BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	353,606	A→G	14.0%	M301V (ATG→GTG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,606	0	A	G	14.0%	246.5 / 4.1	107	M301V (ATG→GTG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (35/52); new base G (14/3); total (50/57)
Fisher's exact test for biased strand distribution p-value = 2.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATG  >  NC_000913/353369‑353843

    gggTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCg                                                                                                                                                                                                                                                 >  1:141750/1‑239 (MQ=255)

      gTACCGGGGGCGTGGCTGGAATCCAACGAACCCTCCTGCATTCTCTACCCTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGa                                                                                                                                                                                                                                                <  2:1069642/238‑1 (MQ=255)

           ggTGGCGTGGCTTGAACCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACGCCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTa                                                                                                                                                                                                                                             <  2:924741/236‑1 (MQ=255)

                          agccAACGAAACCTCCTGCATTCTCTACGCGTCCGGCACGACCGGCAAACCGAAAGGCGTGCATCGTGACGTCGGCGGATATGCGGTGGCGCGGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                                                                                                                                                                                                            <  2:1184284/236‑1 (MQ=255)

                          aTCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTg                                                                                                                                                                                                                            <  2:1210350/238‑1 (MQ=255)

                                      ctcctGCATTCTCTACCCTTCCGGCCCGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑gg                                                                                                                                                                                                             <  2:1148307/239‑1 (MQ=255)

                                       tcctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGa                                                                                                                                                                                                            <  2:489532/239‑1 (MQ=255)

                                        cctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑gg                                                                                                                                                                                                             >  1:1174181/1‑237 (MQ=255)

                                         ctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACt                                                                                                                                                                                                          <  2:549450/239‑1 (MQ=255)

                                         ctGCATTCTCTACACTGCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACt                                                                                                                                                                                                          <  1:1305995/239‑1 (MQ=255)

                                              ttCTCTACACCTCCGGCGCGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTgcggc                                                                                                                                                                                                     <  1:1256116/239‑1 (MQ=255)

                                              ttCACTACACTTCCGGCACGACTGGCAAACCTAAAGGCGTGCAGCGGGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTgcggc                                                                                                                                                                                                     <  1:487776/239‑1 (MQ=255)

                                                 tctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCgtg                                                                                                                                                                                                  <  1:216694/239‑1 (MQ=255)

                                                     cacTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTgtggt                                                                                                                                                                                              >  1:448966/1‑239 (MQ=255)

                                                          ccGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCCATCGTTTACGAAGGATTGCCGACCCGGCCC‑GGAC‑gcggcggggtggggaca                                                                                                                                                                                         >  2:804600/1‑228 (MQ=255)

                                                          ccGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGGGgggtgggcc                                                                                                                                                                                         >  1:38526/1‑236 (MQ=255)

                                                           cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTATCGTTTACGAAGGATTGCCGACCCGG‑CC‑GGACTGCGGCGTGTGGTGGGCaa                                                                                                                                                                                        >  1:1241709/1‑239 (MQ=255)

                                                           cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTATCGGTTACGAAGGATTGCCGCACCGG‑CC‑GGACCGCGGGGTGTGGGGGaaaa                                                                                                                                                                                        >  1:1124115/1‑239 (MQ=255)

                                                               aCGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATAGGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAAtcgt                                                                                                                                                                                    <  2:950028/239‑1 (MQ=255)

                                                                 gACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTC‑TTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCga                                                                                                                                                                                 <  1:900224/239‑1 (MQ=255)

                                                                   ccGGCAACCCTAAAGGCGTGCAGGGTGACGTCGGCGGATATGCGGTGGCGCTGGCGTCCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCga                                                                                                                                                                                 <  2:1341461/238‑1 (MQ=255)

                                                                   ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCgag                                                                                                                                                                                <  2:755558/239‑1 (MQ=255)

                                                                   ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCgag                                                                                                                                                                                <  2:141750/239‑1 (MQ=255)

                                                                           ccTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACg                                                                                                                                                                                                                                    <  1:182155/181‑1 (MQ=255)

                                                                            cTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCgtg                                                                                                                                                                                                  <  1:376749/212‑1 (MQ=255)

                                                                             tAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGGCACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAgg                                                                                                                                                                     <  1:640773/239‑1 (MQ=255)

                                                                               aaGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑t                                                                                                                                                                   <  2:181601/238‑1 (MQ=255)

                                                                                aGGCGTGCAGCGGGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACGCCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTa                                                                                                                                                                 <  2:140929/239‑1 (MQ=255)

                                                                                   cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCCGCGGGTTCTTTTGCGCATCGGATATCGGGTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGc                                                                                                                                                               <  1:718800/238‑1 (MQ=255)

                                                                                   cGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGc                                                                                                                                                               <  1:469786/238‑1 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGTGGGGGGCTATCGTTTTCGCAAGATTGCCCGCCCTG‑CC‑GGGCTGGGGCGGGGGGGGGGCAAACGTCGGGAAAAA‑Tcgcggttggcgg                                                                                                                                                             >  2:753080/1‑228 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTATCGGTTACGGAGGGTTGCCGGCCTGG‑GC‑GGGCTGCGGCGGGGGGGGGGCAATCGTCGGGAAATA‑TCAGG‑TTAGcccg                                                                                                                                                            >  1:1150902/1‑237 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCgg                                                                                                                                                            >  1:887086/1‑239 (MQ=255)

                                                                                     tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGAACCCATTTTTGGCGGCAAAGCGGGCAGCTTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATAACGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGGCCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑GCAGG‑TTAGCCgg                                                                                                                                                            <  2:144197/239‑1 (MQ=255)

                                                                                      gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGGCCTGG‑CC‑GGGCTGCGGCGGGGGGGGGACAATCGTCGGGGAATA‑TTCGG‑TTAGCCggg                                                                                                                                                           >  1:1170317/1‑238 (MQ=255)

                                                                                      gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGGTTACGGAAGATTGGCGGCCTGG‑CC‑GGACTGCGGCGTGTGGTTGGCAATCGTCCGGGAATT‑TCAgggttgacggg                                                                                                                                                            >  1:955537/1‑230 (MQ=255)

                                                                                        aGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGAt                                                                                                                                                          <  2:851556/238‑1 (MQ=255)

                                                                                           gTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGtt                                                                                                                                                       <  1:1095284/238‑1 (MQ=255)

                                                                                            tGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTATCCTTTACGAAGGGTTGCCGAACGGG‑CC‑GGGCTGCGGCGGGGGGGGGACACTCGGCGGGAAATA‑TTGGG‑TTGGCCGGGTGGtct                                                                                                                                                     >  2:241300/1‑239 (MQ=255)

                                                                                                    ggggATATGCGGGGGGGCTGGCGCCCTCGAGGGACACCATTTTTGGCGGCAAGGCGGGCGGCGTGTTCTTTGGCGCATCGGATATGGGCGGGGTGGTGGGGCATTCGTATATCGTGTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGcc                                                                                                                                             <  2:132768/236‑1 (MQ=255)

                                                                                                       gttaggCGGTGGCGCTGGCGCCTCCGATGGACACCTTTTTTGGCGGCAAAGCGGGAAGCGTGTTTTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑Ac                                                                                                                                         <  2:476594/233‑1 (MQ=255)

                                                                                                       gattggCGGTGGCGTGGGCGACCTCGATGGACACCATTGTGGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATAGCGGTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGGCCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑Ac                                                                                                                                         <  2:469581/233‑1 (MQ=255)

                                                                                                                 ggCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGGCTGCGGCGTGTGGTGGACAATCGGCGGGGAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTcgcg                                                                                                                             >  1:1369334/1‑239 (MQ=255)

                                                                                                                  gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGAATATCGTTTTAGAAGGAATGCCGGACCGG‑GC‑GGACCGAGGGGGGGTGTGGGAAAACGTGGAGGAAAT‑TCAAG‑GTTGTCGGCGGTTCTCT‑ACGCCG‑ACCGcccattgcac                                                                                                                             >  2:793489/1‑231 (MQ=255)

                                                                                                                  gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGGGAAAAA‑TCAGG‑GTAGCCGGGTGTTCTCA‑GCGCCG‑ACCCCCA‑TTCGCGt                                                                                                                            >  1:1103793/1‑239 (MQ=255)

                                                                                                                  gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGGGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGt                                                                                                                            >  1:553286/1‑239 (MQ=255)

                                                                                                                     cTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGa                                                                                                                                                                                                                        >  1:274649/1‑151 (MQ=255)

                                                                                                                     cTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGa                                                                                                                                                                                                                        <  2:274649/151‑1 (MQ=255)

                                                                                                                                  tGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTccc                                                                                                             <  2:887086/238‑1 (MQ=255)

                                                                                                                                          aTTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGGAAAAATTCCCTACCGCTGa                                                                                                    >  1:29868/1‑239 (MQ=255)

                                                                                                                                            tgttGGCGGAAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGTTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGaaa                                                                                                  <  2:1174181/237‑1 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTCTCGTTTTCGAAGGGTTGCCGACCCGG‑CC‑GGACTGCGGCGTGTGGTGGGCAAACGTCGAGAAATA‑TAAGG‑GTAGCCGGATGTTCTCC‑GCGCCC‑ACCCCCC‑TTTGCGGGCTGAAAAAAATCCCTACCGCTGCAATTCGCac                                                                                           >  2:701620/1‑238 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGaaa                                                                                                                     <  2:73102/213‑1 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGaaa                                                                                                                     >  1:73102/1‑213 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                            <  1:985771/238‑1 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGGGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATTGCGACTCTCGTTTACCCAGGAATGCCGACCCTG‑CC‑GGACTGCGGCGTGTGGGGGGCCATCCGCGAGAGATA‑TCCGG‑TTAGCCGGGTGTTTTCC‑GCGCCG‑GCCCCCC‑TTCCCCGGCCGGAAAAATTCCCTACCGCTGGAATTCTCaa                                                                                           >  2:1084678/1‑239 (MQ=255)

                                                                                                                                                   ggcAAAGCGGGCAGCGTGTTCTTTTGCGCAGCGGATATCGGCTGGGTGGGGGGGCCTTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                            <  1:60135/238‑1 (MQ=255)

                                                                                                                                                                  gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                               <  1:793489/236‑1 (MQ=255)

                                                                                                                                                                  gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                               <  2:448966/236‑1 (MQ=255)

                                                                                                                                                                    gtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTTTCGGTTTCGGAGGATTGCCGGCCTGG‑CC‑GGGCTGCGGGGGGGGGGGGGGAAACGTCCGGGAAAA‑TCAGGTTTAGCCGG‑GGTTTTCA‑GCGCCC‑GCCCCCATTTCCGGGGCGGAAAAAATTTCCACCCCCGGAAATT‑GCACAAACCAtctctccctc                                                                           >  2:137418/1‑234 (MQ=255)

                                                                                                                                                                    gtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGc                                                                          <  1:1028603/239‑1 (MQ=255)

                                                                                                                                                                       cTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTgg                                                                       <  1:664675/239‑1 (MQ=255)

                                                                                                                                                                       cTTTTGCCCATCGGATTTCGGCTGGGGGGGGGGGCATTCGGATTGCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGGGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTgg                                                                       <  1:241300/239‑1 (MQ=255)

                                                                                                                                                                        ttttGCGCAGCGGATATCGGCGGGGTGGTGGGTCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGa                                                                      <  2:115180/239‑1 (MQ=255)

                                                                                                                                                                         tttGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGAGGGCGACTATCGTTTACGAAGGATGGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGaa                                                                     <  1:804600/239‑1 (MQ=255)

                                                                                                                                                                             cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGAATGCGGCGTGTGGGGGACAATCGTCGGGAAATA‑TCAGG‑GTTGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTTCCTACCGCCGAAATTCTCACACACGAACCCTCGTCGCTGGAAGTg                                                                  >  2:348512/1‑238 (MQ=255)

                                                                                                                                                                             cgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGAATATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGGGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGt                                                                   >  1:115180/1‑237 (MQ=255)

                                                                                                                                                                                  cggaattCGGCTGGGTGGTGGGGCGCTCGATTATGGTTTACGCGCCGCTGCTGGGGGGGTTGGTGACTATCGTTTACGAAGGCTTGCCGCCGTGG‑CC‑GGGTTGCGGCGTGTGGTGGACAATCGTCGAGAAAAA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTAt                                                            <  2:955537/233‑1 (MQ=255)

                                                                                                                                                                                              gggTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGGCAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGCAATTCGCCAACACGATCTCTCGTCGCTGGGAGGGCTCTATCTGGCTTgaga                                                 >  2:96249/1‑238 (MQ=255)

                                                                                                                                                                                              gggAGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGAGTATCGTTTAAGAAGGATTGCCGCACCGG‑CC‑GGGCTGCGGGGGGTGGGGGACAATCGGCGGGAAATA‑TCAGG‑GTTGCCGGCTGTTTTTC‑GGGGCG‑ACCGCCA‑CTCCCGTGCTGACAAAATTCCCTACCCACGAAATTTGCAAACACGCACTCTCCTTGCTGGAAGTGCTTCATatggctgtcga                                                 >  2:63301/1‑234 (MQ=255)

                                                                                                                                                                                               ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGCAGGATTGCCGGCCTCG‑CC‑GGACCGCGGCGGGGGGGGGGCAAACCTCGGGGAATACTCGGG‑TT‑GCCGGATTGTTTCA‑GCGCCGAACCGCCT‑TCCGCG‑GCTTGAAAAATTCCCTACCCGCGAAATTGGCCAACCCGATCTCCGCTCGCGTGAAGGGCTCTCTtcggctggggca                                                >  2:122710/1‑234 (MQ=255)

