Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499	1300697–1299500	2–1199	83 [0]	[0] 83	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	82 (1.500)	63/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299360‑1299640                                                                                                                                           |                                                                                                                                               tACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCATTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                            >  2:294555‑M1/1‑139 (MQ=255)     gttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                                                                        <  1:911881‑M1/143‑9 (MQ=255)         gttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                    <  2:129457‑M1/143‑13 (MQ=255)          ttgtaaggATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  2:388773‑M1/140‑14 (MQ=255)          ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGTGCTTGCTGAAGAATAATTGAAATGATATTATTAATGCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                   <  1:457451‑M1/143‑14 (MQ=255)             taAGGATTTCCTTAAATGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                >  2:373563‑M1/1‑127 (MQ=255)             taAGGATTTCATTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                >  1:1014632‑M1/1‑127 (MQ=255)               aagatTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                              <  1:254754‑M1/141‑19 (MQ=255)               aGGATTTCCTGAACGGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATTATATTAGGAATTACACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                              <  2:215003‑M1/143‑19 (MQ=255)                     tCCTTAACTGCTTCTCCTCTCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                        <  1:889055‑M1/143‑25 (MQ=255)                     tCCCTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                        <  2:12076‑M1/143‑25 (MQ=255)                       cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattcttgt                                                                                                                      >  2:1008253‑M1/1‑117 (MQ=255)                             tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                >  2:187872‑M1/1‑111 (MQ=255)                               cTTCTCCTCACCATCATGTTCTTTTCGTCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                              <  2:929831‑M1/143‑35 (MQ=255)                                ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGGAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGATGAAAGTGCTAAataataatcaattgttaaattattgtgcattccac                                                                                                             >  1:564885‑M1/1‑108 (MQ=255)                                  ctcctcACCATCATGTTATTTTCGCCACATCATAATCCGGGGCTTGAACTAGGATTATTTAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                           <  1:737834‑M1/143‑38 (MQ=255)                                  ctcctcACCATCATGTTATCTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGACGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                           <  1:2197‑M1/143‑38 (MQ=255)                                   tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAActaataatcaattgttaaattattgtgcatttaactac                                                                                                          >  2:93796‑M1/1‑105 (MQ=255)                                   tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactac                                                                                                          >  2:753872‑M1/1‑105 (MQ=255)                                      tctccatcATATTATTTTCGCCACATCATAATCCTGGGCTTGCTTAATAATAATTGAAATGATATTATTAATTCCACTTCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                       <  2:660818‑M1/140‑42 (MQ=255)                                      tcACCATCATTTTATTTTCGCCACATCATAATCCTAGTCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGATGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                       <  2:660815‑M1/143‑42 (MQ=255)                                       cACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgga                                                                                                      <  2:1001012‑M1/143‑43 (MQ=255)                                        aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  1:163289‑M1/143‑44 (MQ=255)                                        aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                     <  1:545932‑M1/143‑44 (MQ=255)                                          catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaact                                                                                                   <  1:468941‑M1/143‑46 (MQ=255)                                            tcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                 >  1:366982‑M1/1‑96 (MQ=255)                                            tcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                 >  1:300916‑M1/1‑96 (MQ=255)                                              atGTTATTTTCGCCACATCATAAGCCTGGGCTGGCTGAAGAATAATTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataacaatcaattgttaaattattgtgcatttcactactggaactgtaa                                                                                               <  1:978639‑M1/143‑50 (MQ=255)                                              acgttATTTTCGCTACATCATAATCCTGGGCTAGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCGTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaagtattgtgcatttcactactggaactgtaa                                                                                               <  1:393494‑M1/141‑50 (MQ=255)                                               tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAGAGTGCTAAataataatcaattgttaaattattgtgaatttaactactggaagtgtaat                                                                                              >  2:138534‑M1/1‑93 (MQ=255)                                                 ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactataatca                                                                                            >  2:261219‑M1/1‑91 (MQ=255)                                                   aTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGCTATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTCAataataatcaattgttaaattattgtccatttcactactggcactgtaatccga                                                                                          >  1:933789‑M1/1‑89 (MQ=255)                                                      ttCGTCAGATCATAATCCTGGGCGTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                       <  1:845603‑M1/143‑58 (MQ=255)                                                      ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                       <  1:77895‑M1/143‑58 (MQ=255)                                                      ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAAGTCCACTGCATTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatctcactactgggactgtaatcggaacg                                                                                       >  2:65217‑M1/1‑86 (MQ=255)                                                       tCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattatgcatttaactactggaacagtaatcagaaaag                                                                                      >  2:833622‑M1/1‑85 (MQ=255)                                                          ccACATCATAATCCTGGGCTTGCTGAAGAATGATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaactgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                   <  2:985963‑M1/143‑62 (MQ=255)                                                          ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                   <  2:584185‑M1/143‑62 (MQ=255)                                                            acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttatattattgtgcatttgcctactggacctgtaatcagaaaagatagt                                                                                 >  1:374220‑M1/1‑80 (MQ=255)                                                            acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                 <  1:862695‑M1/143‑64 (MQ=255)                                                                catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                             >  2:579872‑M1/1‑76 (MQ=255)                                                                 atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgc                                                                            >  1:382663‑M1/1‑75 (MQ=255)                                                                   aaTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctt                                                                          >  1:625718‑M1/1‑73 (MQ=255)                                                                    aTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                         <  1:343343‑M1/143‑72 (MQ=255)                                                                     tcttgggCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactaatggaactgtaatcagaaaagatagacatgcttag                                                                        <  2:20116‑M1/140‑73 (MQ=255)                                                                              tGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcaaatctcc                                                               >  1:399688‑M1/1‑62 (MQ=255)                                                                               gCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctcta                                                              <  1:222201‑M1/143‑83 (MQ=255)                                                                                cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                             <  1:849295‑M1/143‑84 (MQ=255)                                                                                 tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGGGGAAAGTGCTAAttaataatcaattgttaaattattgtgcatttgactaatggcactgtaatcagaaaagatagacatgcatagccaatatcaatt                                                            >  2:313576‑M1/1‑59 (MQ=255)                                                                                      aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                       >  2:119621‑M1/1‑54 (MQ=255)                                                                                      aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                       >  1:863099‑M1/1‑54 (MQ=255)                                                                                           ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgactt                                                  >  1:839586‑M1/1‑49 (MQ=255)                                                                                            tGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgacttg                                                 >  1:428678‑M1/1‑48 (MQ=255)                                                                                               aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                              <  2:981029‑M1/143‑99 (MQ=255)                                                                                                  gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttccctactggaactgtaatccgaaaagatagacatactcagccaatatctatttgaatgaattgaaagat                                           >  1:532884‑M1/1‑42 (MQ=255)                                                                                                   acattattaaTTCCACTGGCTTTAGTAGAGGAAAGTGCTAAataataatcaattggtaaatgattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatg                                          <  1:944122‑M1/141‑103 (MQ=255)                                                                                                      ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                       >  2:700108‑M1/1‑38 (MQ=255)                                                                                                      ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                       <  1:198180‑M1/143‑106 (MQ=255)                                                                                                      ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatgtgagtgagttgaaagatgttg                                       >  1:493475‑M1/1‑38 (MQ=255)                                                                                                         ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                    >  1:947374‑M1/1‑35 (MQ=255)                                                                                                           aaTTCCACTGCCTTTGGTAGAGGAAAGTTCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                  <  1:982412‑M1/143‑111 (MQ=255)                                                                                                           aaTTCCACTGCCTTCGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaa                                  <  1:286397‑M1/143‑111 (MQ=255)                                                                                                            aTTCCACTGCCTTTGGTAGAGTAAAGTGCTAAataataatcaattgttaaattattgtggatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 <  2:503633‑M1/143‑112 (MQ=255)                                                                                                            aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 <  1:751132‑M1/143‑112 (MQ=255)                                                                                                            aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaag                                 >  2:997768‑M1/1‑32 (MQ=255)                                                                                                             ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                                >  1:377632‑M1/1‑31 (MQ=255)                                                                                                              cccaCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggc                               <  1:950818‑M1/142‑114 (MQ=255)                                                                                                                  cggccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                           <  1:178180‑M1/141‑118 (MQ=255)                                                                                                                    gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                         <  1:753872‑M1/143‑120 (MQ=255)                                                                                                                     ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatg                        >  2:808673‑M1/1‑23 (MQ=255)                                                                                                                         tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                    >  2:108627‑M1/1‑19 (MQ=255)                                                                                                                          ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggagctgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                   <  1:764255‑M1/143‑126 (MQ=255)                                                                                                                            gagaggAGAGTGCTAAacaataatcaattgttaaattagtgtgcgtttcgctactggaactgtaatcagaaaagatagacaggcttagccaatcgctatgtgatggaattgaaagatgtttgttaaggcatggatgcaagcta                 <  2:169309‑M1/142‑128 (MQ=255)                                                                                                                             agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                <  1:119621‑M1/143‑129 (MQ=255)                                                                                                                             agagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                <  1:100950‑M1/143‑129 (MQ=255)                                                                                                                              gagGAAAGTGCTAAataataatcaattgttgtgtgagtgtccaattcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata               <  2:444479‑M1/143‑130 (MQ=255)                                                                                                                              gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata               >  1:727303‑M1/1‑14 (MQ=255)                                                                                                                              gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagaagtttgttaaggcatggatgcaagctata               >  2:141178‑M1/1‑14 (MQ=255)                                                                                                                                ggAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctataga             <  1:147628‑M1/143‑132 (MQ=255)                                                                                                                                    aGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattct         <  2:947374‑M1/143‑136 (MQ=255)                                                                                                                                      tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgttagaagtttgttaaggcatggatgcaagctatagattctga       >  2:209802‑M1/1‑6 (MQ=255)                                                                                                                                      tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga       >  1:678144‑M1/1‑6 (MQ=255)                                                                                                                                           aataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcacggatacaagctatagattatgatacgg  >  2:122356‑M1/1‑1 (MQ=255)                                                                                                                                           |                                                                                                                                               TACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTT  >  NC_000913/1299360‑1299640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