BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,471	C→G	13.3%	S3R (AGC→AGG)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,471	0	C	G	13.3%	213.1 / 7.8	108	S3R (AGC→AGG)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base C (22/61); new base G (12/2); total (38/70)
Fisher's exact test for biased strand distribution p-value = 4.04e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 112 total aligned reads displayed.

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206236‑3206709

tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGTCGCAATATTCATTCTTATTGTCGCGTTGATTCACGAACTGGTAATGACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGa                                                                                                                                                                                                                                                                  <  2:55961/238‑1 (MQ=255)

          cTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGaaa                                                                                                                                                                                                                                                                <  1:288314/230‑1 (MQ=255)

             ttCAGCATCGGTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAGT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑c                                                                                                                                                                                                                                                <  2:927167/239‑1 (MQ=255)

              tCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑a                                                                                                                                                                                                                                              <  1:1433478/239‑1 (MQ=255)

                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                                   <  1:588961/236‑1 (MQ=255)

                   aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                                   <  2:597756/236‑1 (MQ=255)

                    tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATAAAAATAC‑AAAAAAAGGAGTTTGCAT‑GATGAGGGAAAGAAATAAGAAA‑‑‑‑A‑A‑‑‑gtctct                                                                                                                                                                                                                                     >  2:288314/1‑234 (MQ=255)

                    tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATTGAGTTGGCAT‑GATGAGCGGAAAGAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GGA‑Gt                                                                                                                                                                                                                              >  1:398496/1‑239 (MQ=255)

                     cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑Gc                                                                                                                                                                                                                                  <  1:844009/235‑1 (MQ=255)

                     cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTg                                                                                                                                                                                                                             <  1:1290429/239‑1 (MQ=255)

                       ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTTGGATTGACAC‑TAGGAGGGAAAGTAATAAAAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑ATGaa                                                                                                                                                                                                                           >  2:601997/1‑239 (MQ=255)

                         aaTTATCCGCAGGTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATa                                                                                                                                                                                                                         <  2:1382686/239‑1 (MQ=255)

                             aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑c                                                                                                                                                                                                                                                <  1:1084271/223‑1 (MQ=255)

                              tCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTg                                                                                                                                                                                                                    <  1:1083770/239‑1 (MQ=255)

                                             aGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATAA‑AAAAAAATGAGTTGTCATGGTTGAG‑GGAAGTAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGAAAAAAGTG‑ACAGAGATGTTCGc                                                                                                                                                                                                     >  2:894581/1‑238 (MQ=255)

                                             aGCGAAGGGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCGGGCGCAATATTCATTCTTATTTCCGCGTTGATTCACGAACTGTTAATTACTTATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCt                                                                                                                                                                                                    <  2:800534/239‑1 (MQ=255)

                                               cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACAAAAAAAC‑AAAAAACAGAGTTTGCAT‑CTGTGGCGAAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCACGTAACTAA‑TTG‑GCAGAGAtttagcgat                                                                                                                                                                                                   >  2:147067/1‑231 (MQ=255)

                                               cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCTACAAATTC‑AAAAAATTGAGTTTCACT‑GATGAGCGCAAATAAAAAACAC‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAAATT‑ACATAGATTGTTTCTaa                                                                                                                                                                                                  >  1:800534/1‑239 (MQ=255)

                                                    tgtTTTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTa                                                                                                                                                                                             <  1:1082447/239‑1 (MQ=255)

                                                           cGCAAAACATTCCCA‑TGCCTTGCTTCGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCAAACTTTTAATTACATATA‑CTCGGTGGCAAAAAATAAAAACCAAAATTTTTGGTGGTAGTTTGGGGAGGTGAATTTTTCCAGAAAAAAAC‑AAAAACTGAGGTTGCAAT‑GATGAGCGAAAGTAAAAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTACCGCCAt                                                                                                                                                                                      <  1:618457/239‑1 (MQ=255)

