Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258675 258683 9–776 68 [0] [0] 68 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)65 (1.220) 52/268 0.0 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTT  >  NC_000913/258542‑258823
                                                                                                                                              |                                                                                                                                           
tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaagatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                             <  1:656533‑M2/1‑1 (MQ=255)
tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                             <  1:675513‑M2/1‑1 (MQ=255)
   taaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                                                          >  2:256133‑M2/140‑143 (MQ=255)
     aaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCa                                                                                                                                        <  1:45073‑M2/6‑1 (MQ=255)
      aaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggccaggatattcgtgaaggtaAGTGCaa                                                                                                                                       >  2:628469‑M2/137‑143 (MQ=255)
              taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgcagcgctgattttgttaaacatttacggcactaggcgcgtatagtcgtgcaggtaAGTGCAAAGATAATc                                                                                                                               >  1:375762‑M2/129‑143 (MQ=255)
              taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                               >  2:318202‑M2/129‑143 (MQ=255)
              taaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                               >  1:777917‑M2/129‑143 (MQ=255)
               aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaggagcagattgatcaaaaaatttaccggactaggcccgtatattcgtgaaggtaAGTGCAACGATAATCg                                                                                                                              >  1:549993‑M2/128‑143 (MQ=255)
               aaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgaccttaccgagtggacacccgaagagcagattgatccaaaaatttaccgcactaggcccctatattcgttaaggtaAGAGCAAAAATAATCg                                                                                                                              >  1:432436‑M2/128‑143 (MQ=255)
                  ttttgccaatttggtataacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAtt                                                                                                                           <  1:250167‑M2/19‑1 (MQ=255)
                   tttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                          <  1:830005‑M2/20‑1 (MQ=255)
                   tttgcaaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                          <  1:369713‑M2/20‑1 (MQ=255)
                        caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                     <  1:884219‑M2/25‑1 (MQ=255)
                        caattcggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatgctccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                     <  1:469332‑M2/25‑1 (MQ=255)
                         aattttgtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                    <  2:632857‑M2/26‑1 (MQ=255)
                         aatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaagggaAGTGCAAAGATAATCGATTCTTTTTc                                                                                                                    >  1:288582‑M2/118‑143 (MQ=255)
                          tgtttgtaaaacagtcgcatcccaacaggagatcgcaatgacgttaccgagtggacaccccaagagcagattgatcaaaaaatgtaccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                   <  2:799152‑M2/27‑1 (MQ=255)
                          atttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCg                                                                                                                   <  1:789352‑M2/27‑1 (MQ=255)
                           tctggtaaaacagctgcaccacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                  <  1:76766‑M2/28‑1 (MQ=255)
                             tggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAtt                                                                                                                <  2:143394‑M2/30‑1 (MQ=255)
                                taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgacggtaAGTGCAAAGATAATCGCTTCTCTTTCGATTGTc                                                                                                             >  2:1013811‑M2/111‑143 (MQ=255)
                                taaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                             <  1:40363‑M2/33‑1 (MQ=255)
                                    acagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGc                                                                                                         <  1:105871‑M2/37‑1 (MQ=255)
                                    acagttgcatcacaacaggagatagcaatgacgttaccgagcggacacccgaagagcagagtgatcaaaaaatttaccgcacccggcccgtaaattcgggaaggaaAGTGCAAAGATAATCGCGTCCTGTTCGATtggctggc                                                                                                         >  2:693860‑M2/107‑143 (MQ=255)
                                     ccgttgcatcacaataggagatagcaatgaccttacctagtcgacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                        <  2:351181‑M2/38‑1 (MQ=255)
                                           catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaagtttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                  <  2:185598‑M2/44‑1 (MQ=255)
                                               gcaacaggagatggcgatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCa                                                                                              <  2:50768‑M2/48‑1 (MQ=255)
                                                      gagattgcaatgactataccgaggggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                                                                       <  1:133865‑M2/55‑1 (MQ=255)
                                                             cactgacgtttccgaggggacacccgaagggcagattgatcaacaaatattccgcatgaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCa                                                                                <  2:878070‑M2/62‑1 (MQ=255)
                                                             caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGACACCGGCa                                                                                >  2:218818‑M2/82‑143 (MQ=255)
                                                              aaggacgttaccgtgtggacagccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                                                               <  2:665449‑M2/63‑1 (MQ=255)
                                                                   cgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                          <  1:814183‑M2/68‑1 (MQ=255)
                                                                   cgtgacggaggggacacccgaagagcagattgatcaaaaaatttaccgcggtaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGaa                                                                          <  1:790575‑M2/68‑1 (MQ=255)
                                                                    tttgccgagaggacacccgaagggcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                         <  1:898622‑M2/69‑1 (MQ=255)
