Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,049,903 IS2 (–) +5 bp noncoding (5‑9/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4049903 =0 (0.000)65 (1.150) 56/284 0.0 100% noncoding (5/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 40499070 (0.000)71 (1.260) 55/284 0.0 100% noncoding (9/109 nt) spf Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region

Only 100 of 136 total aligned reads displayed.

CATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTT  >  NC_000913/4049765‑4050049
                                                                                                                                              |                                                                                                                                              
tgtggggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTAc                                                                                                                                                <  1:586689‑M1/5‑1 (MQ=255)
 gtgtggtacgccgtctgcgtttctccggacctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtatggagattcagagggcaagcctaGGTACa                                                                                                                                               <  2:328483‑M1/6‑1 (MQ=255)
   gtggtacgccgtctgcgttgctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACaga                                                                                                                                             >  1:395418‑M1/136‑143 (MQ=255)
   gCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtgg                                                                                                                                             >  2:121998‑M1/1‑140 (MQ=255)
   gCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtgg                                                                                                                                             <  2:418840‑M1/143‑4 (MQ=255)
   gCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtgg                                                                                                                                             >  2:668966‑M1/1‑140 (MQ=255)
    tggtacgccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcactgatttgagtgatattggttgtcgggaggttaagggggccagtctgGGTACagag                                                                                                                                            <  1:538411‑M1/9‑1 (MQ=255)
         tttCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgc                                                                                                                                       <  2:894711‑M1/143‑10 (MQ=255)
          gccgtctgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGa                                                                                                                                      <  1:816816‑M1/15‑1 (MQ=255)
           tCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgccc                                                                                                                                     <  1:217620‑M1/143‑12 (MQ=255)
           ccgTAAGTAAGCTACCTAAGGTGTCGCGTTTTGTGATGCCTATTACAAATTCTTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgccc                                                                                                                                     <  2:552272‑M1/142‑12 (MQ=255)
             gtaagtaaGCAACATAAGCTGTCACGGTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGGACTGAACACACAAGCGGCAAGTTTGTCGCGGTGGGTTTtggatttgcccct                                                                                                                                   >  1:351702‑M1/1‑130 (MQ=255)
                tgcgtttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCt                                                                                                                                <  1:108974‑M1/21‑1 (MQ=255)
                    tttctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaagtagtattaagactatcacttatttaagtgatattggttgtctggggattcagggggccagtctaGGTACAGAGGTGAGAGGTTCTATCt                                                                                                                            <  1:438594‑M1/25‑1 (MQ=255)
                    agCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTAACCAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggattagcgcctatacttc                                                                                                                            >  2:878032‑M1/1‑123 (MQ=255)
                     gCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcc                                                                                                                           >  1:916909‑M1/1‑122 (MQ=255)
                      tctccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCttt                                                                                                                          >  1:994273‑M1/117‑143 (MQ=255)
                      cAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcca                                                                                                                          <  1:268841‑M1/143‑23 (MQ=255)
                      cAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttcca                                                                                                                          <  1:100082‑M1/143‑23 (MQ=255)
                        tccggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtataaagactatcacttatttaagtgatcttggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCa                                                                                                                        <  1:42185‑M1/29‑1 (MQ=255)
                         cATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggaattgcccctatattcccagac                                                                                                                       >  2:489034‑M1/1‑118 (MQ=255)
                          cggccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggcgattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGa                                                                                                                      <  2:346824‑M1/31‑1 (MQ=255)
                             ccctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCt                                                                                                                   >  2:944523‑M1/110‑143 (MQ=255)
                              cctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCtt                                                                                                                  >  1:500284‑M1/109‑143 (MQ=255)
