BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,412,890	T→G	10.3%	G168G (GGT→GGG)	yjfM →	DUF1190 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,412,890	0	T	G	10.3%	411.4 / 3.5	138	G168G (GGT→GGG)	yjfM	DUF1190 family protein
Reads supporting (aligned to +/- strand): ref base T (35/86); new base G (15/2); total (50/88)
Fisher's exact test for biased strand distribution p-value = 3.77e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

CTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAT  >  NC_000913/4412656‑4413126

cTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAt                                                                                                                                                                                                                                              <  1:499123/238‑1 (MQ=255)

            cAAAACGGCATTTTAAGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCt                                                                                                                                                                                                                                <  2:702798/239‑1 (MQ=255)

                    cATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTc                                                                                                                                                                                                                         <  1:570476/238‑1 (MQ=255)

                        ttATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCa                                                                                                                                                                                                                    <  2:676351/239‑1 (MQ=255)

                           tGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCaaaa                                                                                                                                                                                                                 <  2:81607/239‑1 (MQ=255)

                            gCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGGT‑TACTTCTGGCGCTCCCGTTCTGGCaaaaa                                                                                                                                                                                                                >  1:297342/1‑239 (MQ=255)

                            gCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCaaaaa                                                                                                                                                                                                                >  2:957434/1‑239 (MQ=255)

                                aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCCCCCCGTCTGGCGCAGCCCTTCTGGTGAT‑TACTCCTTGCGCCCCGGCTCTGGCAAAAAagag                                                                                                                                                                                                            >  2:1291910/1‑239 (MQ=255)

                                aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCCGTCTGGCGCCGCACTTCTGGTGAT‑TACTCCTTGCGCTCCGGTTCTGGCAAAAAagag                                                                                                                                                                                                            >  1:492704/1‑239 (MQ=255)

                                aTGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCAGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGTTTTGCGCCCAGTCTCGGGCCGCCATTCTGGTGGT‑TATTCCTGGGGCCCCGTTTCTCTCGACAAagag                                                                                                                                                                                                            >  2:173494/1‑239 (MQ=255)

                                               tGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCCGTCTGGCGCAGCACTTCTGGTGAT‑TTCTCCTTGCGCTCCGGTTTTGGCCAAAAAAAGTCTTACTC‑TTCGggc                                                                                                                                                                                            >  1:341823/1‑239 (MQ=255)

                                               tGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGggc                                                                                                                                                                                            <  1:397185/239‑1 (MQ=255)

                                                gACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGggcg                                                                                                                                                                                           <  1:1024809/239‑1 (MQ=255)

                                                  cTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTc                                                                                                                                                                                                                         <  2:919021/208‑1 (MQ=255)

                                                  cTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTc                                                                                                                                                                                                                         >  1:919021/1‑208 (MQ=255)

                                                  cTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGGTATCCTCTTGG‑GCTCCGGTCTCGGCAAAAAAAAGACTT‑CTC‑TTTCggcggcg                                                                                                                                                                                        >  2:917173/1‑238 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTGAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCCGCACTTCTGGTGAT‑TACTCCTGGCGGTCCGGGTCTGGCAAAAAAGAAGCTTACTC‑TTCCGGCGGCttt                                                                                                                                                                                      >  1:298939/1‑239 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTTCTCTTTTGCTTCGCGCCCAGTCTTGGGCAGCACTTCTGGTGAG‑TGCTCCTGGCGCTCCGGGTCTGGGCAAAAAGAGTCTTACCT‑TTCTggcgcgctt                                                                                                                                                                                      >  2:911382/1‑234 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGGCTTATTC‑TTCTGGGGGCttt                                                                                                                                                                                      >  1:68287/1‑239 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCCGTCTTGCGCAGGCCTTCTTGGGAT‑TTCTTCTTGCGCCCCCGGTTTGGCAAAAAAGGGGCTTACTT‑TTCGGGCGGCttt                                                                                                                                                                                      >  2:25252/1‑239 (MQ=255)

