| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,091,193 | T→A | 19.3% | I392F (ATT→TTT) | frvB ← | putative PTS enzyme, IIB component/IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,091,193 | 0 | T | A | 19.3% | 163.6 / 8.7 | 76 | I392F (ATT→TTT) | frvB | putative PTS enzyme, IIB component/IIC component |
| Reads supporting (aligned to +/- strand): ref base T (30/28); new base A (12/3); total (44/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCATCGCA > NC_000913/4090958‑4091431 | cggcgAAACCACAATCCTTCCAGGACGATAAGGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGAAAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGTGCCAGACGTTTGATCCCG‑AAGGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAg < 2:603093/238‑1 (MQ=255) ggcgTAACCCCAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGCCGTTTGATTCCG‑AAGGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGc < 1:1034692/238‑1 (MQ=255) gTACCCCCAATCCTTCCAGGAGAATAATGATTGAAATGTTCCCCCCGCGGCCAACCGCCAGCCCCAAAAAAAACGAACCCCCCGCGGACACCAGCCCGATCGGCGGTTCAAAAATCCCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGACCACCAGCA‑CGCCGGAAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCcggt < 2:993940/239‑1 (MQ=255) tAACCACAATCCTTTCAGGACGAAAAGGAAGGAAATGTTAAGCGCGAGGCAAACCGCCAGCACCAGATAAAACGACCCCACCGGCGACATCAGCCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGGGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCcggt < 2:360662/238‑1 (MQ=255) aaTCCTTCCAGGACGTAAATGAAGAAAATGTTCAGCGGGGGGCCAATCCCCAGCACCAAATAAAACGAACCCACCGGCGAAAACCGGCCGATCCGCGGGTAAAAATCACCCAAACCA‑GGTCCCGGCCGTTGGATTCCG‑AAAGCGATCCCCGGCA‑CGCCGTTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGc < 2:1127668/239‑1 (MQ=255) aTCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCACGGAGCTGCCCGGGGGGAA‑GGAAAAACcgggtcgggttgg > 2:86710/1‑234 (MQ=255) tttCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGCAATCATCGGCAACAGGACTGCCAGCGCGTAA‑GGAAAAGCCGATTCTGTTGCGGCtg > 2:761677/1‑239 (MQ=255) tCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGCGCCGCCCGAGCGGAA‑GGAATAAACAGTTCGGGTgaggccgtg > 2:507642/1‑231 (MQ=255) cGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCAcc < 2:130620/238‑1 (MQ=255) gATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAACGAGCTGCCAGGGGGCAA‑GGAAGAGCCGGGTCGGCTGCGAGTGGGGCACCg > 1:1043625/1‑238 (MQ=255) gATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATACTCGGCAACGGAGGTGCCCGAGCGGAA‑GGAAAAGCCGGTTCGGTTGCGGCCTTGGCCCCg > 1:1062046/1‑238 (MQ=255) tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGGAA‑GGAATAGc > 1:153950/1‑211 (MQ=255) tAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGGCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATAAACCGCAACGGAGACGCCGGAGCGTAA‑GGAATAGCCGGTTCGGGTGCGGCAGTgggcccagac > 2:406278/1‑231 (MQ=255) atgaatgaAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACcac < 1:1058207/239‑1 (MQ=255) atgaaATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATg < 1:897649/239‑1 (MQ=255) aaaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTAAAAGATCCCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATgg < 1:1155817/237‑1 (MQ=255) aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAAGGAGCTGCCCGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCa > 1:431799/1‑238 (MQ=255) aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGAAATCATCGGCAACGGAGCTGCCCGAGCGGAA‑GGAAAAGCCGGGTTGGGTGGGACTGTGGGGCCCACcacacatgga > 1:512837/1‑232 (MQ=255) aaTGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCACGCCAGCGTATTGGCGGCACGCATCCGCGACCGAGCTGACCGAGCGGAAGGGAAAACCCGGTT‑GGGTGCGACTGGGGCGCCGGCCACACGGGCa > 2:269630/1‑238 (MQ=255) gTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGCAATCATCGGCAAAGGGGCCGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAAAGGCacta > 1:856099/1‑238 (MQ=255) tCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAAACATCGGCAAAGGAGCTGCCCGGGCGTAG‑GGAAAAGCCGGGTGGGTTGGGGCCGTGGCACCGGACAAAATgggccaaaag > 1:1097077/1‑231 (MQ=255) cAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAACGGAGCAGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTTCGACTGGGGCCCCGAACACAATGGGACCACTg > 1:1323707/1‑238 (MQ=255) aGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAAACATCGGCAACGGAGCTGCCCGCGCGGAA‑GGAATAGCCGGTTCGGGTGGGGCTGTGGCACCGACCACAAAGGCACTACCgg > 2:278101/1‑238 (MQ=255) cgcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCCACGGAGCGGCCAGAGCGTAA‑GGAAGAACCGGGTCGGGTGGGACCGTGGGACCGGCCCCCAAGGCCCTCCTGGCa > 2:754034/1‑238 (MQ=255) cgcgAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCACGGAGCTGCCAGAGCGGAA‑GGAAAAACCGGGTCGGGTGCGGCCGGGGGCCCGCACACAAAGGCACCACTGGCa > 2:1155817/1‑238 (MQ=255) gcaagccaaaTCGCCAGCCCCAGAAAAAACGCACCCCCCGGCGACAACAGGCCGATCAGCGGGTCAAAGATCCCCAAACCA‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGACCACCAGCC‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAg < 2:1300141/230‑1 (MQ=255) ggCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAAAGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGGGACTGTGGCACCGACCACACAGGGACTACTgggcgccact > 1:1168749/1‑231 (MQ=255) cGCCAGCACCAGATAAAACGAACCCGCCGGCGACATCAGGCCGACCAGCGGGTCAAAGACACCCAAACCA‑GGTGCCAGACTTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCt < 2:1054994/239‑1 (MQ=255) ccacccccAGATAAAACGACCCCACCGGGGACATCGGGCCGACCGGGGGGTCAAAGATCCCCAACCCA‑GTTGCCAGACGTTTGATCCCA‑AAAGCGACCCCCACCA‑CGCCGTTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGt < 2:512837/233‑1 (MQ=255) cAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGGTCGGTTGCGACTGTGGCACCGACCACAAAGGCACCACTGGCAGCCTCTTTTTCCTGTc > 1:280667/1‑239 (MQ=255) aGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCCGAGCGGAA‑GGAATAGCCGGTTTGGGTGCGACTGGGGCACCGACCCCAATGGGACTACACGCAGCCTCTTTTTcccttcg > 2:175343/1‑234 (MQ=255) gCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATTCTCCGCCACGGAGCTGCCAGAGCGGAA‑AGGATAACCGGGTCGGTTGCGACTGGGGCAACGGCCACAAAGGCACTACTGGCAACCTCTTTTTCCTGTCgg > 2:594096/1‑239 (MQ=255) acaaGAAAAAACAAACCCACCGGCGACATCAGGCCAATCGGCGGGTAAAAGAT‑CCCAAACCAGGGTGCCAGACGTTTGATCCCG‑AAAGCGATCCCCAGCA‑CGCGGGTAAGGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCg < 2:569369/233‑1 (MQ=255) ccAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTa < 1:603688/239‑1 (MQ=255) gATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCACGGAGCTGCCAGAGCGGAA‑GGAAAAACCGGTTCGGTTGCGACTGTGGCACCGCCCACCATGGCACTAATGGCAGCCTCCTTTTTCTGTCGGGTAAAc > 1:670224/1‑239 (MQ=255) caaaaaaCGAACCCCCCGGAGACAGCAGGCCGCACAGGGGGTAAAAGATCCCCAACCCG‑GGTGCCAGCCGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc < 2:1062046/236‑1 (MQ=255) tAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAAc < 1:618769/237‑1 (MQ=255) aaCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCACGGGGCTGCCAGGGCGGAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCGCCGACCACCAAGGCACCACTCGCCGCCCCCTTTTTCTGTCTGGTAAAAAGTTTCGgc > 2:314843/1‑239 (MQ=255) aCCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGgc > 1:368111/1‑238 (MQ=255) ccGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAAGGAGCTGCCCGAGCGTAA‑GGAAAAACCGGGTCGGTTGCGACTGTGGGACCGACCACAATGGGAACACTGGCGGCCTCTTTTTTCTGTCGGGGAAACAGATTTGGCGCAATg > 2:529782/1‑239 (MQ=255) ggCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGaa < 1:86710/239‑1 (MQ=255) gACATCAGGCCGACCAGCGGGCCAAAGATACCCAACCCG‑GGTGCCAGAGGTTTGATCCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑g < 2:724574/238‑1 (MQ=255) cATCAGGCCGATCAGCGGGTAAAAGATCCCCAACCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGAc < 2:397133/174‑1 (MQ=255) caccggggggTAAAAGAT‑CCCAAAGCC‑GGTCTCAGGTCTTTTATTCCGAAAAGCG‑CCCCCAGCCCCGCCGTTAATCCCGCCCGCCGGCGTATTGGCGGTAATCATGGGCAACGGAGCTCCCAGAGCGTAA‑GAAATAGCCGGTTCGTTTGCGACTGTGGCCCCGACCACAATGGCACTCCTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTGGGGCGCAATGAAC‑GTCGCAAGACccg < 2:1291617/232‑1 (MQ=255) aTCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCCAACGAGCTGCCAGAGCGGAA‑GGAATAGCCGGGTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGGCCCTTTTTTCCGTCGGGGAAACAGGTTTGGCGCAATGAAC‑ATCCCAAGAaccc > 2:742344/1‑237 (MQ=255) aTCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGGTCGGTTGCGACTGTGGCACCGACCACAATGGCa > 1:397133/1‑174 (MQ=255) cGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTTGGCGCAATGAAC‑GTCGCAAAACCCGCcgcg > 1:938951/1‑238 (MQ=255) ggTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATTCTCCGCAACGGAGCCGCCCAAGCGTAA‑GGGAAAGACGGTTCCGCTGCGACCGCGGCACCGACCCCAAATGCACCACCGGCAGCCTCTTTCTTCTGTCCGGTAAAACGATCGGGCGCCATGACC‑GTCGCACTACCcgccgcccac > 2:1060536/1‑235 (MQ=255) cccAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGGAATCATCGGCCACGGAGCTGCCCGAGCGGAA‑GGAAAAGCCGGTTCGGGTGCGACTGTGGCACCGACCACAATGGCACTACCTGCAGCCCCTTTTTTCTGTTCGGTGAACAGGTTTGGCGCAAAGAAC‑GGCGCCAGACCCCCCCCGTCgggggggctga > 2:1039856/1‑233 (MQ=255) aCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGGTTTGGCGCCATGAAC‑GTCGCCAGACCCGCCGCGACGGGCGGCATCAGcgcg > 1:23870/1‑238 (MQ=255) aCCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGGAA‑GGAATAGGCGGTTCGGGTGCGACTGTGGGACCGACCACAATGGCACTACTGGCAGCCTCCTTTTTCTGTCGGGGAAACAAGTTTGGCGCAAATAAC‑CGCGCCAAGCCCGGCGCGACGGGCGGCagacggcgg > 2:65039/1‑230 (MQ=255) tGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAACGGAGCTGCCAGAGCGTAA‑GGAAAAACCGGGTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCCCTTTTTTCTGTCGGGGAAACAGTTTGGGCGCAACGAAC‑GGCGCCAAACCCGCCCCGGCGGGCGGCCTCAGGCCGACAAAGcccgcgcagggg > 2:1145576/1‑229 (MQ=255) g‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCCGCAACGGAGCTGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGTTTGGGCGCAATGAAC‑GTCGCCAGACCCCCCCCGACGGGCGGCCTCCGCGCGACCACGCCGCCCATGGCGTACCac > 2:68690/1‑237 (MQ=255) aagggcacccccAGCC‑CGGCGGAAGGGCCGCCCGCCAGCGTATTGGGGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc < 1:68690/229‑1 (MQ=255) aaaGCGACCCCCGGCC‑CGCGGGTAGGCCCGCCCGCCAGCGTATGGGCGGTAATCACCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCGGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTGGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTc < 1:622097/239‑1 (MQ=255) gATCCCGAGCA‑CGCGGGTAATGCCCCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATaaa < 1:742344/239‑1 (MQ=255) cA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCCGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGGTTGGGCGCAATGAAA‑GGCGCAAAACCCGCCCCGGCGGGCGGCAACACGGCGCACACCCCGCCCACGGCGGACCAGCCATAAAAGAGttttt > 2:1257764/1‑239 (MQ=255) acgcggtaaaggccgccCGCCAGCGTTTTGGGGGTAAGCAGCGGCAACGGGGCTGCCAGGGGTTAA‑GAAGTAGCGGGTGCGGTTGCGAGTGGGGCACCGCCCAAAATGGCCATACTGGCAGCCTCTTTTCCCTGTGGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTc < 2:1142804/228‑1 (MQ=255) cGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTTCTGTCGGGGAAAAAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCcagc > 1:342983/1‑239 (MQ=255) gcccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGGAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGGAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGGACCAGTCATAAATGTGTTTTTTCAGCAGTGAGAAGc > 1:349611/1‑239 (MQ=255) cccgccAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCCCAATGGCACTACTGGCAGCCTCTTTTTCCTTTCGGGGAAACCGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCCTAAATGTGTTTTTCCAGCAGTGCGAAGCa > 1:354151/1‑239 (MQ=255) ccgcgggggTATGGGGGGAAACCGTCGGCAACGGGCCTGCCAGGGCGTAA‑GGAATGGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCCCTACTGGCAGCCTTTTTTTCCTGTCGGGAAAACAGTTTGGGCGAAATGAAC‑GCCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAGGCag < 2:670224/231‑1 (MQ=255) ttttGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGAAA‑GAAATAGCCGGTTCGTTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCa < 1:1232954/236‑1 (MQ=255) agtGGCGGAAATCACCGGCAAGGGGGCTGCCAGGGCGGAA‑GAAAAGGCGGGTTCGGTGGCGACTGTGCCCCCACCCAAAAGGGCCCTACGGGCGCCTTCTTTTCCCTGTGGGAAAAACAGTTGGGCCGAAATGAAC‑GTCGCAAGCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg < 2:288620/237‑1 (MQ=255) aTTGGCGGTAATCATCGGCAACGGGGCTGCCAGAGCGTAA‑GGAATGGCGGGTTCGGTTGCGACTGTGGCCCCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg < 1:754034/239‑1 (MQ=255) aTTGGCGGTAATCACCGCCAACGGAGCGGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTGGCGACGGTGGCACCGACCACAATGGCACTACTGGCGCCCTCTTTTTCCTGTCGGATAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAgg < 1:314843/239‑1 (MQ=255) gaaTCACCGGCAACGGAGCGGCAAGAGCGGAG‑AGAAAAGCCGGTTCGGTGGCGACTGTGGCACCGACCACAAGGCCACTTGTGGCAGCCTCTTTTCCCGGTCGGGTAAACAGTTTGGGCG‑AAAGAACGGCCGCAAGCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGtttt < 2:703682/237‑1 (MQ=255) cctcGGCACGGGAGCTGCAAGAGGGTAA‑GGAAGAGCGGGTGCGGTGGCGACGGCGGCCCCGACCACAAGGGCACTACTGGCAGCCTCTTTTTCCTGCCGGTAAAACAGTTTGGGCGCAAGGACC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGa < 2:1304864/237‑1 (MQ=255) tcGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTTGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATAGCGTCCCCGTCATCAATGTGTTTTTCCAGCAGTGTGAAGCCGAGGCACCAGGCGGATTTCTTGC‑Tg > 2:603339/1‑239 (MQ=255) tcGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑Tg > 1:1194718/1‑239 (MQ=255) aGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGCTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTCTTGATTGGGgccgcc > 2:528106/1‑238 (MQ=255) gCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGCCGGGCGGCATCAGCGCGACAACGCCGAAGATGGCGCACCAGTCATAAATGTGTTTTTCAAGCAGTGAGAAGCAGAAGAACCAGGCGGGTTTATTGT‑TGGGGCCCGCCATATc > 2:1043736/1‑239 (MQ=255) cgaaa‑GGAAAAGCCGGTTCGGTTGCGACGGGGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGAAAGCCCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGc < 1:1012662/235‑1 (MQ=255) a‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAgaagaa > 1:607227/1‑195 (MQ=255) ggAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCa > 2:1094124/1‑238 (MQ=255) aTAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGGCCCTAACGGACCCCCGccatcc > 2:1007969/1‑234 (MQ=255) tAGCCGGTTCGGTTGGGATTGTGGCACCGACCAAAATGGCACTACTGGCCGCCTCTTTTTCCTGGCGGGAAAACAGTTTGGGCGCAATGAAC‑GCCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCATCGCa < 1:295941/239‑1 (MQ=255) | CGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAGCACCAGATAAAACGAACCCACCGGCGACATCAGGCCGATCAGCGGGTCAAAGATACCCAAACCA‑GGTGCCAGACGTTTGATTCCG‑AAAGCGATCACCAGCA‑CGCCGGTAATGCCGCCCGCCAGCGTATTGGCGGTAATCATCGGCAACGGAGCTGCCAGAGCGTAA‑GGAATAGCCGGTTCGGTTGCGACTGTGGCACCGACCACAATGGCACTACTGGCAGCCTCTTTTTCCTGTCGGGTAAACAGTTTGGGCGCAATGAAC‑GTCGCAAGACCCGCCGCGACGGGCGGCATCAGCGCGACAACGCCGACGATGGCGTACCAGTCATAAATGTGTTTTTCCAGCAGTGAGAAGCAGAAGAACCAGGCGGTTTTATTGA‑TGGGGCCGCCCATATCGAACGCCAGCATCGCA > NC_000913/4090958‑4091431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |