BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,027,146	C→G	13.7%	A207G (GCG→GGG)	fre →	NAD(P)H‑flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,027,146	0	C	G	13.7%	257.9 / 4.0	101	A207G (GCG→GGG)	fre	NAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand): ref base C (45/38); new base G (9/6); total (54/47)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GTAACCCAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATA  >  NC_000913/4026909‑4027383

gTAACCCAAACCGTGATATCTCCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGc                                                                                                                                                                                                                                                     <  2:961576/238‑1 (MQ=255)

        aaCCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTTCAGGGTCACGGGACGCT‑GGGCGGGCATG‑ATATTTTTATTTCCGGGCGGTTTTGGATGGG‑G‑GAAA‑t‑tt                                                                                                                                                                                                                                         >  1:338390/1‑237 (MQ=255)

        aaCCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGc                                                                                                                                                                                                                                        <  1:1219847/239‑1 (MQ=255)

             tGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTTGCGGAGCACGGGACGCT‑GGCAGAGCATG‑ATATTCATATTTCCGGGCGTTTTTTGGTGGCGG‑AAAA‑T‑TGccccc                                                                                                                                                                                                                                    >  2:1219847/1‑237 (MQ=255)

              gATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTTCAGGGTCACGGGACGCT‑GGCAGAGCATG‑ATATCTCTATTTCCCGACGTTTCGTGATGGG‑G‑GAAA‑T‑TTCCcgc                                                                                                                                                                                                                                    >  1:420121/1‑237 (MQ=255)

                tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGAt                                                                                                                                                                                                                                 <  2:6170/238‑1 (MQ=255)

                tatCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCGGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGAt                                                                                                                                                                                                                                 <  1:1401239/238‑1 (MQ=255)

                tagcACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGAt                                                                                                                                                                                                                                 <  2:817789/235‑1 (MQ=255)

                    aCCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGGTCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGGCGGTTTTGGATGGG‑G‑AAAA‑T‑TGTCCGCGATCT‑Gtt                                                                                                                                                                                                                           >  1:920556/1‑239 (MQ=255)

                        tttACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGGTCACGGTGCGCC‑GGGAGAGCACG‑ATATCTATATTGTCGGGCGGTTTGTGTTGGG‑G‑GCAA‑A‑TGCCCGCGAGCT‑Gttttt                                                                                                                                                                                                                        >  2:356853/1‑237 (MQ=255)

                          tACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAg                                                                                                                                                                                                                     <  1:157352/239‑1 (MQ=255)

                           aCTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGt                                                                                                                                                                                                                    <  1:126031/239‑1 (MQ=255)

                                ggcgggcgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGAATTGCCGGGTTACGGGACGCT‑GGCAGAGCCTG‑GTATCTATATTGCCGGGCGGTTTTTGGAGGC‑G‑GAAA‑A‑TGCCCGCGATCT‑GTTTTGCAGGGAGCg                                                                                                                                                                                                               >  1:710311/1‑239 (MQ=255)

                                    ggcgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGGAGGGTCCCGGGACGCC‑GGCAGAGCATG‑AGATCTATCTTTGCGGGCGTTTTTGGATGGC‑G‑GAAA‑T‑TGCCCGCGGTTTGGTTTTGcgtgggaggaat                                                                                                                                                                                                            >  1:1300486/1‑228 (MQ=255)

                                      cgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATg                                                                                                                                                                                                          <  1:799485/238‑1 (MQ=255)

                                      cgTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGGATTGCACGGTCCCCGGAGGGT‑GGCCGAGCATG‑GTAGCTCTAATGTCGGGCGGTTTTTGAGGGG‑G‑GAAA‑A‑TGGCCCCTATCT‑TTTTTGTCGTGCGCGTacagc                                                                                                                                                                                                         >  2:1245417/1‑235 (MQ=255)

                                         gAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGcgcg                                                                                                                                                                                                      <  2:384302/239‑1 (MQ=255)

                                              gcaggaTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCGTCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGa                                                                                                                                                                                                   <  1:717934/237‑1 (MQ=255)

                                               cagcaTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAg                                                                                                                                                                                                 <  2:1194279/238‑1 (MQ=255)

                                                   aTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATc                                                                                                                                                                                              <  2:188327/237‑1 (MQ=255)

                                                    tCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCAGCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGc                                                                                                                                                                                            <  1:629705/238‑1 (MQ=255)

                                                     cTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGc                                                                                                                                                                                                                                        <  1:122561/194‑1 (MQ=255)

                                                           gATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTgg                                                                                                                                                                                    <  1:956585/239‑1 (MQ=255)

                                                            aTCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGAATTGCAGGAGGAAGGTAGGCT‑CGGAGAGCATG‑ATATTTAAATTGGCGGACGGTTTGGGATAGG‑G‑AAAATT‑TGCCCG‑GGTCT‑GTTTTTTTGTCAGGTTAAGTGGGGGGGAGAACGCCTGTTTgg                                                                                                                                                                                    >  2:1112472/1‑238 (MQ=255)

                                                               tctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGAACACGGGACGCC‑GGCAGAGCATG‑ATATCTATATTTCCGGGCGGTTTTTGATGGC‑G‑AAAA‑T‑TGCCCCCGATCT‑GTTTTTTAGTGAGCGTAATGTGCGGGGAGATCACCCGTTTgggggt                                                                                                                                                                                >  1:421370/1‑235 (MQ=255)

                                                                  gCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGGAGGGTCACGGGACGCC‑GGGCGAGGAAG‑ATATCTAAATTGCCGGGCGGTTTGGGGTGGC‑G‑GAAA‑A‑TGCCCGCGATCT‑GGTTTTCAGTGAGGGGAAATGGCGGGGAGATCGCCTGTTTgggggttgg                                                                                                                                                                             >  2:332361/1‑232 (MQ=255)

                                                                    gAGCTTGAGGCGCTTTCGTTGAATCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGt                                                                                                                                                                            <  1:1020915/238‑1 (MQ=255)

                                                                    gAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGt                                                                                                                                                                            <  1:587079/238‑1 (MQ=255)

                                                                        ttGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGGTCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTTCCGGGCGGTTTTAGATGGC‑G‑GAAA‑T‑TGCCCGCGATCT‑GTTTTTCCGTGGGCGTAAAGCGCGGGGAGATCGCCCGTTTGGCGGTGCGTTTGCa                                                                                                                                                                       >  1:1084838/1‑239 (MQ=255)

                                                                        ttGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGc                                                                                                                                                                        <  1:1339223/238‑1 (MQ=255)

                                                                        ttGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCa                                                                                                                                                                       <  1:503377/239‑1 (MQ=255)

                                                                        ttGAGGCGCGTTCGTGGAAGCGTCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCa                                                                                                                                                                       <  2:1160900/239‑1 (MQ=255)

                                                                           aGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGGGTACGGGGCGCT‑GGCAGGGCGTG‑GTATGTATATTTGCGGACGGTTTTTGATGGC‑G‑AGAA‑A‑TGCCCCCGGTCC‑TTTTTTCCGTGGGGCGAATGGCCGGGGAGAGCGCCTGTTTTGCGGTGCGGTTTCAttt                                                                                                                                                                    >  2:839497/1‑239 (MQ=255)

                                                                           aGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCCGAGCATG‑ATATCTATATTTCCGGACGGTTTGTGATGGC‑G‑AAAA‑T‑TTCCCGCGCTCT‑TTTTTTCAGGGAGCGGAATGCGCGGGGAGATCGCCCGGTTGGCGATGCGGTTTCCttt                                                                                                                                                                    >  1:147870/1‑239 (MQ=255)

                                                                                  ttCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGGAGGATCACGGGACGCT‑GGCCGAGCATG‑ATATCTATATTTCCGGGCGGTTTTAGAAGGC‑G‑AAAA‑T‑TGCCCGCGAGCT‑GTTTTGGAGTGAGCGGAATTCGGGGGGAGATCCCCTGGTTTGGGGTGCGTTTGCATTTAttttgg                                                                                                                                                             >  1:346478/1‑234 (MQ=255)

                                                                                              tCCTGTGCTGCAGGGGGGGCCGTTGGTTGAACAACCGGAAGGGGGCTGGCGTGGGCG‑TCCTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGGT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGAGGGC‑G‑AAAA‑T‑TGCCCGCTATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCTTTTATCTGAGATATAAAAAAAc                                                                                                                                                 <  2:719900/239‑1 (MQ=255)

                                                                                              tCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATg                                                                                                                                                                               <  1:484122/209‑1 (MQ=255)

                                                                                                        cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGAAATGCAGGGTCCAGGGAAGCC‑GGGAGAGCATGAATATC‑ATTTTTTCGGGCGGTTTGTGATGGG‑G‑GAAA‑T‑TGGCCCCGCTCT‑GTTTTTTAGTGAGGGGGATGGGGGGGGAAATCGCCTGGTTGGGGGGGCGGTTGCttt                                                                                                                                                                     >  2:484122/1‑209 (MQ=255)

                                                                                                                   gggggtTGAACAAGCGGAGGCGGGC‑GGCGGGGGCGATAGTGG‑GGCGTGTTGAAG‑GGGGGTATTGCAGGATGAGGTGACGTT‑GGCAGAGCATG‑ATATCTATATGGCGGAACGTTTGGAGAGGGC‑A‑AAAG‑T‑TGCCCGCTATCT‑GTTTGCCGGGGAGGGTAATGCGCGGGAAGACCCCCTGTTGGGCGAGGGGTTTGCATTTATCTGAGATAGAAAAAAACCCGCCCCTGACAGGCGG‑gaa                                                                                                                            <  2:866824/235‑1 (MQ=255)

                                                                                                                              aacGGGAGGGGGGCTGGCGGGGGGG‑TACGGGCACGGTTTT‑ACC‑GGCGGTTTTGCAGGATCACGGTACGTT‑GGCAGAGCATG‑ATATCTATATTGCCGGACTTTTTGAGAGGGC‑G‑AAAA‑T‑TGCCCGCGTTCT‑GTTTTGCGGTGAGCTTAATGCGCGGGAAGATCGCCTGTTTGGGGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTa                                                                                                                <  2:1122400/239‑1 (MQ=255)

                                                                                                                                 cgagggggggcgggcGCGGGCG‑GACTGGCA‑CGCGTT‑AACGGGCGGTATTGAGGTATC‑CGGTTCGGTGGGAAGTCAATG‑ATATCT‑TATTGGCGGGCGGTTTGAAGGGGC‑GAAAAA‑T‑TGCCGGGGTTTT‑TTTTTGCAGGGACGTTAAGGCGCGGAAAGATCGCTTGTTTGCGGATGCGTTTGAATTTATCTGAGATATAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTaaa                                                                                                              <  2:79290/231‑1 (MQ=255)

                                                                                                                                    gggggggCGGGGGTGGGCT‑TACGGGCCCCGGTTT‑AAG‑GGCGGTTTTGCAGGATCACGGTACGGT‑GGCAGAGCATG‑ATATCTATATGGCCGGACGTTTTGAGAGGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCGGTGAGCGTAATGGGGGGGAAGATCGCTTGTTTGGCGATGCGTTTGAATTTATCTGAGATATAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTg                                                                                                           <  2:421319/234‑1 (MQ=255)

                                                                                                                                         ggtgggggTGGGCG‑TCCGGGCACCGTGTT‑AAC‑GGCGTTATTGCAGGATCACGGTACGCG‑GGAAGAGCATG‑ATATCTATTTTGCCGGCCGTTTGGAGAGGGC‑G‑AAAT‑T‑GCCCCGCGTTTT‑TTTTTGCAGGGAGCGTAATGCGCGGGAAGATCGCCTTTTTGGCGATGCGTTGGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTAtt                                                                                                      <  2:1206996/231‑1 (MQ=255)

                                                                                                                                          gCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGAACACGGTACGCT‑GGCAGGGCATG‑ATATCTATATTGCCGGGGGGTTTGTGGTTGC‑G‑AAAA‑A‑TGCCCCCGGTCT‑GTTTTGCAGTGAGCGGAATTACGGGGGAGGTCGCCTGCTTGGCGGTGCGTTTTCTTTTTTCTTAGATCATAAAAAACCCGCCCCGCACAGGCGGCGAGGAACCGCACCCaaaagctattct                                                                                                     >  2:721806/1‑230 (MQ=255)

                                                                                                                                             ggggTGGGCG‑TATGGGCACCGTGTT‑AAG‑GGGGGTATTGCAGGATCAGGGTACGTG‑GGCAGAGAATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑GGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGt                                                                                                  <  2:920556/235‑1 (MQ=255)

                                                                                                                                              gCGGGGGCG‑TCCGGGCACCGTTTT‑AAC‑GGGGGTATTGCAGGATCGCGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGGGATCT‑GTTTGGCAGTGGGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTg                                                                                                 <  2:1084838/238‑1 (MQ=255)

                                                                                                                                                   ggCG‑TACGGGCCCGGTGTT‑ACG‑GGTGGTTTTGCAGGATCACGGTACGCG‑GGCAGGGCATG‑ATATTTATTTTGCCGGACTTTTTGAGATGGG‑G‑AAAT‑T‑TGCCCGCGATCT‑TTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCAtt                                                                                            <  2:706836/238‑1 (MQ=255)

                                                                                                                                                     cG‑TACTGGCACCGTGTT‑AAC‑GGGGGTATTGCAGGATCAGGGTACGCT‑GGCAGAGCATT‑ATATTTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGGGATCT‑GTTTTGCAGTGGGGGTAATGCGGGGAAAGACCGCTTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTa                                                                                          <  2:1300486/238‑1 (MQ=255)

                                                                                                                                                     cG‑TACGGGCACCGTGTT‑ACC‑GGGGGTATTGCAGGATCGGGGTACGCT‑GCAAGAGCATG‑TTATCTATATTGCCGGACGTTTTGAGAGGGC‑A‑AAAA‑T‑TCCCCGCATTCT‑GTTTTGCAGTGAGCGTAAGGCGCGGGAAGACCGCCTGTTTGGCGATGCGTTTGCTTTTATCTGAAATATAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTa                                                                                          <  1:1062687/238‑1 (MQ=255)

                                                                                                                                                         aCTGGCACCTTGTT‑ACC‑GGCGGTATTGAAGGATCACGGTCCGCT‑GGCGGAGAATG‑ATATCTATATTGCCGGACTTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑TTTTGGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATAAAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAAc                                                                                                                        <  2:191916/205‑1 (MQ=255)

                                                                                                                                                               aCCGTGTT‑AAC‑GGCGGGATTGGCGGGTCACGGTACGCT‑GGCCGAGAATG‑ATATCTATATTTTCGGGCGTTTTTGGATGGG‑G‑AAAA‑A‑TTCCCCCCGTTT‑GTTTTTCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATTCGTTTTCATTTTTCTGTGATATTAAAAAAACCGCCCCTGCCAGGCGG‑GGAGAAAGGCCACTAAAATGTTATTTACTGGCATTTTTATCTATgtc                                                                                >  2:1197685/1‑237 (MQ=255)

                                                                                                                                                                 cGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGAt                                                                                                                                                                                                                                 <  1:1238752/94‑1 (MQ=255)

                                                                                                                                                                    gtT‑AAC‑GGTGGTATTGGAGGTTCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGAGGGG‑A‑AAAA‑T‑TGCCCGCGTTCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTAt                                                                            <  2:338390/238‑1 (MQ=255)

                                                                                                                                                                         aC‑GGCGGTATTGCAGGACCCCGGTACGCG‑GGCAGAGATTG‑ATATCTTTTTTGCCGGACGTTTTGAGATGGA‑G‑AAAA‑T‑TGCCCGCGTTCT‑GTTTTGCAGTGAGCGTAATGCGGGGGAAGATCGCTTGTTTGGCGATGCGTTTGCATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGc                                                                       <  2:904037/239‑1 (MQ=255)

                                                                                                                                                                                           tCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGCTATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAAATGTTATTCAGTGGCATTTAGTACTATGACGGATTTGGCAAAACATGCCCCATGc                                                       >  1:1417508/1‑238 (MQ=255)

                                                                                                                                                                                           tCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCg                                                      >  2:264849/1‑239 (MQ=255)

                                                                                                                                                                                                   cGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTc                                              >  1:1300089/1‑239 (MQ=255)

                                                                                                                                                                                                   cGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGAACTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTc                                              >  1:278643/1‑239 (MQ=255)

                                                                                                                                                                                                   cGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGGGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTc                                              >  2:1412031/1‑239 (MQ=255)

                                                                                                                                                                                                   cGCT‑GGAAGAGAATA‑TTATCTTTTTGGCCGGAGGTTTGGAGAGGGC‑G‑AAAA‑T‑TGCCCGGGTTTT‑TTTTTGCAGGGAGCGTAAGGCGGGGGAAGACCGCTTGTTGGGCGATGCGTTTGCATTTATCTGAAATAAAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTc                                              <  2:147870/239‑1 (MQ=255)

                                                                                                                                                                                                          cAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GGAGAACGGCAACTAAACTGTTGTTCAGGGGCCTTTAGATCTATGGCGTATCTGGCAAAACATTCCCGATCCGACGCTGGcgcgctt                                        >  2:1003812/1‑236 (MQ=255)

                                                                                                                                                                                                          cAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCTACGAAACTGTTATTCAGGGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCt                                        >  2:903545/1‑239 (MQ=255)

                                                                                                                                                                                                          cAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATTCGCCGCttgccggtct                                        >  2:194363/1‑230 (MQ=255)

                                                                                                                                                                                                          cAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCt                                        >  1:1265155/1‑239 (MQ=255)

                                                                                                                                                                                                            gagCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGGGGCATTTAGATCTATGACGTATCTGGCCAACCCTGCCCGATTCGACGCTGTCGCGTCTTa                                      >  2:688626/1‑239 (MQ=255)

                                                                                                                                                                                                               cttG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑A‑AAAT‑T‑TGCCCGCGATTT‑GTTTTGCAGGGAGCGTAATGCGCGGGAAGATCGCCTTTTTGGCGATGCTTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCg                                                                                                                                 <  2:1409819/144‑1 (MQ=255)

                                                                                                                                                                                                                aTG‑ATATTTATATTGCCGGTCGTTTGGAAATGGC‑G‑AAAA‑T‑TGCCCGCGATTT‑GTTTTGCAGTGGGCTTACTGCGCGGGAAGATCCCCTGTTGGGCGATGCGTTGGCTTTTATCTGAAATATAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGt                                  <  2:465167/239‑1 (MQ=255)

                                                                                                                                                                                                                aTG‑ATATATATATGCCCGGACGTTTTGAGAGGGA‑G‑AAAA‑T‑TGCCCGAGATCT‑GTTGGACGGTGAGCGTAATGCGCGGGAAAACGTCCTGTTTGGGGAGGCTTTTCAATTTACCGGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGGCGCGTCTTATCg                                   <  2:924070/238‑1 (MQ=255)

                                                                                                                                                                                                                 tG‑ATATTTTGATTGCCGGGCGTTTTGGGAGGGG‑G‑AAAA‑T‑GGCCCGCGATCT‑GTGTTGCAGTGAGCGTAAGGCGCGGGAAGATCGCCTGTTTGGCGATGAGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTg                                 <  1:206741/239‑1 (MQ=255)

                                                                                                                                                                                                                    atttttataTTGCGGGAGGTTGGGAGGTGCG‑G‑AAAG‑T‑TGCCGGGGATCT‑GTTTTGCGGGGAGTGTAAGGCGGGGGAAGATCGCCTGTTGGGCGATGGGTTGGAATTTATCGGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGcc                               <  2:420121/234‑1 (MQ=255)

                                                                                                                                                                                                                     agtCTATTTTGCGGGACTTTTTGAGAGGGG‑G‑AAAA‑G‑TCCCCGGAGTTT‑TTTTTGCGGTGAGGTTAATGGGGGGGAAGAGCGCCTGTTTGGCGTTGCGTTTGCATTTATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGcc                               <  2:772197/236‑1 (MQ=255)

                                                                                                                                                                                                                        cTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGCTGCGACGCTGTCGCGTCTTATCGTCCCTACa                           >  1:481476/1‑239 (MQ=255)

                                                                                                                                                                                                                          atatTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACgg                                                                                                                      >  1:1409819/1‑146 (MQ=255)

                                                                                                                                                                                                                             ttGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGGGGCATTTAGATCTATGAAGTATTTTGAAAACCATGCACGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATaa                      >  1:1058680/1‑238 (MQ=255)

                                                                                                                                                                                                                                ccGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGGCACTAAAATGTTATTTAGTGGCATTTAGATCCATGACGTATCCGGCAAACCATTCCCGATGCGACGCTGTCCCGTCTTATCGGGCCTACAAATAGTc                    >  1:1230443/1‑238 (MQ=255)

                                                                                                                                                                                                                                ccGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTTCCTACAAAAAGTcc                   >  1:1217792/1‑239 (MQ=255)

                                                                                                                                                                                                                                ccGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc                   >  1:391336/1‑239 (MQ=255)

                                                                                                                                                                                                                                ccGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTcc                   >  1:761758/1‑239 (MQ=255)

                                                                                                                                                                                                                                ccGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGGATCTGGCAAACCATGCCCGATGCGACGCTGTCGCCTCTTTACGTTCCTACAAATAGTc                    >  2:1400807/1‑238 (MQ=255)

                                                                                                                                                                                                                                ccGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAAATGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGCCGCCTCTTATTGTGCCTACAAATAATc                    >  2:909785/1‑238 (MQ=255)

                                                                                                                                                                                                                                  ggACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGTCTACAAATAGTCCGa                 >  2:782654/1‑239 (MQ=255)

                                                                                                                                                                                                                                   gACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCCACTAAACTTTTATTCAGTGGCATTTAGAACTATGACGTATCTTGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCTTCCCTACCAACAGTCCGa                 >  2:389864/1‑238 (MQ=255)

                                                                                                                                                                                                                                   gACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTTTTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCCCGTCTTTTCTTGCCTACaaaacatccga                 >  2:89630/1‑230 (MQ=255)

                                                                                                                                                                                                                                   gACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGCCGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGa                 >  1:497191/1‑238 (MQ=255)

                                                                                                                                                                                                                                   gACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACCAATAga                     >  1:772197/1‑233 (MQ=255)

                                                                                                                                                                                                                                   gACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGGATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGa                 >  1:504882/1‑238 (MQ=255)

                                                                                                                                                                                                                                     cGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCCACTAAACTGTTATTTAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTAGCGTCTTCACGCTCCTACAAATAAGCCGAAc               >  1:1250660/1‑238 (MQ=255)

                                                                                                                                                                                                                                     cGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAAcc              >  1:93254/1‑239 (MQ=255)

                                                                                                                                                                                                                                      gTTTTGAGGGGGG‑A‑AAAA‑T‑GGCCCGCGATTT‑TTTTGGCAGTGAGCGTAAGGCGCGGGAAGATCGCTTGTTTGGGGATGGGTTTGCTTTTATCTGAGATAAAAAAAACCCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCg                                   <  2:977476/217‑1 (MQ=255)

                                                                                                                                                                                                                                          tGAGATGGC‑A‑AAAA‑T‑TGCCGCGGTTCT‑GTTTGGCAGGGAGGGTAAGGCGGGGGAAGATCGCCTTTTTGGCGATGCGTTGGAATTTATCTGAAATAAAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAgg         <  1:839497/239‑1 (MQ=38)

                                                                                                                                                                                                                                           gagaTGG‑‑G‑AAAA‑TGTCCCCGGGTTTT‑GTTTTGAAGTGAGGGTAAGGGGCGGGAAGACCGCGTGTTGGCCGATGCGTTTGCATTAATCTGAGATAAAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGc        <  2:1168785/239‑1 (MQ=38)

                                                                                                                                                                                                                                              aTGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTCGATCTATTCCGTATCTGGCAAACCATTCCCGCTGCGACGCTGTCGCGTCTTTTCGTGCCTAAAAACAGTCCGAACCGTCAGCCgg     >  2:628042/1‑239 (MQ=255)

                                                                                                                                                                                                                                                ggC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGGGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTaaa                                                                                                              <  2:756727/132‑1 (MQ=255)

                                                                                                                                                                                                                                                ggC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTaaa                                                                                                              >  1:756727/1‑132 (MQ=255)

                                                                                                                                                                                                                                                ggC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACaaa                         >  1:977476/1‑217 (MQ=255)

                                                                                                                                                                                                                                                ggC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGAt   >  1:767881/1‑239 (MQ=255)

                                                                                                                                                                                                                                                ggC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGAt   >  1:827526/1‑239 (MQ=255)

                                                                                                                                                                                                                                                ggC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAAATAACCTGGTATTCa                                                                                                    >  1:756713/1‑142 (MQ=255)

                                                                                                                                                                                                                                                 gC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GCAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTTGCAAACCATGCACGCTGCGACGCTGTCGCGTCTTATCGTGCCTACAACTAGTCCGAACCGTAGGCCgggtt  >  1:1212208/1‑236 (MQ=255)

                                                                                                                                                                                                                                                 gC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCCCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGTCCGCATa  >  2:437795/1‑239 (MQ=255)

GTAACCCAAACCGTGATATCACCATTTACTGGGGCGGGCGTGAAGAGCAGCATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCG‑TACTGGCACCGTGTT‑AAC‑GGCGGTATTGCAGGATCACGGTACGCT‑GGCAGAGCATG‑ATATCTATATTGCCGGACGTTTTGAGATGGC‑G‑AAAA‑T‑TGCCCGCGATCT‑GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGG‑GAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATA  >  NC_000913/4026909‑4027383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`