Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,484	C→A	13.8%	Q8K (CAA→AAA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,484	0	C	A	13.8%	212.2 / 3.8	92	Q8K (CAA→AAA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (29/49);  new base A (11/3);  total (40/52)
Fisher's exact test for biased strand distribution p-value = 6.85e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCG  >  NC_000913/3206254‑3206719                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  cATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGA‑GAATTTTTTCAGCGCAAAACAAAAACATGGGTTTCCCTGATGAGCG‑ACAGTAAAAAACAACAAGcc                                                                                                                                                                                                                                           >  1:1296097/1‑238 (MQ=255)  aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGGGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCa                                                                                                                                                                                                                                           <  1:65630/238‑1 (MQ=255)  aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                             <  1:1259295/236‑1 (MQ=255)  aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCa                                                                                                                                                                                                                                           <  1:234483/238‑1 (MQ=255)  aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCa                                                                                                                                                                                                                                           <  2:310669/238‑1 (MQ=255)      ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAAAAATAAAAAAAATGGAGTTGCCATGATGGGCG‑AAAATAATAAGCAACAAGAAATGaa                                                                                                                                                                                                                                      >  1:853033/1‑239 (MQ=255)      ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGG‑TGATTTTTCACGACAAATACAAAAAAAAGAGGTTACGTAGTGGGCG‑GAAGGAATAAGAAACATGCAGTGaa                                                                                                                                                                                                                                      >  1:51345/1‑239 (MQ=255)      ttAATTATCCGCAGTCGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTTAAACAAAAACAAAAAAAGGAGTTGCCATGATGAGCG‑AAAAGAAAAAAAAACAAGCAgggga                                                                                                                                                                                                                                      >  2:1218887/1‑235 (MQ=255)       tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                                      <  1:1218887/238‑1 (MQ=255)       tAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGC‑GGATTTTTTAAGACAAAAAAAAAAAATGGAGGTGGCAAGGTGTGGGAAAAGTATTAAACaaaggacatgaat                                                                                                                                                                                                                                      >  1:421644/1‑228 (MQ=255)                    ttGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                                                                                            <  1:1106259/219‑1 (MQ=255)                     tgtgATAAGCGCAGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTGGCGTAATATTCATTCTTATTTTCGCGTTGACTCACGAACTGTTAATTACATATTCACGGTGGCAAAATATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAgaga                                                                                                                                                                                                                      <  2:195591/238‑1 (MQ=255)                       tgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATACAAAAAAAGGAGTTGCCATAGTTGGCG‑AAAGAAATAAACAAAAGACAATGAAT‑AAATTA‑ACAGAGATTg                                                                                                                                                                                                                   >  2:1499789/1‑239 (MQ=255)                           aaGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                                                                                             <  1:1255694/211‑1 (MQ=255)                                   tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATAAAAAAAATGGAGGTGCCCTGGTGAGGG‑GAAGTAATAAAAAACAAGCAGTGAAT‑AAAGTG‑TAAAAGATTGTCGCT‑AAAttt                                                                                                                                                                                                       >  1:1231640/1‑238 (MQ=255)                                   tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATGGAGTTTCCATGATGGGGG‑GAAGTAATAAGAAACAAGCAGTGAAA‑AAAATG‑ACAGAGATTGTTGCT‑TACttt                                                                                                                                                                                                       >  1:267806/1‑238 (MQ=255)                                          cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTTAGACAAATACAAAAACCGGAGTCGCCATACTTGGCG‑GAAGTAATAAAAACCAAGCAGAGGAT‑AAAATG‑TAAGACATTATCCCC‑AACATT‑TCCGCCaa                                                                                                                                                                                              >  1:1450575/1‑238 (MQ=255)                                               aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGCTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑CAATTTTTTTAGACAGAAACACAAACTACAGGTGTCCTGATGAATG‑AAAGAAATAAGAAACAAGCAGTGAAT‑AAGTTG‑ATAGAGGTTATTGAC‑GACTTT‑TCCGTCATGAttt                                                                                                                                                                                         >  1:1495060/1‑239 (MQ=255)                                               aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCATACAAATACAAAAAAAGGGGTTTCCATTGTGAGGG‑AAAGAAAAAAAAAAAAGGAACAGAAT‑AAGATG‑ACATAGATTTTCGCT‑AACTTT‑ACCCCCCTGGttt                                                                                                                                                                                         >  2:1435656/1‑239 (MQ=255)                                                    tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACCAATACAAAAAATTGAGTTGCCATGATGAGCG‑AAAGTAATAAACAACAGGGCGGGAAT‑AAGATT‑ACAGAGATTGTTGGT‑CAATTT‑TACGCCATGCttttttc                                                                                                                                                                                     >  1:732931/1‑234 (MQ=255)                                                           cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAACAAAAAATTGAGGTGTCATGATGAGCG‑GAAATAATAAGCAAAAAGCAGTGAAT‑AAGTTG‑AAAGGGATTGTCGCT‑AAATTT‑ACCCACATGATTTTTA‑CCCGAATg                                                                                                                                                                             >  1:871984/1‑238 (MQ=255)                                                              ttcttgggCGAAATATTCACTTTTATTTTAGAGTTGATACACGAACTGTAAATTACATATCCGCGGTGAAAAAAAATATAAAACGACATTTTAAGTGGTAGTTTGTGGGGGT‑GATTTTTTCCAGACAAATAAAAAAACTGAATTTGCCATGATGAGCA‑AAAGAAATAACCAACAGGCATTGAAT‑AAGTTG‑ACAGAGATTGGCGCT‑ATCTTG‑ACCGCCATGATTTCTA‑CCCGAATGCCtg                                                                                                                                                                         <  2:55924/233‑1 (MQ=255)                                                                 tctGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑ATTTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCtgatg                                                                                                                                                                      <  1:734121/239‑1 (MQ=255)                                                                     gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAAAAAAAAAAATGGGGTTGCCATGATGAGCG‑AAAGTAATAAACAACAAGCAGTGAAA‑AAATTG‑AACGAGATTGTCGCG‑TACATT‑TCCCCCATTATTTTTA‑CCCCAAAGACTCATTACgt                                                                                                                                                                  >  1:59752/1‑239 (MQ=255)                                                                      cgcAATATTAATTTTTATTTCAGCGTTGATTCACAAACTGTAAATTACATATTCAGGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACgt                                                                                                                                                                  <  2:187344/238‑1 (MQ=255)                                                                       gaaaaattcatttTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GATTTTTTCAAGACAAATACAAAAACTGGAGTTGCTATGATGAGCG‑AAAGTAATAAGCAACAGCCAGTAATT‑AAGTTG‑ACAGAGATTGTCGCT‑ACCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                                 <  1:1111782/233‑1 (MQ=255)                                                                       gaaaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCAAGACAAAAACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACgtg                                                                                                                                                                 <  1:1499789/236‑1 (MQ=255)                                                                           tatTCATTTTTTTTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAACCCACATTTTTAGTGGTATTTTGTGGCGGT‑AAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCAATGATGAGCG‑AAATTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATGGTCGTT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  2:1225370/239‑1 (MQ=255)                                                                           tatTCATTTTTATTTTCGCTTTGATTCACGAATTTTTAATTACATATTCAGGGTGGAAAAAAATATAAAACCAAATTTTTAGTGGTGGTTTGTGGCGGT‑GAATTTTTCCAAAAAAATAAAAAAACGGGGGTTGCCATGATGAGCA‑AAAGTAATAAGCAACGGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  2:1231640/239‑1 (MQ=255)                                                                           tatTCATTTTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGGGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGT‑AAATTTTCCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCATTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  1:1435656/239‑1 (MQ=255)                                                                           tatTCATTCTTATTTGCGCGTTGATTCACAAACTGTTAATTAAATATTCACGGGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAAAACAAAAACTGGAGTTGCCATGATAAGCG‑AAAGTAATAAGCAACGGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  1:1285598/239‑1 (MQ=255)                                                                           tatTCATTCTTAATTTCTCTGTAATCCACGAATTGTTAATTACAGATTCACGGTGGCAAAAAATATAAAACCACATTTTTGTGGGTAGTTTGTGGGGGT‑GAATTTTTCCAAACAAATACAAAAACTGGTGTTGCAATGGTGAGCG‑AAAATAATAAGCACCAGGCAGTGAAT‑AAGTTG‑ACATTGATTGACGCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  2:1495060/239‑1 (MQ=255)                                                                                    ttATTTTCGCGTTGATTCACGAATTGTTAATTACATATTAACGGAGGCAAAAAAAATAAAACCACATTTGTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAAGTGGAGTTGCCATGATGAGAA‑AAAAAAATAAGCAACAGGCAGTAAAG‑AAGTTG‑ACAGAGATTGTCGCT‑ATCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                   <  1:869/239‑1 (MQ=255)                                                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATGGTGAGCG‑AAAGTAAAAAAAAACAAGCAAGGAAT‑AAAATG‑ACAGAGATTGTTGCT‑AAATTT‑ACCCCCATGATTTTTA‑TCCCAAAGGCTGATGACGGGGGGGGtaaaataaaa                                                                                                                                                  >  1:208935/1‑239 (MQ=255)                                                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGAAAAATACAAAAAATGGGGTTTCCATGATGAGCG‑AAAGAAAAAAACAAAAAGCAGAGAAA‑AAAATG‑AAAGAAATTGTTGCT‑AAACTT‑TCCGCCCTGATTTTTA‑CCCCAAATCCTGATGAAGcggtggggaaaaaaaaa                                                                                                                                                  >  1:87149/1‑228 (MQ=255)                                                                                           cgcgTTGATTCACGAACTGTTAATTACATATTCGCGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑AAATTTTTCCAGAAAAATACAAAAACTGGATTTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa                                                                                                                                            <  2:445209/239‑1 (MQ=255)                                                                                            gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAATAAAAAAAATGGAGTTGGCATGATTAGCG‑GAAGAAATAAACAACAAGGAGAGAAT‑AAAATG‑AGAGAGAGTGTTGCT‑AAATTT‑ACCCCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGGGGATAAAATAAAACAGCtca                                                                                                                                           >  1:567210/1‑237 (MQ=255)                                                                                             cgTTGATTATCAATCTGTTAATTGCTTCATAAGGGAGGAAAAAAATATAAAACCACATTTTTATTGTTAGTTGTTGGCGGT‑TAATTTTACTAAACAAATCAAAAAATTGGATTGGCCAGGATGACAG‑AAAGAAACAGGAAAAAGGCCGTAAAT‑AAGTTG‑CCAGAGATTGTCGCT‑ATTTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa                                                                                                                                          <  1:1181395/239‑1 (MQ=255)                                                                                                       cGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTGGTGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCAATGATGAGCA‑AAAGAAATAAGCAACAGGTAGTGAAT‑AAGTTG‑AAAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                <  1:894167/239‑1 (MQ=255)                                                                                                        gaccttttAATTACATTTTCACGGTGGAAAAAAATATAAACCCACATTTTTAGGGGTAGTTTGGGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAAAAAGCAACAGGCAGTGAAT‑‑AGTTGAACAGAGATGGTCGCT‑ACCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaaa                                                                                                                               <  1:31673/233‑1 (MQ=255)                                                                                                            tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAAAAAAAAAAATGGGGGTGTCATGATGAGGG‑GAAAAAATAAACAAAAGGAAGTGAAT‑AAGTTG‑AAAGAGAATGTCGCTAAAATTT‑ACC‑CCATGATTTTTA‑CCCGAAAACCTGCTGACGTGGTGGATAAAAAAAAAAAGCTAAAAGATGCCGacaacg                                                                                                                            >  1:425549/1‑233 (MQ=255)                                                                                                              ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCACACAAAAACAAAAAAAGGAGTTGCCCTCATGAGGG‑GAAAAAAAAAAAAAAAAGCAGCGAAA‑AAGTTG‑AAAGAGATTGTGGCT‑AACTTT‑ATACCCATGATTTTTT‑ACCCAATGCCTGTTGTAGGGGTGGGGAAACCAAAAAAGATTAAAGAAGGCGaaaattgg                                                                                                                          >  2:819420/1‑233 (MQ=255)                                                                                                              ttAATTACATATTCACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGAAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  1:425536/204‑1 (MQ=255)                                                                                                                      atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAAAAAAAAAACTGGAGGTGTCTTGATGAGGG‑GAAGTAAAAAACAAAAGGGAGAGAAT‑AAGTTG‑ACAGAGATTTTCGCT‑AACTTT‑ACCGCCCTGATTTCTT‑CCCGCATGCCTGATGACGTGGTGGATAAACTAAAACAACTAAAGAATGCCGAAACGACGTC‑GGAgggg                                                                                                                >  2:91766/1‑239 (MQ=255)                                                                                                                       tatgccgggTGGCAAAAAAGAAAAAACCACATTTTAAGGTGTTGTTGGTGGCGTG‑TAATTTTTCCAGACAAACACAAAAACTGGAGTTGCCATGATGAGGT‑AAAGAAAAAAGCAACAGGCAGTTAAA‑AGTTTG‑ACAGAGTTTGTGGCT‑ATCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                               <  2:903177/232‑1 (MQ=255)                                                                                                                       tatTCGCGGTGAAAAAAAATAAAAAACCAAATTTTAAGTGGTAGTTTGTGGCGGT‑GAATTTTCCAAGACAAAAAAAAAAACGGGAGTTGCCTGGATGAGCG‑AAAGTAATAAGCAAACGGCAGTGAAT‑AATTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                               <  2:687815/239‑1 (MQ=255)                                                                                                                       tatTCACGGGGGCAAAAAATATAAAACAACATTTTAAGGGGTAGTTTGTGGGGGT‑GAATTTTCCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                               <  1:323688/239‑1 (MQ=255)                                                                                                                       tatCCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGT‑AAATTTTCCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGa                                                                                                               <  1:813275/239‑1 (MQ=255)                                                                                                                               tgggaaaaaaaaaTAAAACCACTTTTTTAGTGGTAGTTTGGGGGGGG‑GAATTTTTACAGACAAATACAAAAACTGGAGGTGCCATGATGAGGG‑AAAGTAATAAGCAACAGGCATTAAAT‑AAGTTG‑ACAGAGATTTCCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                        <  2:853033/233‑1 (MQ=255)                                                                                                                               tgggaaaaaaaTAAAAAACCCAATTTTAAGTGTGAGTTTGTGGGTGT‑AAATTTTCCCAGACAAATAAAAAACCGGGAGTTCCCTGGAGGAGGG‑AAATTAATAAGCAAAAGACATTAAAG‑AAGTGG‑ACAGAGATTGTCGCT‑ATCTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                        <  2:513031/233‑1 (MQ=255)                                                                                                                               ggggaaaaaaaTATAAAACCACATTTTTGGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTGG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                        <  2:267806/233‑1 (MQ=255)                                                                                                                               gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGGGTTGCCATGATGAGCG‑GAAGTAATAAAAAACAAGCAGAGAAT‑AAATTG‑ACAGAGATTGTCGCT‑AACTTT‑TCCGCCATGATTTTTA‑CCCCAAAGCCCGATGACGGGGGGGGTAAAATAAAACAGCTAAAAGATGTCGCAAAGTCGCC‑GGGGGGGGAAATTa                                                                                                         >  1:513765/1‑237 (MQ=255)                                                                                                                               gTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGTGGGGGT‑GAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTAt                                                                                                        <  1:1389957/238‑1 (MQ=255)                                                                                                                                tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCCGACAAAAACAAAAACTGGAGTTTCCATGATGAGCG‑AAAGTAAAAAACAACAGGCAAAGAATAAAGTTG‑‑CAGAGATTGTCGCT‑AAATTT‑ACCGCCATGATTTTTT‑CCCCAATGCCCGATGAAGTTGGGGGTAAAATAAAAAAACTAAAAGGTGCCGAAAACGCGTC‑GGTGGGGGAAAATATCt                                                                                                      >  2:224749/1‑239 (MQ=255)                                                                                                                                tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAAACAAAAAAAAAAAATGGAGGTGTCATGATGAGCG‑GAAATAATAAACAAAAAGCAAGGAAT‑AAAATG‑ACAGAGATTGTTGCT‑AACTTT‑ACCGCCATGATTTTTACCCCCAATG‑CTTATGGCGGGGGGGGTAAAACAAAACAGCTAAAAGGAGCCGAAAAGGCCTCGGAGGGGGAAAAtatat                                                                                                       >  2:628479/1‑235 (MQ=255)                                                                                                                                 agaaaaaaaTATAAACCCACATTTTTAGTGGTAGTTTGGGGCGGT‑AAATTTTTCAAAACAAAAACAAAAACTGGAGTTGCCATGATGAGGA‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGTT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTa                                                                                                     <  2:348892/236‑1 (MQ=255)                                                                                                                                  gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAAATTTTTCAAACAAATAAAAAAAATGGAGTTGGCATGATTAGCG‑AAAGGAATAAAAAAAAGGCAGGGAAT‑AAATTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCCTGATTTTTA‑CCCCAAAACCTGATGAAGTGGGGGGTAAAATAAAACAACTAAAAGAAGCCCAAAACTCGTC‑GGTGGGGGAAAATATTTa                                                                                                     >  1:405616/1‑238 (MQ=255)                                                                                                                                   cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGCG‑GAAGTAAAAAACAAAAAGCAGAGAAT‑AAAATG‑ACAAAGATTGTCGGC‑AACTTT‑TACACCCTGATTTTTT‑CCCCGATGGCTCATGAAGTGGGGGGTAAAATAAAAAAACGAAAAGAGACCGAAAAGTCGCC‑GGTGGGGGAAATTATATAcc                                                                                                   >  2:1389957/1‑239 (MQ=255)                                                                                                                                      aaaaTATAAACCCAAATTTTAAGTGGTAGTTTGGGGCGGT‑AAATTTTCCCAAAAAAATAAAAAAACTGGATTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATa                                                                                                <  1:303345/239‑1 (MQ=255)                                                                                                                                        aaTATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                              <  1:856817/239‑1 (MQ=255)                                                                                                                                        aaTAAAAAACCACAGTTATAGTGGTAGTTTGTGGCGGT‑AAATTTGCCAAGAAAAAAACAAAAACGGGAGTTGCAATGATGAGCA‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCTCA‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACg                                                                                              <  2:732931/239‑1 (MQ=255)                                                                                                                                           ataAACCCAAATTTTGAGGGGTATTGTGTGGGGGT‑GAATTTTGCCAAAAAAATACAAAAACTGGATTTGCTATAAGGAGCG‑AAAGTAAAAAACAACAGGCAGTGAAA‑AAGTTG‑ACAGAGATTGTCGAT‑ACCTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATg                                                                                           <  2:1296097/239‑1 (MQ=255)                                                                                                                                            taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCC‑GACAAAAAAAAAAAATGGAGTTGGCATGTTGGGGG‑GAAAAAATAAACAAAAAGCAGTGGAT‑AAAATT‑AAAGAAAATGTTGCT‑AAATTTAACCCCCAT‑ATTTTTA‑CCCCAATGCCCGATGACGGGGGGGGGAAAAAAAAAAAGCTAAAAGATGGCGGAACATCGGC‑GGTGGGGGAAAATATATAACATAcaaagt                                                                                          >  2:837292/1‑234 (MQ=255)                                                                                                                                                    caTTTTTAGTGGTAGTTTGTGGCGGT‑GGATTTTTTCAGACAAAAACAAAAAATGGGGTTGCCATGATGAGGG‑AAAGAAATAAGCAACAGGCAGTGAAA‑AAATTG‑AAAGAGATTGTCTCT‑AACTTT‑ACCGCCCTGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGGTAAAATAAAAAAGCTAAAGGATGCCGAAAAGTCGTC‑GGTGGGGAAAATTATATAACATCAGATTTTCGacaac                                                                                  >  1:1188013/1‑239 (MQ=255)                                                                                                                                                             tgggattttGTGGGGGT‑GAATTTTTCCAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa                                                                          <  2:712410/232‑1 (MQ=255)                                                                                                                                                                 ggttggggggggT‑AAATTTTACAAAAAAAATAAAAAACCTGGAGTTGCAATGATGAGCG‑AAATAAATAAGCACAAGGCAGTAAAT‑AAGTTG‑ACAGAGTTTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                     <  2:425549/229‑1 (MQ=255)                                                                                                                                                                 aGTGTGTGGTGGT‑GAATTTTTCCAAAAAAATACAAAAACTGGAGTTGCTATGATGAGCG‑AAAGTAATAAGCAACGGGAAGGTAAT‑AAGTTG‑AAATAGATTGTCGTT‑CACTTT‑TCCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                     <  1:779026/239‑1 (MQ=255)                                                                                                                                                                       gtgcatgtaaaTTTTACCAAACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTATAAAGCAACAGGCAGTAAAT‑AAGTTG‑AGAAAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAc                                                                <  1:382967/230‑1 (MQ=255)                                                                                                                                                                                    ttccaaGAAAAATACAAAAATTGAAGTTGCAATGAGGGGAG‑AAAGAAAAAACCAACAGGCAGTGAAT‑AATTTG‑ACAGAGATTGTCCAT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                   <  1:579119/234‑1 (MQ=255)                                                                                                                                                                                          gACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTc                                             >  2:679607/1‑239 (MQ=255)                                                                                                                                                                                           aaaaaaaaaaaaaCTGGAGTTGCAATGATGAGCA‑AAAGTAAAAAGCAAAAGGAAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcc                                                     <  2:1222728/222‑1 (MQ=255)                                                                                                                                                                                                   aaaaaaTGGAGTTGCCATGATGAGCT‑AAAGTAATAAGCAACAGGAGGTGAAT‑AAGTTG‑ACAGATATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                                    <  2:82481/239‑1 (MQ=255)                                                                                                                                                                                                     aaaCTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAg                                           >  1:1222728/1‑230 (MQ=255)                                                                                                                                                                                                     aaaCTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                  >  1:819506/1‑239 (MQ=255)                                                                                                                                                                                                     aaaCTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                                  >  1:951176/1‑239 (MQ=255)                                                                                                                                                                                                      aaCTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGAAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGAT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                 <  1:1465131/239‑1 (MQ=255)                                                                                                                                                                                                      aaCTGGAGTTGCCAGGAGGAGCG‑AAAGAAAAAAGCAACAGGCAGTGAAT‑AAGTTG‑AAAGAGATGGCCGCT‑AACTTA‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                 <  1:454149/239‑1 (MQ=255)                                                                                                                                                                                                      aaCGGGAGTTGCAATGAGAAGGA‑AAAGTAATAAGCAACAGGCAGGGAAT‑AAGTTA‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                 <  2:513765/239‑1 (MQ=255)                                                                                                                                                                                                       aCTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCGTACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                >  1:347329/1‑239 (MQ=255)                                                                                                                                                                                                       aCTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                                >  1:1512087/1‑239 (MQ=255)                                                                                                                                                                                                                gCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCACAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                        >  2:93872/1‑238 (MQ=255)                                                                                                                                                                                                                gCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                        >  2:1114524/1‑238 (MQ=255)                                                                                                                                                                                                                gCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGt                        >  1:1294000/1‑238 (MQ=255)                                                                                                                                                                                                                gCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTAAAT‑AAGTTG‑ACAGAGATTGTCGTT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                       <  1:463405/239‑1 (MQ=255)                                                                                                                                                                                                                  cATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGttct                     <  1:837292/239‑1 (MQ=255)                                                                                                                                                                                                                           cG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg            >  1:574027/1‑239 (MQ=255)                                                                                                                                                                                                                           cG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg            >  1:175359/1‑239 (MQ=255)                                                                                                                                                                                                                           cG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg            >  1:961929/1‑239 (MQ=255)                                                                                                                                                                                                                           acaaaaGTAAAAAGCAACAGGCAGTAAAT‑AAGTTG‑AAAGAGATTGTCTCT‑AATTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaaa             <  1:819420/236‑1 (MQ=255)                                                                                                                                                                                                                             aaaaGTAAAAAGAAACAGGCAGTGAAT‑AAGTTG‑AAGGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaaa             <  2:405616/236‑1 (MQ=255)                                                                                                                                                                                                                                aGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCgg        >  1:958413/1‑239 (MQ=255)                                                                                                                                                                                                                                      aaGCAACAGGCAGGAAAA‑AAGTTG‑AAAGATATTGCCGCT‑AAATTC‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg  <  2:306282/239‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  CATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGT‑GAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTG‑ACAGAGATTGTCGCT‑AACTTT‑ACCGCCATGATTTCTA‑CCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTC‑GATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCG  >  NC_000913/3206254‑3206719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