Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,518	C→T	14.7%	A19V (GCT→GTT)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,518	0	C	T	14.7%	199.2 / 7.2	100	A19V (GCT→GTT)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (32/48);  new base T (2/12);  total (37/63)
Fisher's exact test for biased strand distribution p-value = 7.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATA  >  NC_000913/3206281‑3206750                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCt                                                                                                                                                                                                                                           <  2:237923/239‑1 (MQ=255)        tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGATTTTTTCAGACAAAAAAAAAAA‑ATGG‑A‑GTTG‑GC‑ACG‑AT‑GAGCGGGAGGAATAAGTAACAAGGAGAG‑AAAAAATTGGCAGAG‑ATTTTCTCTTAA‑TTT‑a                                                                                                                                                                                                                                  >  1:1225525/1‑239 (MQ=255)          tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑Acc                                                                                                                                                                                                                                <  1:824393/239‑1 (MQ=255)              tCGAAAAACTTTGCCATTCCCTGCCTTGGCGCAATTTTCATTCTTATTTTCGCGTTGATTCACAAACTGTTATTTAC‑ATATTCAGGGTGGCAAAAAATAAAAACCCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAAAAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑TTTGTCGCTAAT‑TTT‑ACCGCCa                                                                                                                                                                                                                            <  2:1225525/239‑1 (MQ=255)              tCGAAAAACATTGCCATCCCCTGCTCTGGCGCAATATTCATTCTTATTGTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATAAAAACCCACATTTTTAGGGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCAAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCa                                                                                                                                                                                                                            <  2:1128342/239‑1 (MQ=255)                    aaCATTGCCATTCCCTGCTCTGGCGC‑ATATTCATTCTTATTTTCGCGTTGATTCACGTACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGAGTTTTTCCGTGAAATACAAAAA‑CCAG‑A‑GCTG‑GT‑ATG‑GT‑GATAGGGCGTAATAAGCAACAAGGAGAG‑GGTAAAATGAAAGGG‑GTTGTGCCTAAG‑TTT‑ACCGCCATGAtgta                                                                                                                                                                                                                     >  2:779950/1‑236 (MQ=255)                      cATTGCCATCCCTTGCTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGAAAAAAATATAAACCCACATTTTTAGGGGGAGTTTGGGGC‑GGTAAATTTTTCCAGACAAATACAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCt                                                                                                                                                                                                                    <  1:696702/239‑1 (MQ=255)                            cacccctttctctGGCGCATTATATATTCTGATTACCGAGTTGAACAACGAACTGTTAAATGC‑ATATGCCGGGGGGAAAAAAATATAAATCAACATTTGTAGTGGGATGTTGGGGG‑GGTGATTTTAACAAAAAAAAAAAAAAAA‑CAGG‑A‑GTTT‑CC‑ATA‑AG‑GCGCTAAAAAAATAAGCAACAGACGGAA‑AAAAAGTATACAGAG‑TTTGCCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGa                                                                                                                                                                                                              <  2:318866/230‑1 (MQ=255)                            cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGTTTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAAACAAATACAAAAA‑TTGG‑A‑GTTG‑CC‑ATG‑AT‑AAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTGGAAAGAG‑ATTGTCCCTAAC‑TTT‑ACCGCCATGATTTCTACCCGa                                                                                                                                                                                                              <  1:95797/239‑1 (MQ=255)                                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCAGACAAATACAAAAA‑CAGG‑A‑GGTG‑CC‑CTG‑AT‑GAGAGAAAAAAAAAAACAAAACACAGGG‑GAAAAATTTAAAAGG‑AATTTAGCTCAC‑TTT‑ACCGCCCACATTTTTTCCCGaaag                                                                                                                                                                                                           >  2:634911/1‑236 (MQ=255)                                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGGAAAAAACAAAAA‑CTTG‑C‑GTTG‑CC‑ACT‑CT‑TTGTGCAAGGAATAAGCAACAGGCCGAG‑GATAAGATGGCAGAG‑ATTTTCGGCAAA‑TTT‑ACCGCCATGATTTTTACCCGAAAGCCtgtt                                                                                                                                                                                                     >  1:156371/1‑237 (MQ=255)                                         ggCGAAATATTATTACTTATTACGGGGTTGATACACGAAATTTAAATTCA‑ATATTCTGGGTGGAAAAAAAGATAAAACCATTTTTTAATTGGGAGTTTG‑GGG‑GGGGAATTTTTACGAAAAAAAGCAACAA‑GTGGAA‑GTTT‑CC‑AAG‑AG‑GAGAGAAGTTAAAAGGCAACAGGTAGGA‑AAAAGGTAGACGGAA‑GTTGTCGTAATC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGAc                                                                                                                                                                                                  <  2:1329746/238‑1 (MQ=255)                                           cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCCGACAAATACAAAAA‑ATGG‑A‑GGTG‑GC‑CTG‑AT‑GAGAGAAAGTAATAAAAAAAAAGCAGGG‑AATAAATTTAAAGAG‑AATTTCGCCAAA‑TTT‑ACCGCCATGATTTCTACCCGAATGCCCGATGAAgtg                                                                                                                                                                                               >  1:507303/1‑239 (MQ=255)                                           cgaaaTATTCATTCTTATTTTCGGTTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTAAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgt                                                                                                                                                                                                <  1:1272623/235‑1 (MQ=255)                                            gaaaTATTCATCTTTATTTCGTCGTTGATACACGAA‑TTTTAAATACAATATTCACGGTGACAAAAAATATAAAACCCCATTTTAAGTGGTAGTTCGCGGC‑GGTAATTTTTCCCAAACAAATACAAAAA‑CGGG‑A‑GTTC‑CC‑CTG‑AT‑GAGCGAAAGAAATAAGCACCAGGCAGTG‑AATAAGTTGCCAGAA‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                                               <  2:561957/236‑1 (MQ=255)                                                ttttcattcTTATTTTCGCTTTTATACACGAACTTTTAATTAC‑ATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTAAATTTTTCCAAACAAATACAAAAA‑CGGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACGGGCGGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                          <  2:28948/237‑1 (MQ=255)                                                tatgtattcTTATTGCCTCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGGGGTAGTTTGGGGC‑GGTGAATTTTTCCAGACAAATAAAAAAA‑CTGG‑A‑GTTG‑CC‑TTG‑AG‑GAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGGG‑ATGGCCGTTACC‑TTT‑TCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                          <  1:1342860/234‑1 (MQ=255)                                                tatTCATTTTTATTTTCGCGTTGATTCACGAATTGTTAATTAC‑ATATTCAGGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGAAAAATACAAAAA‑CTGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGAAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                          <  1:7648/239‑1 (MQ=255)                                                tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATT‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑Acc                                                                                                                                                                                                                                <  1:735171/201‑1 (MQ=255)                                                    cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCCGACAAATACAAAAA‑ATGG‑A‑GTTG‑CC‑CTG‑AT‑GAGCGGAAGGAATAAAGAACAAGCAGTG‑AAAAAGATGACAGAG‑AATGTCGCCAAA‑TTT‑AACGCCATGATTTTTAACCGAATGCCTGATGACGTGGGGGGTaaa                                                                                                                                                                                      >  1:561957/1‑239 (MQ=255)                                                     attcTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAAACAAAAACAAAAA‑ATGG‑A‑GGTT‑CC‑CTG‑AT‑GAGGGAAAATAATAAGCAAAAAGCAAGG‑AATAAATTGTCAGAG‑AATGTCGCTAAC‑TTT‑AACCCCATGCTTTTTACCCGAAAGCCCGGTGACGggggggggaaa                                                                                                                                                                                      >  2:1272623/1‑233 (MQ=255)                                                         ttATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                                 <  1:871887/239‑1 (MQ=255)                                                          tATTTTCGCTTTGATTCACGAACTGTTAATTGC‑ATACTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGG‑GGTGAATTTTTCCAAACAAATACAAAAA‑GTGG‑A‑GTTG‑CC‑TTG‑AG‑GAGCAAAAGTAAAAAGCAACGGGCATTA‑AAAAAGTGAACAGAG‑TTTTTCTCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                                <  1:992903/239‑1 (MQ=255)                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGGAATTTTTCAGACAAAAAAAAAAA‑ATAG‑A‑GGTG‑TC‑AAT‑AT‑GTGCGGAAGTAATAAAAAACACGCAGTG‑AATAAAGTTACAGAG‑ATTGTGGCTAAC‑ATT‑ACCGCCCATATTTTTATCCCCATACCTGATAAGAGGGGGGGTAAACAaaaa                                                                                                                                                                                >  1:883698/1‑239 (MQ=255)                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAAATTTTTCAAACAAAAAAAAAAA‑AAGG‑A‑GTTT‑GC‑ATT‑GT‑TGGCGGAAAGAATAAACAAAACGCAATGAAAAAAATTGA‑AGAG‑AATGTTGGTAAA‑CTT‑ACCCCCCTAGTTTTTTCCCGAATTCCCGTTCAAGAGGGGGAGAAACAaaaa                                                                                                                                                                                >  2:1293926/1‑239 (MQ=255)                                                            ttttCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTCCAAACAAATAAAAAAA‑ATGG‑A‑GGTG‑CC‑CTG‑AT‑GAGCGCAAAGAAAAAGCAAAAAGCAGTG‑AATAAGATGAGAGAG‑ATTGTCGGTAAC‑TTT‑TCCGCCCAGAATTCTTCCCCAATGACTCAAGAAGAGGGGGGtaaaataaaaaa                                                                                                                                                                              >  1:1028344/1‑237 (MQ=255)                                                                   gTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTTCCACAAAAAAAAAAA‑ATTG‑A‑GTGT‑CC‑ACT‑GT‑TAGCGGAAATAATAAGCAAAACGAAGCG‑AATAAAATGAGAGAG‑AATTTTGTTAAA‑CTT‑TCCGCCAAGATTTCTACCCCGAAACCTGAGGACGTGGGGGGTAAAATAAAACAGCTaaaa                                                                                                                                                                       >  1:86550/1‑238 (MQ=255)                                                                                 tGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGACAAAAAAAAAAA‑ATGG‑A‑GGTG‑CC‑ATG‑AT‑TAGGGAAAGGAATAAGCAAAAAGCAGGG‑AATTAATTGACAGAG‑ATTGTTTCTAAC‑TTT‑TCCGCCCTGATTTTTACCCCCATGCCTGATGAAGTGGGGGGTAAACTTAAAAAGCTAAAGGATGCCGGAAcac                                                                                                                                                          >  1:1194302/1‑236 (MQ=255)                                                                                   ttAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGAATTTTTCAAAACAAAAAAAAAAA‑TGGG‑G‑GTTG‑‑C‑ATG‑ATGGGGGGAAGGAAAAAAAAAAAAGGCAG‑G‑AATAAATTGAAAGAGAATTTTGGTTAAT‑TTT‑ACCACAATATTTTTTACTGAAATG‑CTGATAGAGTGGGGGGTAAAACAAAAAAGATAAAGGATTCCGaaaaagag                                                                                                                                                        >  2:273912/1‑233 (MQ=255)                                                                                   ttAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                                <  1:303499/214‑1 (MQ=255)                                                                                      aTTAC‑ATATCCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGG‑GGTGAATTTTTCAAGAAAAATAAAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AG‑GGGCGAAAGTAATAAGCAACGGGAAGTG‑AATAAGTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGa                                                                                                                                                    <  1:674231/239‑1 (MQ=255)                                                                                            atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGACAAATACAAAAA‑CTTG‑G‑GTTT‑CC‑ATG‑AT‑GAGCGAAAGAAATAAAAAAAAGGCATTG‑AGTTAAATGACAGCA‑ATTATCGCTTAC‑TTT‑ACCCCCATGATTTTTACCCGAATGCCCTCTGACGTGGTGGGTAAAATAAAAAAGATAAAGGATGCCGAAAAGTAGTCGATgggg                                                                                                                                               >  1:707493/1‑239 (MQ=255)                                                                                            atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCAGACCAAAACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGGGAAAAGAATAAACAAAAGGCAGTG‑AAAAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ATCGCCACGCTTTCTACCCCAAAGCCTGATGACGTGGGGGGTAAAATAAAAAAGCTAAAAGATGCCCAAACATCGTCCGTgggg                                                                                                                                               >  2:774665/1‑239 (MQ=255)                                                                                             tatTCACGTGTGCAAAAAATATAAAACCTATTTTTTAGTGGGAGTTTGTGGC‑GTTGAATTTTCCCAGACAAAAACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACGGGCAGTT‑AATAAGTTGACAGAG‑ATTGTCTCTACC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                              <  1:480142/239‑1 (MQ=255)                                                                                             tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                              <  1:1166729/239‑1 (MQ=255)                                                                                             tatTCACGGGGGAAAAAAATATAAAACCACTTTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CGGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGAAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                              <  2:772923/239‑1 (MQ=255)                                                                                              atTGACGGTGGCAAAAAATACAAAACCACATTTTTAGTGGTAGTTTGGGGC‑GGTGAATTTTTCCAGACAAATAAAAAAG‑CTGG‑A‑GTTG‑CT‑AGG‑AT‑GAGCGAAAGTAATAAGCAAGAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGaa                                                                                                                                             <  2:86550/239‑1 (MQ=255)                                                                                              atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAGACAAAAACAAAAA‑CTGG‑A‑GGTG‑CC‑ATG‑AT‑GAGCGGAAGTAAAAAACAAAAAGCAGGG‑AATAAATTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCCTGATTTCTACCCGAATGCCCGATGACGTGGGGGGTAAACTAAAAAAACTAAAAGATGGCGAAACGTCGTCGGTggggga                                                                                                                                             >  2:896696/1‑237 (MQ=255)                                                                                              atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTTCAAACAAATACAAAAA‑ATGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAATTAAAAGCAAAAAGCAGTG‑AATAAGATGAGAGAG‑ATTGTCGCTAAC‑ATT‑TACGCCATGCTTTTTAACCGGATACCTGATGAAGGGGGGGGTAAAAAAAAACAGCTAAAGGAGGCCGAAACGGCGTCGCTggggga                                                                                                                                             >  1:48406/1‑237 (MQ=255)                                                                                               tgcagggTGGAAAAAAATATAAAACCACTTGGTTGGGGGTAGTTGGTGGC‑GGTGAATTTTTCAAGAAAAAAAAAAAAA‑CGGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAAGAGGAAGGA‑AGTAGGTTGAAGGAG‑ATTGTCGATAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                              <  1:127005/232‑1 (MQ=255)                                                                                                tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGGGAAATTTTTCAAACAAAAAAAAAAA‑AAGG‑AGGTTG‑CC‑‑TG‑AT‑GAGGGGAAAAAATAAACAACAAGAAAGG‑AATAAGATGATCGAG‑ATTGTCGCCAAC‑ATT‑TCCGCCCCAATTTTTACCCGAAAGGCTGATGACGTTGGGGGGAAAATCAAACCGCTAAAAGAAGCCGAAAAATCGGCGGTTGGGaaaa                                                                                                                                           >  2:191838/1‑239 (MQ=255)                                                                                                  tgggTGGAAAAAAATATAAAACCACATGTTTAGTGGGAGTTTGTGGG‑GGTGAATTTTTCAAGACAAATAAAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑GT‑GAGAGAAAGTAATAAGCAACAGGCAGTG‑AATAATTTGACAGAA‑ATTGGCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAAtt                                                                                                                                         <  1:301350/237‑1 (MQ=255)                                                                                                    gggggCAAAAAATATAAAACCACATGTTTAGTGGTAGTTTGTGGC‑GATGATTTTTTCCAGAAAAACACAAAAG‑CTGG‑G‑TTTG‑CG‑ATG‑AT‑GGGCCAAAGTAATAAGCAACAGGCAGTG‑AATAAGTGGACAGAG‑ATTGGCGCTAAC‑TTG‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                                       <  1:779950/236‑1 (MQ=255)                                                                                                     gggacAAAAAATAAAAAACCCTCTTTTTAGTGGTATTTTGTGGG‑GGTGAAATTTTACAGAAAAATCAAAAAG‑CGTG‑A‑TGTG‑CA‑ATG‑AT‑GGGCAAAAATAATAAACATTAGGCAGTT‑AAGAAGTTGACGGAT‑ATTGTCTTTAAC‑TCT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                                       <  2:1046192/234‑1 (MQ=255)                                                                                                     ggagaaaaaaaTATAAAACCAAATTTTTAGTGTTATTTTGGGGC‑GGTAAATTTTTCAAGACAAATAAAAAAA‑CTGG‑A‑GTGG‑CC‑ATG‑AG‑GAGCAAAAGTAAAAACCAAGAGGAATTA‑AATAAGTTGAAGGAG‑ATTGCCG‑TAACTTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                                                                                                                       <  2:1028344/233‑1 (MQ=255)                                                                                                      tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCCCAGAAAAATAAAAA‑ATTG‑A‑GGTG‑CA‑ACG‑AT‑AAGGGAAAGAAATAATTCACAGGCAGGG‑ACTAAGTTGTTAGAG‑AATGTTGCTAAC‑CTT‑ACCGACATTGTTTATACCCGAAAGCCTTATTACGTGGTGGAAAAACTAAAAAACCTAAACGAAGTCCAAACGTGGTCGGTGGGGAAAATTATc                                                                                                                                      >  1:1298972/1‑238 (MQ=255)                                                                                                         cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAAATTTTTCAGAAAAATAAAAAAA‑ATGG‑A‑GGTG‑TC‑ATG‑AT‑GAGCGAAAGTAAAAAAAAAAAAGCAGGG‑AATAAGATGACAGAG‑ATTGGCGCTAAA‑TTT‑ACCGCCATGATTTTTACCCGAATGGCTGATGACGTGGGGGGTAAACTAAAACAGCTAAAAGGAGCCCAAAAGTCGTCGATGGGGGAAAATATCTAcc                                                                                                                                  >  1:949350/1‑239 (MQ=255)                                                                                                              aaaataAACCCAATTTTTTAGGGTTAGTTTGGGGC‑GGTGAATTTATCAAGAAAAAAAAAAAAA‑TTGG‑A‑GTGG‑CT‑ATG‑AT‑GAGCGAAAGTAATAAGCAACGGGCAGTA‑AATAAGACGAGAGGA‑TTTGCCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                                             <  1:429396/236‑1 (MQ=255)                                                                                                              aaTAAAAAACCACATTTTTAGTGGTAGTTTGGGGC‑GGTGAATTTTTCCAGAAAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑AGG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTAACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACg                                                                                                                             <  2:1194302/239‑1 (MQ=255)                                                                                                                          caTTTTTAGTGGTAGTTTGTGGC‑GGTGGATTTTTTCAAACAAAAACAAAAA‑ATTG‑G‑GGTT‑TC‑ATG‑GT‑GAGGGGAAGTAAAAAACAAAAGGCAGAG‑AAAAAATTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCCTGATTTTTTCCCCCAAGCCTGATGACGGGGGGGGTAAAATAAAAAAACTAAAAGAAGACGAAAAGGCGTCGGTGGGGGAAAATATTTACCACACGATGTTTGacaac                                                                                                                 >  2:531321/1‑239 (MQ=255)                                                                                                                                       gtttgggggC‑GGTGAATTTTTCCAGAAAAATACAAAAG‑CTGG‑A‑GTGG‑CC‑ATG‑AT‑GAGCAAAAGTAATAAGCAACAGGCAGTG‑AAAAAGTGGCAAAAG‑TTTGTCCTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTTCCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                                    <  1:88424/232‑1 (MQ=255)                                                                                                                                       atttggtgGC‑GGTGAATTTTTCCAAAAAAATACAAAAC‑CTGG‑A‑GTAT‑CC‑ATG‑AT‑GGACGAAATAAAAAACAAACAGGCATTG‑AATAAGTTATCATAT‑ATTTTCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                                    <  2:707493/234‑1 (MQ=255)                                                                                                                                       aGTTTGTGGG‑GGTAAATTTTCCAAAAAAAATACAAAAA‑CTGG‑A‑GTTG‑‑C‑ATGAAG‑GAGCAAAATTAAAAAGCAACAGGCAGTA‑AATAAGTGAACAGAG‑ATTGTCGCTAAC‑TTG‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                                    <  2:1019870/239‑1 (MQ=255)                                                                                                                                       aGTTTGTGGC‑GGTGAATTTTTCCAGACCAATACAAAAA‑CTGG‑A‑GGTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAACAAAAGGCAGTG‑AATAAGATGACAGAG‑ATTGTTGCTAAC‑TTT‑ACCCCCATGCTTTCTACCCGAAAGCCTGATGACGTGGTGGATAAACTAAAAAAGCTAAAGGATGCCGAAAAGTCGTCGGTGGGGAAAAATATCTACCATAAAATGTTTGACAACATACAAAAAACGa                                                                                                    >  1:1000310/1‑239 (MQ=255)                                                                                                                                       aGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑TTTG‑CA‑AGG‑AG‑GAGCGAAAGTAAGAAGCAACAGGCGGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                                    <  2:117489/239‑1 (MQ=255)                                                                                                                                          ttGGGGC‑GGTGAATTTTTCCAGAAAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt                                                                                                  <  1:284448/238‑1 (MQ=255)                                                                                                                                             tgGC‑GGTAAATTTTCCCAGACAAATAAAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑AAGCGAAAGAAAAAACCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                                               <  1:272301/238‑1 (MQ=255)                                                                                                                                                  ggTAAATTTTTCCAAAAAAATAAAAAAA‑CGGG‑A‑GTTG‑CA‑ATG‑AT‑GAGCAAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGa                                                                                           <  1:531321/238‑1 (MQ=255)                                                                                                                                                          tttCAAGAAAAAAAAAAAAA‑CTGG‑G‑GTTG‑CC‑ATG‑AT‑GAGCAAAAGAAATAAGCAACAGGAAGTG‑AATAAGTTGACAGGA‑ATTGCCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                                  <  2:945321/239‑1 (MQ=255)                                                                                                                                                           tttcaaaaaaaaACAAAAAGCTGG‑A‑GTGC‑GA‑ATG‑AT‑GAGAGAAAGGAATAAAAAACAGGCGGTG‑AAAAAGTGGACAGAG‑TGTGTCGTTACC‑TTT‑CCCGCCAGGATTTCGACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                                  <  2:713947/231‑1 (MQ=255)                                                                                                                                                            acagaaCAAATACAAAAA‑CTGG‑‑‑GTTG‑CCAATA‑AG‑GAGCGAAAGAAATAAGCAACAGGCAGAA‑AATAAGTTAAAAGAG‑ATTGTCGC‑AAC‑TTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctgcc                                                                                <  2:251238/234‑1 (MQ=255)                                                                                                                                                                 aaaaaTAAAAAAA‑CTAG‑‑‑GTTGCCC‑ATG‑AT‑GAGAAAAATGAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCa                                                                           <  2:527294/237‑1 (MQ=255)                                                                                                                                                                  aaaaaACAAAAA‑TGGG‑A‑GTTG‑CC‑ATG‑AA‑GAGGAAAAGGAAAAAGCAACGGGAAGTA‑AAAAAGTGAACAGAG‑ATTGTCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                                                            <  2:59070/236‑1 (MQ=255)                                                                                                                                                                   aaaTACAAAAA‑CGGG‑A‑GTTG‑CC‑ATA‑AT‑GAGGAAAAGTAATAAGAAACAGGCAGTA‑AATAAGTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAgg                                                                         <  1:1293926/239‑1 (MQ=255)                                                                                                                                                                    aaTAAAAAAC‑CTGG‑A‑GTGG‑CC‑ATG‑AG‑GAGCGAAAGAAATAAGAAACAGGCAGTG‑AAAAATTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGa                                                                        <  2:949350/239‑1 (MQ=255)                                                                                                                                                                          aaaa‑CTGG‑A‑GTTG‑CC‑AGG‑AT‑GAGCAAAAATAATAAGCAAGAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTACT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgg                                                                  <  2:507303/239‑1 (MQ=255)                                                                                                                                                                          aaaa‑CGGG‑A‑GTTT‑CC‑AGG‑AG‑GAGCAAAATTAATAAGCAACAGGCAGTA‑AAAAAGTTGACAGAG‑ATTGTCGCTAAC‑TTA‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgg                                                                  <  1:509066/239‑1 (MQ=255)                                                                                                                                                                            aa‑CTGG‑A‑TTGG‑CC‑ATG‑AT‑GAGCAAAAGTAAAAAGCAAAAGGAAGTG‑AATAAGTTGAAAGGT‑ATGGCCGCTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                                <  1:981200/239‑1 (MQ=255)                                                                                                                                                                            