New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3220237 =99 (0.770)4 (0.030) 4/408 NT 3.3% coding (744/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
?NC_000913 3220409 = 138 (1.130)coding (916/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Rejected: Frequency below/above cutoff threshold.

GGTATGCAGGAATGGGTTGTCGAACAGAACTGAAAACACCTCTTCAGTGGCGATGGTCGCGCCAATCGGCATCACGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3220483‑3220237
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcggcggcGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAA  >  NC_000913/3220409‑3220642
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
GGTATGCAGGAATGGGTTGTCGAACAGAACTGAAAACACCTCTTCAGTGGCGATGGTCGCGCCAATCGGCATCACGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCG                                                                                                                                                                                                                                                    >  2:650832/1‑239
    TGCAGGAATGGGTTGTCGAACAGAACTGAAAACACCTCTTCAGTGGCGATGGTCGCGCCAATCGGCATCACGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGG                                                                                                                                                                                                                                                <  1:1275833/239‑1
                        catAACTGAAAACACCTCTTCAGTGGCGATGGTCGCGCCAATCGGCATCACGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCGTGATGCCGATTGGCG                                                                                                                                                                                                                            <  1:921361/236‑1
                                                                                                         GATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCG                                                                                                                                            >  2:481155/1‑238
                                                                                                                GGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTG                                                                                                                                    <  1:481155/239‑1
                                                                                                                                                                    CCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCT                                                                                <  1:36993/239‑1
                                                                                                                                                                                                                                             CGGCGGCGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGGGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCCAAAAGGCGCTATGTTTCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCGCGATCTGGTAC       >  2:230570/1‑239
                                                                                                                                                                                                                                                  ggGGCGGGATGCCGATTGGAGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCAGTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGA   <  2:578849/236‑1
                                                                                                                                                                                                                                                   CGGCGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAAATGGCGCGGGAATATCCCGATCTGGTACAGGAA  >  1:1382955/1‑238
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
GGTATGCAGGAATGGGTTGTCGAACAGAACTGAAAACACCTCTTCAGTGGCGATGGTCGCGCCAATCGGCATCACGCCGCCGCCGAGCGCTTTGGCAAGGCAGAGGATATCCGGCTGTACGTTCTCATGCTCGCAGGCGAACATCTTGCCCGTGCGCCCCATGCCCGTTTGTACTTCATCGAGGATCATCAGTGCGCCGAACTCATCGCATAGCTTACGTACGGCGGTGAGATAGCCCGGCGGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3220483‑3220237
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggcggcggcGTGATGCCGATTGGCGCGACCATCGCCACTGAAGAGGTGTTTTCAGTTCTGTTCGACAACCCATTCCTGCATACCACCACCTTTGGCGGCAACCCGCTGGCCTGTGCGGCGGCGCTGGCGACCATCAATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAA  >  NC_000913/3220409‑3220642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.