New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3365206 =59 (1.050)4 (0.070) 4/272 8.0 6.8% pseudogene (22/367 nt) yhcE pseudogene fragment; interrupted by IS5
?NC_000913 3365242 = 54 (1.000)pseudogene (58/367 nt) yhcE pseudogene fragment; interrupted by IS5
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGACATGGTGCTAGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAA  >  NC_000913/3365074‑3365347
                                                                                                                                    |                                                                                                                                              
ggACATGGTGCTAGATATAAAAGATGAGCAGCAATCATGCCGAACTCACCTGGTAATCCCATAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCctgctgct                                                                                                                                      <  1:229381/143‑1 (MQ=255)
ggACATGGTGCCAGATATAAAAGATGGGCAGCAATCATGCCGTATTCGCCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCctgctgct                                                                                                                                      <  1:959453/143‑1 (MQ=255)
cgACATGGTGCTAGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCctgctgct                                                                                                                                      <  1:253359/142‑1 (MQ=255)
   cATGGTGCTAGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGaa                                                                                                                                   <  2:388297/143‑1 (MQ=255)
   cATGGTGCTAGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGaa                                                                                                                                   <  2:836555/143‑1 (MQ=255)
        tGCTAGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCGGAACTTCg                                                                                                                              >  1:578744/1‑143 (MQ=255)
         gCTAGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGc                                                                                                                             <  1:785581/143‑1 (MQ=255)
            aGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAAGAGGATGCCTGCTGCTGAACTGCGCCag                                                                                                                          >  1:16718/1‑142 (MQ=255)
               tataAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATCATAACAGGATGCCTGCTGCTGCACTTGGCCATGGc                                                                                                                       >  1:763625/1‑143 (MQ=255)
                   aaaGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGCTGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGGGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCt                                                                                                                   >  1:634319/1‑143 (MQ=255)
                    aaGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCGGAACTTAGCCATGGCCGCtg                                                                                                                  >  2:287663/1‑142 (MQ=255)
                    aaGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGAGGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTc                                                                                                                  >  1:854427/1‑143 (MQ=255)
                     aGATGAGCGGCAATTAGGCCGTATTCACCATAAACAACCAGAAGATGCGCCAAATGATGTGTTCTATGGGACAGCAACAGGAGAGTGCCAATGATACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCtga                                                                                                                 <  2:8315/143‑3 (MQ=255)
                      gATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAg                                                                                                                <  2:477552/143‑1 (MQ=255)
                       acgAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAgg                                                                                                               <  1:402102/141‑1 (MQ=255)
                           gcagcaCGCAGGCCGTATTCACCTTACATGCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga                                                                                                           <  2:524264/143‑1 (MQ=255)
                           gcagcaATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCaga                                                                                                           <  2:409385/143‑1 (MQ=255)
                                 aTCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGACCTTCGCCATGGCCGCTCAGGCAGAGGGTaa                                                                                                     >  1:554888/1‑143 (MQ=255)
                                       ccGTATTCACCTTACGTTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATAtctc                                                                                               <  2:940406/143‑1 (MQ=255)
                                        cGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTcc                                                                                             <  1:447728/143‑1 (MQ=255)
                                         gTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCa                                                                                            <  1:878074/143‑1 (MQ=255)
                                                ccTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCAt                                                                                     >  2:200944/1‑143 (MQ=255)
                                                  ttACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCCGAGTGTAATATCTCCAGTAGCATAc                                                                                   >  1:732178/1‑143 (MQ=255)
                                                            agaagaTGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGGAGCAGACAAAATATCGa                                                                         >  2:710684/1‑143 (MQ=255)
                                                             gaagaTGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGAc                                                                        <  2:347622/143‑1 (MQ=255)
                                                               agaTGCACCAAAAGATGTGTTCTAGGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTa                                                                      <  1:477580/143‑1 (MQ=255)
                                                                      cgagaaGGTGTGTGCTATTGGAGAGCAACAGGGGAGTGCCAATGAAACGGATACTAAGAGGATGCCGGCGGCGGAACTTCGCCATGGCCGCTGAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACGATGGCaaa                                                               <  2:805375/139‑1 (MQ=255)
                                                                      ccAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATAGCaaa                                                               >  1:680971/1‑143 (MQ=255)
                                                                       cAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCAGGGCCGATCAGGCAGAGTGTAATATCTCCAATAGCATACAAAATATCGGCTATGGCAAAc                                                              >  2:339307/1‑143 (MQ=255)
                                                                        aaaaGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACg                                                             <  1:249832/143‑1 (MQ=255)
                                                                          aaGAGGCGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGaa                                                           <  1:726253/143‑1 (MQ=255)
                                                                                tgtTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGtgctg                                                     <  2:543009/143‑1 (MQ=255)
                                                                                  ttCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGcc                                                   >  1:611202/1‑143 (MQ=255)
                                                                                     tATGAGACAGCAACAGGAGAGTGCAAATAAAACAGATAATAACAGGATGCCTGATGATAAACTTCGCACTGGAAGACCAGGCATAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCAtc                                                >  2:214112/1‑142 (MQ=255)
                                                                                        gagaCAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTTCGGACGAGTGTAATGTCACCAGTAGCATACAAAATATCGACTATGGCAAAAGAAGTGCGGCCGTgggc                                             >  1:48729/1‑143 (MQ=255)
                                                                                           accggaaCAAGAGAGTGCCAATGAGACGGATAATAACAGGACGCCTGCTGCTTAACTTCGCCATGGCCGCTCACGCAGAGTGTAATATCTCCAGTAGCATACTGCATTTCGCCGATTCCAGACAAAGTTTGGCCATGCGCCaa                                          <  2:995350/138‑1 (MQ=255)
                                                                                             aGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGCGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGt                                        >  2:104585/1‑143 (MQ=255)
                                                                                               cAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTg                                      >  2:280743/1‑143 (MQ=255)
                                                                                                aaCAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGa                                     >  2:627518/1‑143 (MQ=255)
                                                                                                aaCAGCAGACTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGa                                     <  1:819306/143‑1 (MQ=255)
                                                                                                 aCATGAGTGTGCCAGTGATACGTTTAATGACAGTATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGAc                                    <  2:70258/143‑1 (MQ=255)
                                                                                                 aCAGGAGAATGCCAATGAAACAGATAATAACATGATGACTGCTGCTGAACTTCGCCATGGAGGCTCAGGCAGAGTGTAATATCTCCAGTAGTATACAAAATATCGACTATGGCAATCGAAGTGCTGCCATGCGCCAAGTTGAc                                    <  2:1600/143‑1 (MQ=255)
                                                                                                    ggAGAGTGCCAAGGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCgg                                 <  2:631584/143‑1 (MQ=255)
                                                                                                     gtgcgTGCGAATGCATCGGACAATACCAGGATGCCTGCTGCGGGACTGCGCCGTGGCCGCTGAGGCAGAGTGTAATATCGCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCAGTGCGCCAAGTTGACCggg                                <  2:412696/139‑1 (MQ=255)
                                                                                                     gagaGTGCCAATGAAACGGATAATAACAGGCTGCCTGCTGCTGACCTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTGTGGCAAACGAAGTGCTGCCCAGCGCCACGTTGAACggg                                >  2:28959/1‑143 (MQ=255)
                                                                                                       gagTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGGTGCCATGCGCCAAGTTGACCGGGGt                              >  1:861806/1‑143 (MQ=255)
                                                                                                            ccAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAaac                         >  2:303394/1‑143 (MQ=255)
                                                                                                               aTGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGGAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCCTGCGCCAAGTTGACCGGGGTAAaacaac                      >  2:766812/1‑143 (MQ=255)
                                                                                                                tgaggcGGCTAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAacaaca                     <  2:705343/138‑1 (MQ=255)
                                                                                                                   aaCGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACaa                  >  2:906135/1‑143 (MQ=255)
                                                                                                                   aaCGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGACATGCGCCAAGTGGACAGGGGTAAAACAACACaa                  >  1:411757/1‑143 (MQ=255)
                                                                                                                    aCGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGGTAAGGTAGAGTGTAAAAGCCCACACCGGAAGATAATATGCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAAt                 >  1:470161/1‑143 (MQ=255)
                                                                                                                    aCGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAAt                 >  1:588903/1‑143 (MQ=255)
                                                                                                                     cGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAACCAACACAGtt                >  2:82510/1‑143 (MQ=255)
                                                                                                                      ggctaataaCAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCCTACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTa               <  1:287663/140‑1 (MQ=255)
                                                                                                                      ggATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGAGTAAAACAACACAAtaa               >  1:613475/1‑141 (MQ=255)
                                                                                                                           ataaCAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGcaga          <  1:902036/143‑1 (MQ=255)
                                                                                                                               cAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATATCGAGTAGCACACAACCTATCGCCAACGGCAAACGAAGTGCTGCCATGCGCCAAGTCGACCGGGGGAAAACAACACCATTAGcagacaga      >  1:469738/1‑143 (MQ=255)
                                                                                                                                   aTGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGGCCGGGGTAAAACTACACAATGAGCAGACAGAACaa  >  1:371602/1‑143 (MQ=255)
                                                                                                                                    |                                                                                                                                              
GGACATGGTGCTAGATATAAAAGATGAGCAGCAATCATGCCGTATTCACCTTACATTCCCAGAAGATGCACCAAAAGATGTGTTCTATGAGACAGCAACAGGAGAGTGCCAATGAAACGGATAATAACAGGATGCCTGCTGCTGAACTTCGCCATGGCCGCTCAGGCAGAGTGTAATATCTCCAGTAGCATACAAAATATCGACTATGGCAAACGAAGTGCTGCCATGCGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAA  >  NC_000913/3365074‑3365347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.