| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 989,943 | T→G | 22.9% | S138S (TCA→TCC) | pncB ← | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 989,943 | 0 | T | G | 22.9% | 149.2 / 5.1 | 79 | S138S (TCA→TCC) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (20/37); new base G (12/10); total (32/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATC > NC_000913/989707‑990177 | gtTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTg < 1:52679/238‑1 (MQ=255)gtTCCCAGCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTg < 2:1099742/238‑1 (MQ=255) ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATTGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcga < 2:110450/239‑1 (MQ=255) ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCTCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcga < 1:950384/239‑1 (MQ=255) ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcga < 1:611882/239‑1 (MQ=255) ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcga < 1:529084/239‑1 (MQ=255) ccATCCGCTGGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTTCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcga < 2:961014/239‑1 (MQ=255) cATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcgat < 2:635802/239‑1 (MQ=255) tCGGCGTGAGGGAAAGCCGACGCACCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcgatag < 1:249339/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcgatagcg < 1:1075988/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcgatagcg < 2:211184/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcgatagcg < 2:1220634/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGagcgatagcg < 2:1023546/239‑1 (MQ=255) cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTTACGCCGAGAAAGTGACCAATTTTCTTTCCAACGGGTC‑GGGGGCGTTGGC‑AAACTC‑GGCCTGCGGTGagcggtagcgat > 1:414676/1‑239 (MQ=255) cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGCTAACACCCAGAAGACGAGTAAATTGCTTTTCAGCGCGGA‑GGGGGCGTGCGC‑ACCGCC‑GCGCCGCCGGGatcggtagcgag > 2:548900/1‑238 (MQ=255) gTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATg < 1:182974/239‑1 (MQ=255) gggAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTAc < 1:992390/238‑1 (MQ=255) aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACCAATTTGCTTTCCAGCGTGTCGGAGGGCTGGGCC‑AAC‑TC‑GGCCTGCGGTGAGCGCTAGCGCTGTACCActt > 2:897698/1‑238 (MQ=255) aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTAGACTAATTTGCTTTCCAGCGTGTC‑GAGGGGTTGCGC‑CAAGTC‑GGCCTGCGGGGAGCGAGAGCGATGTAcccattt > 1:148373/1‑234 (MQ=255) tCGTATTTGCTGGTGCCCACAAACCAGGATTCCTGTGGCAGA‑CGTTTAACTATGGTTTCTTTTACTACGAGAAAAAAGCGGCGACGGGTGCCAAAACCCATCAG‑ATGGAAGCGCGACATATCAAGCCCGGGGGTTAACGCCGAGAGGGCGACTAATTTGCTTCCCAGCGTGTC‑GAGGGTTGGCGC‑AACGCC‑GGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCG‑cc < 2:270343/237‑1 (MQ=255) gcggtggCCCACAAACCAGGATTCCGGTTGCAGA‑CGTTTAACGATGTTTTCTTTTACTTCGCGAAAAAAAGGGCGACGGGTCCCAAAATCCACCAG‑ATGGAAGCCCGACATACAAAGCCCGGCGTTTAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGaa < 2:148373/233‑1 (MQ=255) cTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCCGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGGGATGGACCATTTTACCGATAACCG‑CCAGCAAAAGa > 1:1209956/1‑237 (MQ=255) tGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GGGGGCTTGCGC‑CACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTTACTGATAACCC‑CCCGCACAAGAACt > 2:428772/1‑239 (MQ=255) ggTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAAATTGCTTTCCAGCGTGTC‑GGGGGGGTGCGC‑AAACTC‑GGCCTGCGGGGGGCGATAGCGCTGTCGCAATTTACTGATAAACGCCCACCAAGGGAACt > 2:44251/1‑239 (MQ=255) gCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGGACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTTACGCCGACAAATCGACTAATTTTCTTTCCCGCCTGGT‑GAGGGCTTAAGC‑AAAGTC‑CGCCTGCGGTGAGCGAAAAGGGTTGACCCTTTTCCTGAGACACG‑CCAGCAAAGGGAATTcgc > 2:649585/1‑237 (MQ=255) gCCCACAAACCAGGATTCCTGTGGCAGA‑CGCTTAACGATGTTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAAGCCATCAG‑ATGGAAGCGCGACATATCAAGCCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTcc < 1:1212552/238‑1 (MQ=255) caAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTTCAACGGGGC‑GAGGGCCTGCGC‑AACGCC‑GGCCCGCGGTGAGCGATAACGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCCGAgg > 1:136552/1‑239 (MQ=255) aaCCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCAAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTACCGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGAt < 1:428772/239‑1 (MQ=255) aCCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAAGTGACTAATTTGCTTTTCAACCGGTC‑GAGGGGCTGCGCAAACGTG‑GGCC‑GCGGGGGGTGGAAGAGGTGTACCATTTAACTGATAACCC‑CCAGGAAAGAAACATCCCAGAGGaag > 2:934169/1‑237 (MQ=255) gATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTTCAACGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGGCCGCGGGGGGCGATAGCGGTTTACCATTTCACCGATAAACG‑CCCGCAAAGGAAATTTCCCGAGGGTGACtt > 2:519363/1‑238 (MQ=255) aTTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTc < 1:484931/238‑1 (MQ=255) tttccgTTGCAGGCCGCTTAAGGATGGTTTCTTCTACGTCGCGAAAAAACCGGCGACGGGTGCAAAAAGCCATAAG‑ATGGAAGGCCGAAATATCAAGACCGGGGTTTAACGCCGAAAAGTCGATTAATTTGTTTCCCAGCGTGTC‑GAGGGCTTGCGC‑ACCGTC‑GGCCTGCGGTGGGCGATAGCGATGTCCCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTc < 2:784158/233‑1 (MQ=255) cTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTTCTTTTCAGCGGGTC‑GAGGGCTTGCGC‑AACGGC‑GGCCTGCGGTGAGCGGTAACGATGTAACATTTTACTTATAAACG‑CCCGCAAAGGAACTTCCCCGAGGGTGACTTTACGc > 1:532900/1‑238 (MQ=255) cTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCCAGAAGTCGACTCATTTTCTTTCCCGCGGGTC‑GGGGGGTTGCGC‑AACGCC‑CGCCCGCGGGGGGGGGTAGCGGTGTACCATTTTCATGATAAACC‑CCCGCAAAAGGACATCCCAGGGGGAGACtttccgc > 2:182688/1‑233 (MQ=255) ttttCTTGTACTGCGGAAGAAAAAGGGCGAGGGGTGCCAAAATCCA‑CAGAAGGGAAGCGCGACATATCAAGACGGGCGTTTAACGCGGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGA‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAatat < 1:967773/237‑1 (MQ=255) tGTCCTTGGGGAAAAAAGCGGGGACGGGGCCAAAAACCCATCAG‑ATGGAAGCCGGACATATCAAGCCCGGCGGTTAACGCGGAGAAGTCGACTAATTGGCTTTCCAGCGTGTC‑GAGGGCTTGCGA‑ACCGTC‑GGCCTGCGGGGAGCGATAGCGATGTCCCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCtt < 2:961553/239‑1 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGGGTC‑GGGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCATTTCAATGATAACCG‑CCCGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGc > 1:1235500/1‑210 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCCGCGGTGGGCGATAGCGATGTAACATTTCACTGCTAAACG‑CCAGCAAAAGAACTTCCCCCAGGATGACTTCACGCCACCGGCCGCTTAAACGCATATCCAACTTTCCCttt > 