Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	701,362	G→T	S78Y (TCC→TAC)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	701,362	0	G	T	100.0%	121.7 / NA	43	S78Y (TCC→TAC)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/18);  total (25/18)

TGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG  >  NC_000913/701227‑701501                                                                                                                                         |                                                                                                                                            tGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCCCTTTGCTGCTGAGATCAAACAGAGTGCTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGCAAT‑GGCGGGCTTCGCTGACGCTGTAGATAgc                                                                                                                                      >  2:594393/1‑143 (MQ=255)     gggTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGCTGTAGATAGCgagg                                                                                                                                  >  2:212398/1‑143 (MQ=255)            ttCGGGCAGCGGTTAATGTTTTTCGGCGAGTACTTTGCTGCTGAGATCAACTAGAGTGATGGTGGCGTCATTACGACCAAGCCGTACGCCGATGGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGcccc                                                                                                                           <  2:566889/143‑1 (MQ=255)                    gCGGGTAATGTTCGTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAg                                                                                                                   <  1:809277/143‑1 (MQ=255)                     cGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAgg                                                                                                                  <  1:847831/143‑1 (MQ=255)                      gggTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGc                                                                                                                 <  1:193613/143‑1 (MQ=255)                        gTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGCTCAAACAGCGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGGGGAAAT‑TGCGGGTTGCGGTGACGACGTAGATAGCGCAGCGGCCCCCGGCGGAGGccc                                                                                                               >  2:736598/1‑142 (MQ=255)                        gTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCct                                                                                                               >  1:136029/1‑143 (MQ=255)                         tAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATAAAACAGAGTGATGGTGGCGTCATGACGACGAGGCCGTACGCCGCCTG‑TAGAAAGTATCCCAGCTTTGGTGGCGATGTAGCTGTCGCGGCGGCCCCCGGTGGAGGCctg                                                                                                              >  1:647058/1‑143 (MQ=255)                           aTGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCctgct                                                                                                            >  1:828580/1‑143 (MQ=255)                            tGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGTCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGGTGTAGATGGCGCGGCGGCCCCCGGTGGAGGCctgctg                                                                                                           >  1:542688/1‑143 (MQ=255)                                  tctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCTATGGCTTGGACAA‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAAc                                                                                                     <  1:242780/143‑1 (MQ=255)                                     gcgaGCACTTTGCTGCGGAGGTCAAACAGTGTGGTGGTGGCGGCAGGGCGACCAAGCCGTACGCCGATTGCGTGGAAAT‑GGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAActtc                                                                                                  <  2:823323/140‑1 (MQ=255)                                      ccAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGGCCCCGGTGGAGGCCTGCTGATCAActtct                                                                                                 <  1:846389/143‑1 (MQ=255)                                       cAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAActtctt                                                                                                >  1:303053/1‑143 (MQ=255)                                        aGCTCTTTGCTGCTGGGATCGAGCAGGTTCATGTTGGCGTCTTGACGACCTAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCttt                                                                                               <  2:564419/143‑1 (MQ=255)                                         gCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTg                                                                                              >  1:614292/1‑143 (MQ=255)                                           aCTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGAt                                                                                            <  1:832790/143‑1 (MQ=255)                                            cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGGGACATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATc                                                                                           <  1:63130/143‑1 (MQ=255)                                                  tgctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCcg                                                                                     >  1:4778/1‑143 (MQ=255)                                                      gagaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGtt                                                                                 >  1:67099/1‑143 (MQ=255)                                                        gaTCGAACAGGTTGATGGTCGCGTTCTGGCGACCGGGCCGTACGCCGATGGCCTGGAAAT‑TGAGGGTTTCTGTTACGATGTAGATAGCGCGGCGGTTCCCGGTCGAGGCCTGTTGATAAACTTCTTTGATCAGCCCGCGTTCg                                                                               <  2:645986/143‑1 (MQ=255)                                                         gtCAAACAGAGTGGTGGGGGCGTCATGACGTCCCCGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGa                                                                              <  2:119917/142‑1 (MQ=255)                                                         aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAGAT‑TGCGGGGTGCGGCGAGGATGTAGATAGCGCGGCGGGCCCCGGTCGAGGCCTGCGGCGCCAGTTCTTGGAGCAGCGCCCGCTCGa                                                                              >  2:612159/1‑143 (MQ=255)                                                         aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGa                                                                              >  1:871799/1‑143 (MQ=255)                                                         aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGGTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTGCGa                                                                              >  2:457986/1‑143 (MQ=255)                                                              aCAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGc                                                                         >  2:362632/1‑143 (MQ=255)                                                                        ggtggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTaa                                                               <  1:832665/143‑1 (MQ=255)                                                                          tggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAtt                                                             >  1:630660/1‑143 (MQ=255)                                                                              gTCATGACCACCAAGCCGTAAGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg                                                         <  1:212398/143‑1 (MQ=255)                                                                                   gacgacCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTCATTTTGCTTCCg                                                    >  1:312464/1‑143 (MQ=255)                                                                                         cAAGCCGTACGCCGATTGCATGGAAAT‑TGCGGGCTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCCGAGGAGGCCTGCTGATCAACTCCCCTGATCAGCCCCCGGGCGGCAAGCAGACGCATACTTCTGGCTACggccgcc                                              >  2:374379/1‑137 (MQ=255)                                                                                           tgCCGTAGGCCGATTGGGTGGGCCT‑TGCGGGTTTCGGTGGCGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCggg                                            <  2:293146/142‑1 (MQ=255)                                                                                                   gCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGCTCAGCCCGCGGTCGGTAAGCTGACGCGTAATTTGGGGTACGCTGGCGGGGGCCAggc                                    >  2:768836/1‑141 (MQ=255)                                                                                                   gCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGGAAGCt                                    >  1:546852/1‑143 (MQ=255)                                                                                                       aTTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCt                                <  2:303053/143‑1 (MQ=255)                                                                                                              ggAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGCCTTTGCTCg                         >  2:737016/1‑143 (MQ=255)                                                                                                               gAAAT‑TGCGGGTTTCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCCGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTCACGCTGGCGGGGGCAAGCTGGCTTTGCTCgg                        >  2:554827/1‑143 (MQ=255)                                                                                                                  ct‑tGCGGGTTTCGGTGGCGATGTAGATGGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTTGCTTTGCTCGGCaa                     <  1:85048/142‑1 (MQ=255)                                                                                                                             tCGGTGACGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTAAATCcg           <  1:888023/143‑1 (MQ=255)                                                                                                                                    cGATGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGAtt    >  2:798748/1‑143 (MQ=255)                                                                                                                                      aTGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATGCGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCACTCTGAATCCGCCAGATTgg  >  2:356873/1‑143 (MQ=255)                                                                                                                                      aTGTAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTgg  >  2:70133/1‑143 (MQ=255)                                                                                                                                         |                                                                                                                                            TGCTGGGTACGTTCCGGCAGCGGGTAATGTTCTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAAT‑TGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGG  >  NC_000913/701227‑701501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