| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,151,689 | G→T | 31.5% | intergenic (+43/+22) | yegJ → / ← yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,151,689 | 0 | G | T | 31.5% | 24.8 / 5.3 | 34 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (12/11); new base T (5/6); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01 | |||||||||||
CCCTGGTATTGTTACAAACGTAGAATATGGCCA‑GGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCAT‑TGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT > NC_000913/2151469‑2151924 | ccttGGTATTGTTAAAAACGAAGAATATGGCCA‑GGAGTGAAAAAAAAAAAAAGAAGATATTACAGACTGGATGTATACGGGGGGGGAAAAAATTTACGGCGGTTATCCCAT‑TGA‑TCTTTTATTGGTCACCTACCAAAAAGAAGAAGCCGACGAGTTAAGAGAAAAATTAGTCCGCTAATCAGCCGTTATATTTATTGTTAAAAC‑GGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATgaga < 1:290155/236‑1 (MQ=255) aacgaaaaataggCCAGGGAGTGAAAAAAAAAAAAAAAAAATATTTCAGACTGGATTTATACGCGGGGGAAAAAAATTTACGGCGGTTATACATT‑TGA‑TCTTTTATTGGTCACCTACCAAAAAGAAGAAGCCGCCGAGCAAAGAGAAAATTAATTTCGCAAATCAGCCGTTATATTTATTGTTAAAAC‑TGGGGGTTG‑ATTTG‑TTTTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCcac < 1:60891/228‑1 (MQ=255) gatGGAAAATCAATAATGAAGAGATGTCAGACTTGGCTTATTCGTGCGTGCAGAAAATTTAC‑GCCATT‑TACCATATGATTTCTTTATTGTTCCCATATCAAAAAGAACAAGCGATCGAGTAAAGAGCAAAATAACTTATCGACTGATCGGATATATTTTTTTTTGAACT‑TGGGGGTTG‑AATTG‑TTGTCTAT‑TT‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt < 2:1033012/236‑1 (MQ=255) atggaaagagcAATAAAAATGATATTTCAGCCTAGAATTGTACGTGTGAGAAAAAATTTGAGGCCGCTTATCACAT‑TGA‑TCGTTTATTGGAAACCTAGCAAAAAAAAGAATCCGGGCAGCAAAAAACAAATCAAGTTCGATAACGGGCTGTAATATTTTTGGTTAAACC‑TGGGGTTTA‑ATTTG‑TTGTCTGT‑GA‑CCTCCATGAGAAACTCGCTCTGCCCACAGCGCCAGTGCCAACGtt < 2:664192/228‑1 (MQ=255) tatgCGGGCGGAGAAAAAATGTACGGGTGTTATACCTT‑TAT‑TCATTATTTGGTCCCCTACAAAAAAAAAGAACCCGAGAATGAAAAAAAAAATTTGGTCCGTAAATCGGCTGTTATTTTTTTTTTTTAACC‑TGGCGGTGA‑AATGT‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑c < 2:101058/239‑1 (MQ=255) cAT‑TGA‑TCTTTTATTGTCCCCCTAACAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTAGTTCGCTAACCAGCCTTTATATTTATTGTTTAAAC‑GGGCGTTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAg < 1:1236156/239‑1 (MQ=255) aaT‑TTA‑TCTTTTATGGGCCCCATATCAAAAAAAAGAAGCCGACGATAAAAGAAAAAATGTAGTTCGCTAATCGGCCTTTATTTTTATTTTTTAAAC‑TGGGGGTTA‑ATTTT‑TTGTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGtt < 2:94827/164‑1 (MQ=255) tGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTT‑AATTA‑TTGTCTTC‑AA‑ACGCCAATAGAAACTCAATCTGCCCACAGCGCCAGAGCTAACGGTTTCTCATCATACGCACGCTCGTTTTCCCCCGGGCTGG‑C‑CAAAAACTTGTTTTACAAATCAGGTGTTAAATCGCgttg > 2:707072/1‑239 (MQ=255) tGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTTTTTTT‑AA‑ACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATTCTCATCCCTACCCCCCTTTAACGCCGGGCTGG‑A‑CAAAAACTGGCTTACAAATTCAAGGAATaaaacaggttg > 2:646345/1‑231 (MQ=255) tGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATTAGAAAATCACTCTGCCCACAGCGCCAAGTCCCACGCTTTCTCATTATCCATACGCTCCTTTACCCCCCGCCTGG‑G‑CCAAAACTGCTTTTACCAATCAGTTTATAAATCGAgttg > 2:64713/1‑239 (MQ=255) gA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTT‑TTTTCTTC‑AA‑CCGCCCTTAGAAAATCAATCCTCCCACAGCGCCAGTGCCCAAGTTTTATCATCCACCGTACGCTCGTTTACCCCCCGGCTGGAA‑CAAAAACGCTTTTAACATTTC‑CGTAATAAAtaacggtgg > 2:1085913/1‑231 (MQ=255) gA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑ACCCCATTAGAAACTCACTCTTACCACAGCCCCAGTGCCAACGCTTTATCATCAATCCTACGCTCGTTTTCCGCCAGGCTCG‑A‑CAAAAAATGCTTTAACAATTCAGGGAATAAATCCAggtgt > 1:1221147/1‑239 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑GATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTatcatcat > 1:94827/1‑165 (MQ=255) ttGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATTAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGTTTTatc > 1:1439644/1‑157 (MQ=255) gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTT‑TTGTCTTC‑AA‑CCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGAGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCACGTAATAAATCGAGTTGTTCCTGCGtt > 2:1088214/1‑236 (MQ=255) tCACCAAACAAAAAAAAGAGGCCGACGAGCAAAGACAAAAATTAGTTCGTAAACCAGCCTTTATATTT‑TTTTTTAAACGGGGGGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTtgct < 1:646345/238‑1 (MQ=255) gcACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGATA‑AATTT‑TTGTCTTC‑AA‑CCGCCCTGAGAAACTCACTCTTCCCACCGCGCCAGCGCCCACGGTTTATCATAATACGTACGCCCGCTTACCGCCGGGCTGG‑A‑CCAAAACAGCTTTATAAATTCAGGTAATAAATAGAGTTGTTCCTGCGTtgct > 2:1153523/2‑238 (MQ=255) tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTTCCGCCAg > 1:880319/1‑239 (MQ=255) aagaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCCTTAGAAACTCACTCTTCCCACAGCGCCAGTGCCCACGTTTTTTCATCATCCGTACGCTCGTTTACCCCCGGGCTGG‑A‑CAAAAACTGGTTTTACAATTCAGGTAATTAAACGAGTTGTTCCTGCGTTGCTGCCGCCCGTccct > 2:311696/1‑237 (MQ=255) gagcaaagagcaaaATTAGTTCGCTAATCAGCAGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTCC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTa < 1:1003741/239‑1 (MQ=255) aaaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCCTACGCTCGTTTACCGCCCGGGTGG‑G‑CAAAAAATGCTTTAACAAATCAGGTAATAAATCGAGTTGTTCCTGCGCTGCTGCCGCCCGTCCCTTGAAAAACGGGGGTAAGAAAGGcaca > 1:906293/1‑239 (MQ=255) tgcgttAATCGGCTGTTTTATTTTTTTTTTAACC‑GGGGGTTTG‑ATTTG‑TTTTCTTA‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgaggag < 1:1332298/234‑1 (MQ=255) aaTCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTTTCTTC‑AA‑ACGCCCTGAGAAACTCCCTCTGCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGTAACCTCGTTTTCCGCCGGGCCGG‑ACCAAAAACT‑CTTTAAAAATTCAGGGAATAAAATGAGTTGTTCCTGCCGTGCTGCCCCCACTCCCTTTGAAAAAGGGGTAAAGAAAGGCACATGAGGAGAAatat > 1:583168/1‑239 (MQ=255) ccacccttaatttttttttttTAAC‑CGGGGGTTA‑AATTT‑TTTGCCTC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATcc < 1:140721/228‑1 (MQ=255) caattatttatt‑TTTAAAC‑GGGGGGTTA‑AATTT‑TTTTCCTC‑AACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGCACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCa < 1:1432895/233‑1 (MQ=255) atatTTATTGTTTAAAC‑TGGCGGTTT‑AATTG‑TTGTCTTT‑AA‑CCCCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGGTGG‑A‑CAAAAACTACCTTTACAAtttcggg > 1:1413158/1‑144 (MQ=255) tttattGTTTAAAC‑TGGCGGTTG‑AATTG‑TTTTTTTC‑AA‑CCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCCGGGTGG‑A‑CAAAAACTGCTTTTACAATTCCGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCCTTGGAAAAAGGGGGAAAGAAAAGCACATGAGGAGAAATATTCAGCCTATTTAAc > 2:1420757/1‑239 (MQ=255) ttttAAAC‑GGGGGGTTGAAATTT‑TTTTCACC‑AC‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg < 1:752739/238‑1 (MQ=255) ttttAAAC‑AGGCGGTTG‑AATTA‑TTGTCTAC‑AAGCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATACAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg < 1:568031/238‑1 (MQ=255) tgtaaaaC‑GGGGGTTTG‑ATTTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGt < 1:1028394/235‑1 (MQ=255) tttAAAC‑TGGGGGTTG‑AATTT‑TTTTTTTCAAA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg < 1:1308690/238‑1 (MQ=255) tAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAAAGTTTTATCATCATCCGTACCCTCCTTTACCGCCCGGCCGG‑A‑CAAAAAATTCTTTTACAATTCAGGGAATAAATCGAGTTGTTCCTGCGGTGCTTCCGCCCGTCCATTGGAAAAAGGGGGAAAAGAAGGCACATTAGGAGAATTATCCAGCCTATTTAAAGCCAGTCg > 1:925522/1‑238 (MQ=255) ggCGGTTG‑AATTG‑TTTTCTTC‑AA‑ACGCCCTGAGAAACTCACTCTGCCCACAACGCCCGGGCCCACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTTAGGTAATAAATCGAGTTGTTCCTGCGTTGCTTCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACCTGAGGAGGATTATCCAGCCTATTTAACGCCCGTCGCTGGAt > 2:936614/1‑238 (MQ=255) ggTTT‑AATTGTTTGTCTAA‑AC‑CCGCCATGAGAAACTCCCTCTGCCCCCAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc < 1:1040223/238‑1 (MQ=255) ttgtCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt > 1:198900/1‑238 (MQ=255) gtCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt > 1:441151/1‑236 (MQ=255) | CCCTGGTATTGTTACAAACGTAGAATATGGCCA‑GGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCAT‑TGA‑TCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAAC‑TGGCGGTTG‑AATTG‑TTGTCTTC‑AA‑CCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGG‑A‑CAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT > NC_000913/2151469‑2151924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |