BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,483	G→A	22.7%	K7K (AAG→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,483	0	G	A	22.7%	181.1 / 23.4	113	K7K (AAG→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (20/66); new base A (23/3); total (43/70)
Fisher's exact test for biased strand distribution p-value = 2.59e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC  >  NC_000913/3206249‑3206716

ttCAGCATCGGTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAGT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑c                                                                                                                                                                                                                                                      <  2:927167/239‑1 (MQ=255)

 tCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑a                                                                                                                                                                                                                                                    <  1:1433478/239‑1 (MQ=255)

      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                                         <  1:588961/236‑1 (MQ=255)

      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑g‑‑‑‑‑‑g                                                                                                                                                                                                                                         <  2:597756/236‑1 (MQ=255)

       tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATAAAAATAC‑AAAAAAAGGAGTTTGCAT‑GATGAGGGAAAGAAATAAGAAA‑‑‑‑A‑A‑‑‑gtctct                                                                                                                                                                                                                                           >  2:288314/1‑234 (MQ=255)

       tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATTGAGTTGGCAT‑GATGAGCGGAAAGAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GGA‑Gt                                                                                                                                                                                                                                    >  1:398496/1‑239 (MQ=255)

        cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑Gc                                                                                                                                                                                                                                        <  1:844009/235‑1 (MQ=255)

        cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTg                                                                                                                                                                                                                                   <  1:1290429/239‑1 (MQ=255)

          ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTTGGATTGACAC‑TAGGAGGGAAAGTAATAAAAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑ATGaa                                                                                                                                                                                                                                 >  2:601997/1‑239 (MQ=255)

            aaTTATCCGCAGGTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATa                                                                                                                                                                                                                               <  2:1382686/239‑1 (MQ=255)

                aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑c                                                                                                                                                                                                                                                      <  1:1084271/223‑1 (MQ=255)

                 tCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTg                                                                                                                                                                                                                          <  1:1083770/239‑1 (MQ=255)

                                aGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATAA‑AAAAAAATGAGTTGTCATGGTTGAG‑GGAAGTAAAAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGAAAAAAGTG‑ACAGAGATGTTCGc                                                                                                                                                                                                           >  2:894581/1‑238 (MQ=255)

                                  cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACAAAAAAAC‑AAAAAACAGAGTTTGCAT‑CTGTGGCGAAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCACGTAACTAA‑TTG‑GCAGAGAtttagcgat                                                                                                                                                                                                         >  2:147067/1‑231 (MQ=255)

                                  cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCTACAAATTC‑AAAAAATTGAGTTTCACT‑GATGAGCGCAAATAAAAAACAC‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAAATT‑ACATAGATTGTTTCTaa                                                                                                                                                                                                        >  1:800534/1‑239 (MQ=255)

                                       tgtTTTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTa                                                                                                                                                                                                   <  1:1082447/239‑1 (MQ=255)

                                              cGCAAAACATTCCCA‑TGCCTTGCTTCGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCAAACTTTTAATTACATATA‑CTCGGTGGCAAAAAATAAAAACCAAAATTTTTGGTGGTAGTTTGGGGAGGTGAATTTTTCCAGAAAAAAAC‑AAAAACTGAGGTTGCAAT‑GATGAGCGAAAGTAAAAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTACCGCCAt                                                                                                                                                                                            <  1:618457/239‑1 (MQ=255)

                                                 aaaaatttGCCATTTCCCTG‑TCTGGCGACATTTTATTTTTTTTTTTCGGGTTGATTCACAAACTTGTTATTAAATATTACAGGGTGAAAAAAAATA‑AAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTA‑ACAGAGTTTGTCGTTAACTTTACCGCCATG‑At                                                                                                                                                                                        <  2:1306196/233‑1 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGTTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCGAATTGTTAATTACATATT‑CACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGTTAACTTTACCGCCATG‑At                                                                                                                                                                                        <  1:1220250/239‑1 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCAT‑GATGAGCGAAAGGAATAAGAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAAAGAATGTCGCTAACTTTAACGCCATG‑At                                                                                                                                                                                        >  1:251319/1‑239 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAAAGGAGGTGCCAT‑GATGAGCGGAAGAAATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGTTT‑ACAGAGATTGTGGCTAAATTTATCCCCCTG‑At                                                                                                                                                                                        >  1:59610/1‑239 (MQ=255)

                                                   aaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑Attt                                                                                                                                                                                      <  1:1192224/239‑1 (MQ=255)

