BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,544	G→T	10.8%	A271S (GCT→TCT)	thrC →	L‑threonine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544	0	G	T	10.8%	463.3 / 11.3	154	A271S (GCT→TCT)	thrC	L‑threonine synthase
Reads supporting (aligned to +/- strand): ref base G (25/111); new base T (16/2); total (41/113)
Fisher's exact test for biased strand distribution p-value = 5.08e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

GGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTAC  >  NC_000913/4309‑4777

 gTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCCGGGAGACGTTTTAttgttg                                                                                                                                                                                                                                        >  2:1003762/1‑238 (MQ=255)

     aGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                                   <  1:109679/239‑1 (MQ=255)

     aGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                                   <  1:1228452/239‑1 (MQ=255)

         ggCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTGCTTTGAAGCGGTTGCGCAGCTGCCGCAGGCGACGCGCAACCAGCTGGTTGTCACGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTATCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  2:95596/238‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:587455/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:672538/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:764167/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  1:913578/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  2:1207342/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAAc                                                                                                                                                                                                                                <  2:281742/236‑1 (MQ=255)

           cGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGCCTGCTGGCGAAGTCA‑CTCGGTCCGCCGGGGGAACGTTTTATTGCTGCGACCAACgcg                                                                                                                                                                                                                             >  1:951894/1‑237 (MQ=255)

               tgatgatgaAGAGCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                                                                                         <  2:1025045/239‑1 (MQ=255)

               tgatgatgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACg                                                                                                                                                                                                                         <  1:1377571/239‑1 (MQ=255)

                 atgatgaAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCGGGCGCAGTCA‑CTCCCTCTGCCGGGGGAAAGGTTTATTGCTGGGGCCACCGTTAACggg                                                                                                                                                                                                                       >  2:714427/1‑237 (MQ=255)

                     tgaAGAACTGAAAGTGGCGCTAGGGTTAAACCCGGCTACCTCGACTACCAACAGCTGTTTGCTGCAGCAGATTTGCTACTACTTTGAAGATGTCGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGTGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCa                                                                                                                                                                                                                                  <  2:1426505/224‑1 (MQ=255)

                      gaagaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGt                                                                                                                                                                                                                  <  1:124942/239‑1 (MQ=255)

                        agaaCTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGc                                                                                                                                                                                                                <  1:861410/239‑1 (MQ=255)

                               aaaGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCCGCTTGCGCAGTCC‑CTCGGTCTGGCGAGGAAACGTTTTATTTCTTGGCCCCACCTGAACGa                                                                                                                                                                                                                        >  1:1426505/1‑224 (MQ=255)

                                    ggCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAGTCA‑CTCGGCCTGCCGGTGAAAAGTTTTTTTGCTGCGCGCAACGGGAACGATACCGGGCCACGTTTCCtt                                                                                                                                                                                                     >  2:574032/1‑237 (MQ=255)

                                    ggCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCt                                                                                                                                                                                                      <  2:1165161/237‑1 (MQ=255)

                                      cgcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGACA‑ATCGGTCTGCCGGGGGAACGTTTTTTTGCTGCGACCCACGTGAACGCTACCGTGCCACCTTTCCTGCa                                                                                                                                                                                                   >  1:748200/1‑238 (MQ=255)

                                       gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                                 <  2:146863/239‑1 (MQ=255)

                                       gcTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                                 <  1:1022823/239‑1 (MQ=255)

                                        cTAGGGTTAACCCCGGCTAACTCGATTACCATCAGCGGTTTGCTGGGGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCacg                                                                                                                                                                                                 <  2:1228447/238‑1 (MQ=255)

                                              ttAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                           <  2:779949/238‑1 (MQ=255)

                                              ttAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                           <  2:1221748/238‑1 (MQ=255)

                                               tAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAg                                                                                                                                                                                         <  2:442947/239‑1 (MQ=255)

                                                 aaCTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTg                                                                                                                                                                                       <  1:315381/239‑1 (MQ=255)

                                                 aaCTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGGGAAAAGTTTTTTTTCTTCGACCCACCGGGACGATAACGTGCCCCGGTTCCTCCacgaccggccggg                                                                                                                                                                                       >  1:821767/1‑231 (MQ=255)