1:1001106/1‑238 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGGACAACAAATTGCTTTACAGAGGGTCGGAGGGC‑TGCGC‑GAAATC‑GGGCTTCAGGGGGCGATAGACATTTCACATTTCCTCGCAAAACG‑CCCGCCAAGGGACTTACCAGAAGAAGGGTTCCAGCCACCGGGCGCCTAAACCGATATTCCACTTGCCACTa > 2:210005/1‑239 (MQ=255) aaccGGCGAGGGGGGCAAAAACCCATCAG‑ATGGAAGCGCGACATACCAAGACCGGCGGTAAACGCCGAGAAGTCGACTAATTTGCTTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGGGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:296234/236‑1 (MQ=255) gcaaaaacacATCAG‑ATGGAAGCGCGACATATCAAGCCCGGCGTTTAACGCCGAGAAGTCGACTAATTTGCTTCCCGGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCGGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 2:532900/230‑1 (MQ=255) gacAAAACCCATAAA‑GTGGAAGGCCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTCTTTTCCAGCGTGTC‑GGGGGTTGGCGC‑ACCGCC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCCCTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 2:849192/237‑1 (MQ=255) ccAAAATCCATCAG‑AGAAAAGCGGAAAATATAAGGACCGGCTGTTAACGCGAATAAGTCGACTATTTGGCTTCCCGGCGTGTG‑TGGGGCTTGCGC‑ACCGTG‑CGCCGGCGGTGGTCGAGAGCGAGTTCCCATTTCACTGATACCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 2:943213/238‑1 (MQ=255) ccAAAACCCATCAG‑ATGGAAGCGCGACATATCAAGGCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 1:519363/238‑1 (MQ=255) tCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCCTTTCACTGATAACCG‑CCCGCCAAGGAACTTCCCAAGAGATGACTTCACGCCAACGGCCGCTTAAACGAAAATCCAGCTTGCCATTATCATTGGACACAGGGGCTTG‑TTCCGGGGTaaa > 2:572585/1‑238 (MQ=255) aTGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTg < 2:950375/98‑1 (MQ=255) ggAAGCGCGAAAGATCAAGACCGGGGGTTAACGCCGAGAAGCCGACTAATTTGCTTCCCAGCGTGTC‑GAGGTCTTGCCC‑AACGTC‑GGCCGGGGGTGAGCAATAGCGATGTACCTTTCCCCTGAAAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGaa < 2:1209956/238‑1 (MQ=255) aaCATATCAAGCCCGGGGGTTAACGCCGGGAGGTCGACTATTTTGTTTCCCAGCGTGTC‑GGGGGTTTGCGC‑AACGGC‑GGCCTGCGGGGGGCGATAGCGATGTCCCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTaa < 2:289057/238‑1 (MQ=255) aCATATCAAGCCCGGCGGTTAACGCGGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCCCTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTaa < 1:44251/238‑1 (MQ=255) tatCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAAATTTCTTTCCACCGTGTC‑GAGGGCTTGCGC‑CACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCCTTTCACTGATAACCG‑CCCGCAAACGCACTTCCCCGAGGATGACTTCCCCCCACCGGCCGCTTAAACGAATATCCCGCCTGCCCTTATTCTTGTACACGGTGGCTTGTTTCCGGGTTAAA‑CGGACAACGCGGAACCac > 2:689178/1‑238 (MQ=255) cAAGCCCGGGGTTTAACGTCGAAACGTCGGCTAGTTGGCTTCCCAGGGTGTG‑GAGGGTTTGCCC‑AAGGTC‑GGCGCGGGGGGACGGATAGCGTGGTCCCATTTCTCGAATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa < 2:1226803/239‑1 (MQ=255) gggggTAAACGCCGGGAAGGCGAATAATTTGCTTTCCAGCTGGTC‑GAGGGCATGCCA‑ACCGTC‑GGCTGGCGGTGACGAATAGCGATGTACAATTGCCCTGAGAACCG‑CCAGCAAAGGAACTGCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCGTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 2:157560/236‑1 (MQ=255) gcggTTTAAGCCGAGAAGTCGACTAATTTGCTTTCCAGCGGGTC‑GGGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCCTTTTACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCCCGGGCCGCTTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGGGGATTG‑TTCCGGGGTAAAGCGGAACTCCGGGAAACAGTTaaaaaaaac > 2:686493/1‑232 (MQ=255) gcggTTAACGCCGAGAAGTCGACTAATTTTCTTTCCAGCGTGTC‑GGGGGCTTGCGC‑AACGGC‑GGCCTGCGGGGAGCGAGAGCGGTGTACCATTTTACTGATAAACG‑CCCGCCAAGGAACTTCCCCGAGGGTGACTTTCCCCCACCGGCCGCTTAAACGAAAATACACCTTGCCCTTATCGTTTGACACGGTGACCTG‑TTTCGGGTTCAAGCGGAACTCCCGTAAACCGTTACGATAGt > 2:631283/1‑238 (MQ=255) cggTTACCGCCGAGAAGTCGACTAATTTGCTTCCCAGGGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGAGATTTCCCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc < 1:740722/238‑1 (MQ=255) cggTTAACGCCGAGAAGTCGACTAATTTGCTTTCCGGCGTTGC‑GTGTGCTTGCGA‑ACCGGC‑TGCGTGCGGGGATCGATAGCGATGTCCCATTTCCCTAAAAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc < 2:136552/238‑1 (MQ=255) agaAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaa > 2:379034/1‑239 (MQ=255) agaAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaa > 2:116645/1‑239 (MQ=255) aaGTCGACTAATTGGCTTTCCAGCGTGTC‑GGGGGCTTGCCA‑ACCGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg < 1:901034/239‑1 (MQ=255) agtcatagaaTTTGTTTCCCACTGTGCC‑GAGGGTTTGCGC‑CCCGTG‑GGCCTGCGGGGAGAGTTAGTGATGTACCATTGCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg < 1:1158402/230‑1 (MQ=255) gTCGGCTAATTTGCTTTCGTTGGTGGG‑GAGGTCTTGCGC‑ATGGCC‑GCCCTGCGTGGAGGGAGAGCGATGTCCTTTCTCATTGATACCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggc < 2:379445/238‑1 (MQ=255) tCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcag > 2:1218794/1‑239 (MQ=255) aCTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcagggc > 1:204556/1‑239 (MQ=255) tgcttccgggCGTGTG‑GGGGGCTTGCG‑‑AACGGCGGGCGAGCGGTGGGGGATGGCGGTGTTCCATTTCGCTAATACCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc < 2:20286/230‑1 (MQ=255) ttCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGcc > 1:707889/1‑236 (MQ=255) cAGTGTGTC‑GGGGGTTTCGGC‑AATCGC‑GGCCTGCGTGGGCCAATAGCTATGTACCATTTCACTTATATCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGat < 1:865714/239‑1 (MQ=255) gtgtC‑GAGGGTTTGCGC‑AACGCC‑GGCCGGCGGGGAGCGATAGCGATGTACAATTTCACAGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATAtt < 1:416752/238‑1 (MQ=255) gtggC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCCCTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATAtt < 1:1218794/238‑1 (MQ=255) tgtC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtca > 1:819315/1‑239 (MQ=255) tGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg > 1:731040/1‑239 (MQ=255) gcgc‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg > 1:1091819/1‑238 (MQ=255) gcgc‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg > 2:249316/1‑238 (MQ=255) gCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgct > 2:334793/1‑238 (MQ=255) cggCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:1152536/237‑1 (MQ=255) cTGGGGTGAGCGATACCGTTGTGCCATTTCACTAATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:680435/239‑1 (MQ=255) cTGCGGTGGGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:182688/239‑1 (MQ=255) cTGCGGTGAGGGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgctgc < 1:1117046/238‑1 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc > 2:678394/1‑238 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc > 1:467246/1‑238 (MQ=255) | GTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATC > NC_000913/989707‑990177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |