Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,305	C→T	R395C (CGT→TGT)	icd →	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,305	0	C	T	100.0%	173.5 / NA	60	R395C (CGT→TGT)	icd	isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/27);  total (33/27)

GGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCT  >  NC_000913/1196166‑1196434                                                                                                                                            |                                                                                                                                  ggACAAAGTAGAGCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTc                                                                                                                                <  1:942410/143‑1 (MQ=255)  gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCt                                                                                                                               >  1:793911/1‑143 (MQ=255)  gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGCTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCt                                                                                                                               >  2:939460/1‑143 (MQ=255)    cAATGTAACTCCCGGCTCTATTATGCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTga                                                                                                                             <  1:485259/143‑1 (MQ=25)     aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGACGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgat                                                                                                                            >  1:363642/1‑143 (MQ=255)     aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGGGTGTCTgat                                                                                                                            >  1:1017687/1‑143 (MQ=255)        gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGCCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGCAAAGCGCACCCTATGACCGCGGGTCTCTgatgga                                                                                                                         >  2:433687/1‑143 (MQ=25)        gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATCGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTCTCTgacgga                                                                                                                         >  2:190167/1‑143 (MQ=255)          cagtCCTGGCTCTATTATGCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatggatg                                                                                                                       <  2:735103/140‑1 (MQ=255)             tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGTACCGAAGCGGCTGACTTCATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAGCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                    >  2:52214/1‑143 (MQ=255)             tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                    <  1:118713/143‑1 (MQ=255)             tCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcg                                                                                                                    <  1:305550/143‑1 (MQ=255)                  gCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTaaa                                                                                                               >  1:195151/1‑143 (MQ=255)                     ctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAActg                                                                                                            >  2:964108/1‑143 (MQ=255)                            tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAAt                                                                                                     <  1:719925/143‑1 (MQ=255)                              tctcCGCTGAGATGATGCTGCGCCACTTGGGTTGGACCGACGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGCTGGCTGGCCGTACACTGCTGAAATGt                                                                                                   >  2:363199/1‑143 (MQ=21)                               cgccgccgCGATGATGCTGCGCCGCATGGGTTGGACCGAAGCGGCTGCCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGtt                                                                                                  <  2:212184/141‑1 (MQ=18)                                tcCGCTGGGATGATGCTGCGCCCCATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTc                                                                                                 <  1:111037/143‑1 (MQ=255)                                 ccGCGGAGATGATGCTGCGCCACATGGGTTGGACCGCAGCGGCTGACCTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa                                                                                                <  2:483932/143‑1 (MQ=255)                                     ctaactaatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGt                                                                                            >  1:379764/8‑143 (MQ=255)                                       aactaatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt                                                                                          <  2:61771/138‑1 (MQ=255)                                         atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTgg                                                                                        <  2:556815/143‑1 (MQ=255)                                         atgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTgg                                                                                        <  1:634763/143‑1 (MQ=255)                                          tgatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt                                                                                       <  1:1000908/143‑1 (MQ=255)                                            atgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa                                                                                     <  1:940130/143‑1 (MQ=255)                                                   gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatc                                                                              <  1:1016536/143‑1 (MQ=255)                                                    ccACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca                                                                             >  1:569643/1‑143 (MQ=255)                                                                gACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAGACATGt                                                                 >  1:922697/1‑143 (MQ=255)                                                                gACCGAAGCGGCTGACTTAATTGTTAAAGGGATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt                                                                 <  1:303334/143‑1 (MQ=255)                                                                 aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTa                                                                >  1:675290/1‑143 (MQ=255)                                                                            tcaCTTAACTGTCAAAGGTATCGAACGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt                                                     <  1:479810/141‑1 (MQ=18)                                                                            tGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt                                                     >  1:161832/1‑143 (MQ=255)                                                                               cTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGCAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGCTCAGAGCGCGGTGACGCGATCACCGAAAACATGTAATGCCGTAGTTTgc                                                  >  2:446574/1‑142 (MQ=18)                                                                               cTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGCTCATCGACAACATGTAATGCCGTAGTTTGt                                                  >  1:752023/1‑143 (MQ=255)                                                                                ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGACAACATGTAATGCCGTAGTTTGtt                                                 >  1:289330/1‑143 (MQ=255)                                                                                ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTATTGCCGTAGTTCGtt                                                 >  1:86126/1‑143 (MQ=255)                                                                                ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt                                                 >  2:320648/1‑143 (MQ=255)                                                                                 tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAGAACATGTAATGCCGTAGTTTGTTa                                                >  2:757202/1‑143 (MQ=255)                                                                                 tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa                                                >  1:638981/1‑143 (MQ=255)                                                                                  aaTTGTTAAAGGGATGGAAGGCGCAGTCAACGCGAAAACCGTAACTTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTaa                                               <  2:412408/143‑1 (MQ=255)                                                                                      gTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAttt                                           >  1:46931/1‑143 (MQ=255)                                                                                        tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAACACATGTAATGCCGTAGTTTGTTAAAtgtct                                         >  2:586539/1‑139 (MQ=255)                                                                                             gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAGTTATTAACg                                    >  2:189700/1‑143 (MQ=255)                                                                                                  gAAGGCGCAAACAGGGCGACATACGGAACCGATGACGTGGAGTGTCTGATGGATGGCGCTAAACTGCTGAATTGTTCAGAGTTTGGTTGCTCGATGATCGAAAGCATGTAATGCCTTAGTTTGTTAAATTTATTAACGGGAGc                               <  2:368468/143‑1 (MQ=255)                                                                                                       cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAAc                          <  1:870607/143‑1 (MQ=255)                                                                                                        gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAAAGTTTGGTGACGCGATAATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACg                         <  1:581591/143‑1 (MQ=255)                                                                                                             cAACGCGAAAACCGGAACCTATGTCTTCGAGTGTCCGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGCCGCGATCATCGAAACCCTGTAATGCCGTAGTTTGTTCAATTTATTAACGGGAGCGTAACGCTccc                    <  2:203369/143‑1 (MQ=25)                                                                                                              aaCGCGATACCCTTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAGCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTTACGCTCCCg                   <  2:293020/143‑1 (MQ=25)                                                                                                              aaCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGGTTGTTAAATGTATTAACGGGAGCGTAACGCTctag                   >  2:260134/1‑140 (MQ=255)                                                                                                               aCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGCACTGTTCAGAGGTTGGTGACGCGCTCCTCGAAAACATGTAATGCCGTAGTGTGTTAAACTTATTACCGGCACCCTACCGCTCCCgt                  >  2:3358/1‑143 (MQ=255)                                                                                                                cgcgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgtt                 >  1:317110/1‑143 (MQ=255)                                                                                                                cgcgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgtt                 <  1:79256/143‑1 (MQ=255)                                                                                                                   gAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGGTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAGTTTATTAACGGGAGCGTAACGCTCCCgttgtt              >  2:751253/1‑143 (MQ=255)                                                                                                                   gAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAAATGCTGAAATGTTCGGAGTTGGGTGAGGCCATCATAGAAAACATGTAATGCCGTAGATTGTTAAATCTATTAACGAGCGCGTAAAGCTCTAgttgtt              >  2:766334/1‑143 (MQ=255)                                                                                                                       aCCGTAACCTATGGCTTCGAGTGTCTGATGGATGGCGCTACGCGGCTGGAATGTTCAGAGTTTGGTGACGCGAGCATCGAACACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt          <  2:64984/143‑1 (MQ=25)                                                                                                                        ccGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTg         <  1:695657/143‑1 (MQ=255)                                                                                                                         cGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGGTTGGTGACGCGATGATCGAAGACATGTAATGCCGTAGTTTGTTAGATTTATTGATGGGAGCGTACTGCTCCCGTTGTTTTTTGt        >  1:810088/1‑143 (MQ=255)                                                                                                                          gTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGtt       <  1:964108/143‑1 (MQ=255)                                                                                                                          gTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAAGGTTCAGAGTTTGGTGACGCGATCATCGAAAACAAGTAATGCCGTAGTTTCTTCACTCATTTATCGGGACCGTAACGCTCCCGTTGttttttctg       >  2:982607/1‑140 (MQ=255)                                                                                                                               cTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgct  >  1:251831/1‑143 (MQ=255)                                                                                                                                            |                                                                                                                                  GGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCT  >  NC_000913/1196166‑1196434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