New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4345644 =	155 (1.240)	3 (0.030)	3/398	NT	2.1%	intergenic (‑86/+36)	yjdF/fumB	DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
	?	NC_000913	4345664 =	134 (1.180)					intergenic (‑106/+16)	yjdF/fumB	DUF2238 family inner membrane protein/anaerobic class I fumarate hydratase (fumarase B)
Rejected: Frequency below/above cutoff threshold.

GGCGGCTTCTACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4345895‑4345644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctggcagcatgctgccaggCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGC  >  NC_000913/4345664‑4345894                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     GGCGGCTTCTACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGGATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCCACAAACAGTGCGCGAACTGCACTAAGTAACCCCTTCGGGCCAGCGCCTGGCc                                                                                                                                                                                                                                                      >  2:1289345/1‑238    GGCTTCTAACTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTGCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGC                                                                                                                                                                                                                                                    <  2:131442/238‑1       TTCTACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATG                                                                                                                                                                                                                                                 <  2:1350286/238‑1       TTCTACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATG                                                                                                                                                                                                                                                 <  2:461791/238‑1        TCTACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCAT                                                                                                                                                                                                                                                  >  1:550154/1‑236                                                 CAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGC                                                                                                                                                                                                       <  2:367837/238‑1                                                                                    TCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGT                                                                                                                                                                    <  1:159527/238‑1                                                                                                                                                          GACAAAGGTAACTGATTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACC                                                                                              <  2:159527/238‑1                                                                                                                                                                                                                                            GGCAGCATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGT           >  2:893530/1‑239                                                                                                                                                                                                                                                 CATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCG                                <  1:1044899/213‑1                                                                                                                                                                                                                                                 CATGCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCG                                >  2:1044899/1‑213                                                                                                                                                                                                                                                    GCTGCCAGGCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGCTTTTCTAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCGGCCCGCCGATGCTACAGAGGTAGAAACC    >  2:102562/1‑238                                                                                                                                                                                                                                                      gacagGGCGCTGGGCCGAACAGGTTACTTGGTGCATTGCGCTCACTGTTTGTTGACGATTTGGTGGAAAAAGTCGTTACCTTGGTCACCGACCAGGATAAACGCGGGGAAAGCTTATACTGCGATTTTCCAGATGGCTTCCATACCCAGCTGCGGATAAGGGACGCTCTCCAGATGCTGGAGGCTCTGCTGCGCCAGTCCCGCCGGGGGACCGCCGATGCTACCGAGGTAGAAGCCGC  <  2:904693/233‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     GGCGGCTTCTACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATCTGGAGTGCGTCGCTTATCCGGAGCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACTTCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAACCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4345895‑4345644 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cctggcagcatgctgccaggCGCTGGGCCGAAGAGGTTACTTAGTGCAGTTCGCGCACTGTTTGTTGACGATTTGCTGGAAGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGC  >  NC_000913/4345664‑4345894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.