aa‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTA‑AATAAGTTGACAGAG‑ATTGTCGCTACC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                                <  2:792659/239‑1 (MQ=255)                                                                                                                                                                            aa‑CGGG‑T‑GTGG‑CC‑ATG‑AT‑GAGGAAAAATAATAAACAACAGGCAGTG‑AATAAGTTGAAGGAG‑ATTGCCGTAAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                                <  2:395858/239‑1 (MQ=255)                                                                                                                                                                             a‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACGCCGGGga                                                               >  1:798271/1‑239 (MQ=255)                                                                                                                                                                                  g‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGAtt                                                           >  2:204027/1‑239 (MQ=255)                                                                                                                                                                                         g‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                                       >  1:1009050/1‑238 (MQ=255)                                                                                                                                                                                         g‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                                                       >  1:1270539/1‑238 (MQ=255)                                                                                                                                                                                            c‑ATG‑AT‑GAGGAAAAGTAATAAGAAACTGTCAGTG‑AATAAGTTGACAGAG‑ATGGTCGTTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                                                     <  1:176245/238‑1 (MQ=255)                                                                                                                                                                                            c‑ATG‑AT‑GAGCGAAAGTAAAAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGCCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                                                     <  1:896696/238‑1 (MQ=255)                                                                                                                                                                                             aatg‑ag‑gAGCAAAGATAAAAAGCAACGGGCAGGG‑AATAAGTTGCAAGAG‑ATTGCGGCAAAT‑TTA‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTGCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                                                     <  2:740454/237‑1 (MQ=255)                                                                                                                                                                                              aagggt‑gaGCAAAAGAAATAAGAAACAGGCAGTG‑AAGAAGTTGAAAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttc                                                     <  2:740417/233‑1 (MQ=255)                                                                                                                                                                                                  at‑gaGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                                               >  2:73660/1‑96 (MQ=255)                                                                                                                                                                                                  at‑gaGCAAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                                                               <  1:73660/96‑1 (MQ=255)                                                                                                                                                                                                     gggggaaagaaaAAAACACAAGGCATTA‑AATATGTTGAAGGAG‑CTGGTCTATAAT‑TTT‑CCCGCCATGATTTCTACCCGAATGCCTGATGACGTTGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTCCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGtt                                               <  2:1260604/234‑1 (MQ=255)                                                                                                                                                                                                               aaaaagaacaaGGCATTG‑AATAAGTAGACAGTG‑ATTGCCGTGAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGt                                      <  2:798271/227‑1 (MQ=255)                                                                                                                                                                                                                 taaGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTccc                                  >  1:1133048/1‑239 (MQ=255)                                                                                                                                                                                                                    gCAACAGAGAATG‑AAAAAGGTGACAGAG‑TGTGTCACTACC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTGCGTTAAAGTCGGTTCCCg                                 <  1:811517/237‑1 (MQ=255)                                                                                                                                                                                                                    