                               ctaagacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagagggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCttt                                                                                                                 <  2:965847‑M1/36‑1 (MQ=255)
                                 gTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgtta                                                                                                               <  1:880700‑M1/143‑34 (MQ=255)
                                  agacatcaatcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTAc                                                                                                              <  1:207171‑M1/39‑1 (MQ=255)
                                    acatcaatcagcagttcgcctatgactagtctaaacgctaggattaagactatcacttatccgagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTGCAGACCTTTTACtt                                                                                                            >  2:269349‑M1/103‑143 (MQ=255)
                                    aCGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatca                                                                                                            >  1:301667‑M1/1‑107 (MQ=255)
                                      gTTTTGTGATGGCTAATAGAAATTCCTAAGCAAGAACTTAAAAAAAAGTACAATAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcact                                                                                                          <  2:209399‑M1/143‑39 (MQ=255)
                                        caatcatctgttctccaatgactaggctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACg                                                                                                        >  1:875683‑M1/99‑143 (MQ=255)
                                         ttctgatgGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaa                                                                                                       <  1:360209‑M1/140‑42 (MQ=255)
                                           tgtatggCTATTAGAAATTCATAGGAAACAGTTGGGCACAACTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacc                                                                                                     <  2:714422‑M1/140‑44 (MQ=255)
                                           tcatctgttctccaatgactagtctaaaaactagtattaagcctatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGCGGTAAGATGTTCTATCTTTCAGACCTTTTCCTTCACGtag                                                                                                     <  1:964797‑M1/48‑2 (MQ=255)
                                           tcatctgttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggggattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTaa                                                                                                     <  1:765133‑M1/48‑1 (MQ=255)
                                           gtgATGGCTAGTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacc                                                                                                     <  1:250358‑M1/143‑44 (MQ=255)
                                             atctgttctccaatgtctagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATc                                                                                                   >  2:470111‑M1/94‑143 (MQ=255)
                                              aTGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGGACGAAAAATAGTAAAGTTAGTCGCTTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccat                                                                                                  <  1:469876‑M1/143‑47 (MQ=255)
                                               tGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGCACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttagcccatt                                                                                                 >  2:557569‑M1/1‑96 (MQ=255)
                                                ggCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccatta                                                                                                <  1:388923‑M1/143‑49 (MQ=255)
                                                 gttctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGAt                                                                                               <  1:731918‑M1/54‑1 (MQ=255)
                                                 gCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAATAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcacctatatttccagacatctgttatcacttaaccaattac                                                                                               >  2:493004‑M1/1‑94 (MQ=255)
                                                   tctccaatgactagtctgaaagcgagtattgagactatcccttatttaagtgatattggttgtctggagtttcaggcggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGAttt                                                                                             >  2:907047‑M1/88‑143 (MQ=255)
                                                    ctccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTg                                                                                            <  1:469116‑M1/57‑1 (MQ=255)
                                                     tccaatgactagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTATTACTTAGCGTAATCGGATTTgg                                                                                           <  2:34600‑M1/58‑1 (MQ=255)
                                                      tAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcc                                                                                          >  2:479754‑M1/1‑89 (MQ=255)
                                                           aTTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTATTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctg                                                                                     <  1:547343‑M1/143‑60 (MQ=255)
                                                             ctagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAatat                                                                                   <  2:330127‑M1/66‑1 (MQ=255)
                                                              tagtctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATAtt                                                                                  <  2:737642‑M1/67‑1 (MQ=255)
                                                               agtctaaaaactcgtattaagactatcacttagttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGCCCTTTTACTTCACGTAATCCGGTTTGGCTGTATAttt                                                                                 <  2:28140‑M1/68‑1 (MQ=255)