                                                                                                                                                                                               ggtgggGGGGCAGTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATct                                                          <  1:545029/228‑1 (MQ=255)

                                                                                                                                                                                               gggggtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACCGCGGGGGGTGGGGGGCAATCGTCGAGGAAAA‑TCAGG‑GTAGACGGGTGTTTTCA‑GCGCCG‑ACCGCCC‑TTAGCGTGCTGGAAAAAATCCCTACCGCTGAAATTCGCAAACACGAACTCTCGTCGCTGGAGGAGGCCTATctggcgtgggaa                                                >  2:517477/4‑231 (MQ=255)

                                                                                                                                                                                                   gtggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCCAAACCGATCTCTCCTCGCTGGAAGTGCCCTATCTGGCTGGAGAAcc                                              >  1:358995/1‑236 (MQ=255)

                                                                                                                                                                                                         cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGGCAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAAAACGATCTCTCGGCGCTGGCAGTGCTCTATCTGGCTGGGGAAACGCTGGaggg                                     >  2:1169728/1‑236 (MQ=255)

                                                                                                                                                                                                         cATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCC‑GCGCCG‑ACCGCCC‑TTCGCGTGCTGGAAAAATTCCCTCCCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGCAGTGCTCTTTCTGGCTTGCGAAcctttctcttt                                     >  2:545029/1‑230 (MQ=255)

                                                                                                                                                                                                                atgtCGTTTAGGGGCGGTTGCTGGGGGGGGTGGCGATTATCTTTTACGAGGGTTTCCGGGCTGGG‑CG‑GGCCTGCGGGGGGTGGGGGACAATCGTCGAAAAATA‑TCAGG‑TTAGCCGGATG‑TCTCAGGCCCGG‑CCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAc                               <  2:1043650/235‑1 (MQ=255)

                                                                                                                                                                                                                 tatCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTTCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAcc                              >  1:774453/1‑238 (MQ=255)

                                                                                                                                                                                                                  atCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTCTCGTTTTAGAAGGATTTCCGACCTGG‑CC‑GGACTGCGGCGTGGGGGGGACAATAGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGGTCTCA‑GCGCCG‑GCCGCCA‑TTCGCGGGCTGAAAAAATTCCCTACCCCTGGAATTCCCCAACACCATCTCTCGTCTGTGGAAGTGGCCCATCTGGGCGGTGAAACGCTGGGCGAGCCGcccc                             >  2:173509/1‑237 (MQ=255)

                                                                                                                                                                                                                   tCGTTTAGGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGCCCTGG‑CG‑GGACGGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGcc                           <  1:753080/239‑1 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGGGGGCGGCTATCGTTTACGAAGGATTGCCGACCCGG‑CC‑GGACTGCGGCGTGGGGGGGAAAATCGTCGAGAAATA‑TCAGG‑GTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGGGCTGAAAAAATTCCCTACCGCTGAAATTCCCAAACAACATCTCTCGTCGCTGGAAGTGCTCTCTCTGGCTGGGGAACCGCTGGACGCGCCCACCCCCCGt                        >  2:1256575/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTTGCTGGAGAACCGCTGGACGAGCCGACCGCCAGt                        >  1:327712/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCC‑TTCCCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGGTCTATCTGGCTTGAGGAACGCTGGACGAGCCGACCGCCAGt                        >  2:1407156/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAAACGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑AACGCCA‑TTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGGCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCCGGAGGAGCCGACCCCCCGt                        >  1:1085762/1‑239 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGGGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGGTGTTCTCA‑GGGCCG‑ACCGCCA‑TTCGCGTGCTGGAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGGCTCTATCTGGCTGGGGAACCGCTGGAAGAGCCGACCGCCa                          >  2:1323198/1‑237 (MQ=255)

                                                                                                                                                                                                                      tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGAATGCGGCGGGTGGTGGGCAATCGTCGAAAAAAA‑TCAAG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCC‑TTCGCGTGCTGAAAAAATTCCCTACAGGTGAAAATTGCCAAAACGATCTCCCGTCCCTGGAAGGGCTCTATCTGGCTGGGGGACCGCGGGACGAGCCGACCGCCacg                        >  2:1011485/1‑237 (MQ=255)

                                                                                                                                                                                                                      ttgccgcgCCGCTGCTGGGGGGGGTGGCGACTATCGTTTACGAAGGATGGCCGCCCGGG‑CC‑GGACGGCGGCGGGGGGTGGACAACCGTCGAAAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGt                        <  2:1085762/235‑1 (MQ=255)

                                                                                                                                                                                                                        tACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGGAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGAGCTCTATCTGGCTGGAGGACCGCTGGACGAGCCGACCGCCAGTTg                      >  2:1272689/1‑239 (MQ=255)

                                                                                                                                                                                                                         aCGCGCCGCTGGTGGGGGGGATGGCGACTATCGTTTACGAGGGATTGCCGCCCTGG‑CC‑GGACTGGGGCGTGGGGGGGAAAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                     <  2:327712/239‑1 (MQ=255)

                                                                                                                                                                                                                         aCGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCC‑GCGCCG‑AACGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGGAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGCCCCCCAGTTgg                     >  1:492781/1‑239 (MQ=255)

                                                                                                                                                                                                                         aCGCGCCGCTGCGGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg                     <  1:96249/239‑1 (MQ=255)

                                                                                                                                                                                                                         aCGCCCCGCTGGTGGTGGGGATGGCGACTAGCGTGACCGAGGGATTG‑CGTCCCGG‑CCTGGAGTGGGGCGGGGGGTGCACACTCGGGGAAAAATT‑TCGGG‑AAAGCATGCGGTCCGAT‑GCCCCG‑ACCGCCA‑TTGGCGGCCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCGCTCGTCGCTGGAAGTGCTCTATCGGGCTGGATATCCGCTGGACGAGCCGCCCGCCAGTTgg                     <  2:1097892/239‑1 (MQ=255)

                                                                                                                                                                                                                           gcgcCGCTGCTGGGGGGGATGGCGATTATCGTTTAGGAAGGATTGCCGACCGGC‑CC‑GGACGGCGGCGTGTGGTGGACAATCGTCAAGAAATA‑TAAGG‑TTAGCCGGATGTCCTAA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                   <  2:38526/239‑1 (MQ=255)

                                                                                                                                                                                                                           gcgcCGCCGCTGGCGGGTGGGGCGTCTACCGTTTACGAGGGTGTGTCGACCTGG‑CG‑GGCCTGCGGGGTGTGGGGAACAATCTTCGAGAAATA‑CCAGG‑TTAGCGGGATGTCCCCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGAAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                   <  2:135206/239‑1 (MQ=255)

                                                                                                                                                                                                                           gccccgcGGGTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGGGGTGGACAATCGTCGAGAAATA‑CCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGt                   <  1:1084678/236‑1 (MQ=255)

                                                                                                                                                                                                                              ccgcTGCTGGCGGGGATGGCGACTATCGTTTTCGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGGGTGTGGTGGGCAATCGGCGAGGAATA‑TCAGG‑GTAGCCGGATGTTCTCA‑GCGCCC‑GCCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACCCGATCTCCCCGCCCTGGAAATGCCGTATCTGGCTGGGGCACCGCCGGACGAGCCGCACGCCCCTTGGGTggg                >  2:489961/1‑237 (MQ=255)

                                                                                                                                                                                                                               cgcTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGGGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑GTAGCCGGGTGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCCATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGc               >  1:1257606/1‑239 (MQ=255)

                                                                                                                                                                                                                               cgcTGCTGGCGGGGAGGGCGACTATCGTTTACGAAGGATTGCCGGCCCGG‑CC‑GGACCGCGGCGGGTGGTGGACAATCCTCGAGAAATA‑TCAGG‑TTTGCCGGGTCTTCTCA‑GCCCCC‑ACCGCCC‑TTCGCGTGCTGAAAAAATTCCCTACCCCTGAAATTCCCAAACACCAACCCTCGTCGCTGGTAGTGCCACATCCTGGTGGGGCACCGCTGGAAGAGCCGACCGccccatgggcgag                >  2:309226/1‑227 (MQ=255)

                                                                                                                                                                                                                                      gggggggTTGCCGACTGTCGTTAACGAAGGATGGCCGACCTGG‑CC‑GGGCTGGGGCGGGTGGTGGAGAATCGTGGAAAAAGT‑CCAGT‑TTAGCCGGATGTTCCCG‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACg         <  2:898179/235‑1 (MQ=255)

                                                                                                                                                                                                                                            gATGGCGACTATCGTTTTCGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGGGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCGGGAAAAGCTCTATCTGCCTGGGGAACCGCTGGACGAGCCCCCCCCCCAGTGGGTGCGCAATACGCTGGAtg  >  2:865327/1‑239 (MQ=255)

GCGCGGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGG‑CC‑GGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATA‑TCAGG‑TTAGCCGGATGTTCTCA‑GCGCCG‑ACCGCCA‑TTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATACGCTGGATG  >  NC_000913/353369‑353843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`