agaagtgtaaGTG‑AAAAATTTGACAGTG‑ATGGTCTCAGAC‑TTG‑ACCGCCATGATTTCTCCCCGAATGCCGGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCt                               <  2:136725/230‑1 (MQ=255)                                                                                                                                                                                                                        cAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                           >  2:913483/1‑239 (MQ=255)                                                                                                                                                                                                                        cAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                            >  2:985981/1‑238 (MQ=255)                                                                                                                                                                                                                        cAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                            >  2:663346/1‑238 (MQ=255)                                                                                                                                                                                                                        cAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGAGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGTTTCCCGCTTccc                           >  2:1169885/1‑239 (MQ=255)                                                                                                                                                                                                                         aGGCATTT‑AAAAATTTGACAGAG‑TTTGTCACTACT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa                          <  1:748529/239‑1 (MQ=255)                                                                                                                                                                                                                         aGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                           >  2:926342/1‑238 (MQ=255)                                                                                                                                                                                                                         aGGCAGTG‑AAAAAGTTAACAGAG‑TTTTGCGTTAAT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa                          <  1:391365/239‑1 (MQ=255)                                                                                                                                                                                                                               tG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCtt                    >  1:1348656/1‑239 (MQ=255)                                                                                                                                                                                                                                    taattggaaagaa‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg                <  1:191838/230‑1 (MQ=255)                                                                                                                                                                                                                                    tAAGTTGACAGAG‑TTTGCCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg                <  1:935415/239‑1 (MQ=255)                                                                                                                                                                                                                                    aaaGTTGACAGAA‑TTTGCCGC‑AAC‑TTTGACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg                <  1:305445/238‑1 (MQ=255)                                                                                                                                                                                                                                          gACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc          >  2:415867/1‑239 (MQ=255)                                                                                                                                                                                                                                               ag‑aTTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGAGAGCTGCAAAGc     >  2:265666/1‑239 (MQ=255)                                                                                                                                                                                                                                                  aTTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCAt   >  2:1203217/1‑239 (MQ=255)                                                                                                                                                                                                                                                   tgggcGCTACT‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATa  <  2:503738/235‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           AGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAC‑ATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGC‑GGTGAATTTTTCCAGACAAATACAAAAA‑CTGG‑A‑GTTG‑CC‑ATG‑AT‑GAGCGAAAGTAATAAGCAACAGGCAGTG‑AATAAGTTGACAGAG‑ATTGTCGCTAAC‑TTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAAAGCATA  >  NC_000913/3206281‑3206750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