                                                   cTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGt                                                                                                                                                                                     <  1:1011034/239‑1 (MQ=255)

                                                      ggCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                           >  1:747595/1‑230 (MQ=255)

                                                      ggCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTc                                                                                                                                                                                           <  2:747595/230‑1 (MQ=255)

                                                       gCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTTCTGGCGAAGTCA‑CCCGGTCTTCCGGTGAAAAGTTTTTTTTCTGCGACCAACGTGAAAGATACCAGGCCCCCGTTCCTCCACCACGGTcgcgtggcaa                                                                                                                                                                                  >  2:1347245/1‑229 (MQ=255)

                                                                aTTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGGTGCTGGCGGAGTCAGCCCGGCCGACCGGGGAAA‑GTTTTTTTTCTTGGACCGACCTGGACGATACCATCCCCCGGTTCCTTCTCGGCCGGCAGCTGTCACCCAAAGCgg                                                                                                                                                                         >  2:178708/1‑237 (MQ=255)

                                                                 ttAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                                       <  1:728520/239‑1 (MQ=255)

                                                                     cATCTGCCGTGTGCTGGCGCAGAGTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                                   <  2:1079406/239‑1 (MQ=255)

                                                                     cATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAgg                                                                                                                                                                   <  1:136076/239‑1 (MQ=255)

                                                                          gCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTTTTTCTGCGACCAACGTGAACGCTACCGGGCCACGTTTCCTGCACGACGGTCAGTGGGCACCCACAGCGACCCAGGCGCCg                                                                                                                                                              >  1:576906/1‑239 (MQ=255)

                                                                               ttGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATc                                                                                                                                                         <  1:504153/239‑1 (MQ=255)

                                                                                               gCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGAcc                                                                                                                                                                                                                                   <  1:523956/149‑1 (MQ=255)

                                                                                                  gctactTTGAAGCTCTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGGTGGTTGTCGCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGAGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGACGACGTTATCCAACGCGATGGACGTGAGt                                                                                                                                      <  2:951894/238‑1 (MQ=255)

                                                                                                   ctactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTc                                                                                                                                     <  1:59509/239‑1 (MQ=255)

                                                                                                    tactTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCg                                                                                                                                                                 <  1:1313167/210‑1 (MQ=255)

                                                                                                     actTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGGAGGCA‑CCCGGTCTGCCGGGGGAACGGTTTTTTTCTGCGACCCACGTGAAAGAAACCGTGCCCCGTTTCCTGCCCGCCCGTCAGGGGGTCCCCAAAACCACTCCAGCGACGTTTTCCAACCCGCTGGACGGGTGTCa                                                                                                                                    >  2:1251321/1‑238 (MQ=255)

                                                                                                     actTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAg                                                                                                                                   <  1:1003762/239‑1 (MQ=255)

                                                                                                         tGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGACGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGa                                                                                                                               <  1:206604/239‑1 (MQ=255)

                                                                                                         tGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGAAGTCA‑CTCTGTCTGCCGGTGAAACGCTTTTTTTCTGCGACCCACGTGAACg                                                                                                                                                                                                                         >  2:523956/1‑149 (MQ=255)

                                                                                                          gAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGGGAAAAGTTTTATTTCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGGCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGGGAGTCAGACGa                                                                                                                               >  1:787164/1‑238 (MQ=255)

                                                                                                                gTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGGCTGCTGGCGAAGGCC‑CCCGGTCTGCCGGGGAAACGTTTTATTTCTGCGACCAACGTGAACCATACCGTGCCCCGGTTCCTGCCCGACGGGCAGTGGGCACCCAAAGCGACCCAGGCGACGGTATCCCACGCGATGGACGGGAGGCAGCCGGACAACTg                                                                                                                        >  2:922243/1‑239 (MQ=255)

                                                                                                                   gcgcAGCTGCCGCAGGAGACGCGCAACCAGCTTGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGcc                                                                                                                     <  1:173860/239‑1 (MQ=255)

                                                                                                                   gcgcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGcc                                                                                                                     <  2:33458/239‑1 (MQ=255)

                                                                                                                     gcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTTCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCcgc                                                                                                                   <  1:275911/239‑1 (MQ=255)

                                                                                                                     gcAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCcgc                                                                                                                   <  2:206753/239‑1 (MQ=255)

                                                                                                                       aGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCcgcg                                                                                                                  <  1:1157679/238‑1 (MQ=255)

                                                                                                                           gCCGCAGGAGACGGACAGCCAGGTGGTTGTCTCGGTGCCAAGCGGAAACGTCGGCGCGTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCTGTCTGCCGGTGAAACGGTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTgg                                                                                                             <  2:834504/239‑1 (MQ=255)

                                                                                                                                      cgcgCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTc                                                                                                  <  2:1299399/239‑1 (MQ=255)

                                                                                                                                                      tggcgcGGTGCAAAGCGGAAACGTGGGCGGTTGGACGGCGGGTCGGGTGGCGAAGTCA‑CTCGGTCGGCCGGGGAAACGTTTTAGTGCTGCGACCAACGTGAACTATACCGGGCCACGTTCCCTGCACGGCGGTCAGTGGTCGCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGGGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGc                                                                                  <  2:275029/234‑1 (MQ=255)

                                                                                                                                                            ggggCCAAGCGGAACCTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGa                                                                            <  2:927205/236‑1 (MQ=255)

                                                                                                                                                            ggTGCCAAGCGGAAACTTCGGCGATTTGCCGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGa                                                                            <  1:373783/239‑1 (MQ=255)

                                                                                                                                                                 gaaGCGGAAACTTCGGCGAGTTGACGGCGGGTCTGCTGGCGATGTAA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACTTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAagag                                                                       <  2:681493/238‑1 (MQ=255)

                                                                                                                                                                 cAAGCGGAACCTTCGGCGATTTGACGGCGGGTGTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAATGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAagag                                                                       <  2:113142/239‑1 (MQ=255)

                                                                                                                                                                 cAAGCGGAAACTTCGGCGATTTGCCGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAagag                                                                       <  2:237218/239‑1 (MQ=255)

                                                                                                                                                                 cAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAagag                                                                       <  1:714427/239‑1 (MQ=255)

                                                                                                                                                                   aGCGGAACCTTCGGCGTTTGGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGGGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCt                                                                     <  2:143896/239‑1 (MQ=255)

                                                                                                                                                                   aGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCt                                                                     <  1:965964/239‑1 (MQ=255)

                                                                                                                                                                       gAACCTTCGGAGAGTTGGCGGCGGGTCTGCTGGCGGAGTCA‑CTGGGTCGGCCGGTGAAACCTTTTATTGCGGCAGCCAACGTGAACGATACCGTGCAGGGTTGCCTGCACGGCGTGCAGTGGTCACCCAAAGCGACTCAGTCGACGTTATCCAACGCGTTGGACGTGAGTCAGCCGAACAACTGGCCGCTTGTGGAAGCGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTggg                                                                  <  2:355700/238‑1 (MQ=255)

                                                                                                                                                                           cTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                             <  1:496802/239‑1 (MQ=255)

                                                                                                                                                                           cTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGACC‑CTCGGTCTGCCGGTAAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATg                                                             <  2:506498/239‑1 (MQ=255)

                                                                                                                                                                            gtgggCGATTGGGCGGGGGGCTGGCTGGCGAGGGCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGGGGTCGCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGTCAACTGAAAGAGCTGGGTTATg                                                             <  2:1347370/235‑1 (MQ=255)

                                                                                                                                                                                  gATTTGACGGGGGGTCTGCTGGCGAAGTTA‑CTCGGTCTGCCGGTCAAACGTTTTATTGCCGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGCCTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCg                                                       <  2:259970/238‑1 (MQ=255)

                                                                                                                                                                                  gATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAATCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCg                                                       <  1:1288822/238‑1 (MQ=255)