                                                     agcagAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCCGGCTGGGGCAGCACTTCTTGGGAT‑TACTTCTTGCGCTCCGGGTCTGGCAAAAAACGGGCTTAATCTTTCGggcgcttt                                                                                                                                                                                       >  1:1290941/1‑235 (MQ=255)

                                                        agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTac                                                                                                                                                                                    <  1:560577/238‑1 (MQ=255)

                                                        agAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTCCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTac                                                                                                                                                                                    <  2:1207829/238‑1 (MQ=255)

                                                          aaCTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGGCTTCCTC‑TTCGGGCGGCTTTccca                                                                                                                                                                                  >  1:1008235/1‑238 (MQ=255)

                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCaaaa                                                                                                                                                                            >  1:1286267/1‑239 (MQ=255)

                                                               tCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGGTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTTTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTATCACCaaaa                                                                                                                                                                            >  2:1216637/1‑239 (MQ=255)

                                                                cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCaaaaa                                                                                                                                                                           <  1:429072/239‑1 (MQ=255)

                                                                cAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCaaaa                                                                                                                                                                            <  1:30339/238‑1 (MQ=255)

                                                                 aGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCCGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCCGGTCTGGCAAAAAAAAGTCTTACTC‑TTCGGGCGGCTTTACCACCaaaaaa                                                                                                                                                                          >  1:865534/1‑239 (MQ=255)

                                                                    cTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGc                                                                                                                                                                        <  1:697234/238‑1 (MQ=255)

                                                                       aGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTTGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCt                                                                                                                                                                    >  1:736458/1‑239 (MQ=255)

                                                                             aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCCCCCCGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTTGCGCTCCGGTTCTGGCCAAAAAGAGACTTTCTC‑TTCCGGCGGCCTTACCCCCCAAAAACAGGCTACCgct                                                                                                                                                              >  2:745889/1‑237 (MQ=255)

                                                                             aaaaTTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGGTCTGGCCAAACAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGtt                                                                                                                                                              >  1:201940/1‑239 (MQ=255)

                                                                                   gCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTcgc                                                                                                                                                        >  1:400698/1‑239 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTcgcg                                                                                                                                                       <  1:1247517/238‑1 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTcgcg                                                                                                                                                       <  2:444876/238‑1 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTcgcg                                                                                                                                                       <  1:957434/238‑1 (MQ=255)

                                                                                     tactacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTcgcg                                                                                                                                                       <  1:22417/238‑1 (MQ=255)

                                                                                      actacGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCgcggc                                                                                                                                                     <  1:1338129/239‑1 (MQ=255)

                                                                                            aCAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TGCTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTTCCACCAAAAAAGCGTCTACCGTTTTTCGCCGCGGCTAt                                                                                                                                               >  1:61692/1‑239 (MQ=255)

                                                                                             cAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATg                                                                                                                                              <  1:911382/239‑1 (MQ=255)

                                                                                               aTGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGt                                                                                                                                            <  2:94029/239‑1 (MQ=255)

                                                                                               aTGGTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGt                                                                                                                                            <  2:566270/239‑1 (MQ=255)

                                                                                                    gAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGtt                                                                                                                                        <  2:803278/238‑1 (MQ=255)

                                                                                                          agTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTcgcg                                                                                                                                                       <  1:1253959/217‑1 (MQ=255)

                                                                                                            ttGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCCCCCCAGCTGGCGGAGCACTTCTGGTGGT‑TTCTCCTGGCGCTCCGGTTCTGGCCAAAAACAGGCTTTATC‑CTCGGGCGGCTTTACCACCACAAAAACGTCTAACCTTTCTCGCGGGGGCTCTTGTTGTTTTTcccaa                                                                                                                                >  2:752257/1‑235 (MQ=255)

                                                                                                            ttGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAgc                                                                                                                                >  1:830133/1‑238 (MQ=255)

                                                                                                             tGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAgcg                                                                                                                               <  1:968968/238‑1 (MQ=255)

                                                                                                                    cGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTTCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGGT‑TACTCCTGGCGCTCCGGGTCTTGCAAAAAAAAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCCACCGTTTCTCGCGGCGGCTATGGTCGTTCTTTCCACCC‑CCGTgg                                                                                                                       >  2:583013/1‑238 (MQ=255)

                                                                                                                    cGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCCGTCTTGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCCAAAAAGCGCCTACCGTTTCTCTCGGCGGCCATGGTACTTCTTCCACCGC‑CCCTggg                                                                                                                      >  2:1171150/1‑239 (MQ=255)

                                                                                                                    cGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGGGGT‑TACTCCTGGCGCCCCGGTTCTGGCAAAAAAAAGACTTACTC‑TTTTGGCGGCTTTTCCACCCAAAAAGCGGCTACCCTTTTTCGCGGCGGCCCTGGTTGTTCTTCCCACGC‑CCGCggg                                                                                                                      >  2:1121117/1‑239 (MQ=255)

                                                                                                                      gTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTTCTCTTTTGCTTCGCGCCCCGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTTGGGCGGCCTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCCATGGTCGTTCTTCCCACGc‑cccgggg                                                                                                                      >  2:291831/1‑232 (MQ=255)

                                                                                                                      gTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCCGGTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCCTTTGTCGTTCTTTCCACGC‑CCGTggg                                                                                                                      >  2:476193/1‑237 (MQ=255)

                                                                                                                       tCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCa                                                                                                                    <  2:150349/238‑1 (MQ=255)

                                                                                                                           ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTggg                                                                                                               <  2:400698/239‑1 (MQ=255)

                                                                                                                           ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTggg                                                                                                               <  2:407060/239‑1 (MQ=255)

                                                                                                                           ttCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTggg                                                                                                               <  2:488851/239‑1 (MQ=255)

                                                                                                                           ttCTGGATTTTTGTTAAGCCGGGTTATTCCGAAAGGTCGCCATGGGCCGTTTGTTTAAAACAGCCGCGGTTCCTCTTTTGCCTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTggg                                                                                                               <  1:917173/239‑1 (MQ=255)

                                                                                                                             gtGGATTTTTGGTAAGCCGGGTGAGTCGTAAAGAGCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCGCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑GTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGa                                                                                                             <  2:154799/238‑1 (MQ=255)

                                                                                                                                    tttGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAAt                                                                                                      <  1:719210/239‑1 (MQ=255)

                                                                                                                                    tttGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAAt                                                                                                      <  1:803053/239‑1 (MQ=255)

                                                                                                                                         tAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTATCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATg                                                                                                  <  1:613548/238‑1 (MQ=255)

                                                                                                                                         tAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATg                                                                                                  <  1:1140445/238‑1 (MQ=255)

                                                                                                                                          aaGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTTCTCTTTTTCTTCGCCCCCAGTCTGGGGCCGGACTTCTGGGGGT‑TTCTCCTGGCGCTCCCGTTCTGGCAAAAAAGAGTCTTTCTC‑TTTGGGGGGCTTTACCACCACAAAACGGACTACCCGTTTTCCCGGCGGGTCAGGTCGTTCTTCCACCGCACCGCGGGCTTTGGGGGGGcgacaaggca                                                                                                 >  2:252496/1‑230 (MQ=255)

                                                                                                                                           aGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTg                                                                                               <  1:476193/239‑1 (MQ=255)

                                                                                                                                              cGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTgaga                                                                                            <  2:20202/239‑1 (MQ=255)

                                                                                                                                                 gTTATTCGTAAAGATCGCGGTGTGCCGGTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGaca                                                                                          <  2:201940/238‑1 (MQ=255)

                                                                                                                                                   ttttCGTAAAGATCGCGATGGGCCGTTTGTGTATAACCGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACaa                                                                                       <  2:1149533/237‑1 (MQ=255)

                                                                                                                                                     ttCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGt                                                                                                                         <  1:90497/203‑1 (MQ=255)

                                                                                                                                                               tCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCg                                                                           <  1:90534/239‑1 (MQ=255)

                                                                                                                                                                      gcgCCGTTTGTTTATACCAGCGGGGGTTCCTCTTTTGCTTCGCGCTCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGGTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGAt                                                                    <  1:1326622/237‑1 (MQ=255)

                                                                                                                                                                              tgtttATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGAcaca                                                                                        <  1:1050374/211‑1 (MQ=255)

                                                                                                                                                                                          cggcggTTTCTCTTTTGCTTTGCGCCCAGGCTGGCGCAGCACTTCTTGGGGT‑TTCTCCTGGGGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGGTTTTCCACCAAAAAAACGTCTACCGTTTCTCGCGGCGGCTATTGTCGTTCTTTCAGCGC‑CCGCTGGGATTTGGGGGGGTAATACTGCTGAAGCACAACGGTCCTGTGCGCCGGGGTATGGACCATGTcgccccct                                                 >  2:1274617/1‑234 (MQ=255)

                                                                                                                                                                                             cggTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACa                                              <  1:336898/238‑1 (MQ=255)

                                                                                                                                                                                                            ttCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                               <  1:576305/238‑1 (MQ=255)

                                                                                                                                                                                                            ttCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                               <  1:887209/238‑1 (MQ=255)

                                                                                                                                                                                                                cgcCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatc                          >  1:182359/1‑239 (MQ=255)

                                                                                                                                                                                                                cgcCCAGTCTGGCGCAGCACTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAATAAGCGTCTACCGTTTCTCGCGGCGGTTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGGGACACAACGATCCTGTGCGATGGGTTCTGGGTCACTTAGCCCCTTGCCACGGTTTCGACTTTCatat                           >  2:1131187/1‑238 (MQ=255)

                                                                                                                                                                                                                           gcgcAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGa               >  2:768378/1‑239 (MQ=255)

                                                                                                                                                                                                                             gcatcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:251166/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  2:199021/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:414992/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:1291910/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  2:1079133/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:1132238/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:1121117/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  2:830133/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:25252/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  1:952787/238‑1 (MQ=255)

                                                                                                                                                                                                                             gcagcaCTTCTGGGGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGaa              <  2:865534/238‑1 (MQ=255)

                                                                                                                                                                                                                               agcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAAt            <  1:392448/238‑1 (MQ=255)

                                                                                                                                                                                                                                gcaCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAAAGGTTTCGACTTTCATATCATCGCCAATGCAATCt          >  2:1315955/1‑239 (MQ=255)

                                                                                                                                                                                                                                 caCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTa         >  2:389454/1‑239 (MQ=255)

                                                                                                                                                                                                                                  aCTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTa         <  2:736458/238‑1 (MQ=255)

                                                                                                                                                                                                                                   cTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAtt       <  2:1050692/239‑1 (MQ=255)

                                                                                                                                                                                                                                   cTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAtt       <  2:1286267/239‑1 (MQ=255)

                                                                                                                                                                                                                                   cTTCTGGGGAT‑TACTCCGGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAtt       <  2:377342/239‑1 (MQ=255)

                                                                                                                                                                                                                                      cTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGTCCACGGTTTCGACTTTCATATCATCGACAATGAAAACTATTggg    >  2:430198/1‑239 (MQ=255)

                                                                                                                                                                                                                                        ggTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTTCTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt  <  1:252496/239‑1 (MQ=255)

                                                                                                                                                                                                                                        ggTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAt  <  2:1267845/239‑1 (MQ=255)

CTGGAACAACGCCAAAACGGCATTTTATGCCGATGTTCCGAAGAATATGACTCAGCAGAACTGTCAGTCTAAGTACGAAAATTGCTACTACGACAATGTTGAACAGAGTTGGATCCCGGTCGTTTCTGGATTTTTGTTAAGCCGGGTTATTCGTAAAGATCGCGATGAGCCGTTTGTTTATAACAGCGGCGGTTCCTCTTTTGCTTCGCGCCCAGTCTGGCGCAGCACTTCTGGTGAT‑TACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTC‑TTCGGGCGGCTTTACCACCAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGC‑CCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGAT  >  NC_000913/4412656‑4413126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`