                                                        tGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGAAAAAAAA‑AAAAAAATGAGTTGTCAT‑TATTAGCGAAAATAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GGGGAAAAAATT‑AAAGAGATTGGTGCTCACTTTTACGCCATG‑Aatttttc                                                                                                                                                                                  >  1:642912/1‑234 (MQ=255)

                                                          ccA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAATTGAGTTGTCAT‑GATGAGGGAAAGGAATAAGAAA‑‑‑‑CAA‑‑‑GCAAAAAGCA‑GCGGATAAGTTG‑ACAGAGATTTTTGCTAAATTTACCCACATG‑Attt                                                                                                                                                                                      >  1:558935/1‑239 (MQ=255)

                                                            ctttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GCGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGa                                                                                                                                                                              <  1:677346/237‑1 (MQ=255)

                                                                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAA‑AAAAACTTGAGTTTGCCT‑TTTGAGGGAAAGTAATAAAAAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ACGAATAAGATT‑AGAGAGAATGTGGGTCACTTTACCGCCCTG‑ATTTCTATCCGAATGc                                                                                                                                                                          >  2:970454/1‑239 (MQ=255)

                                                                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAAAAAAAAACAAAAAACTGCGGTTGCC‑T‑GATGAGGGGAAGCAATTAACAA‑‑‑‑C‑A‑‑‑C‑‑‑‑‑‑GCA‑CAGAATAAGTTT‑TCAGAGATTGTCGTTACCTTTATAGCCCCG‑CTTTCTATCCGAATGccccgggacgt                                                                                                                                                                >  2:968220/1‑230 (MQ=255)

                                                                           cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAA‑AAAAAATGGAGTTGCCAT‑GATGAGCGAAAGAAAAAAAAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAATAAGTTG‑ACAGAGATTGGCGCTAACTTTTCCGCCATG‑ATTTTTACCCCAATGCCtgattaaggg                                                                                                                                                               >  1:1349641/1‑233 (MQ=255)

                                                                            gcAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATT‑CAGGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAAACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                               <  1:970454/238‑1 (MQ=255)

                                                                                tatTCTATCTTATTTTCGAGTTTATTCACGAACTGTTAATTAAATATC‑CAGGGTGCCAAAAAATATAAAACCTCATTTGTAGGGGAAGTTTGTGGGGGTGAATTTTCCAAGACAAATAC‑AAAACCTGGAGTTGCCAT‑GAGTAGCAAAAATAAAAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAATTTGCCGCTATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                          <  2:1286999/239‑1 (MQ=255)

                                                                                aactaattcTTATTTTCGCTTTGATACATGAACTGTAAATTCCATATA‑CACGGTGGAAAAAAATAAAAAACAAATTTTTTGGGGTTATTTTATGGGGGAGAATTGTTCAAAAAAAATAC‑AAAATCGGGAGTGGCAGT‑AAGGAAAAAAAGTAAGAAGCAG‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑ACA‑TGAAATAAGTGG‑AAGGGAATTGGCGCAAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                          <  2:1291506/234‑1 (MQ=255)

                                                                                  ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTGGGGTTTCCAT‑GATGAGCGAGAGAAATAAGCAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAATTT‑ACAGAGATTGTCGCTAAATTTACCGCCATG‑ATTTCTACCCCCATGCCTGTTGGCGGGGGGGCTa                                                                                                                                                        >  1:1245946/1‑239 (MQ=255)

                                                                                        tttATTTTAGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGAAAAAAAAATAAAACCACTTTTTAAGTGTGAGTTTGTGGCGTGGAATTTTTCAAGAAAAAAAC‑AAAAACTGGAGTTGCCAT‑GTGAAGAAAAATTAATAAGCAC‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCCCTAACTTTTCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                                 <  1:968220/238‑1 (MQ=255)

                                                                                        cTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                                 <  1:328413/239‑1 (MQ=255)