                                                              aaaaatttGCCATTTCCCTG‑TCTGGCGACATTTTATTTTTTTTTTTCGGGTTGATTCACAAACTTGTTATTAAATATTACAGGGTGAAAAAAAATA‑AAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTA‑ACAGAGTTTGTCGTTAACTTTACCGCCATGAt                                                                                                                                                                                   <  2:1306196/233‑1 (MQ=255)

                                                              aaaaCATTGCCA‑TTCCCTGTTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCGAATTGTTAATTACATATT‑CACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGTTAACTTTACCGCCATGAt                                                                                                                                                                                   <  1:1220250/239‑1 (MQ=255)

                                                              aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCAT‑GATGAGCGAAAGGAATAAGAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAAAGAATGTCGCTAACTTTAACGCCATGAt                                                                                                                                                                                   >  1:251319/1‑239 (MQ=255)

                                                              aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAAAGGAGGTGCCAT‑GATGAGCGGAAGAAATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTT‑ACAGAGATTGTGGCTAAATTTATCCCCCTGAt                                                                                                                                                                                   >  1:59610/1‑239 (MQ=255)

                                                                     tGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGAAAAAAAA‑AAAAAAATGAGTTGTCAT‑TATTAGCGAAAATAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGGAAAAAATT‑AAAGAGATTGGTGCTCACTTTTACGCCATGAatttttc                                                                                                                                                                             >  1:642912/1‑234 (MQ=255)

                                                                       ccA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAATTGAGTTGTCAT‑GATGAGGGAAAGGAATAAGAAA‑‑‑‑CAA‑‑‑GCAAAAAGCA‑GCGGATAAGTTG‑ACAGAGATTTTTGCTAAATTTACCCACATGAttt                                                                                                                                                                                 >  1:558935/1‑239 (MQ=255)

                                                                         ctttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GCGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                                                                                                                                                         <  1:677346/237‑1 (MQ=255)

                                                                         a‑TTCCCTGCTCTGGCACAATATTCTTTCTTTTTTTCGCGTTGATTCACGAACGGTTAATTACATTTT‑CACGGTGGCAAAAAATATAAAACCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGaa                                                                                                                                                                        <  1:482289/239‑1 (MQ=255)

                                                                             cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAA‑AAAAACTTGAGTTTGCCT‑TTTGAGGGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ACGAATAAGATT‑AGAGAGAATGTGGGTCACTTTACCGCCCTGATTTCTATCCGAATGc                                                                                                                                                                     >  2:970454/1‑239 (MQ=255)

                                                                                      ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATCGAGTTTTCCT‑GATGTGGGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAATTT‑ACAGAGAATGTCGCTAAATTTAACTCCATGTTTTTTACCCGCATGACcgatgtgg                                                                                                                                                            >  1:623602/1‑236 (MQ=255)

                                                                                       gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAAAAAAAAACAAAAAACTGCGGTTGCC‑T‑GATGAGGGGAAGCAATTAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GCA‑CAGAATAAGTTT‑TCAGAGATTGTCGTTACCTTTATAGCCCCGCTTTCTATCCGAATGccccgggacgt                                                                                                                                                           >  2:968220/1‑230 (MQ=255)

                                                                                        cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAA‑AAAAAATGGAGTTGCCAT‑GATGAGCGAAAGAAAAAAAAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAATAAGTTG‑ACAGAGATTGGCGCTAACTTTTCCGCCATGATTTTTACCCCAATGCCtgattaaggg                                                                                                                                                          >  1:1349641/1‑233 (MQ=255)

                                                                                         gcAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATT‑CAGGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAAACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                          <  1:970454/238‑1 (MQ=255)

                                                                                             aactaattcTTATTTTCGCTTTGATACATGAACTGTAAATTCCATATA‑CACGGTGGAAAAAAATAAAAAACAAATTTTTTGGGGTTATTTTATGGGGGAGAATTGTTCAAAAAAAATAC‑AAAATCGGGAGTGGCAGT‑AAGGAAAAAAAGTAAGAAGCAG‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑ACA‑TGAAATAAGTGG‑AAGGGAATTGGCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                     <  2:1291506/234‑1 (MQ=255)

                                                                                               ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTGGGGTTTCCAT‑GATGAGCGAGAGAAATAAGCAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAATTT‑ACAGAGATTGTCGCTAAATTTACCGCCATGATTTCTACCCCCATGCCTGTTGGCGGGGGGGCTa                                                                                                                                                   >  1:1245946/1‑239 (MQ=255)

                                                                                                     tttATTTTAGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGAAAAAAAAATAAAACCACTTTTTAAGTGTGAGTTTGTGGCGTGGAATTTTTCAAGAAAAAAAC‑AAAAACTGGAGTTGCCAT‑GTGAAGAAAAATTAATAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCCCTAACTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                            <  1:968220/238‑1 (MQ=255)

                                                                                                     cTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                            <  1:328413/239‑1 (MQ=255)

                                                                                                      ttATTTTCGCGTTGATTCACGAACTGTTAATTACATTGT‑CACGGTGGAAAAAAATAGAAACCCACATTTGTAGTGGTATTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGACTGATGACGTGGTGGATAAACT‑aaa                                                                                                                                           <  1:359612/239‑1 (MQ=255)

                                                                                                       tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAC‑AAAAAATGGAGTTTGCTT‑TATGAGCGGAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑ACA‑GAGAAACAGATT‑AAAAAAATTGTAGGTAAATTTTAAGCCATGAGTTTTACCCCAATGCCTTGTTACGGGGTGGATAAACC‑aaac                                                                                                                                          >  2:958061/1‑238 (MQ=255)

                                                                                                       tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATTGAGTTTGCCT‑GATGAGAGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑ACA‑GAGAAAAAATTG‑ACAGAGATTGTTGCTAAATTTAACGCCAAGATTTTTACCCCAAAGCCCGATTACGGGGGGGATAAACT‑aaaa                                                                                                                                          >  1:459018/1‑239 (MQ=255)

                                                                                                       tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTAAGACAAAAAC‑AAAAAATAGAGTTTTCAT‑TATGAGCGGAAGAAATAAAAAA‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑AAA‑ATGAAAAAGATG‑GCAGAGAGTGTTGCTAACTATACCGCCCTGATTTTTAACCGAATGCCTGATGAAGGGGTGGATAAACA‑aaaa                                                                                                                                          >  2:475605/1‑239 (MQ=255)

                                                                                                         ttttCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTACGAAAAAAAC‑AAAAAATGGAGTTTTCAT‑TGTGGGAGGAAAGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAAAAAATTG‑ACAAAGATTGTGGCGTACTTTTTCGCCCTGATTTCTTTCCGAATACCTGATGACGTGGGGGGTATACA‑aaaaaa                                                                                                                                        >  2:582594/1‑237 (MQ=255)

                                                                                                              gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCAGACAAAAAA‑AAAAACAGTAGGGTCCTG‑TATTGGGGGAAGTTATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAAATT‑GCAGAAATTGTCGCTAACATTACCCCCCAGAATTCTTCCCAAATGCCTGATTACGCGGTGGATAAATT‑aaaaagagtta                                                                                                                                   >  2:1151044/1‑232 (MQ=255)

                                                                                                              gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAAAGGAGTTGGCAT‑TATGAGGGGAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAGATG‑GCAGAGAATGTTGCTAAATTTACCACCCTGATTTCTACCCGAATACATGATGACTGGGGGGGTAAACA‑aaaaaaaataa                                                                                                                                   >  2:1102120/1‑232 (MQ=255)

                                                                                                                 tttaTTCAGGAATTTTTAATTAAATATT‑CAGGGTGCAAAAAAATAAAAAACCCTATTTTTGGTGGTGGTTTGTGGGGGTGAATTTTTCCAAAAAAATAA‑AAAAACGGGATTTGCCAT‑GGTGAGGGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg                                                                                                                                 <  2:1245946/235‑1 (MQ=255)

                                                                                                                  gaattaccGAACTGTTAATTACATATT‑ACGGGTGGAAAAAAATATAAAACCACATTTTTAGTGTTGTTTTGTGGAGGTGAATTTTTACAGACAAATAC‑AAAATCTGGAGTGCCCGT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCG‑GTGAATAAGTTG‑ACAGAGTTTTTCACTAACTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg‑g                                                                                                                               <  2:1280570/231‑1 (MQ=255)

                                                                                                                               gTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCCT‑GATGAGGGAAAGTAATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAATAAGTTT‑ACAGAGAATTTTGCTAACTTTAACCCCATGATTTTTACCCGAAAGCCTTAATAAGTGGGGGGTAAACT‑AAAAAAGATAAAA‑GAAGGCGAAAcagcg                                                                                                                 >  1:652970/1‑235 (MQ=255)

                                                                                                                                ttAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAgaga                                                                                                                                                                                                            <  1:697647/149‑1 (MQ=255)

                                                                                                                                taaaTTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                     <  1:263711/202‑1 (MQ=255)

                                                                                                                                  aaTAACATATT‑CACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTACCAGACAAATAA‑AAAAACGGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGtc‑gtcg                                                                                                             <  2:595367/239‑1 (MQ=255)

                                                                                                                                    ttAAATATT‑CAAGGTGTAAAAAAATATAAAACCGCATTTTAAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTGGCCAT‑GATGAGCAAAAGTAATAACCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGAt                                                                                                           <  1:1068744/239‑1 (MQ=255)

                                                                                                                                        atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAC‑AAAAACTGGAGGTGGCAT‑GATGGGCGAAAGGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAAAAAATTG‑AAAGAGAGTGTCGCTAACTTTACCGCCATGATTTTTACCCGCATGGCTGATGACGTGGTGGGGAAACT‑AAAAAACCTAAAG‑GATACCGAAAAGTC‑CTCGGTgggg                                                                                                       >  1:631210/1‑239 (MQ=255)

                                                                                                                                        atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACAAATAC‑AAAAAAGGGGGGTGCCGT‑GGTGGGCTGGAGTAATAAAAAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GAA‑ATAACTAAGTTT‑TCTGACATTTGGGCTAAATTTAAATCAATGCGTTCTTCCCCAACCCCTGAGTGCGCCGGGGGTAAAAA‑AAAACAGCTAAAGAGTTTCCTGAAC‑TC‑GTCGATgggg                                                                                                       >  2:229326/1‑239 (MQ=255)

                                                                                                                                        atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGa                                                                                                                        <  1:1049051/223‑1 (MQ=255)

                                                                                                                                        atatT‑CACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTa                                                                                                                                    <  1:829964/212‑1 (MQ=255)

                                                                                                                                         tatT‑CACGTGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTGGTGGCGTTAAATTTTTACATACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGAGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTGG‑ACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                      <  1:1403772/239‑1 (MQ=255)

                                                                                                                                         tatT‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                      <  1:469404/239‑1 (MQ=255)

                                                                                                                                         tatT‑CACGGTGGAAAAAAATATAAAACCAAATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAAAAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                      <  2:1332086/239‑1 (MQ=255)

                                                                                                                                         attt‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAATTGGAGTTGCCTT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                      <  1:1064968/237‑1 (MQ=255)

                                                                                                                                          tatccgcGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGAGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTA‑ACAGATATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                      <  2:251319/233‑1 (MQ=255)

                                                                                                                                          atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGAAAAAAAA‑AAAAAATTGAGTTGGCCT‑GGTGGGTGGAAAAAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑ACA‑AGGGATAAATTG‑GAAGAGATTGTTGCTaa                                                                                                                                                                                                  >  2:697647/1‑149 (MQ=255)

                                                                                                                                          atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAAACAAAAAC‑AAAAACAGGAGGTTGCAT‑GGTGAGCGGAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑AAA‑AGGGATAAGATGTACA‑AGAATGTGGCTAAATATACCCCCATAATTTTTTACCGAAAGTATGTTGTAGGGGTGGATTAAAC‑AAAACAACTTAAA‑GAGGCCGCAACCGC‑GTCGGTggggga                                                                                                     >  2:91747/1‑237 (MQ=255)

                                                                                                                                          atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGGGGTGCCAT‑GATGGGCGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAATTTACCGCCCTGATTTCTACCCGAATGCCTGATGACGGGGGGGGTAAACT‑AAAAACGCTAAAA‑GAAGCCGAAAAGTC‑GGTGATggggga                                                                                                     >  1:1374632/1‑237 (MQ=255)

                                                                                                                                          atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAAATGAGTTTGCAT‑GGTGAGCGGAAAAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑AGGAATAAATTT‑ACAAAGAATGTCGCCAACTTTACCGCCCTTATTTTTAACCCAATGGCTGATGACGTGGGGGGTAAAAT‑AAAACAACAAAAA‑GATTCCGCAACATC‑GTCGATggggga                                                                                                     >  1:1232761/1‑237 (MQ=255)

                                                                                                                                          atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGaa                                                                                                     <  1:1151052/239‑1 (MQ=255)

                                                                                                                                          aattacgggTGGAAAAAAAAAAAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAAAAC‑AAAACCTGGAGTGGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑TTAAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                      <  1:636384/232‑1 (MQ=255)

                                                                                                                                               aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAA‑AAAAAATTGAGTTGCCCT‑GATTGGGGGAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAATTG‑ACAGAGATTTTCGCTAACTTTTCCGCCATGATTTTTACCCGAATGCCTGATTACGTGGGGGGTAAAAT‑AAAAAAGATAAAG‑GATGCCGAAAAGTC‑GGCGATGGGGGAAAtt                                                                                                 >  1:1201523/1‑239 (MQ=255)

                                                                                                                                                cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAA‑AAAAAATTGAGGTGGCCT‑GATTGGGGGAAGTAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAAATT‑ACAGAGAATGTGGCTAACTTTACCCCCAAGATTTTTATCCCGATGGACGAAAACGGGGGGGGTAAAATAAAAACACTTAAAG‑AATCCGGAAACGTG‑TCCGTGGGGGAAAAta                                                                                                 >  2:487241/1‑238 (MQ=255)

                                                                                                                                                 gagaaaaaaaaaTA‑AAAACCAAATTTTTGTGGGTGGTTTGTGAGGGTGAATTTTTCAAAAAAAATAA‑AAAAACGGGAGTTGCCTG‑GATGAGAAAAATAAAAAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAGGTTG‑ACAGAGATTGCCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                               <  2:459018/232‑1 (MQ=255)

                                                                                                                                                  gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                               <  1:958061/238‑1 (MQ=255)

                                                                                                                                                  gTGGAAAAAAAAAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAA‑AAAACCTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTTTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                               <  2:623602/238‑1 (MQ=255)

                                                                                                                                                   tGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAC‑AAAAACTGGATTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACATCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATc                                                                                              <  1:134290/238‑1 (MQ=255)

                                                                                                                                                        aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAGATTTTCCAAACAAAAAA‑AAAAAATGGAGTTGACCT‑GGTGGGCGGAAGAAAAAAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑ACA‑AAGCAAAAGATG‑GCAGAGATGTTCGATCAATTTACTGCCCTGATTTCTAACCGAAAGCCCGACTACGGGGTGGAGAAACA‑AAAACAGCCAAAG‑GAGGCCCACA‑GTCAGTCGGTGGGGAAAATTATCTACCa                                                                                         >  2:1323481/1‑238 (MQ=255)

                                                                                                                                                           aaTATAAAACCACTTTTTTAGTGGTAGTGTGTGGCGGTGAATTTTTCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGTAATAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGCCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                    <  1:748633/239‑1 (MQ=255)

                                                                                                                                                           aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTTCCAAAAAAAAAC‑AAAAACGGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                    <  2:1201523/239‑1 (MQ=255)

                                                                                                                                                           aaTAAAAACCCACATTTTGAGTGGGAGTTGGGGGGGGTGAATTTTTCCAAAAAAAAAC‑AAAAAGGGGAGTGGCAAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCG‑GTAAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                    <  1:229326/239‑1 (MQ=255)

                                                                                                                                                           aaTAAAAAACCACTTTTTTAGGGGTAGTTTGTGGGGGTGAATTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑TGGAATAAGTTG‑ACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                    <  1:40417/239‑1 (MQ=255)

                                                                                                                                                           aaTAAAAAACCACATTTGTAGTGGTAGTGTGTGGCGGTAATTTTTTCAAGAAAAATAC‑AAAAACGGGAGTTGCCAG‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAGCAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                    <  2:1374632/239‑1 (MQ=255)

                                                                                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGATG‑ACAGAGATTGTCGCCAACTTTACCGCCCTGATTTTTACCCCAATGCCTGGTGACGTGGTGGGTAAACT‑AAAACAGCTAAAA‑GATGCCCAAACGAC‑GTCGGTGGGGGAAATTATTTACCCTACAGATGTT‑Gacaaa                                                                        >  1:957057/1‑238 (MQ=255)

                                                                                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAA‑AAAAAATTGAGGTGTCAT‑GGTGAGCGAAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAAATG‑ACAAAGATTGTCGCCAACTTTTCCg                                                                                                                                                                                          >  1:77967/1‑129 (MQ=255)

                                                                                                                                                                                    ggTTTGGGGTGGAAAATTTTACAAAAAAAATAA‑AAAAACGGAAGTTGCCAT‑GATGAGAGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGGAATAAGTTG‑ACAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                           <  2:652970/238‑1 (MQ=255)

                                                                                                                                                                                    aGTTTGTGGCGGTAAATTTTTCAAAAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGGCCGAAAATAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATGGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                           <  1:1151044/239‑1 (MQ=255)

                                                                                                                                                                                          tgGCGGTGAATTTTTCCAGACAAAAAC‑AAAAATTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGCCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGAc                                                      <  1:669602/238‑1 (MQ=255)

                                                                                                                                                                                            gCGGTAAATTTTCCAAGACAAATAA‑AAAAACTGGAGTTGCCAA‑GATGAGCAAAAGTAATAAGCAAAGGGC‑AAGGG‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                           <  2:558935/238‑1 (MQ=255)

                                                                                                                                                                                                taaaTTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGA‑ACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                                                <  1:147067/236‑1 (MQ=255)

                                                                                                                                                                                                      ttaccAAACAAAAAA‑AAAAACTGAGTTTCCCAG‑GAAGAGCAAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑CGA‑GGAAATAAGTTG‑AAAGAGTGTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                         <  2:1232761/236‑1 (MQ=255)

                                                                                                                                                                                                                  tAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                             <  1:475605/239‑1 (MQ=255)

                                                                                                                                                                                                                      aaaaaCGGGATTTGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGATTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                          <  1:795382/239‑1 (MQ=255)

                                                                                                                                                                                                                        aaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                        >  2:1250640/1‑239 (MQ=255)

                                                                                                                                                                                                                         accgggAGTTGCAAT‑GATGAGGGAAAGAAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGAAAAAGGTTG‑AAAGAGATTGCCGCTACCTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:59610/235‑1 (MQ=255)

                                                                                                                                                                                                                         aaCTGGGGTTGCAAT‑AAGGAGCAAAAGTAATACGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAAAAAGTTG‑ACAGAGTTTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  2:1349641/239‑1 (MQ=255)

                                                                                                                                                                                                                         aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:615170/239‑1 (MQ=255)

                                                                                                                                                                                                                         aaCTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:905093/239‑1 (MQ=255)

                                                                                                                                                                                                                         aaCTGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:582594/239‑1 (MQ=255)

                                                                                                                                                                                                                         aaCTGAAGTTGCCAT‑GATGAGCGAAATTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAATAAGTTG‑GCAGAGATCGTACCTAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                       <  1:793625/239‑1 (MQ=255)

                                                                                                                                                                                                                          aCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                      >  2:931448/1‑239 (MQ=255)

                                                                                                                                                                                                                              gAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑CATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCACAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGCACCCGCGGAGAtt                  >  2:570219/1‑239 (MQ=255)

                                                                                                                                                                                                                                               cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                            >  2:1112121/1‑197 (MQ=255)

                                                                                                                                                                                                                                               cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg  >  1:545939/1‑239 (MQ=255)

                                                                                                                                                                                                                                               cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                            <  1:1112121/197‑1 (MQ=255)

TAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAG  >  NC_000913/3206236‑3206709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`