                                                                tATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAGGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaaccccgctgccgcg                                                                                >  1:329844‑M1/1‑79 (MQ=255)
                                                                 tctaaaaactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTa                                                                               <  1:949211‑M1/70‑1 (MQ=255)
                                                                 tctaaaaactagcattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTa                                                                               >  1:701971‑M1/74‑143 (MQ=255)
                                                                 gagcaACAACTGAAAAAAAATTACAACAAGGGCTTGCTGAACTGAACAAAACAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcag                                                                               <  2:207161‑M1/141‑66 (MQ=255)
                                                                       caacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattc                                                                         <  1:755446‑M1/143‑72 (MQ=255)
                                                                       aactagtattaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAgccgcc                                                                         <  1:296123‑M1/76‑1 (MQ=255)
                                                                        aacTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGCGTAAAGTTAGGGGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcc                                                                        >  1:279754‑M1/1‑71 (MQ=255)
                                                                           tGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgt                                                                     <  2:317025‑M1/143‑76 (MQ=255)
                                                                            gAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtg                                                                    <  2:139773‑M1/143‑77 (MQ=255)
                                                                             aaaaaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtgg                                                                   >  2:393023‑M1/1‑66 (MQ=255)
                                                                               ttaagactatcacttatttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCcagtcag                                                                 <  1:713252‑M1/84‑1 (MQ=255)
                                                                                aaaaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgccccgatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcga                                                                <  2:510536‑M1/143‑81 (MQ=255)
                                                                                  aaaTTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgcccccgatattcccgtggcgagc                                                              >  1:378328‑M1/1‑61 (MQ=255)
                                                                                   gaccatgacttatttgagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAAt                                                             >  1:363949‑M1/56‑143 (MQ=255)
                                                                                       atcacttatttaagtcatattggttgtctggagattcagggggccagtctaCGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACt                                                         >  1:452276‑M1/52‑143 (MQ=255)
                                                                                        tcacttatttaagggatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCATTactgactg                                                        >  1:244831‑M1/51‑143 (MQ=255)
                                                                                         aaaaaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacc                                                       >  2:570621‑M1/1‑54 (MQ=255)
                                                                                           cttatttaagttattatggttgtctggagattccgggggccagtctaGGTATAGATGTAAGACGACCTATCGTTCAGACCGTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTgggg                                                     >  2:486994‑M1/48‑143 (MQ=255)
                                                                                            aaGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccaggcatctgttatcacttaaccaattattagcgttctgccgcagatattcccgtggcgagcgataacccag                                                    >  1:85005‑M1/1‑51 (MQ=255)
                                                                                              atttaagtgatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGt                                                  <  1:310701‑M1/99‑1 (MQ=255)
                                                                                                  tttCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataaccccgcgacct                                              >  1:473085‑M1/1‑45 (MQ=255)
                                                                                                    gtgatatccgtcgtctggagatgcaggtggtcagtctaGGGACAGAGGTAAGTTGTTCTATCTTTCAGACCTTTCACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTa                                            >  2:317575‑M1/39‑143 (MQ=255)
                                                                                                      tgaactgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgc                                          <  1:668966‑M1/143‑103 (MQ=255)
                                                                                                      gatattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTAtt                                          <  2:451938‑M1/107‑1 (MQ=255)
                                                                                                        tattggttgtctggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGCCTGAATATTCTATCCGTCTCAGTCAGTAATGACTGGGGCGTTTTTTATTgg                                        >  1:966240‑M1/35‑143 (MQ=255)
                                                                                                          ctgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggat                                      <  1:121998‑M1/143‑107 (MQ=255)