                                                                                         ttATTTTCGCGTTGATTCACGAACTGTTAATTACATTGT‑CACGGTGGAAAAAAATAGAAACCCACATTTGTAGTGGTATTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGACTGATGACGTGGTGGATAAACT‑aaa                                                                                                                                                <  1:359612/239‑1 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAC‑AAAAAATGGAGTTTGCTT‑TATGAGCGGAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑ACA‑GAGAAACAGATT‑AAAAAAATTGTAGGTAAATTTTAAGCCATG‑AGTTTTACCCCAATGCCTTGTTACGGGGTGGATAAACC‑aaac                                                                                                                                               >  2:958061/1‑238 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAC‑AAAAAATTGAGTTTGCCT‑GATGAGAGAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑ACA‑GAGAAAAAATTG‑ACAGAGATTGTTGCTAAATTTAACGCCAAG‑ATTTTTACCCCAAAGCCCGATTACGGGGGGGATAAACT‑aaaa                                                                                                                                               >  1:459018/1‑239 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTAAGACAAAAAC‑AAAAAATAGAGTTTTCAT‑TATGAGCGGAAGAAATAAAAAA‑‑‑‑A‑A‑‑‑G‑‑‑‑‑‑AAA‑ATGAAAAAGATG‑GCAGAGAGTGTTGCTAACTATACCGCCCTG‑ATTTTTAACCGAATGCCTGATGAAGGGGTGGATAAACA‑aaaa                                                                                                                                               >  2:475605/1‑239 (MQ=255)

                                                                                                 gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAAAGGAGTTGGCAT‑TATGAGGGGAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAGATG‑GCAGAGAATGTTGCTAAATTTACCACCCTG‑ATTTCTACCCGAATACATGATGACTGGGGGGGTAAACA‑aaaaaaaataa                                                                                                                                        >  2:1102120/1‑232 (MQ=255)

                                                                                                    tttaTTCAGGAATTTTTAATTAAATATT‑CAGGGTGCAAAAAAATAAAAAACCCTATTTTTGGTGGTGGTTTGTGGGGGTGAATTTTTCCAAAAAAATAA‑AAAAACGGGATTTGCCAT‑GGTGAGGGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg                                                                                                                                      <  2:1245946/235‑1 (MQ=255)

                                                                                                     gaattaccGAACTGTTAATTACATATT‑ACGGGTGGAAAAAAATATAAAACCACATTTTTAGTGTTGTTTTGTGGAGGTGAATTTTTACAGACAAATAC‑AAAATCTGGAGTGCCCGT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCG‑GTGAATAAGTTG‑ACAGAGTTTTTCACTAACTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAgg                                                                                                                                     <  2:1280570/231‑1 (MQ=255)

                                                                                                                  gTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCCT‑GATGAGGGAAAGTAATAAGCAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GAGAATAAGTTT‑ACAGAGAATTTTGCTAACTTTAACCCCATG‑ATTTTTACCCGAAAGCCTTAATAAGTGGGGGGTAAACT‑AAAAAAGATAAAAGAAGGCGAAAcagcg                                                                                                                       >  1:652970/1‑235 (MQ=255)

                                                                                                                   ttAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAgaga                                                                                                                                                                                                                  <  1:697647/149‑1 (MQ=255)

                                                                                                                   taaaTTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                          <  1:263711/202‑1 (MQ=255)

                                                                                                                     aaTAACATATT‑CACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTACCAGACAAATAA‑AAAAACGGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                    <  2:595367/239‑1 (MQ=255)

                                                                                                                       ttAAATATT‑CAAGGTGTAAAAAAATATAAAACCGCATTTTAAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTGGCCAT‑GATGAGCAAAAGTAATAACCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                                                                                                  <  1:1068744/239‑1 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAC‑AAAAACTGGAGGTGGCAT‑GATGGGCGAAAGGAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAAAAAATTG‑AAAGAGAGTGTCGCTAACTTTACCGCCATG‑ATTTTTACCCGCATGGCTGATGACGTGGTGGGGAAACT‑AAAAAACCTAAAGGATACCGAAAAGTCCTCGGTgggg                                                                                                              >  1:631210/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATTGAGTTTTCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTAACGCCCTG‑ATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAAAT‑AAAAAAGCTAAAGGATGACGCAACGCAGGCGATgggg                                                                                                              >  1:1388078/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTATAACAAATAA‑AAAAACAGGAGTTGGCAT‑TATGAGGGGAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GAA‑GGGGAAAAGTTT‑AGAGGGAATGTTGCTACATTTATACCCATGAATTATTACCCAAAAGCC‑GGTGTTGTGGGGGATAAAAC‑AAAACAGCTAAAAAACTTCGGAAAGTCGTCGACgggg                                                                                                              >  2:1019021/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGa                                                                                                                              <  1:1049051/223‑1 (MQ=255)

                                                                                                                           atatT‑CACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTa                                                                                                                                         <  1:829964/212‑1 (MQ=255)

                                                                                                                            tatT‑CACGTGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTGGTGGCGTTAAATTTTTACATACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGAGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTGG‑ACAGAGATTGTCGTTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  1:1403772/239‑1 (MQ=255)

                                                                                                                            tatT‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  1:469404/239‑1 (MQ=255)

                                                                                                                            tatT‑CACGGTGGAAAAAAATATAAAACCAAATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAAAAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  2:1332086/239‑1 (MQ=255)

                                                                                                                            attt‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAATTGGAGTTGCCTT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  1:1064968/237‑1 (MQ=255)

                                                                                                                             tatccgcGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGAGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTA‑ACAGATATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  2:251319/233‑1 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGAAAAAAAA‑AAAAAATTGAGTTGGCCT‑GGTGGGTGGAAAAAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑ACA‑AGGGATAAATTG‑GAAGAGATTGTTGCTaa                                                                                                                                                                                                        >  2:697647/1‑149 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAAACAAAAAC‑AAAAACAGGAGGTTGCAT‑GGTGAGCGGAAGTAATAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑AAA‑AGGGATAAGATGTACA‑AGAATGTGGCTAAATATACCCCCATA‑ATTTTTTACCGAAAGTATGTTGTAGGGGTGGATTAAAC‑AAAACAACTTAAAGAGGCCGCAACCGCGTCGGTggggga                                                                                                            >  2:91747/1‑237 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAAATGAGTTTGCAT‑GGTGAGCGGAAAAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑AGGAATAAATTT‑ACAAAGAATGTCGCCAACTTTACCGCCCTT‑ATTTTTAACCCAATGGCTGATGACGTGGGGGGTAAAAT‑AAAACAACAAAAAGATTCCGCAACATCGTCGATggggga                                                                                                            >  1:1232761/1‑237 (MQ=255)

                                                                                                                             aattacgggTGGAAAAAAAAAAAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAAAAC‑AAAACCTGGAGTGGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑TTAAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                             <  1:636384/232‑1 (MQ=255)

                                                                                                                                  aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAA‑AAAAAATTGAGTTGCCCT‑GATTGGGGGAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAATTG‑ACAGAGATTTTCGCTAACTTTTCCGCCATG‑ATTTTTACCCGAATGCCTGATTACGTGGGGGGTAAAAT‑AAAAAAGATAAAGGATGCCGAAAAGTCGGCGATGGGGGAAAtt                                                                                                        >  1:1201523/1‑239 (MQ=255)

                                                                                                                                   cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAA‑AAAAAATTGAGGTGGCCT‑GATTGGGGGAAGTAAAAAACAA‑‑‑‑A‑A‑‑‑A‑‑‑‑‑‑GCA‑ATGAATAAAATT‑ACAGAGAATGTGGCTAACTTTACCCCCAAG‑ATTTTTATCCCGATGGACGAAAACGGGGGGGGTAAAATAAAAACACTTAAAGAATCCGGAAACGTGTCCGTGGGGGAAAAta                                                                                                        >  2:487241/1‑238 (MQ=255)

                                                                                                                                    gagaaaaaaaaaTA‑AAAACCAAATTTTTGTGGGTGGTTTGTGAGGGTGAATTTTTCAAAAAAAATAA‑AAAAACGGGAGTTGCCTG‑GATGAGAAAAATAAAAAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAGGTTG‑ACAGAGATTGCCGATAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                      <  2:459018/232‑1 (MQ=255)

                                                                                                                                     ggggCAAAAAAAAAAAAACCAAATGTTTAGTGGTAGTTTGGGGCGGTGAATATTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAATAAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAGATTGTCGCAACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAGTAt                                                                                                      <  2:576654/236‑1 (MQ=255)

                                                                                                                                     gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                      <  1:958061/238‑1 (MQ=255)

                                                                                                                                     gTGGAAAAAAAAAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAA‑AAAACCTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTTTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                      <  2:623602/238‑1 (MQ=255)

                                                                                                                                      tGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAC‑AAAAACTGGATTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACATCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATc                                                                                                     <  1:134290/238‑1 (MQ=255)

                                                                                                                                              aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTTCCAAAAAAAAAC‑AAAAACGGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  2:1201523/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAACCCACATTTTGAGTGGGAGTTGGGGGGGGTGAATTTTTCCAAAAAAAAAC‑AAAAAGGGGAGTGGCAAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCG‑GTAAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  1:229326/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAAACCACTTTTTTAGGGGTAGTTTGTGGGGGTGAATTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGAAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑TGGAATAAGTTG‑ACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  1:40417/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAAACCACATTTGTAGTGGTAGTGTGTGGCGGTAATTTTTTCAAGAAAAATAC‑AAAAACGGGAGTTGCCAG‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAGCAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑g                                                                                           <  2:1374632/239‑1 (MQ=255)

                                                                                                                                                tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCt                                                                                                                                                                                                          <  2:77967/129‑1 (MQ=255)

                                                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAA‑‑‑‑C‑A‑‑‑A‑‑‑‑‑‑GCA‑GTGAATAAGATG‑ACAGAGATTGTCGCCAACTTTACCGCCCTG‑ATTTTTACCCCAATGCCTGGTGACGTGGTGGGTAAACT‑AAAACAGCTAAAAGATGCCCAAACGACGTCGGTGGGGGAAATTATTTACCCTACAGATGTT‑Gacaaa                                                                               >  1:957057/1‑238 (MQ=255)

                                                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAA‑AAAAAATTGAGGTGTCAT‑GGTGAGCGAAAAAAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GGA‑GTGAATAAAATG‑ACAAAGATTGTCGCCAACTTTTCCg                                                                                                                                                                                                >  1:77967/1‑129 (MQ=255)

                                                                                                                                                                       ggTTTGGGGTGGAAAATTTTACAAAAAAAATAA‑AAAAACGGAAGTTGCCAT‑GATGAGAGAAAGTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGGAATAAGTTG‑ACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                  <  2:652970/238‑1 (MQ=255)

                                                                                                                                                                       aGTTTGTGGCGGTAAATTTTTCAAAAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGGCCGAAAATAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATGGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                  <  1:1151044/239‑1 (MQ=255)

                                                                                                                                                                             tgGCGGTGAATTTTTCCAGACAAAAAC‑AAAAATTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGAc                                                             <  1:669602/238‑1 (MQ=255)

                                                                                                                                                                               gCGGTAAATTTTCCAAGACAAATAA‑AAAAACTGGAGTTGCCAA‑GATGAGCAAAAGTAATAAGCAAAGGGC‑AAGGG‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATGGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                                  <  2:558935/238‑1 (MQ=255)

                                                                                                                                                                                   taaaTTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGA‑ACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                                                       <  1:147067/236‑1 (MQ=255)

                                                                                                                                                                                         ttaccAAACAAAAAA‑AAAAACTGAGTTTCCCAG‑GAAGAGCAAAAGTAATAAACAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑CGA‑GGAAATAAGTTG‑AAAGAGTGTGTCGTAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                <  2:1232761/236‑1 (MQ=255)

                                                                                                                                                                                                     tAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                    <  1:475605/239‑1 (MQ=255)

                                                                                                                                                                                                      taaaaaaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                    <  1:642776/236‑1 (MQ=255)

                                                                                                                                                                                                         aaaaaCGGGATTTGCCAG‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGATTTTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                 <  1:795382/239‑1 (MQ=255)

                                                                                                                                                                                                           aaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                               >  2:1250640/1‑239 (MQ=255)

                                                                                                                                                                                                            accgggAGTTGCAAT‑GATGAGGGAAAGAAAAAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GGAAAAAGGTTG‑AAAGAGATTGCCGCTACCTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  2:59610/235‑1 (MQ=255)

                                                                                                                                                                                                            aatgtgAGTTGCCGT‑GAGGAGAGAAAGAAAAAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GGTAATAAGTGG‑ACAGAGAGTGGCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  2:212011/234‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGGGTTGCAAT‑AAGGAGCAAAAGTAATACGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAAAAAGTTG‑ACAGAGTTTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  2:1349641/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑G‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:615170/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:794496/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:582594/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGAAGTTGCCAT‑GATGAGCGAAATTAATAAGAAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GAA‑GTAAATAAGTTG‑GCAGAGATCGTACCTAATTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                              <  1:793625/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                             >  2:931448/1‑239 (MQ=255)

                                                                                                                                                                                                                 gAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGCATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCACAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGCACCCGCGGAGAtt                         >  2:570219/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                                   >  2:1112121/1‑197 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg         >  1:561009/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg         >  1:545939/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                                   <  1:1112121/197‑1 (MQ=255)

                                                                                                                                                                                                                                     aaGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCg      >  1:909508/1‑239 (MQ=255)

                                                                                                                                                                                                                                         aaaaaGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GGGAAAAATTAG‑AAAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc  <  1:355740/236‑1 (MQ=255)

                                                                                                                                                                                                                                          ataaGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc  >  2:137945/1‑238 (MQ=255)

TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAA‑‑‑‑C‑A‑‑‑G‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTC  >  NC_000913/3206249‑3206716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`