Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	45 [0]	[0] 45	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	44 (0.900)	38/282	0.3	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGT  >  NC_000913/4001510‑4001792                                                                                                                                             |                                                                                                                                               gatggattgatcttgtcgaatcggaggacgacgagcgactgcgcgtacaatttgagtttggccagagcctggcaacccgcccggaacgggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatctc                                                                                                                                              <  2:674111‑M2/3‑1 (MQ=255)  attgattgatctcgtcgaacgggacgacgcggagcgactgtgcggacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                             <  2:524534‑M2/4‑1 (MQ=255)  atggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                             <  2:590992‑M2/4‑1 (MQ=255)  atggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTcc                                                                                                                                             <  1:258844‑M2/4‑1 (MQ=255)        tgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgacgccgacgacggcctgcatCTCcttcttc                                                                                                                                       >  1:738868‑M2/134‑143 (MQ=255)            cttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgccgccggcctgcatCTccttcttcttcg                                                                                                                                   >  1:876553‑M2/130‑142 (MQ=255)               gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                                >  1:586303‑M2/127‑143 (MQ=255)               gtcgaaccgcacgccgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                                <  1:765966‑M2/17‑1 (MQ=255)                      cggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgtccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                                         <  2:238931‑M2/24‑1 (MQ=255)                            acgacgagcgactgcgcgtacaatctgaacttggccagagcctgacaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                                         <  2:569840‑M2/24‑1 (MQ=255)                            acgacgagcgactgcgcgtacaatctgaacttggccagagcctgacaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGc                                                                                                                         >  1:569840‑M2/114‑137 (MQ=255)                              gacgagcgacggcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATc                                                                                                                 <  1:348622‑M2/32‑1 (MQ=255)                               acgagcgactgcgcgtacaatctggacttggccagagcctggcaactcgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCa                                                                                                                <  2:212434‑M2/33‑1 (MQ=255)                                       ctgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTa                                                                                                        <  2:311497‑M2/41‑1 (MQ=255)                                           gcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTc                                                                                                    <  2:349574‑M2/45‑1 (MQ=255)                                                 aatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctatgaagacgacgacggcctgcatCTCCTGCTTCTTCTTTGAAGATGCGCAAGATCACGCCGGTAACTCCACtgt                                                                                              >  2:753765‑M2/93‑143 (MQ=255)                                                    cggaactggacgagggcctggcgacccgcccggaactggaagacatcgaagcagcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGc                                                                                           <  2:807437‑M2/54‑1 (MQ=255)                                                     tgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCCGAAGATCACGCCGGTAACTCCACTGTGGCa                                                                                          >  1:819508‑M2/89‑143 (MQ=255)                                                     tgaactgggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCa                                                                                          <  2:505712‑M2/55‑1 (MQ=255)                                                          ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAc                                                                                     >  1:522847‑M2/84‑143 (MQ=255)                                                                agagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttggagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGCTGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTCCCatcgg                                                                               >  1:44506‑M2/78‑141 (MQ=255)                                                                     ctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                                          <  1:381215‑M2/71‑1 (MQ=255)                                                                     ccggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                                          <  1:83286‑M2/71‑1 (MQ=255)                                                                       ggcaacccgccgggacctgcaagacatcgaagcagcggcacgtttctttgaagacgacgacggcctgcatCTC‑‑‑CTTCTTCTTTGAAGATGCGGAACATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtcgt                                                                     <  1:848240‑M2/73‑1 (MQ=255)                                                                        gcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGCGacggcc                                                                       >  1:847053‑M2/70‑141 (MQ=255)                                                                                 ccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCGGTTTa                                                              >  1:359191‑M2/61‑143 (MQ=255)                                                                                  cggaactggaagacatcgaagcatcggcacgtttctttgcagacgacgccggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGCCAGTTCCAACCCGTGCTGGTCGCAGGTTTAc                                                             >  2:204422‑M2/60‑143 (MQ=255)                                                                                    gaactggaagacatcggagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTActc                                                           <  1:508025‑M2/86‑1 (MQ=255)                                                                                     aactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTActct                                                          >  1:294693‑M2/57‑143 (MQ=255)                                                                                       ctggaaaacatcgaagcatcagcacgctgcttggaagacgacgacggcctgcagCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgc                                                        <  1:63939‑M2/89‑1 (MQ=255)                                                                                        tggaagacatccaagcatcggcacgcttctttgaagacgacgacggcctgcatCTCCTGCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGCGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcg                                                       <  1:271794‑M2/90‑1 (MQ=255)                                                                                         ggaagacatcgaagcgtcggcacgtttctgtgaagacgacgacggcctgcatCTCCTGCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgt                                                      <  1:356857‑M2/91‑1 (MQ=255)                                                                                            agacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGGCGTCTGTTTACtctgtgtggg                                                   >  2:494843‑M2/50‑141 (MQ=255)                                                                                               catcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACCGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagcgt                                                <  1:826578‑M2/97‑1 (MQ=255)                                                                                                                 tttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCGcgcttttggg                              >  1:135803‑M2/29‑140 (MQ=255)                                                                                                                   tctttgaagccgacggcggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCt                            <  1:433190‑M2/117‑1 (MQ=255)                                                                                                                   tctgtgaagacgacgacggcctgcatCTCCTTCTCCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCTCTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCt                            <  1:190590‑M2/117‑1 (MQ=255)                                                                                                                          agacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAACATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGt                     >  1:848618‑M2/20‑143 (MQ=255)                                                                                                                           gacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa                    >  1:424303‑M2/19‑143 (MQ=255)                                                                                                                            acggcgacggcctgcatCGCCTTCGACGGCTCGGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTAt                   <  1:65570‑M2/126‑1 (MQ=255)                                                                                                                                    gggcggcagCTCCGTCTTCTTCTTGGAAGATGCGGACGATCATGCCGGTAACTCTACGGTGGCATTAACCAGCCGTGATGGTCGTCTGTTGACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGccc           <  2:770012‑M2/134‑1 (MQ=255)                                                                                                                                     gcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCg          >  1:822132‑M2/9‑143 (MQ=255)                                                                                                                                         gcatCTCCTTCTTGTTCTTTGAAGATGCGGAAGATTTCGCCGTTAATTCCACTGTGGCATTTACCATCCGTGTTGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGc      <  2:418510‑M2/139‑1 (MQ=255)                                                                                                                                         gcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGACCTGCCCGCTTTTCTTCTGTATCGTCTgcgtgccctcagc      >  2:105098‑M2/5‑138 (MQ=255)                                                                                                                                             cTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGt  >  1:128272/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                               ATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCGTAGCCAGT  >  NC_000913/4001510‑4001792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