                                                                                                          ctgaacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaaccaattacaagcccgctgccgcagatcttcccgtggcgagcgataaccccacgcactatgcggac                                      >  2:260518‑M1/1‑37 (MQ=255)
                                                                                                             aacAAAAAAGAGTAAAGTTAGTCGCGTGGGGTACtggatttgcccctatattcccagacatctgttatcactcaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgcc                                   <  2:688983‑M1/143‑110 (MQ=255)
                                                                                                             aacAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcaagcgataacccagcgcactatgcggatgcc                                   >  1:646589‑M1/1‑34 (MQ=255)
                                                                                                                  aaaaGAGTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagctattcccgtggcgagcgataaccccgcgcactacgcggacgcccttcg                              >  2:77686‑M1/1‑29 (MQ=255)
                                                                                                                   tggggattccgggggccagcctaGGTACAGAGGTGAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                             >  2:270136‑M1/24‑143 (MQ=255)
                                                                                                                   tggagattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTGACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGaaagaaaa                             >  2:498402‑M1/24‑143 (MQ=255)
                                                                                                                      agattcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGAt                          >  1:418604‑M1/21‑143 (MQ=255)
                                                                                                                       agTAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactgtgcggatgccactcgttata                         >  1:784925‑M1/1‑24 (MQ=255)
                                                                                                                         ttcagggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGCCCTTTTACTTCACGTAATCGGAGTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCg                       >  1:69794‑M1/18‑143 (MQ=255)
                                                                                                                         tAAAGTTAGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataccccagcgcaccacgcggctgcccttcgctacaat                       >  2:659882‑M1/1‑22 (MQ=255)
                                                                                                                          aacgtgagtcgcgTAGGGTACtggatttgcccctatatttccagacatctgttatcacttagcccattaccagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatg                      <  2:764142‑M1/137‑123 (MQ=255)
                                                                                                                             gggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAAt                   >  1:191399‑M1/14‑143 (MQ=255)
                                                                                                                             gggggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAAt                   >  1:794550‑M1/14‑143 (MQ=255)
                                                                                                                               gggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGc                 >  1:90949‑M1/12‑143 (MQ=255)
                                                                                                                               gggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGGTTTTTATTGGGCGACAGAAAAGATCCGTACTGc                 <  2:374327‑M1/132‑1 (MQ=255)
                                                                                                                                ggccagtctaGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGcc                >  2:420352‑M1/11‑143 (MQ=255)
                                                                                                                                aGTCGCGTAGGGTACtggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaa                <  1:886889‑M1/143‑129 (MQ=255)
                                                                                                                                       ctaGGGACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATgcg         >  1:51080‑M1/4‑143 (MQ=255)
                                                                                                                                         aGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCt       >  2:469116‑M1/2‑143 (MQ=255)
                                                                                                                                          ggTACAGAGGTAGGATGTTCTATCTTTCACTCCTCGTACCTCACGTAGTCGGCTTCGGCTGAATATTTCGGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTCTAGTACGAAAGAAAAGATCCGTAATGCCTGATGCGCTa      <  2:258326/143‑1 (MQ=255)
                                                                                                                                            tACtggatttgcccctctcctcccacacatctgttatcacttaacccagtacgagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaag    <  2:402937‑M1/143‑141 (MQ=255)
                                                                                                                                             aCtgtatttccccctagctttccagacatctgatatcacgtaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaagg   <  2:752724‑M1/143‑142 (MQ=255)
                                                                                                                                              ctggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgccgcagatattcccgtggcgagcgataacccagcgcactatgcggatgccattcgttataatgctcgaacgcctctgcaaggt  >  1:414893‑M1/1‑1 (MQ=255)
                                                                                                                                              ctggatttgcccctatatttccagacatctgttatcacttaacccattacaagcccgctgcagcggatattcccgtggcgagcgataacccagcgcactatgcggagcccattcgttagaaagctcgaacgcccctgcacggt  >  2:236316‑M1/1‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CATGCCTTTTTTCGTAAGTAAGCAACATAAGCTGTCACGTTTTGTGATGGCTATTAGAAATTCCTATGCAACAACTGAAAAAAAATTACAAAAAGTGCTTTCTGAACTGAACAAAAAAGAGTAAAGTTAGTCGCGTAGGGTACAGAGGTAAGATGTTCTATCTTTCAGACCTTTTACTTCACGTAATCGGATTTGGCTGAATATTTTAGCCGCCCCAGTCAGTAATGACTGGGGCGTTTTTTATTGGGCGAAAGAAAAGATCCGTAATGCCTGATGCGCTATGTT  >  NC_000913/4049765‑4050049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.