                 cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTg                                                                                                               <  1:344730/239‑1 (MQ=31)

                                                                                                                                 cacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTg                                                                                                               <  1:264438/239‑1 (MQ=31)

                                                                                                                                 cacaGATTGTCTGAGAGAAAGGGAAAAGCGAGCCTCCTTGCTAAGCAGACTGATCCGTCCACTTCGTCTAGAGGCCCAGGACACCGCCGTTGCACGGAGGT‑AACAG‑GGCGAC‑GAATCCCCTAGGGGACTCCGCTTGCTGGTTTGTGAGTGAAAGTCAACTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGATATAGTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTg                                                                                                               <  2:297410/239‑1 (MQ=11)

                                                                                                                                  acaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTg                                                                                                                                               <  1:631613/206‑1 (MQ=31)

                                                                                                                                     gATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGACGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AAAAG‑GGGTTC‑GCAACCCCTAGGGGACGCCACTTTCTGGTTTGTGAGTGAAAGTCACCCGCCCTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGTTCTTTTAAAAACTTGATCAAGCTGAAAATTGaaaa                                                                                                           >  2:764208/1‑238 (MQ=11)

                                                                                                                                       ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAaca                                                                                                          <  2:866060/238‑1 (MQ=31)

                                                                                                                                       ttGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAaca                                                                                                          <  2:183682/238‑1 (MQ=31)

                                                                                                                                          tCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACCA‑GGGGTC‑GAATCCCCTAGGGGACGCCACTTGCGGGTTTTTGAGGGAAAATCACCTGCCTTATTATCTCCAAACTCATCTTCGGGGGATGTTTTGGATATTTTCTCTTTTAAAATCTGGATAAAACTGAAAATTTAAACACTGa                                                                                                      >  2:944899/1‑239 (MQ=11)

                                                                                                                                           cTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGa                                                                                                      <  1:137137/238‑1 (MQ=31)

                                                                                                                                              aTAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTT‑GAATCCCCTAGGGGACGCCACCTGCTGGTTTGTGAGGGAAAGGCACCTGCCTTAATATTTCAAAAATCATCTTTGGGGGATGTTTGGGATATTTGCTCTTTAAAAATCTGGGTCAAGCTTGAAATTGAAAAAATGaacaa                                                                                                  >  1:164860/1‑239 (MQ=11)

                                                                                                                                              aTAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGC‑AACAA‑GGGCGC‑GAAACCCCTATGGGGAGCCCCTTGCTGGGTTGTGAGTGAAAGGCACCCGCCTTAAATTTTCCAAAATCAGCTTCTGGGGTTGGTTGGGATATTTTCTCTTTAAAAATCTTGATCAAACTGAAACTTTGAACACTGaaaaa                                                                                                  >  2:603651/1‑239 (MQ=11)

                                                                                                                                               tAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGGGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGaacaac                                                                                                 <  1:694246/239‑1 (MQ=14)

                                                                                                                                                                                                 cGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GGATCCCCTCGGGGGCGCCACTTGCTGGTTTGTGAGGGAAAGGAACCTGCCTTTATATCTCAAAACTCATCTTCGGGTGGTGTTTTAGATATTTGCTCTTTAAAAATCTGGGTCAAGCTGAAAATTTAAAAACTGAACAACGAAAGTTGTTCGTGAGTCTCCCCAATTTTCGCAACCCGATGATGAATCGt                                               >  2:574832/1‑239 (MQ=11)

                                                                                                                                                                                                 cGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGGGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTTAAACACTGAACAACGGAAGTTGTTCGTGAGTCTCTCAAATTTTTGCCAAACGATGATGAATCGt                                               >  1:1069572/1‑239 (MQ=2)

                                                                                                                                                                                                        agGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTTTGAGTGAAAGTCACCTGCCTTTATATCTCCAAACTCATCTTCGGGGGATGTTTGTGATATTTTCTCTTTAAAAATCTTGATCAAGCTGCAAATTTAAAAACTGAACAACGGAAATTGTTCGTTAGTCTCTCAAATTTTCGCAACACGATGATGAAACGTAAGaaaa                                        >  2:586433/1‑238 (MQ=11)

                                                                                                                                                                                                         ggCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAAGCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGaaa                                         <  2:17118/237‑1 (MQ=14)

                                                                                                                                                                                                            ccAGGACGCCGCCCTTTCACGGCGGT‑AACAG‑CGGGAC‑GAATCGCCTAGGCGGCGGCGCTCGCTGGTTCGTTAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATAGTTGCTCGTTAAAAATCTGGAGCAAGCTGAAAATGGAAACACTGAACAACGAAAGTTGTACGTGAGTCTCTCAAAGTGTCGCAACACGATGATGAATCGTAAGAAACATCt                                    <  2:416868/239‑1 (MQ=11)

                                                                                                                                                                                                            ccAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTCGGGGACGCCACCTGGTGGTTTGTGAGTGAAAGTGAACTGCCTTAATATCTCAAAACTCATCTTTGGCTGATGTTTGAGCTATTTTCTCTTTAAAAATCTGGAATTAGCTGAAAATTGAAACACTGTACAACGAAAAATGTTCGTGAGACTCTCAAATTTTTGCAACACGCTGATGAATTGTAATAAACATCt                                    >  1:897673/1‑239 (MQ=11)

                                                                                                                                                                                                             cAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCtt                                   <  1:586433/239‑1 (MQ=31)

                                                                                                                                                                                                             cAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAATCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAATCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCt                                    >  1:100124/1‑238 (MQ=14)

                                                                                                                                                                                                             cAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGGTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGGGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCt                                    >  1:52933/1‑238 (MQ=14)

                                                                                                                                                                                                                 accccGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑g                                <  1:60368/234‑1 (MQ=14)

                                                                                                                                                                                                                     cGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTtgt                          <  1:603651/239‑1 (MQ=31)

                                                                                                                                                                                                                      gCCCTTTCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGGCGCCACCTGCTGGGTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGGGGTGTTTTGGATATTTGCTCTTTTAAAATCTGGATCAAGCTGAAAATTGAAAAACTGAACAACGGAAGTTGTTCGTGAGTCTTCCACATTTTTGTAACACAATGATGAATCGTAAGAAAAATCTTC‑GGGAtgtg                         >  2:197985/1‑239 (MQ=11)

                                                                                                                                                                                                                        ccactCACGGCGGT‑AACAG‑GGATTC‑GAATCCCCTAGGGGACGACGCTTGCTTGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAATGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGa                        <  2:164860/234‑1 (MQ=9)

                                                                                                                                                                                                                          tttCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCCCTTGCTGGTTTTTGAGGGAAAATCACCTGCCTTTATATTTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTTAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAATTGTTTGTGAGTCTTTCAAAATTTTCCAAAACGAATATTAAACGTAAGAAACATCTTCGGGGTTGTgggg                      >  2:609253/1‑239 (MQ=11)

                                                                                                                                                                                                                          tttCACGGCGGT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGGCACCTGCCTTAATATCTCAAAACTCATCTTCGGGGGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAATTTTTCGTGAGTCTCTCAAATTTTCGCAACCCGATGATGAATCGTAAAAAACATCTTC‑CGGATGTGAGGt                     >  1:308195/1‑239 (MQ=11)

                                                                                                                                                                                                                           ttaaCGGCTGT‑AACAG‑GGGTTC‑GAATCCCCTAGGAGACGCCTCTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGtt                    <  2:522179/236‑1 (MQ=9)

                                                                                                                                                                                                                                  cggT‑AACAG‑GGGGTC‑GAATCCCCTAGGGGACGCCCCTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTTCGATATTTGCTCTTTAAAAATCTGGATCAAACTGAAAATTGGAACACTGAACAACGAAAGGTGTTCGGGAGTTTCTCAAATTTTCTCCACACGATGATGAATCGTAAGAAACATCTTT‑GGGTTGTGAGGTTAAACg               >  2:399312/1‑237 (MQ=11)

                                                                                                                                                                                                                                     t‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGTTAAGCGACTa           <  1:574832/238‑1 (MQ=31)

                                                                                                                                                                                                                                             ggtttC‑GAATCCCCTAAGGGATGCCACTTGGTGGTTGGTGAGTGAAAGGAACGTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTATTTAAAAATCTGTATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAAATTTCGCAACACGATGATGAATCGTAAGAAGCATCGTC‑GGGTTGTGAGGTTAAGCGACTAAGCGTa     <  2:343577/235‑1 (MQ=11)

                                                                                                                                                                                                                                              ggTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGTTAAGCGACTAAGCGTa     <  2:596896/237‑1 (MQ=31)

                                                                                                                                                                                                                                               gTTC‑GAATCCCCTAGGGGACGGCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGTTAAGCGACTAAGCGTaca   <  2:325699/238‑1 (MQ=14)

                                                                                                                                                                                                                                               gTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGTTAAGCGACTAAGCGTaca   <  1:420907/238‑1 (MQ=31)

                                                                                                                                                                                                                                                ttC‑AAATACCCAAGTGGACGGCACTTGCTGGGTTGTGAGTGAAAGTCAGCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTAAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGTTAAGCGACTAAGCGTacac  <  2:1054486/238‑1 (MQ=11)

CTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGT‑AACAG‑GGGTTC‑GAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCACCTGCCTTAATATCTCAAAACTCATCTTCGGGTGATGTTTGAGATATTTGCTCTTTAAAAATCTGGATCAAGCTGAAAATTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGTAAGAAACATCTTC‑GGGTTGTGAGGTTAAGCGACTAAGCGTACAC  >  NC_000913/4209589‑4210064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`