                                                                                                                                                                                                tCTGCTGGCGAGGGCA‑CGCGGTCTGCCGGTGAAACGTTTTATTGCTGAGACCAACGTGAACGATACCGTGCCACGTTCCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCGGGGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAcc                                         <  2:1310481/238‑1 (MQ=255)

                                                                                                                                                                                                gccgctgGCGAAGTCC‑CCCGGTCTGCCGGTGAAACGTTTTATTGCTGCGCCCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACGCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAAcc                                         <  2:821767/235‑1 (MQ=255)

                                                                                                                                                                                                 ctgctgGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCAc                                       <  1:1522407/239‑1 (MQ=255)

                                                                                                                                                                                                 ctgctgGCGAAGTCA‑CACGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCa                                        <  1:905677/238‑1 (MQ=255)

                                                                                                                                                                                                  tgctgGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGa                                                                            >  2:667039/1‑201 (MQ=255)

                                                                                                                                                                                                  tgctgGCGAAATCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGGGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTTGGTTATGCAGCCGTGGATGAAGAAACCAc                                       >  2:984771/1‑238 (MQ=255)

                                                                                                                                                                                                     tgGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCaa                                   <  1:803635/239‑1 (MQ=255)

                                                                                                                                                                                                      ggCGAAGGCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAAc                                  <  1:61040/239‑1 (MQ=255)

                                                                                                                                                                                                        cGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGGGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACa                                 <  2:1517198/238‑1 (MQ=255)

                                                                                                                                                                                                         gAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAg                                >  1:174422/1‑238 (MQ=255)

                                                                                                                                                                                                          aaGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGAc                              >  1:558453/1‑239 (MQ=255)

                                                                                                                                                                                                                 cacgggcgcccGGTGAAACGTTGTATGTCTGCGCCCAACGTGACCGATACCGTGCCACGTTTCCAGCACGACGGTCAGTGGTCCCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  2:748200/230‑1 (MQ=255)

                                                                                                                                                                                                                 cTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  1:999227/239‑1 (MQ=255)

                                                                                                                                                                                                                 cTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  1:1063755/239‑1 (MQ=255)

                                                                                                                                                                                                                 cTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCg                        <  1:1107840/239‑1 (MQ=255)

                                                                                                                                                                                                                  tCGGTCTGCCGGTGAAACTTTTTATTGCGGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCCCCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGt                       <  2:1265778/239‑1 (MQ=255)

                                                                                                                                                                                                                  tCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGt                       >  2:1500253/1‑239 (MQ=255)

                                                                                                                                                                                                                      tCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                                       >  1:203107/1‑91 (MQ=255)

                                                                                                                                                                                                                      tCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                                       <  2:203107/91‑1 (MQ=255)

                                                                                                                                                                                                                              tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACt                                                                                                                                                                       <  1:671617/83‑1 (MQ=255)

                                                                                                                                                                                                                              tGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  1:743729/1‑238 (MQ=255)

                                                                                                                                                                                                                               gAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGaa           <  1:295628/238‑1 (MQ=255)

                                                                                                                                                                                                                               gAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGaa           <  1:1251321/238‑1 (MQ=255)

                                                                                                                                                                                                                                aaaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  1:1513440/1‑236 (MQ=255)

                                                                                                                                                                                                                                aaaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  2:295628/1‑236 (MQ=255)

                                                                                                                                                                                                                                aaaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  1:741209/1‑236 (MQ=255)

                                                                                                                                                                                                                                aaaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGa            >  2:169575/1‑236 (MQ=255)

                                                                                                                                                                                                                                 aaCGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTg        <  1:1092119/239‑1 (MQ=255)

                                                                                                                                                                                                                                    gTTTTATTGCTGCGACAAACATGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCAAAAGAGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGc     <  1:900835/239‑1 (MQ=255)

GGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCA‑CTCGGTCTGCCGGTGAAACGTTTTATTGCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGTCAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGCCGCAAAATCTGGCAACTGAAAGAGCTGGGTTATGCAGCCGTGGATGATGAAACCACGCAACAGACAATGCGTGAGTTAAAAGAACTGGGCTAC  >  NC_000913/4309‑4777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`