                                                                        ccgagtggacacccgaagagcagattgaccaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCg                                                                     <  1:13260‑M2/73‑1 (MQ=255)
                                                                         cgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtctattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                    >  2:516929‑M2/70‑143 (MQ=255)
                                                                                acacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCGACGTGAAACCGGCACCGGAAGTGCGTGAATTCt                                                             >  1:828942‑M2/63‑143 (MQ=255)
                                                                                 cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                            <  1:933329‑M2/82‑1 (MQ=255)
                                                                                 cacccgaagagaagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                            <  1:869625‑M2/82‑1 (MQ=255)
                                                                                  acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                           <  1:560699‑M2/83‑1 (MQ=255)
                                                                                   ccgtacgagcagattgatcaaaaaatttacctcactaggctcgtatattcgtgaaggtaAGTGCAAAGATAATCGTTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGTAACCGGCACCGGAAGTGCGTGAATTCTggg                                                          <  2:85946‑M2/84‑1 (MQ=255)
                                                                                   cccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTggg                                                          <  2:487985‑M2/84‑1 (MQ=255)
                                                                                    ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                         >  2:619225‑M2/59‑143 (MQ=255)
                                                                                    ccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                         <  2:570118‑M2/85‑1 (MQ=255)
                                                                                     cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGCAGTGCGTGAATTCTGGGGc                                                        >  2:930272‑M2/58‑143 (MQ=255)
                                                                                        agagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGACACCGGAAGTGCGTGAATTATGGGGCtgg                                                     >  1:834298‑M2/55‑143 (MQ=255)
                                                                                          cgcagagtggtcaaaaaatttaccgcactgggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTGTGCGATTGTCTGGCTGTATGCGTCAACGTGAGACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                   <  2:84515‑M2/91‑1 (MQ=255)
                                                                                          agcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtg                                                   <  1:747695‑M2/91‑1 (MQ=255)
                                                                                           gcagattgatcaaaaaattgaccgcgctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                  <  1:87096‑M2/92‑1 (MQ=255)
                                                                                               attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg                                              <  1:701844‑M2/96‑1 (MQ=255)
                                                                                                     caaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTgg                                        >  1:580205‑M2/42‑143 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgacggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTACGGCTGCATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCtggc                                       >  1:331428‑M2/41‑142 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGa                                       <  1:256133‑M2/103‑1 (MQ=255)
                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGa                                       >  2:741796‑M2/41‑143 (MQ=255)
                                                                                                          aatgtaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGTGGCTGGTGGATGGAGCTGGAAgcg                                   <  1:449330‑M2/107‑1 (MQ=255)
                                                                                                            ttttccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAGTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCa                                 <  1:106973‑M2/109‑1 (MQ=255)
                                                                                                              taccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAgg                               <  1:318202‑M2/111‑1 (MQ=255)
                                                                                                                       agtcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                      <  1:506048‑M2/120‑1 (MQ=255)
                                                                                                                       aggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                      <  1:596138‑M2/120‑1 (MQ=255)
                                                                                                                        ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGttt                     <  1:619225‑M2/121‑1 (MQ=255)
                                                                                                                           ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTAAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                  >  1:392473‑M2/20‑143 (MQ=255)
                                                                                                                              tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTa               <  1:402455‑M2/127‑1 (MQ=255)
                                                                                                                                gtttcgtgaaggtaCGGGCAAAGATAATCGATTCTGTTTCGATTGTCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa             <  2:695559‑M2/129‑1 (MQ=255)
                                                                                                                                     gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGCATCCCGTTTTACCTACAGTTAc        >  1:360321‑M2/10‑143 (MQ=255)
                                                                                                                                        aaggtaAGTGCAAATATAATCGTGTCTTTTTCGATTGTTGGGCTGTATGCGGCAACGGGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAg     <  1:222361‑M2/137‑1 (MQ=255)
                                                                                                                                           gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGGTGGCGCTGGACGCGCCGGGATCCCGTTTTACCTGCagtttccgattt  >  1:437048‑M2/4‑138 (MQ=255)
                                                                                                                                           gtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt  >  1:527941‑M2/4‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                           
GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTT  >  NC_000913/258542‑258823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: